Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 1,754,756 A→G 29.3% K287R (AAG→AGG)  CJ019_01665 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1431,754,7560AG29.3% 81.7 / 47.7 58K287R (AAG→AGG) CJ019_01665hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (22/19);  new base G (10/7);  total (32/26)
Fisher's exact test for biased strand distribution p-value = 7.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01

GCGCGATCTACGGCTTGAAGTACAAGGAAGAAGAGCAACTGCTCGCTGGCGACGGCACCGGCCAGAACCTGCTGGGCCTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAAGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCCATTGCCCTGAACCCGGTCGACTGGGCGGACATCGAGCTGACCAAGGACGCCAACGGCTCCTACATCTGGGTGA  >  ACB143/1754624‑1754890
                                                                                                                                    |                                                                                                                                      
gcgcgATCTACGGCTTGAAGTACAAGGAAGAAGAGCAACTGCTCGCTGGCGACGGCACCGGCCAGAACCTGCTGGGCCTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAAGa                                                                                                                                      <  2:303346/135‑1 (MQ=255)
  gcgATCTACGGCTTGAAGTACAAGGAAGAAGAGCAACTGCTCGCTGGCGACGGCACCGGCCAGAACCTGCTGGGCCTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAGGATc                                                                                                                                    >  2:262454/1‑135 (MQ=255)
  gcgATCTACGGCTTGAAGTACAAGGAAGAAGAGCAACTGCTCGCTGGCGACGGCACCGGCCAGAACCTGCTGGGCCTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAAGATc                                                                                                                                    >  6:84462/1‑135 (MQ=255)
             cTTGAAGTACAAGGAAGAAGAGCAACTGCTCGCTGGCGACGGCACCGGCCAGAACCTGCTGGGCCTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAAGATCGATACTCTgcgc                                                                                                                        >  6:81506/1‑136 (MQ=255)
                 aaGTACAAGGAAGAAGAGCAACTGCTCGCTGGCGACGGCACCGGCCAGAACCTGCTGGGCCTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAAGATCGATACTCTGCGCcgcg                                                                                                                    <  6:122058/136‑1 (MQ=255)
                 aaGTACAAGGAAGAAGAGCAACTGCTCGCTGGCGACGGCACCGGCCAGAACCTGCTGGGCCTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAAGATCGATACTCTGCGCcgcg                                                                                                                    <  3:213441/136‑1 (MQ=255)
                  aGTACAAGGAAGAAGAGCAACTGCTCGCTGGCGACGGCACCGGCCAGAACCTGCTGGGCCTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAGGATCGATACTCTGCGCcgcg                                                                                                                    <  1:298634/135‑1 (MQ=255)
                   gTACAAGGAAGAAGAGCAACTGCTCGCTGGCGACGGCACCGGCCAGAACCTGCTGGGCCTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAAGATCGATACTCTGCGCcgcgc                                                                                                                   >  6:5655/1‑135 (MQ=255)
                   gTACAAGGAAGAAGAGCAACTGCTCGCTGGCGACGGCACCGGCCAGAACCTGCTGGGCCTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAAGATCGATACTCTGCGCcgcgc                                                                                                                   >  5:84973/1‑135 (MQ=255)
                   gTACAAGGAAGAAGAGCAACTGCTCGCTGGCGACGGCACCGGCCAGAACCTGCTGGGCCTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAAGATCGATACTCTGCGCcgcgc                                                                                                                   >  4:136274/1‑135 (MQ=255)
                         ggAAGAAGAGCAACTGCTCGCTGGCGACGGCACCGGCCAGAACCTGCTGGGCCTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAAGATCGATACTCTGCGCCGCGCGATCCTg                                                                                                            >  6:198287/1‑136 (MQ=255)
                          gaagaaGAGCAACTGCTCGCTGGCGACGGCACCGGCCAGAACCTGCTGGGCCTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAGGATCGATACTCTGCGCCGCGCGATCCTg                                                                                                            >  8:190488/1‑135 (MQ=255)
                               agagCAACTGCTCGCTGGCGACGGCACCGGCCAGACCCTGCTGGGCCTGATTCCTCAGGCCACCGCCGTCAACAACGCCCTGCGCAAAGCCGGCGACACCAAGATCGATACTCTGCGCCGCGCGATCCTGCAGGTc                                                                                                      >  2:246486/1‑136 (MQ=255)
                               agagCAACTGCTCGCTGGCGACGGCACCGGCCAGAACCTGCTGGGCCTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAAGATCGATACTCTGCGCCGCGCGATCCTGCAGGTc                                                                                                      >  8:93569/1‑136 (MQ=255)
                               agagCAACTGCTCGCTGGCGACGGCACCGGCCAGAACCTGCTGGGCCTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAAGATCGATACTCTGCGCCGCGCGATCCTGCAGGTc                                                                                                      >  4:115293/1‑136 (MQ=255)
                                   cAACTGCTCGCTGGCGACGGCACCGGCCAGAACCTGCTGGGCCTGAGTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAAGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCa                                                                                                  <  6:153879/136‑1 (MQ=255)
                                     aCTGCTCGCTGGCGACGGCACCGGCCAGAACCTGCTGGGCCTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAAGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATc                                                                                                <  5:198287/136‑1 (MQ=255)
                                     aCTGCGCGCTGGCGACGGCACCGGCCAGAACCTGCTGGGCCTGATTCCGCAGGCCACCGCCTTCAACAACGCCCTGCGCACAGCCGGCGACACCAAGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATc                                                                                                <  8:83648/136‑1 (MQ=255)
