| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB143 | 5,950,136 | Δ1 bp | 6.4% | coding (75/2244 nt) | ppk ← | Polyphosphate kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 5,950,136 | 0 | C | . | 6.4% | 219.5 / 7.4 | 62 | coding (75/2244 nt) | ppk | Polyphosphate kinase |
| Reads supporting (aligned to +/- strand): ref base C (23/35); new base . (2/2); total (25/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.86e-01 | |||||||||||
CGAGAGTTCGCGATGAATGTACAGGCTGCTGTCGTCCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCATTATTCATCTGGCGTTCCTGAGGACGTTAACGCCCTATCATCTATTGAGCGGCAAGATAAGCGCTCA > ACB143/5950001‑5950269 | cGAGAGTTCGCGATGAATGTACAGGCTGCTGTCGTCCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGc < 7:40633/136‑1 (MQ=255)cGAGAGTTCGCGATGAATGTACAGGCTGCTGTCGTCCAGGCCAGGGACGGCGATGGGCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGc < 6:122375/136‑1 (MQ=255) gTTCGCGATGAATGTACAGGCTGCTGTCGTCCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGt > 7:310369/1‑136 (MQ=255) cgATGAATGTACAGGCTGCTGTCGTCCAGGCCAGGGACGGCGATGGTAGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTgca < 1:303449/136‑1 (MQ=255) cAGGCTGCTGTCGTCCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCtcttct < 8:139805/136‑1 (MQ=255) cAGGCTGCTGTCGTCCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCtcttct < 3:371834/136‑1 (MQ=255) cAGGCTGCTGTCGTCCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCtcttct < 5:130866/136‑1 (MQ=255) ctgTCGTCCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCGGCAGCATCTCTTCTGGGAGCt > 7:261062/1‑136 (MQ=255) gtcgtcCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTcc > 1:189153/1‑136 (MQ=255) gtcgtcCAGGCCAGGGACGGCGAAGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTcc > 8:152137/1‑136 (MQ=255) aggccaggGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCAt > 6:237201/1‑136 (MQ=255) gccaggGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATcc > 1:97688/1‑136 (MQ=255) catggACGGCGAGGGGCGGGGACGGCGCGGGCACCGCAGCCACATCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCtt < 8:361434/133‑1 (MQ=255) caggGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCtt < 2:97444/136‑1 (MQ=255) aCGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGc > 8:343075/1‑90 (MQ=255) aCGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGc < 7:343075/90‑1 (MQ=255) aCGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATcg < 5:240981/136‑1 (MQ=255) aCGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATcg < 5:237201/136‑1 (MQ=255) ggCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGAGCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATGTCTTCTGGGAGCTCCTGAGCATCCTTGATcgcg < 6:49138/136‑1 (MQ=255) ggCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATcgcg < 1:122467/136‑1 (MQ=255) ggCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATcgcg < 3:384514/136‑1 (MQ=255) ggCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGGCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATcgcg < 7:141782/136‑1 (MQ=255) aTGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCATTGATCGCGACAg > 8:184057/1‑136 (MQ=255) tCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCt < 7:333678/136‑1 (MQ=255) ggggACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCt > 7:191268/1‑134 (MQ=255) ggggACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTa < 3:138822/136‑1 (MQ=255) ggggACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTa < 1:293546/136‑1 (MQ=255) ggggACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGG‑GGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCtt < 6:59626/118‑1 (MQ=255) ggggACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGG‑GGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCtt > 5:59628/1‑118 (MQ=255) ggggACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGG‑GGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCtt > 5:59626/1‑118 (MQ=255) ggggACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGG‑GGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCtt < 6:59628/118‑1 (MQ=255) gggACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAg < 5:301534/136‑1 (MQ=255) cgcgGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCa < 6:251101/136‑1 (MQ=255) gggCACCGGAGCCACCGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGCGCATCCTTGATCGCGCCAGGGCGTAGCACTTCatta > 2:131319/1‑136 (MQ=255) gggCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCCCCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTGCatta > 6:20729/1‑136 (MQ=255) gggCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCatta > 3:388363/1‑136 (MQ=255) gggCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCatta > 6:25486/1‑136 (MQ=255) cACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTGCTGGGAGCTCCGGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCcttatgc < 6:145420/136‑3 (MQ=255) aCCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCATTATTCa > 7:273689/1‑136 (MQ=255) ccTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCATTATTCATCTGGCGTTCCTg < 1:99112/136‑1 (MQ=255) caccTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCATTATTCATCTGGCGTTCCTGAGGACGt < 1:90414/136‑1 (MQ=255) ccTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCATTATTCATCTGGCGTTCCTGAGGACGTTa < 6:172185/136‑1 (MQ=255) ccTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCATTATTCATCTGGCGTTCCTGAGGACGTTa < 5:25486/136‑1 (MQ=255) ccTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCATTATTCATCTGGCGTTCCTGAGGACGTTa < 5:20729/136‑1 (MQ=255) ttCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCATTATTCATCTGGCGTTCCTGAGGACGTTAACGc < 2:280632/136‑1 (MQ=255) ttCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCATTATTCATCTGGCGTTCCTGAGGACGTTAACGc < 2:97688/136‑1 (MQ=255) ttCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCATTATTCATCTGGCGTTCCTGAGGACGTTAACGc < 5:125578/136‑1 (MQ=255) ttCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGGCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCATTATTCATCTGGCGTTCCTGAGGACGTTAAc < 7:178103/134‑1 (MQ=255) cGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCATTATTCATCTGGCGTTCCTGAGGACGTTAACGccc < 7:25259/135‑1 (MQ=255) cGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCATTATTCATCTGGCGTTCCTGAGGACGTTAACGCCCt < 1:175481/136‑1 (MQ=255) cAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCATTATTCATCTGGCGTTCCTGCGGACGTTAACGCCCTatca > 8:86701/1‑135 (MQ=255) gCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCATTATTCATCTGGCGTTCCTGAGGACGTTAACGCCCTATCATCTa > 3:95129/1‑136 (MQ=255) tCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCATTATTCATCTGGCGTTCCTGAGGACGTTACCGCCCTATCATCTAtt > 4:143770/1‑136 (MQ=255) tCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCATTATTCATCTGGCGTTCCTGAGGACGTTAACGCCCTATCATCTAtt > 5:42854/1‑136 (MQ=255) tCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTGGATCGCGACAGGGCTTAGCACTTCATTATTCATCAGGCGTTCCTGAGGACGTTAACGCCCTATCATCTAtt > 3:288852/1‑136 (MQ=255) ccACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCATTATTCATCTGGCGTTCCTGAGGACGTTAACGCCCTATCATCTATTGAGc < 2:107612/136‑1 (MQ=255) cACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCtt < 7:369242/60‑1 (MQ=255) cACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCtt > 8:369242/1‑60 (MQ=255) aCCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCtt < 2:282423/59‑1 (MQ=255) aCCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCtt > 1:282423/1‑59 (MQ=255) gAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCATTATTCATCTGGCGTTCCTGAGGACGTTAACGCCCTATCATCTATTGAGCGGCAAg < 6:90707/136‑1 (MQ=255) aGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCATTATTCATCTGGCGTTCCTGAGGACGTTAACGCCCTATCATCTATTGAGCGGc > 8:236639/1‑132 (MQ=255) cAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCATTATTCATCTGGCGTTCCTGAGGACGTTAACGCCCTATCATCCATTGAGCGGCAAGATaa < 7:216929/136‑1 (MQ=255) ggCGGGGTCTGCATCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCa < 8:82791/64‑1 (MQ=255) ggCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCa > 7:82791/1‑64 (MQ=255) ggCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCATTATTCATCTGGCGTTCCTGAGGACGTTAACGCCCTATCATCTATTGAGCGGCAAGATAAGCGCTCa > 5:140822/1‑136 (MQ=255) | CGAGAGTTCGCGATGAATGTACAGGCTGCTGTCGTCCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCATTATTCATCTGGCGTTCCTGAGGACGTTAACGCCCTATCATCTATTGAGCGGCAAGATAAGCGCTCA > ACB143/5950001‑5950269 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |