Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,094,8080AC47.6% 18.4 / 10.4 44L186R (CTG→CGG) CJ019_01995hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (11/11);  new base C (12/8);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 5.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAAT‑ACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCAAG  >  ACB143/2094686‑2094936
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ccATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAAT‑ACTGCACGCCGCGCTCGAAGGCCCCGCCCGCCGGGCCg                                                                                                                    >  1:15433/1‑137 (MQ=255)
ccATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAAT‑ACTGCACGCCGCGCTCGAACGCCCGGCCCCCCAGGccc                                                                                                                    >  7:202930/1‑136 (MQ=255)
ccATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCGCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAAT‑ACTGCACGCCGCGCTCGACGGCCAGGCGCGCCTGGGCg                                                                                                                    >  4:252080/1‑137 (MQ=255)
 cATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAAT‑ACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGGCCgg                                                                                                                   >  6:112593/1‑137 (MQ=255)
     cGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAAT‑ACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCAGGCCGGGcgg                                                                                                               >  7:288727/1‑135 (MQ=255)
     cGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAAT‑ACTGCACGCCGCGCTCGAAGGCCCCGCGCGCccggcccggcgg                                                                                                               >  1:179099/1‑135 (MQ=255)
                  gggCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAAT‑ACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAgg                                                                                                  >  4:217499/1‑137 (MQ=255)
                   ggCCTTGTCCCGGCACAAGAGATTCGCAAGACTCCTCAGCGGCTCTTCATACCCCTTCGGCGCCTGTTGCTGGCGCAAAT‑TCCCCACGCCGCCGTCGATGGCCACGCGCGCGTGGCCGGGCTGGCCTTTTTGCAggg                                                                                                 <  8:184381/137‑1 (MQ=255)
                     cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAAT‑ACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCAGGCCGGGCGGGCCGTTTTTCAGGGcc                                                                                               >  1:55226/1‑137 (MQ=255)
                     cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAAT‑ACTGCACGCCGCGCTCGAAGGCCCGGCCCGCCGGGCCGGGCGGGGCGGTTTGAAGGGcc                                                                                               >  1:119589/1‑137 (MQ=255)
                     cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAAT‑ACTGCACGCCGCGCTCGAAGGCCCCGCGCGCCGGGCCCGGCGGGGCGTTTTGCAGGGcc                                                                                               >  3:23915/1‑137 (MQ=255)
                     cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAAT‑ACTGCACGCCGCGCTCGAAGGCCCCGCGCGCCAGGCCGGGGTGGGCGTTTTGGCGGGcc                                                                                               >  1:51448/1‑137 (MQ=255)
                                           ctcAAGATCCGTCCGCGGCTCTCAAAACCCCTTCGCCTCCGGCGGCTTGCGAAAAA‑CCTCCACCCCGCGCGCCACGGCCCGGCGCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTgaaga                                                                         <  2:78019/137‑1 (MQ=255)
                                           ctcAAGAGACGGCAGCGCCGCTTCAACCCCCTGGGGCGCCGGCGGCTTCCGCAAAT‑CCTGCACGCCGCGCGCGACGGCCAGGCGCGCGTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTgaaga                                                                         <  6:117994/137‑1 (MQ=255)
                                             cAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAAT‑ACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGGGCCGGGCTGGCCGTTTTGCAGGGCCGGTTGGCCGAGGTTGTTGAAGTAc                                                                       >  2:203737/1‑137 (MQ=255)
                                             cAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAAT‑ACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGGCTTTTTGCAGGGCGTGTTGTCCGAGGTTGTTgaagaaa                                                                       >  7:288665/1‑136 (MQ=255)
                                             cAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAAT‑ACTGCACGCCGCGCTCGAAGGCCACGCCCGCCTGGCCCGGCTGGCCGTTTTGCAGGGCCAGTTTGCCGAGGGTTTTGAAGAAc                                                                       >  6:171164/1‑137 (MQ=255)
                                                     gaccgctcctCTTCAAACCCCTTCGGCTCGGGCTGCTTGCGAAAAT‑ACCGCCCGCGGCGCCCGACGGCCCGGCGCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATg                                                               <  1:38318/133‑1 (MQ=255)
                                                      tccgctgctCTTCATACACCTTCGGCGCGGGCGGCTTCCCAAAAT‑CCGCCCCGCCGCGCTCGCCGGCCCGGCGCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgc                                                              <  1:10818/134‑1 (MQ=255)
                                                      ccAGCGGCTCTACATCCCCCTGCGGCGCCGGGGGCTT‑CCCAAATAACGGCACGCCGCGCGCGACGGCCAGGCGCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgc                                                              <  7:123835/136‑1 (MQ=255)
                                                           tgctCTTCATACACCTTGGGCTCGGGCTGCTTGCTAAAAT‑ACTCCACGCCGCGCGCGACGGCCCGGCGCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgcagcag                                                         <  3:134422/137‑1 (MQ=255)
                                                           tgctCTTAATACACGTTGGGCTCGGGCGGGTTGCGCAAAT‑ACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgcagcag                                                         <  4:23915/137‑1 (MQ=255)
                                                                          ttCGGCTCCGGCTGCTTGCGCAAAT‑ACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCg                                          >  6:255972/1‑137 (MQ=255)
                                                                          tgcGGCTCCGGCTGCTTGCGAAAAT‑CCTCCCCGCCGCGCGCGACGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCg                                          <  2:15433/135‑1 (MQ=255)
                                                                             ggCTCCGGCTGCTTGCGCAAAT‑ACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGGAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAt                                       >  2:25735/1‑137 (MQ=255)
                                                                               cgccGGCGGCTTGCGCAAAT‑ACCGCCCGCGGCGCTCGAGGGCCCGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAttt                                     <  3:90862/135‑1 (MQ=255)
                                                                               cTCGGGCTGCTTGCGAAAAT‑ACTCCCCGCCGCGCTCCAAGGCCAGGCGCGCGGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAttt                                     <  5:298483/137‑1 (MQ=255)
                                                                               cTCGGGCTGCTGGCCCAAAA‑ACTGCCCGCCGCGCTCGACGGCCTGGCGCGCGGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAttt                                     <  1:74746/137‑1 (MQ=255)
                                                                               cTCCGGCTGCTTGCGCAAAT‑ACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGGCGTTTTGCACGGCCTGTTGGGCGAGGTTGTTGAAGAACACTATATTCCGCAGCACCAGGATATGGCGGCttt                                     >  6:11159/1‑137 (MQ=255)
                                                                               cTCCGGCTGCTTGCGCAAAT‑ACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCAGGCCGGGCTGGCCATTTTGCAGGGCGTGTTGGCGGAGGTTGTTGAAGACATGTAGATTCCGCCGCACAAGTAAATGGCGGAgtt                                     >  6:304253/1‑137 (MQ=255)
                                                                               cTCCGGCTGCTTGCGCAAAT‑ACTGCACGCCGCGCTCGAAGGCCACGCGCGCCAGGCCGGGCTGGCCTTTTTTCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAttt                                     >  1:31258/1‑137 (MQ=255)
                                                                               cTCCGGCGGCTTGCGCAAAT‑ACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAttt                                     <  2:251664/137‑1 (MQ=255)
                                                                                     gtgctTGCGAAAAT‑ACTGCCCGCCGCGCTCGACGGCCCGGCGCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGa                               <  5:112593/136‑1 (MQ=255)
                                                                                      tgctTGCGCAAAT‑ACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGAc                              >  4:194033/1‑137 (MQ=255)
                                                                                      tgctTGCGCAAAT‑ACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGGTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGAc                              >  8:289687/1‑137 (MQ=255)
                                                                                          tgccaaaaT‑ACAGCACGCCGCGCCCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCaa                          <  8:205583/132‑1 (MQ=255)
                                                                                          tGCGCAAAT‑ACTGCACGCCGCTCTCGACGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCaa                          <  8:288727/137‑1 (MQ=255)
                                                                                          ggcgcAAAA‑CCTCCACCCCGCCCTCGCAGGCCAGGCGCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCaa                          <  6:292262/136‑1 (MQ=255)
                                                                                          ggcgaAAAT‑ACGGCCCGCCGCGCTCTCCGGCATGGCGCGCGTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCaa                          <  4:273060/136‑1 (MQ=255)
                                                                                                           gccgcGCTCGACGGCCAGGCGCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAg          <  6:120444/137‑1 (MQ=255)
                                                                                                                gcTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGCACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGc     >  8:254578/1‑137 (MQ=255)
                                                                                                                gcTCGAAGGCCACGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCGGTTGGGCGAGGTTTTTGAAGAACTCTATATGCAGCAGCACCAGGATATTGCGGCTTTCCTTGACCCAATTCTTGGCCTAGACAGCGGGc     >  4:49034/1‑137 (MQ=255)
                                                                                                                   cGACGGCCAGGCGCGCCGGGCCGGGCTGTCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag  <  8:276758/137‑1 (MQ=255)
                                                                                                                   cGAAGGCCCGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag  <  2:55226/137‑1 (MQ=255)
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CCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAAT‑ACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCAAG  >  ACB143/2094686‑2094936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