                                            gCTGGCGACGGCACCGGCCAGAACCTGCTGGGCCTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAAGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAg                                                                                          >  2:115923/1‑135 (MQ=255)
                                                 cGACGGCACCGGCCAGAACCTGCTGGGCCTGATTCCTCAGGCCACCGCCTTCACCCACGCCCTGCGCAAAGCCGGCGACACCAAGATCGATACTCTGCGCCGCGCGATCCTGCAGGTGCGCATCGCCGAGTACcgc                                                                                    <  4:195155/136‑1 (MQ=255)
                                                   aCGGCACCGGCCAGAACCTGCTGGGCCTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAAGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCgcgc                                                                                  <  4:291545/136‑1 (MQ=255)
                                                    cGGCACCGGCCAGAACCTGCTGGGCCTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAAGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGcc                                                                                 >  1:210554/1‑136 (MQ=255)
                                                      gCACCGGCCAGAACCTGCTGGGCCTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAGGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTc                                                                               >  1:115476/1‑136 (MQ=255)
                                                        aCCGGCCAGAACCTGCTGGGCCTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAGGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCgg                                                                             <  1:253480/136‑1 (MQ=255)
                                                        aCCGGCCAGAACCTGCTGGGCCTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAAGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCgg                                                                             <  1:200938/136‑1 (MQ=255)
                                                        aCCGGCCAGAACCTGCTGGGCCTGATTCCTCAGGCCACCGCCTTCAACAACGACCTGCGCAAAGCGGGCGACACCAAGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCgg                                                                             <  4:104867/136‑1 (MQ=255)
                                                               aGAACCTGCTGGGCCTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAGGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCCATTGc                                                                      >  8:54624/1‑136 (MQ=255)
                                                               aGAACCTGCTGGGCCTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAAGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCCATTGc                                                                      >  3:125380/1‑136 (MQ=255)
                                                                   ccTGCTGGGCCTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAAGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCCATTGCCCTg                                                                  <  2:69115/136‑1 (MQ=255)
                                                                         gggCCTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAGGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCCATTGCCCTGAACCCg                                                            >  1:137282/1‑136 (MQ=255)
                                                                         gggCCTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAAGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCCATTGCCCTGCACCCg                                                            >  2:104701/1‑136 (MQ=255)
                                                                         gggCCTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAAGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCCATTGCCCTGAACCCg                                                            >  5:70293/1‑136 (MQ=255)
                                                                         gggCCTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAAGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCCATTGCCCTGAACCCg                                                            >  1:18763/1‑136 (MQ=255)
                                                                         gggCCTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAAGATCGATACTCTGCGCCGCGCGATCATGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGACATTGCCCTGAACCCg                                                            >  5:70295/1‑136 (MQ=255)
                                                                          ggCCTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAGGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCCATTGCCCTGAAccc                                                             <  3:65443/134‑1 (MQ=255)
                                                                            ccTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAGGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCCATTGCCCTGAACCCGGTc                                                         <  5:280144/136‑1 (MQ=255)
                                                                              tGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCCAAGCCGGCGCCACCAGGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTGCCGCGCCTCGGCCATTGCCCTGCACCCGGTc                                                         >  2:303106/1‑134 (MQ=255)
                                                                                           cACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAGGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCCATTGCCCTGAACCCGGTCGACTGGGCGGACATc                                          <  6:17596/136‑1 (MQ=255)
                                                                                           cACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAAGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCCATTGCCCTGAACCCGGTCGACTGGGCGGACATc                                          <  1:156940/136‑1 (MQ=255)
