Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 2,386,955 T→G 26.1% intergenic (+41/+65) CJ019_02252 → / ← dacC_1 hypothetical protein/D‑alanyl‑D‑alanine carboxypeptidase DacC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,386,9550TG26.1% 83.6 / 6.2 77intergenic (+41/+65)CJ019_02252/dacC_1hypothetical protein/D‑alanyl‑D‑alanine carboxypeptidase DacC
Reads supporting (aligned to +/- strand):  ref base T (9/46);  new base G (13/8);  total (23/54)
Fisher's exact test for biased strand distribution p-value = 1.99e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGAAGGCGCCGATGCCTTGCTGTTGCGCGCCGACGCCGGCGTATATGCGGCCAAACAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCGATACAGGTCAAGCAACACCTGATCCAG  >  ACB143/2386821‑2387085
                                                                                                                                       |                                                                                                                                  
cGAAGGCGCCGAGGCCTTGCTGTTGCGCGCCGACGCCGGCGTATATGCGGCCAACCAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTGCGGCCCtt                                                                                                                                   <  8:255245/136‑1 (MQ=255)
  aaGGCGCCGATGCCTTGCTGTTGCGCGCCGACGCCGGCGTATATGCGGCCAAACAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTc                                                                                                                                 <  3:177014/136‑1 (MQ=255)
           aggCCGTGCTGTTGCGCGCCGAGGCCGGCGTATATGCGGCCAAACAGGCCGGGCGGGACCGGGGGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGGCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCaa                                                                                                                        <  5:68437/134‑1 (MQ=255)
           aTGCCTTGGTGTTGCCCGCCGACGCCGGCGTTTATGCGGCCAACCTGGCCGGCCGGGACCGGGGGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGGGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCaa                                                                                                                        <  2:76115/136‑1 (MQ=255)
           aTGCCTTGCTGTTGCGCGCGGACGCCGGCGTATATCCGGCCAAACAGGCCGGGCGGGACCGGGTGGCGGGGGAGCCGGTATAAACCCGTGCGCGCGGTCGGCGGGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCaa                                                                                                                        <  8:197127/136‑1 (MQ=255)
           aTGCCTTGCTGTTGCGCGCCGACGCCGGCGTATATGCGGCCAAACAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCaa                                                                                                                        <  1:273281/136‑1 (MQ=255)
           aTGCCTTGCTGTGGCGCGCCGACGCCGGCGTATATGCGGCCAAACAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCaa                                                                                                                        <  4:138248/136‑1 (MQ=255)
           aTGCCTTGCGGTTGCGCGCCGACGCCGGCGTATATGCGGCCAAACAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCaa                                                                                                                        <  7:60993/136‑1 (MQ=255)
            tGCCTTGCTGTTGCGCGCCGACGCCGGCGTATATGCGGCCAAACAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGGCGCa                                                                                                                         >  6:326738/1‑134 (MQ=255)
            tGCCTTGCTGTTGCGCGCCGACGCCGGCGTATATGCGGCCAAACAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTGCGGCCCGTTGGGGCCGCaaa                                                                                                                       >  1:356781/1‑136 (MQ=255)
            tGCCTTGCTGTTGCGCGCCGACGCCGGCGTATATGCGGCCAAACAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑GTTGCGGCCCGGTCGGGGCGCaa                                                                                                                        >  1:362397/1‑135 (MQ=255)
            tGCCTTGCTGTTGCGCGCCGACGCCGGCGTATATGCGGCCAAACAGGCCGGGCGGGACCGGGGGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCaa                                                                                                                        <  8:80896/135‑1 (MQ=255)
            ggCCTGGCGGTGGCGCCCCGACGCCGACGTATATGCGGCCAAACAGGCCGGGCGGGCCCGGGGGGAGGGGGAGCCGGTATAAAACCGGGCGCGCGGTCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCaa                                                                                                                        <  6:13498/134‑1 (MQ=255)
             gCCGTGCGGTTGCGCGCCTCCGCGGGCGTATAGCCGCCCAACCAGCCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGGGCGCGCGGGCGGCGGGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCAAAg                                                                                                                      <  2:185007/136‑1 (MQ=255)
                    tGTTGCGCGCCGACGCCGGCGTATATGCGGCCAAACAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTCGTGACGCAAAGCCGCTcc                                                                                                               >  4:344483/1‑136 (MQ=255)
                    tGTTGCGCGCCGACGCCGGCGTATATGCGGCCAAACAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGCCGCAAAGCCGCTcc                                                                                                               >  7:122613/1‑136 (MQ=255)
