Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB143352,7270AC35.4% 49.2 / 11.7 48intergenic (+37/+83)cdhR_1/CJ019_00326HTH‑type transcriptional regulator CdhR/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (9/22);  new base C (12/5);  total (21/27)
Fisher's exact test for biased strand distribution p-value = 7.44e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGCCAGCGGTGATCTGTGGGGGCGGGCTTGTTCCGCGAAGGGGCCGGTCCTGGGTAAATGGATTACGCGCGTTTGTTGAACTGCGCCAACGCTGGCAACAGT  >  ACB143/352593‑352849
                                                                                                                                      |                                                                                                                          
cGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCcaca                                                                                                                          <  6:451131/137‑1 (MQ=255)
      cGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCAc                                                                                                                    <  6:404937/137‑1 (MQ=255)
        cAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCAccc                                                                                                                  <  2:166984/137‑1 (MQ=255)
           gCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCCCGCCCCCCCGTCAcccccc                                                                                                               >  7:27577/1‑134 (MQ=255)
                   ccTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGa                                                                                                       >  7:414894/1‑137 (MQ=255)
                   ccTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCCCTCCCCCACGTCACCCCCTGCGCTTgc                                                                                                       >  2:394156/1‑136 (MQ=255)
                    cTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAg                                                                                                      <  1:387487/137‑1 (MQ=255)
                     tGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGc                                                                                                     <  8:414894/137‑1 (MQ=255)
                       gcgccgcACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGCCa                                                                                                   <  2:37548/136‑1 (MQ=255)
                        cgccgcACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGGCCCCCCCCCCAGGGCAACCCCCGGGCGAGAGCCac                                                                                                  >  1:374483/1‑136 (MQ=255)
                        cgccgcACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCCCTCCCACAGGTCACCCGCTGAGCGTTCGCCAg                                                                                                  >  4:116068/1‑137 (MQ=255)
                          ccgcACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGCCAGCg                                                                                                <  4:211640/137‑1 (MQ=255)
                          ccgcACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCCCGCCCCCCCGGCACCCGCCGGGCGTGGGCCcgcg                                                                                                >  3:313078/1‑137 (MQ=255)
                          ccgcACAGCGGGCCGATGGCGGCCCGGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGCCAGCg                                                                                                <  8:2066/137‑1 (MQ=255)
                            gcACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGCCAGCGGt                                                                                              <  6:68555/137‑1 (MQ=255)
                              acaGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGCCa                                                                                                   <  3:464148/130‑1 (MQ=255)
                              acaGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGCCa                                                                                                   >  4:464148/1‑130 (MQ=255)
                                aGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCCCACGTCACCCGCTGAGCGTGAGCCAGCGGTgctg                                                                                          >  7:475360/1‑134 (MQ=255)
                                aGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGCCAGCGGTGATc                                                                                          <  3:247749/137‑1 (MQ=255)
                                     gCCGCTGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGCCAGCGGTGATCTGTgg                                                                                     <  5:451866/137‑1 (MQ=255)
                                     gCCGCTGGCGGCCCGGCGCCAGGCGCGCAATGAGTCGACATTTGCCCGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGGCACCCGCTGAGCTTGAGCCAGCGGTGATCTGTgg                                                                                     <  2:182119/137‑1 (MQ=39)
                                     gCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACCCCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACCGGTCACCCGCTGAGCTTGAGCCAGCGGTGATCTGTgg                                                                                     <  3:150240/137‑1 (MQ=255)
                                          tGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCGCCCCCACGGGCCCCCCGGAGCGTGTCCCCGCGGTGAGATGgggggggg                                                                                >  8:293829/1‑135 (MQ=255)
                                          tGGCGCCCCAGAGCACGCCCCGAATTGAGTCGTCATATTCCAGTGTTAGCCTCTGACACCCCCCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTCTCCTTGAGCCAGCGGTGATGTGTGGgggcg                                                                                <  4:431718/137‑1 (MQ=19)
                                             cggcCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCGGAGCTTGAGCCCGCGGGGGTCTGTGGgggcgggg                                                                             >  8:284442/1‑136 (MQ=255)
                                                  cTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCCCACGTGACCCCCGGAGCTTGAGCCCCCGGGGATGTGGGGGGGCGGGCTTGtt                                                                        >  4:30891/1‑137 (MQ=255)
                                                   tGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCCCTCCCCCAGGTCACCCGCTGAGCGTGAGCCAGCGGTGATCTGGGGGGGCGGGCGTGttg                                                                       >  8:91024/1‑136 (MQ=255)
                                                           gcgcgcAATGAGTCGACATTTGCCCGTGTACGGCGCTGCCCCCGCGCCGGCCTGTGCGCGGGCACGCCCGCTCCCACCGGTCCCCCGCTGAGCTGCCGCCAGCGGTGATCTGGGGGGGCGGGCTTGTTCCGCGAAgg                                                               <  6:112846/137‑1 (MQ=255)
                                                             gcgcAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACACGGCACCCGCTGAGCTTGAGCCAGCGGTGATCTGTGGGGGCGGGGTTGTTACGCGAAgggg                                                             >  4:68976/1‑137 (MQ=255)
                                                             gcgcAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCCCCCCCCCCCGGCACCCCCAGAGCGTTAGCCAGCGGGGGACAGTGGGGGCGGGGGTGTTTCGCCGAgggg                                                             >  4:22540/1‑137 (MQ=255)
                                                                            aTTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCCCTCCCACAGGTAAACCGCTGAGCGTGAGCCAGCGGTGATGTGTGGGGGCGGGCGTGTTACGCGAAGGGGCCGGTGCTGGGTaaa                                              >  7:370819/1‑137 (MQ=38)
                                                                                  cAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTg                                                                                                        <  5:444492/73‑1 (MQ=255)
                                                                                  cAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTg                                                                                                        >  6:444492/1‑73 (MQ=255)
                                                                                  cAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGCCa                                                                                                   >  3:32803/1‑78 (MQ=255)
                                                                                  cAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGCCa                                                                                                   <  4:32803/78‑1 (MQ=255)
                                                                                     tgtAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGCCAGGGGTGATCTGTGGGGGCGGGCTTGTTCCGCGAAGGGGCCGGTCCTGGGTAAATGGATTAcg                                     <  7:284442/137‑1 (MQ=255)
                                                                                     ggtACGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGCGCTTGAGCCAGCGGTGATCTGTGGGGGCGGGCTTGTTCCGCGAAGGGGCCGGTCCTGGGTAAATGGATTAcg                                     <  7:253912/136‑1 (MQ=255)
                                                                                        aaGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCCCAGGTCACCCGCTGAGCTTGAGCCAGCGGTGATCTGTgg                                                                                     >  8:241559/1‑86 (MQ=255)
                                                                                        aaGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGCCAGCGGTGATCTGTgg                                                                                     <  7:241559/86‑1 (MQ=255)
                                                                                        aaGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCCCACCCCCACGTCCACCCCGGAGCGTGGTCCCCCCGGGAACGGTGGGGGGGGGCGTGTTTCCAGAAGGGGCCCGGCCGGGGGAAAAGGATTAagcgc                                  >  8:249740/1‑137 (MQ=255)
                                                                                                gACCCCGCGCGGGCCTCTTCGCGGCCACGCCCGCTCCCCCAGGCCACCCGCTGAGCTTGAGCCAGCGGTTATGTGTGGGGGCGGGCTTGTTCCGCGAAGGGGCCGGTCCTGGGTAAATGGATTACGCGCGTttgttg                          <  3:68976/137‑1 (MQ=255)
                                                                                                ccccccGCGCCGGCCGCTTCGCGGGCACGCCCGCTCCCACAGGGCACCCGCGTAGCTTGAGCCCGGGGTGATCTGGGGGGGCGGGCTTGTTCCGCGAAGGGGCCGGTCCTGGGTAAATGGATTACGCGCGTttgttg                          <  7:435596/133‑1 (MQ=255)
                                                                                                  cacCGCGCCGGCCTCTTCGCGGGCACGCCCGCGCCCACAGGTCACCCACTGGCCTTGCGCCAGCGGTGATGTGTGGGGGCGGGGGTGTTCAGCGAAGGGGGCGGGGCGGGGTAAATGGTATACTCGCGTGTGGTGaa                        >  5:405079/1‑137 (MQ=19)
                                                                                                          cGGCCTCTTCGCGGGCACGCCCCCTCCCCCCTGTCACCCGCTGCGCTTGAGCCCGCGGGGATGTGTGGGGGCGGGCTTGTTCCGCGAAGGGGCCGGGCCTGGGTTAAAGGATTAAGCGGGGTTGTTGAACAGCGGCa                >  6:342053/1‑137 (MQ=255)
                                                                                                          cGGCCTCTTCCCGGGCACCCCCGCCCCCCCAGGACACCCGCTCAGCTTGCGCCAGCGGTGATCTGGGGGGGCGGGCTTGTTCCGCGAAGGGGCCGGTCCTGGGTAAATGGATTACGCGCGTTTGTTGAACTGCGCCa                <  7:212661/137‑1 (MQ=255)
                                                                                                            gCCTCTTCGCGGGCACGCCCGCTCCCCCAGGTCACCCGCTGAGCTGGCGCCAGCGGTGATCTGTGGGGGCGGGCTGGTTCCGCGAAGGGGCCGGTCCTGGGTAAATGGATTACGCGCGTTTGTTGAACTGCGCCAAc              <  8:475360/137‑1 (MQ=255)
                                                                                                            gCCTCTGCGCGGCCCCGCCCGCCCCCACAGGTCCCCCGCTGCGCTTGCGCCCGCGGTGATCGGTGGGGGCGGGCTTGTTCCGCGAAGGGGCCGGTCCTGGGTAAATGGATTACGCGCGTTTGTTGAACTGCGCCAAc              <  1:404598/137‑1 (MQ=255)
                                                                                                                    gcgGGCCCCCCCCCCCCCCCTGGGCCCCGGCTGCGGTTGCGCCAGGGGTGTGGTGTGGGGGGGGGGTTGGTCCGCGACGGGGCCGGTCCTGGGTAAATGGATTACGCGCGTTTGTTGAACTGCGCCAACGCTGGCaa      <  6:305850/137‑1 (MQ=255)
                                                                                                                        ccccGCCCGCGCCCCCCGGTCCCCCGGTTCGCTGGCCCCTGGGGTGTTGTGTGGGGGGGGGTTTGTTCCGCGATGGGGCCGGTCCTGGGTAAATGGATTACGCGCGTTTGTTGAACTGCGCCAACGCTGGCAACAGt  <  3:30891/134‑1 (MQ=255)
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CGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGCCAGCGGTGATCTGTGGGGGCGGGCTTGTTCCGCGAAGGGGCCGGTCCTGGGTAAATGGATTACGCGCGTTTGTTGAACTGCGCCAACGCTGGCAACAGT  >  ACB143/352593‑352849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