                                                                                               gCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAGGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCGATTGCCCTGAACCCGGTCGCCTGGGCGGCCATCgcgc                                      >  2:124833/1‑136 (MQ=255)
                                                                                                 cTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAAGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCCATTGCCCTGAACCCGGTCGACTGGGCGGACATCGCGCTg                                    >  1:5485/1‑136 (MQ=255)
                                                                                                 cTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAAGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCCATTGCCCTGAACCCGGTCGACTGGGCGGACATCGAGCTg                                    >  3:229521/1‑136 (MQ=255)
                                                                                                     aacaacGCCCTGCGCAAAGCCGGCGACACCAAGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCCATTGCCCTGAACCCGGTCGACTGGGCGGACATCGAGCTGAcc                                 <  1:200027/135‑1 (MQ=255)
                                                                                                      acaacGCCCTGCGCAAAGCCGGCGACACCAAGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCCATTGCCCTGAACCCGGTCGACTGGGCGGACATCGAGCTGccc                                 >  8:262885/1‑134 (MQ=255)
                                                                                                             ccTGCGCAAAGCCGGCTACACCAAGAGCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCCATTGCCCTGAACCCGGTCGACTGGGCGGACATCGAGCTGACCAAGGACGcc                        <  4:117182/136‑1 (MQ=255)
                                                                                                             ccTGCGCAAAGCCGGCGACACCAGGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCCATTGCCCTGAACCCGGTCGACTGGGCGGACATCGAGCTGACCAAGGACGcc                        >  5:267623/1‑136 (MQ=255)
                                                                                                             ccTGCGCAAAGCCGGCGACACCAGGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCCATTGCCCTGAACCCGGTCGACTGGGCGGACATCGAGCTGACCAAGGACGc                         >  7:55436/1‑135 (MQ=255)
                                                                                                             ccTGCGCAAAGCCGGCGACACCAAGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCCATTGCCCTGAACCCGGTCGACTGGGCGGACATCGAGCTGACCAAGGACGc                         >  8:298204/1‑135 (MQ=255)
                                                                                                              cTGCGCAAAGCCGGCGACACCAGGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCCATTGCCCTGAACCCGGTCGACTGGGCGGACATCGAGCTGACCAAGGACGCCa                       <  1:175049/136‑1 (MQ=255)
                                                                                                              cTGCGCAAAGCCGGCGACACCAAGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCCATTGCCCTGAACCCGGTCGACTGGGCGGACATCGAGCTGACCAAGGACGCCa                       <  3:94209/136‑1 (MQ=255)
                                                                                                                gcgcAAAGCCGGCTACACCAAGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCCATTGCCCGGAACCCGGTCGACTGGGCGGACATCGAGCTGACCAAGGACGCCAAc                     <  2:284434/136‑1 (MQ=255)
                                                                                                                  gcAAAGCCGGCGACACCAGGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCCATTGCCCTGAACCCGGTCGACTGGGCGGACATCGAGCTGACCAAGGACGCCAACgg                   <  7:228381/136‑1 (MQ=255)
                                                                                                                    aaaGCCGGCGACACCAGGATCGATACTCTGCGCCGCGCGCTCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCCATTGCCCTGAACCCGGTCGACTGGGCGGACATCGAGCTGACCAAGGCCGCCCACGGCt                 >  4:32992/1‑136 (MQ=255)
                                                                                                                    aaaGCCGGCGACACCAAGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCCATTGCCCTGAACCCGGTCGACTGGGCGGACATCGAGCTGACCAAGGACGCCACCGGCt                 >  4:15303/1‑136 (MQ=255)
                                                                                                                    aaaGCCGGCGACACCAAGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCCATTGCCCTGAACCCGGTCGACGGGGCGGACATCGAGCTGACCAAGGACGCCACCGGCt                 >  6:245433/1‑136 (MQ=255)
                                                                                                                        cgggCTACACCAAGATCGATACTCTGCGCGGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCCATTGCCCTGAACCCGGTCGACTGGGCGGACATCGAGCTGACCAAGGACGCCAACGGCTCCTa             <  4:147424/134‑1 (MQ=255)
                                                                                                                                acCAAGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCCATTGCCCTGAACCCGGTCGACTGGGCGGACATCGAGCTGACCAAGGACGCCAACGGCTCCTACATCTggg     <  2:18763/136‑1 (MQ=255)
                                                                                                                                acCAAGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCCATTGCCCTGAACCCGGTCGACTGGGCGGACATCGAGCTGACCAAGGACGCCAACGGCTCCTACATCTggg     <  5:84274/136‑1 (MQ=255)
                                                                                                                                   aaGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCCATTGCCCTGAACCCGGTCGACTGGGCGGACATCGAGCTGACCAAGGACGCCAACGGCTCCTACATCTGGGTGa  <  5:108008/136‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                      
GCGCGATCTACGGCTTGAAGTACAAGGAAGAAGAGCAACTGCTCGCTGGCGACGGCACCGGCCAGAACCTGCTGGGCCTGATTCCTCAGGCCACCGCCTTCAACAACGCCCTGCGCAAAGCCGGCGACACCAAGATCGATACTCTGCGCCGCGCGATCCTGCAGGTCCGCATCGCCGAGTACCGCGCCTCGGCCATTGCCCTGAACCCGGTCGACTGGGCGGACATCGAGCTGACCAAGGACGCCAACGGCTCCTACATCTGGGTGA  >  ACB143/1754624‑1754890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: