Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,805,499 C→A 6.7% intergenic (+426/‑323) dsbG → / → CJ019_04513 Thiol:disulfide interchange protein DsbG/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,805,4990CA 6.7% 471.0 / 2.3 152intergenic (+426/‑323)dsbG/CJ019_04513Thiol:disulfide interchange protein DsbG/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (117/22);  new base A (2/11);  total (119/33)
Fisher's exact test for biased strand distribution p-value = 6.40e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 153 total aligned reads displayed.

TTGGACGCAGGCCGTTCTTTCGCAAGCAGTAAATCTCCCGAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAGAAGCTTCTTACATAAATATAAGAAGCACTGAAAACT  >  ACB143/4805365‑4805633
                                                                                                                                       |                                                                                                                                      
ttGGACCCAGGCCGTTCTTTCGCAACCAGAAAATCTCCCGAAAACAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCa                                                                                                                                       <  6:240754/136‑1 (MQ=255)
  ggACGCAGGCCGTTCTTTCGCAAGCAGTAAATCTCCCGAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCaaa                                                                                                                                     >  5:254956/1‑136 (MQ=255)
  ggACGCAGGCCGTTCTTTCGCAAGCAGTAAATCTCCCGAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCaa                                                                                                                                      <  6:229266/135‑1 (MQ=255)
    aCGCAGGCCGTTCTTTCGCAAGCAGTAAATCTCCCGAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACa                                                                                                                                   >  6:150382/1‑136 (MQ=255)
     cGCAGGCCGTTCTTTCGCAAGCAGTAAATCTCCCGAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACAt                                                                                                                                  >  4:96315/1‑136 (MQ=255)
     cGCAGGCCGTTCTTTCGCAAGCAGTAAATCTCCCGAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACAt                                                                                                                                  >  4:362000/1‑136 (MQ=255)
     cGCAGGCCGTTCTTTCGCAAGCAGTAAATCTCCCGAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACAt                                                                                                                                  >  4:176651/1‑136 (MQ=255)
     cGCAGGCCGTTCTTTCGCAAGCAGTAAATCTCCCGAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAAACCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACAt                                                                                                                                  >  3:274743/1‑136 (MQ=255)
       cAGGCCGTTCTTTCGCAAGCAGTAAATCTCCCGAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACAt                                                                                                                                  >  7:316592/1‑134 (MQ=255)
       cAGGCCGTTCTTTCGCAAGCAGTAAATCTCCCGAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACAt                                                                                                                                  >  2:186448/1‑134 (MQ=255)
                 tttCGCAAGCAGTAAATCTCCCGAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTcgc                                                                                                                      >  1:271586/1‑136 (MQ=255)
                 tttCGCAAGCAGTAAATCTCCCGAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTATAACCATGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAAAATCAGCATGCTccc                                                                                                                      >  8:281869/1‑134 (MQ=255)
                     gCAAGCAGTAAATCTCCCGAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGccc                                                                                                                  >  8:121442/1‑136 (MQ=255)
                     gCAAGCAGTAAATCTCCCGAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGccc                                                                                                                  >  2:353640/1‑136 (MQ=255)
                      cAAGCAGTAAATCTCCCGAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGcccc                                                                                                                 >  8:127326/1‑136 (MQ=255)
                       aaGCAGTAAATCTCCCGAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATATAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCt                                                                                                                >  4:221586/1‑136 (MQ=255)
                       aaGCAGTAAATCTCCCGAAATCAATCTCACGAACCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATATATAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCt                                                                                                                >  2:164219/1‑136 (MQ=255)
                                aTCTCCCGAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAAc                                                                                                       >  3:55044/1‑136 (MQ=255)
                                aTCTCCCGAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAAc                                                                                                       >  2:323236/1‑136 (MQ=255)
                                  ctcCCGAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACtt                                                                                                     >  3:302269/1‑136 (MQ=255)
                                   cccccAAATAAATCACCCGCCCCGCAAAATAAACCCCCCCGCTGTTCAAACCGTGAAACTAAAAATATATATAAACATTTCTTCGGAGATTGGTTATTTAAAACATCAGCTTGGTCGCGCCCCTTATTAAAACTTc                                                                                                    <  6:345122/135‑1 (MQ=255)
                                      cGAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAACCATCAGCATGCTCGCGCCCCTTATTAAAACTTcaca                                                                                                 >  5:118293/1‑136 (MQ=255)
                                      cGAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTcaca                                                                                                 >  8:276752/1‑136 (MQ=255)
                                       gAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCacac                                                                                                >  1:300052/1‑136 (MQ=255)
                                       gAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCacac                                                                                                >  3:362500/1‑136 (MQ=255)
                                        aaaTCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACAcc                                                                                               >  2:295697/1‑136 (MQ=255)
                                        aaaTCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACAcc                                                                                               >  6:182271/1‑136 (MQ=255)
                                        aaaTCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACAcc                                                                                               >  1:389936/1‑136 (MQ=255)
                                          atcaatcTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACcgc                                                                                             >  2:214024/1‑136 (MQ=255)
                                          atcaatcTCACGACCCGGCAAATCAATCCCCACCCAGGTCAACCCCTCCAACTCAAAATATCTAGAAACAATTCTACGGAGCATGCAGATGTCAAACATCAGCATGCTCGCGCCCCTAATAAAAAATACACACcgc                                                                                             >  4:285389/1‑136 (MQ=255)
