New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	ACB143	= 2172434	113 (1.910)	3 (0.050)	3/238	NT	4.1%	intergenic (+176/+92)	CJ019_02060/CJ019_02061	IS1182 family transposase ISPpu16/hypothetical protein
	?	ACB143	4259053 =	28 (0.480)					coding (402/1254 nt)	nicP_10	Porin‑like protein NicP
Rejected: Frequency below/above cutoff threshold.

AAGAACGACTCACATCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  ACB143/2172301‑2172434 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gCTCGGTCGCGGACATACGCAGCTTCGCCGCCAGCCCGGCCCGACCGTAGTCGTCGGCCGCTCGCCCGTCATCGTGCACCGGCAACAGTTCTGAGCCGCTGGTACCGCCGCCGCTGTCCAGCTTCACGCCGAACAG  >  ACB143/4259053‑4259187                                                                                                                                                                                                                                                                               AAGAACGACTCACATCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGGCT                                                                                                                                       >  3:214639/1‑136                                                                                CCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGGCTCGGTCGCGGACATACGCAGCTTCGCCGCCAGCCCGGCCCGACCGTAGTCGTCGGCCGCTCGCCCGTCATCGTGCACCGG                                                        <  5:369158/136‑1                                                                                         ATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGGCTCGGTCGCGGACATACGCAGCTTCGCCGCCAGCCCGGCCCGACCGTAGTCGTCGGCCGCTCGCCCGTCATCGTGCACCGGCAACAGTTC                                               <  1:165482/136‑1                                                                                                            CATTTCGCGTGTTTTCACACAGCCTGGCTCGGTCGCGGACATACGCAGCTTCGCCGCCAGCCCGGCCCGACCGTAGTCGTCGGCCGCTCGCCCGTCATCGTGCACCGGCAACAGTTCTGAGCCGCTGGTACCGCCG                            <  6:2679/136‑1                                                                                                                                      GCTCGGTCGCGGACATACGCAGCTTCGCCGCCAGCCCGGCCCGACCGTAGTCGTCGGCCGCTCGCCCGTCATCGTTCACCGGCAACAGTTCTGAGCCGCTGGTACCGCCGCCGCTGTCCAGCTTCACGCCGAACAG  >  1:127053/1‑136                                                                                                                                                                                                                                                                               AAGAACGACTCACATCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  ACB143/2172301‑2172434 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gCTCGGTCGCGGACATACGCAGCTTCGCCGCCAGCCCGGCCCGACCGTAGTCGTCGGCCGCTCGCCCGTCATCGTGCACCGGCAACAGTTCTGAGCCGCTGGTACCGCCGCCGCTGTCCAGCTTCACGCCGAACAG  >  ACB143/4259053‑4259187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.