                    tGTTGCGCGCCGACGCCGGCGTATATGCGGCCAAACAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCaa                                                                                                                        <  5:273011/127‑1 (MQ=255)
                    tGTTGCGCGCCGACGCCGGCGTATATGCGGCCAAACAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCaa                                                                                                                        >  6:273011/1‑127 (MQ=255)
                    tGTTGCGCGCCGACGCCGGCGTATATGCGGCCAAACAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑GTTTGGGGCCGTTGGGGGCGCCAAGcccccgc                                                                                                               >  3:52958/1‑131 (MQ=255)
                         cgcgcCGACGCCGGCTTATATGCGGCCAACCCGGGCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAACCCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCAt                                                                                                          <  7:66668/136‑1 (MQ=39)
                                 cGCCGGCGTATATGCGGCCAAACAGGCCGGGCGGGACCGGGGGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACcgcg                                                                                                  <  3:173919/136‑1 (MQ=255)
                                 cGCCGGCGTATATGCGCCCAAACCGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACcgcg                                                                                                  <  1:37259/136‑1 (MQ=255)
                                   ccGGCGTATATGCGGCCAACCCGGCCGGGCGGGACCGGGGGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGGCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCa                                                                                                <  3:210165/136‑1 (MQ=255)
                                   ccGGCGTATATGCGGCCAACCAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCa                                                                                                <  3:242458/136‑1 (MQ=255)
                                   ccGGCGTATATGCGGCCAAACAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGGCGGCGTGGGGGCCGC‑TTTGCGGCCCGTTCGGGACGCAAAGCCGCTCCGGCATCGACCGCGCa                                                                                                <  1:235443/136‑1 (MQ=255)
                                          atatGCGCCCAAACAGGCGGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCggg                                                                                          <  1:370813/135‑1 (MQ=255)
                                           tatGCGGCCAACCCGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGcc                                                                                        <  8:355706/136‑1 (MQ=255)
                                           tatGCGACCAAAACGGCCGGGCGGGCCCGGGTGGCGGGGGAGCCGGTATAAACCAGTGCGCGCGGGCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGcc                                                                                        <  7:74779/136‑1 (MQ=38)
                                             tGCGTCCAACCCGCCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCg                                                                                      <  2:336282/136‑1 (MQ=255)
                                             tGCGCCCAAACTGGCCGGGCGGGCCCGGGGGGAGGGGGAGCCGGTATACGACCGTGCGCGCGGTGGGCGGGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCAAAGCCGCGCCTGCATCGACCGCGCAAGCGGGccc                                                                                       <  1:253266/135‑1 (MQ=38)
                                                   cAAACAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTTCGGCCCGTTGGGGGCGCAAAGCCGCTCCGGGATCGACCCCGCAAACGGGCCCCGgggtg                                                                                >  3:312555/1‑136 (MQ=255)
                                                    aaaCAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGCGTTTGGGCCCGGTTCGGGCCCCAAACCCCCCCCGGCACCGCCCCCCCAcccgggcccggggggg                                                                                >  7:352897/1‑132 (MQ=255)
                                                    aaaCAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTTCGGGCCTTTCGGGGCGCAAAGCCGCGCCGGCATCGCCCGCGCAAGCGGGCCCGGgtgtgt                                                                               >  5:366682/1‑136 (MQ=255)
                                                    aaaCAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGCCGCAAATCCGCTCCTGCATCGACCGCCCAAGCGGGCCCGGgtgtgt                                                                               >  7:317288/1‑136 (MQ=255)
                                                    aaaCAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑GTTTCGGCCCGTTGGGGGCGCCAACCCCCGCCGGCCACCCCCCCGCAACCCGGCCCCGgtgtgt                                                                               >  6:124100/1‑136 (MQ=255)
                                                    aaaCAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑GTTTCGGCCCGGTGGGGGCGCCAACCCGCTCCCGCAAAGCCCCCGCCAGCGGGCCCcggggggt                                                                               >  5:84235/1‑131 (MQ=255)
                                                      acgggcggggcggGACGGGGGGGTGGGGGAGCCGGTATAAAACCGTGCGCGCGGGCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTgg                                                                             <  8:352897/133‑1 (MQ=38)
                                                        aGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTTGGGCCCCTTTGGGCCGCCAAGCCGCGCCGGCCTACGCCCCGCCAGCGGGGCCCGGGGTGTGGcg                                                                           >  7:371149/1‑135 (MQ=255)
                                                           ccGGGCGTGACCGGGGGCAGGGGGAGCCGGTATAAAACCGTGCGCGCGGGCGGCGGGGGGGCCGC‑TTTGCGGCCCGTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGt                                                                        <  6:296775/136‑1 (MQ=255)
                                                                     ccGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGtt                                                              <  1:151695/136‑1 (MQ=255)
                                                                     ccGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGtt                                                              <  1:308013/136‑1 (MQ=255)
                                                                     ccGGGGGGAGGGGGAGCGGGTATAAAACCGTGGGCGCGGTCGGGGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGtt                                                              <  3:213946/136‑1 (MQ=255)
                                                                       gggTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTgg                                                            <  1:147446/136‑1 (MQ=255)
                                                                           ggAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTTCGGCCCGTTGCGGACGCAAACCCCCTCCGGCATCGACCCCGCAAGCGGGCCCCGGTGTGTGGGAGGTGGCGGCAGTTTGCCGc                                                        >  4:373537/1‑136 (MQ=255)
                                                                           ggAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑GTTTCGGGCCGTTAGGGTCCCAAATCCCGGGCGGCAACGACCGCCCGACCGGGCCCCGGTGTGTGGCCGGTGGGCTCAGTTGGCCGc                                                        >  2:326845/1‑136 (MQ=255)
                                                                                    gCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTGCGGCCCGTTCGGGCCGCAAAGCCGCTCCTGCATCGACCCCGCAAGCGGGCCCCGGGGGGTGGCAGGTGGCGTCAGTTGGCCGCTGttcagc                                                >  7:119449/1‑130 (MQ=255)
                                                                                    gCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTGCGGCCCGTTAGGGACGCAAAGCCCCTCCAGCATACCACGCGCAACCGCGCCCGGGTGTGTGGCCCGTGGCGGCCCTTGGCCGCAGTACAgcc                                               >  5:273024/1‑136 (MQ=255)
                                                                                     ccGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTGCGGCCCGTTAGGGGCGCAAAGCCCCGGCTGCAACGACCGCGCAACAGGGCCCGGGGGTGTGGGAGGTGGGGGCATTTTGCCGCTGTACAgcca                                              >  2:338865/1‑136 (MQ=255)
                                                                                            aaacCCGGGCGCGCGGGGGGGGGGGGGGCCGC‑TTTGCGGCCCTTGCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCt                                       <  7:350374/136‑1 (MQ=38)
                                                                                            aaaaCCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCAAAGCCGCGCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGGCAGTTGGCCGCTGTACTGCCAGCCAGCt                                       <  3:198073/136‑1 (MQ=255)
                                                                                            aaaaCCGTGCGCGCGGGCGGCGTGGGGGCCGC‑TTTGCGGCCCGGTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCt                                       <  6:59946/136‑1 (MQ=255)
                                                                                             aaaCCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCt                                       <  8:329964/135‑1 (MQ=255)
                                                                                               aCCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCg                                    <  3:219741/136‑1 (MQ=255)
                                                                                               aCCGGGCGCGCGGGCGGCGTGGGGGCCGC‑TTTGCGGCCCGTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGGCAGTTGGCCGCTGTACTGCCAGCCAGCTTCg                                    <  7:374017/136‑1 (MQ=255)
                                                                                                      gcgcgGTGGGCGGGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGAAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCg                             <  4:74336/136‑1 (MQ=255)
                                                                                                      gcgcgGTCGGCGTGGGGGCCGC‑TTTTCGGCCCTTTCGGGGCGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTGAGTTGGGCGGTGTTCTGCCCGCCAGCTTGGGCTGGCg                             >  8:319438/1‑136 (MQ=255)
                                                                                                      gcgcgGTCGGCGTGGGGGCCGC‑TTTTCGGCCCTTTCGGGCCGCAAAGCCGCGCCTGCCTCGACCGCGCAACCGGGCCCCGGGGTGTGGCAGGGGGCGTCCGGTGGCCGCAGTACTGCCAGCCCGCTTCGGCTGGCg                             >  4:261682/1‑136 (MQ=255)
                                                                                                      gcgcgGTCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCAGCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCg                             <  2:126533/136‑1 (MQ=255)
                                                                                                      gcgcgGTCGGCGTGGGGGCCGC‑TTTGCGGCCCTTGCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCg                             <  8:381399/136‑1 (MQ=255)
                                                                                                      gcgcgGTCGGCGTGGGGGCCGC‑TTTGCGGCCCGTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCg                             <  4:267024/136‑1 (MQ=255)
                                                                                                      gcgcgGTCGGCGTGGGGGCCGC‑GTTGCGGCCCGGTGGGGGCGCAAAGCCGCGCCTGCAACGCCCGCGCCAGCGGGCCCGGGTGTGGTGGAGGTGGCGGGAGGTGGCCGCTGTACAGCCAGCCCGCGTCggcgggcg                             >  2:230494/1‑136 (MQ=255)
                                                                                                      gcgcgGTCGGCGGGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCg                             <  3:153093/136‑1 (MQ=255)
                                                                                                      gcgcgGGGGGGGTGGGGGCGGC‑TTTGCGGCCCTTTCGGGACGCAAAGCCGGCCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGATGTACTGCCAGCCAGCTTCGGCTGGCg                             <  4:7383/136‑1 (MQ=255)
                                                                                                      gcgcgGGCGGCGGGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGACGCTGTACTGCCAGCCAGCTTCGGCTGGCg                             <  7:65208/136‑1 (MQ=255)
                                                                                                        gcgGTCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCCGCTTCGGCTGGCGa                            >  3:277094/1‑135 (MQ=255)
                                                                                                                cGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCGATACAGGTCa                   <  7:368710/136‑1 (MQ=255)
                                                                                                                 ggggtggCCGC‑TTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCAGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCGATACAGGTCaa                  <  1:318169/131‑1 (MQ=255)
                                                                                                                 gggggggCCGC‑TTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCGATACAGGTCaa                  <  2:274388/134‑1 (MQ=255)
                                                                                                                      ggCCGC‑TTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCGATACAGGTCAAGCAac             <  3:374533/136‑1 (MQ=255)
                                                                                                                      ggCCGC‑TTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCGATACAGGTCAAGCAac             <  2:54077/136‑1 (MQ=255)
                                                                                                                         cGC‑TTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCGGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGGCAGTGGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCGATACAGGTCAAGCAACAcc          <  2:362397/136‑1 (MQ=255)
                                                                                                                         cGC‑TTTGCGGCCCGTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCGATACAGGTCAAGCAACAcc          <  2:356781/136‑1 (MQ=255)
                                                                                                                           c‑TTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCGATACAGGTCAAGCAACACCTg        <  1:95258/136‑1 (MQ=255)
                                                                                                                               tGCGGCCCTTGCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGGGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCGATACAGGTCAAGCAACACCTGa       <  1:305681/134‑1 (MQ=255)
                                                                                                                                gCGGCCCGTTGGGGCCGCAAAGCCGCGCCTGCATCGACCGCGCATGCGGGCCCGGGGGTGTGGCAGGGGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCGATACAGGTCAAGCAACACCTGATcc    <  1:302483/136‑1 (MQ=255)
                                                                                                                                gCGGCCCGTTCGGCACGCAAAGCCGCTCCTGCAGCGACCGCGCAAGCGGGCCCGGGGGTGTGGCAGGTGGCGGCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCGATACAGGTCAAGCAACACCTGATcc    <  6:235310/136‑1 (MQ=255)
                                                                                                                                 cGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGGGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCGATACAGGTCAAGCAACACCTGATcc    <  1:288771/135‑1 (MQ=255)
                                                                                                                                  ggCCCTGTCGGCCCGCAACGCCGCTCCGGCAGCTCCCGCGCAAGCGGGCCCGGGTGGGGGCCAGGGGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCGATACAGGTCAAGCAACACCTGATCCAg  <  6:211976/136‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                  
CGAAGGCGCCGATGCCTTGCTGTTGCGCGCCGACGCCGGCGTATATGCGGCCAAACAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGC‑TTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCGATACAGGTCAAGCAACACCTGATCCAG  >  ACB143/2386821‑2387085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