Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	169,705	A→C	20.1%	intergenic (+669/+3018)	PP_RS08750 → / ← fhuA_1	tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	169,705	0	A	C	20.1%	28.0 / 7.6	20	intergenic (+669/+3018)	PP_RS08750/fhuA_1	tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
Reads supporting (aligned to +/- strand):  ref base A (5/11);  new base C (2/2);  total (7/13)
Fisher's exact test for biased strand distribution p-value = 5.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01

AACTTTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTAC  >  ACB143/169660‑169834                                              |                                                                                                                                  aaCTTTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGc                                           >  1:145154/1‑134 (MQ=32) aaCTTTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGc                                           <  2:145154/134‑1 (MQ=32) aaCTTTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCg                                          >  2:261519/1‑135 (MQ=32) aaCTTTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGt                                         <  4:35696/136‑1 (MQ=32) aaCTTTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGt                                         <  6:173264/136‑1 (MQ=32)     ttGTCTGAATATGGGGGGCCCATACTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTTAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAAAAGAACCTGAAACCGTGTCCGTACa                                      >  5:150479/1‑135 (MQ=11)     ttGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTAc                                       >  3:142302/1‑134 (MQ=32)     tagtgtGAATTTGGGGGGACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGATAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACaa                                     >  6:309342/6‑136 (MQ=255)     ctGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTgtacgtac                                       >  6:248678/2‑134 (MQ=255)           gAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTg                               >  6:337245/1‑136 (MQ=255)            aaTATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTgg                              <  2:358729/136‑1 (MQ=255)            aaTAT‑GGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTgg                              <  7:178063/135‑1 (MQ=11)                tGGGGGGACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAg                           <  2:134317/135‑1 (MQ=32)                   ggggACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACAGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTa                       <  1:209021/136‑1 (MQ=17)                           tcctccAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTa               <  5:161022/136‑1 (MQ=32)                           tcctccAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTa               <  4:269401/136‑1 (MQ=32)                                  aGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACt        <  6:171198/136‑1 (MQ=32)                                   ggCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTg       <  5:119250/136‑1 (MQ=32)                                   ggCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTg       <  4:302582/136‑1 (MQ=32)                                        aaTACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTAc  <  6:355561/136‑1 (MQ=32)                                              |                                                                                                                                  AACTTTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTAC  >  ACB143/169660‑169834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