                                              atcTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCa                                                                                          >  1:76238/1‑135 (MQ=255)
                                                  cACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAAc                                                                                      >  2:352956/1‑135 (MQ=255)
                                                   accaCCCGGAAAATCTATCCCCACGCAGGTAAAACCCTGAAAATAAAAATATATATAAAAAATTCTTCGGACCATGCTGTTTTCAAAAATCAGATTGCGCGCGCCCCTTATTAAAACTTCACACCGCGCATAAc                                                                                      <  2:307907/131‑1 (MQ=255)
                                                             aaaTCTAACCCCACGCAGGTCAAACCCTGAAACAAAAAATATCTACAAACAATTCTTCGGAGCATGCTGATGACAAACATCAGCAGGCCCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTca                                                                          <  8:202280/136‑1 (MQ=255)
                                                             aaaTCTAACCCCACCCAGGTAAACCCCTGAAACAAAAAATATATCAAAAAAATTCTTCGCACAAGGCTGATGTAAAAAATCAGAAGGCCCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTca                                                                          <  6:124420/136‑1 (MQ=255)
                                                                acTACCCCCCCGCAGGTCAAACCCTGAAACTCAAAATATATACAAAAAATTATTCGGAGCATGCTGATGTAAAAAATCAGCATGCACGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCacac                                                                       <  6:80154/135‑1 (MQ=255)
                                                                    tCCCCACGCAGTTATAACCCTGAAACTAAAAATATCTACAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCGCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCacac                                                                       <  8:316592/132‑1 (MQ=255)
                                                                       ccACGCAGTTCTAACCATTAAACTAAAAAAATCTAGAAACAATTCTTGGTCCATGGGTGATGTCAAACATAAGATTGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTcacc                                                                <  8:89914/136‑1 (MQ=255)
                                                                       ccACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCCCACGCTcac                                                                 >  5:324051/1‑135 (MQ=255)
                                                                       ccACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTcac                                                                 >  3:393173/1‑135 (MQ=255)
                                                                       ccACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTcac                                                                 >  5:179019/1‑135 (MQ=255)
                                                                       ccACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGACCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTcac                                                                 >  6:11241/1‑135 (MQ=255)
                                                                        cACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTcacca                                                               >  7:145900/1‑136 (MQ=255)
                                                                        cACGCAGGTCAAACCCTGAAACTAAAAATATCTACAAACAATTCTTCGGAGAATGCTGATGTAAAACATCAGCTTGCCCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTcacca                                                               <  3:257059/136‑1 (MQ=255)
                                                                            cAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACcaca                                                             >  7:118115/1‑134 (MQ=255)
                                                                             aGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACagag                                                          >  1:1123/1‑136 (MQ=255)
                                                                             aGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACagag                                                          >  6:290202/1‑136 (MQ=255)
                                                                             aGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACagag                                                          >  6:276177/1‑136 (MQ=255)
                                                                             aGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACagag                                                          >  2:91357/1‑136 (MQ=255)
                                                                             aGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACagag                                                          >  4:76392/1‑136 (MQ=255)
                                                                                tcaaaCCCTGCAAATAAAAATATCTACAACCAATTCTTCGTCGCTTGCTGAGGCCAAACATCCGCAGGCGCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCtt                                                       <  2:251729/133‑1 (MQ=255)
                                                                                  tAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCCCCACAGAGCTTTc                                                     >  6:382939/1‑136 (MQ=255)
                                                                                  tAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTc                                                     >  8:131505/1‑136 (MQ=255)
                                                                                     cccTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTAACACCGCGCATACCAAAGAACACTCCCACGCTCACCACAGAGCTTTCGCa                                                  >  5:291617/1‑136 (MQ=255)
                                                                                       cTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACg                                                >  4:195857/1‑136 (MQ=255)
                                                                                       cTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACg                                                >  3:10652/1‑136 (MQ=255)
                                                                                       cTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACg                                                >  2:391732/1‑136 (MQ=255)
                                                                                       cTGAAACTAAAAATATCTACAAACAATTCTTCGGAGCATGCTGATTTCAAAAATAAGAATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAATAACACTCACACTCTCACCACAGAGCTTTCGCagt                                                <  8:220946/136‑3 (MQ=255)
                                                                                       cTGAAACTAAAAATATCAACAAACAATTCTTCGGACCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACg                                                <  7:84669/136‑1 (MQ=255)
                                                                                        tGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGc                                               >  1:158155/1‑136 (MQ=255)
                                                                                            aCTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAACCTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACCGAGCTTTCTCACGCCAg                                            >  1:301658/1‑135 (MQ=255)
                                                                                            aCTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCCCCACAGAGCTTTCGCACGCCAg                                            >  4:354574/1‑135 (MQ=255)
                                                                                            aCTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCat                                            >  6:159311/1‑134 (MQ=255)
                                                                                            aCTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAg                                            >  8:51958/1‑135 (MQ=255)
                                                                                            aCTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAg                                            >  2:167114/1‑135 (MQ=255)
                                                                                            aCTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAg                                            >  2:11225/1‑135 (MQ=255)
                                                                                            aCTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAg                                            >  7:112828/1‑135 (MQ=255)
                                                                                            aCTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAg                                            >  1:297469/1‑135 (MQ=255)
                                                                                            aCTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAg                                            >  7:143392/1‑135 (MQ=255)
                                                                                            aCTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAg                                            >  7:242890/1‑135 (MQ=255)
                                                                                            aCTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAg                                            >  1:52866/1‑135 (MQ=255)
                                                                                            aCTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAg                                            >  8:187986/1‑135 (MQ=255)
                                                                                            aCTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAg                                            >  3:271582/1‑135 (MQ=255)
                                                                                            aCTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAg                                            >  6:236748/1‑135 (MQ=255)
                                                                                            aCTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAg                                            >  3:12868/1‑135 (MQ=255)
                                                                                            aCTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAg                                            >  5:164315/1‑135 (MQ=255)
                                                                                            aCTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAg                                            >  5:391277/1‑135 (MQ=255)
                                                                                            aCTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAg                                            >  2:399091/1‑135 (MQ=255)
                                                                                            aCTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCACTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAg                                            >  7:52932/1‑135 (MQ=255)
                                                                                            aCTCAAAATATCAAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAg                                            >  1:390383/1‑135 (MQ=255)
                                                                                             ctaaaaaTATCTAGAAACAATTCTTCGGATCATGCTGATGTAAAACATCAGCATGCTCTACCCCCTTAAAAAAACTTCACACATCGCAAAACAAATAACACTCACACTCTCACCACAGAGCTTTCACACGCCAGa                                           >  2:59972/4‑135 (MQ=255)
                                                                                             cTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGaa                                          >  2:321690/1‑136 (MQ=255)
                                                                                             cTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGaa                                          >  3:118289/1‑136 (MQ=255)
                                                                                             cTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGaa                                          >  5:283806/1‑136 (MQ=255)
                                                                                              taaaaaTATCTACAAAAATTTCTTCGGCGCATGCTGATGTAAAACATCAGAATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAAt                                         <  1:323236/134‑1 (MQ=255)
                                                                                                  aaTATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAg                                      >  5:228859/1‑135 (MQ=255)
                                                                                                   atatCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAg                                      >  2:132609/1‑134 (MQ=255)
                                                                                                     atCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAGAAGc                                  >  6:57144/1‑136 (MQ=255)
                                                                                                      tcaacaaaaaaTTGTTCGCAGCAGGCTGATTTCAAAAAACGGAATGCGCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAGAAGct                                 <  6:366921/131‑1 (MQ=255)
                                                                                                          taaaCAATTTTGCGCAGCATGTTTAGGACAAACATCATGTTGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAGAAGcttctt                             <  4:22411/135‑1 (MQ=255)
                                                                                                          gAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACAGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAGAAGcttct                              >  7:39407/1‑135 (MQ=255)
                                                                                                          aaaaCAATTCTTCGTAGCATGCTTATGACAAAAATAAGATTGCCCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAGAAGcttctt                             <  1:266498/135‑1 (MQ=255)
                                                                                                          aaaaCAATTCTGCGTCGAATGGTCAGTTAAAACATAAGCTTGCACGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAGAAGcttctt                             <  6:390528/135‑1 (MQ=255)
                                                                                                             aaaaTTCTTCGCACCATCCTTATGAAAACCATCAGATTGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAGAAGCTTCTTACa                          <  5:267552/134‑1 (MQ=255)
                                                                                                             aCAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAGAAGCTTCTTACa                          >  4:25652/1‑136 (MQ=255)
                                                                                                                   cttcGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAGAAGCTTCTTACATAAata                    >  8:23062/1‑136 (MQ=255)
                                                                                                                            aTGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAGAAGCTTCTTACATAAATATAAGAAGCa           >  7:200770/1‑136 (MQ=255)
                                                                                                                            aTGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAGAAGCTTCTTACATAAATATAAGAAGCa           >  5:378635/1‑136 (MQ=255)
                                                                                                                                  gATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATAAGAAGCTTCTTACATAAATATAAGAAGAACTGa       >  3:197725/1‑135 (MQ=255)
                                                                                                                                      tCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAGAAGCTTCTTACATAAATATAAGAAGCACTGAAAAc   <  6:179019/135‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
TTGGACGCAGGCCGTTCTTTCGCAAGCAGTAAATCTCCCGAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAGAAGCTTCTTACATAAATATAAGAAGCACTGAAAACT  >  ACB143/4805365‑4805633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: