Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 3,080,143 G→A 6.4% G148D (GGC→GAC)  cynR_2 → HTH‑type transcriptional regulator CynR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,080,1430GA 6.4% 206.9 / 3.4 62G148D (GGC→GAC) cynR_2HTH‑type transcriptional regulator CynR
Reads supporting (aligned to +/- strand):  ref base G (35/23);  new base A (2/2);  total (37/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.08e-01

CTGTGGCCAGCCGAATGCTGCCAGGGCCGCTGGCCCGCTTTGTGGCCGCGCACCCCAATGTGCAGGTCAGCATTCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTGGCGTGGTGTGCCGCAGCGACCACCCGCTGGCGCAGGCGGGCGGGCGGCTGCATTGGGCGCAGTTGCTGG  >  ACB143/3080011‑3080277
                                                                                                                                    |                                                                                                                                      
cTGTGGCCAGCCGAATGCTGCCAGGGCCGCTGGCCCGCTTTGTGGCCGCGCACCCCAATGTGCAGGTCAGCATTCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCAt                                                                                                                                     <  7:77540/136‑1 (MQ=255)
   tgGCCAGCCGAATGCTGCCAGGGCCGCTGGCCCGCTTTGTGGCCGCGCACCCCAATGTGCAGGTCAGCATTCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCAt                                                                                                                                     <  4:237751/133‑1 (MQ=255)
     gCCAGCCGAATGCTGCCAGGGCCGCTGGCCCGCTTTGTGGCCGCGCACCCCAATGTGCAGGTCAGCATTCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATcagca                                                                                                                                <  7:4640/136‑1 (MQ=255)
         gCCGAATGCTGCCAGGGCCGCTGGCCCGCTTTGTGGCCGCGCACCCCAATGTGCAGGTCAGCATTCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCAGCAGCCt                                                                                                                            >  4:259742/1‑136 (MQ=255)
           cGAATGCTGCCAGGGCCGCTGGCCCGCTTTGTGGCCGCGCACCCCAATGTGCAGGTCAGCATTCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCAGCAGCCtgt                                                                                                                          >  5:312177/1‑136 (MQ=255)
           cGAATGCTGCCAGGGCCGCTGGCCCGCTTTGTGGCCGCGCACCCCAATGTGCAGGTCAGCATTCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCAGCAGCCtgt                                                                                                                          >  6:260899/1‑136 (MQ=255)
            gAATGCTGCCAGGGCCGCTGGCCCGCTTTGTGGCCGCGCACCCCAATGTGCAGGTCAGCATTCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCAGCAGCCtgtg                                                                                                                         <  1:951/136‑1 (MQ=255)
                       gggCCGCTGGCCCGCTTTGTGGCCGCGCACCCCAATGTGCAGGTCAGCATTCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGAt                                                                                                               >  5:33008/1‑135 (MQ=255)
                               ggCCCGCTTTGTGGCCGCGCACCCCAATGTGCAGGTCAGCATTCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCagcagc                                                                                                                              >  5:117927/1‑112 (MQ=255)
                               ggCCCGCTTTGTGGCCGCGCACCCCAATGTGCAGGTCAGCATTCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCagcagc                                                                                                                              <  6:117927/112‑1 (MQ=255)
                                gCCCGCTTTGTGGCCGCGCACCCCAATGTGCAGGTCAGCATTCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTc                                                                                                      >  1:219022/1‑135 (MQ=255)
                                        tgtgGCCGCGCACCCCAATGTGCAGGTCAGCATTCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCg                                                                                               <  4:232095/134‑1 (MQ=255)
                                        tgtgGCCGCGCACCCCAATGTGCAGGTCAGCATTCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGcc                                                                                             >  6:101251/1‑136 (MQ=255)
                                             ccGCGCACCCCAATGTGCAGGTCAGCATTCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCAt                                                                                        >  7:264895/1‑136 (MQ=255)
                                                   aCCCCAATGTGCAGGTCAGCATTCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTc                                                                                                <  1:22762/122‑1 (MQ=255)
                                                   aCCCCAATGTGCAGGTCAGCATTCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTc                                                                                                >  2:22762/1‑122 (MQ=255)
                                                   aCCCCAATGTGCAGGTCAGCATTCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGaa                                                                                  >  7:270814/1‑136 (MQ=255)
                                                   aCCCCAATGTGCAGGTCAGCATTCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGaa                                                                                  >  1:184539/1‑136 (MQ=255)
                                                   aCCCCAATGTGCAGGTCAGCATTCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGaa                                                                                  >  8:273715/1‑136 (MQ=255)
                                                    ccccAATGTGCAGGTCAGCATTCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCTCTCAAGGGCAGGTGGATTTCGGCATCAGGAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAAt                                                                                 <  1:138116/136‑1 (MQ=255)
                                                        aaTGTGCAGGTCAGCATTCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATc                                                                             <  5:101251/136‑1 (MQ=255)
                                                              cAGGTCAGCATTCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCTGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTg                                                                       <  8:54142/136‑1 (MQ=255)
                                                              cAGGTCAGCATTCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTg                                                                       <  5:8931/136‑1 (MQ=255)
                                                                ggTCAGCATTCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTGGc                                                                     >  1:135185/1‑136 (MQ=255)
                                                                 gTCAGCATTCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTAAAGGGCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTGGCg                                                                    >  5:150502/1‑136 (MQ=255)
                                                                  tCAGCATTCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTGGCgt                                                                   >  4:263312/1‑136 (MQ=255)
                                                                  tCAGCATTCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTGGCgt                                                                   >  5:289845/1‑136 (MQ=255)
                                                                  tCAGCATTCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTGGCgt                                                                   >  2:79932/1‑136 (MQ=255)
                                                                    aGCATTCAGGACGGCACGGCCGAACGAGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGCATTTCGGCATCAGCAGCCTGAGGGTGGACGATGACCAGCTCGAATTCGCCCCCAACCTGAATGATCAGGTTGGCgggg                                                                 >  8:290659/1‑136 (MQ=255)
                                                                     gCATTCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTGGCgtggt                                                                <  6:56895/136‑1 (MQ=255)
                                                                         tCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTGGCGTGGTGTGc                                                            >  1:78851/1‑136 (MQ=255)
                                                                         tCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTGGCGTGGTGTGc                                                            >  8:302426/1‑136 (MQ=255)
                                                                         tCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGACATCAGCAGCCtgtg                                                                                                                         >  1:155863/1‑75 (MQ=255)
                                                                         tCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGACATCAGCAGCCtgtg                                                                                                                         >  1:151504/1‑75 (MQ=255)
                                                                         tCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGACATCAGCAGCCtgtg                                                                                                                         <  2:151504/75‑1 (MQ=255)
                                                                         tCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGACATCAGCAGCCtgtg                                                                                                                         <  2:155863/75‑1 (MQ=255)
                                                                                 gcacggcCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTGGCGTGGTGTGCCGCAGCGa                                                    <  3:60426/136‑1 (MQ=255)
                                                                                   acggcCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTGGCGTGGTGTGCCGCAGCGac                                                   >  5:138513/1‑135 (MQ=255)
                                                                                   acggcCGAACGTGTGCAGCAGTTGCTCGCTAAAGGGCAGGTGGATTTCGGCATCAGCAGCCAGTGGGTCGACGATGCCCAGCTCGAATTCGCCCCCATCGTGAATGATCAGCATGGAGTGGTGTGCCGCAGCGacc                                                  >  8:106529/1‑136 (MQ=255)
                                                                                   acggaCGAACGTGTGCAGCAGTTGCTCGCTCAAGGTCAGGTGGATTTCGGCATCCGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCTTGAAGGATCAGGTTGTCGTGGTGTGCCGCAGCGaca                                                  >  8:82750/1‑135 (MQ=255)
                                                                                     ggcCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTGGCGTGGTGTGCCGCAGCGaccac                                                >  8:185792/1‑136 (MQ=255)
                                                                                      gcCGACCGTGGGCCGCAGTTGCTCGCTCACGGGCATGTGGATTTCGGCATCAGCAGCCTGTGGGGGGACGAGGACCAGATCGCATTCGCCCCCATCGGGAATGAGCAGGTTGGCGTGGTGGGCCGCAGCGaccacc                                               <  5:227071/136‑1 (MQ=255)
                                                                                           aCGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTGGCGTGGTGTGCCGCAGCGACCACCCGCTg                                          <  2:219022/136‑1 (MQ=255)
                                                                                                   agcagTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTGGCGTGGTGTGCCGCAGCGACCACCCGCTGGCGCAggc                                  >  4:201962/1‑136 (MQ=255)
                                                                                                   agcagTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTGGCGTGGTGTGCCGCAGCGACCACCCGCTGGCGCAggc                                  >  1:63350/1‑136 (MQ=255)
                                                                                                     cagTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTGGCGTGGTGTGCCGCAGCGACCACCCGCTGGCGCAggcgg                                <  6:289845/136‑1 (MQ=255)
                                                                                                           ctcgctcAAGGGCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTGGCGTGGTGTGCCGCAGCGACCACCCGCTGGCGCAggcgggcggg                           <  6:33008/135‑1 (MQ=255)
                                                                                                             cgctcAAGGGCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTGGCGTGGTGTGCCGCAGCGACCACCCGCTGGCGCAGgcgggcgggcgg                        >  2:85669/1‑136 (MQ=255)
                                                                                                                      gCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTGGCGTGGTGTGCCGCAGCGACCACCCGCTGGCGCAGGCGGGCGGGCGGCTGCATTgg               <  2:229568/136‑1 (MQ=255)
                                                                                                                      gCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTGGCGTGGTGTGCCGCAGCGACCACCCGCTGGCGCAGGCGGGCGGGCGGCTGCATTgg               <  1:308878/136‑1 (MQ=255)
                                                                                                                      gCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTGGCGTGGTGTGCCGCAGCGACCACCCGCTGGCGCAGGCGGGCGGGCGGCTGCATTgg               <  3:219934/136‑1 (MQ=255)
                                                                                                                      gCAGGTGGATGTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTGGCGTGGTGTGCCGCAGCGACCACCCGCTGGCGCAGGCGGGCGGGCGGCTGCATTgg               <  1:273745/136‑1 (MQ=255)
                                                                                                                            ggATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTGGCGTGGTGTGCCGCAGCGACCACCCGCTGGCGCAggcgggcgggcg                         >  2:150794/1‑120 (MQ=255)
                                                                                                                            ggATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTGGCGTGGTGTGCCGCAGCGACCACCCGCTGGCGCAggcgggcgggcg                         <  1:150794/120‑1 (MQ=255)
                                                                                                                             gATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCg                                                                                      >  5:76839/1‑58 (MQ=255)
                                                                                                                             gATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCg                                                                                      <  6:76839/58‑1 (MQ=255)
                                                                                                                             gATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTGGCGTGGTGTGCCGCAGCGACCCCCCGCTGGCGCAGGCGGGCGGGCGGCTGCATTGGGCGCAGt        >  3:5254/1‑136 (MQ=255)
                                                                                                                             gATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTGGCGTGGTGTGCCGCAGCGACCACCCGCTGGCGCAGGCGGGCGGGCGGCTGCATTGGGCGCAGt        >  1:87311/1‑136 (MQ=255)
                                                                                                                             gATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTGGCGTGGTGTGCCGCAGCGACCACCCGCTGGCGCAGGCGGGCGGGCGGCTGCATTGGGCGCAGt        >  1:21137/1‑136 (MQ=255)
                                                                                                                             gATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTGGCGTGGTGTGCCGCAGCGACCACCCGCTGGCGCAGGCGGGCGGGCGGCTGCATTGGGCGCAGt        >  5:149253/1‑136 (MQ=255)
                                                                                                                             gATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTGGCGTGGTGTGCCGCAGCGACCACCCGCTGGCGCAGGCGGGCGGGCGGCTGCATTGGGCGCAGt        >  5:144452/1‑136 (MQ=255)
                                                                                                                             gATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTGGCGTGGTGTGCCGCAGCGACCACCCGCTGGCGCAGGCGGGCGGGCGGCTGCATTGGGCGCAGt        >  3:99674/1‑136 (MQ=255)
                                                                                                                                   ggCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTGGCGTGGTGTGCCGCAGCGACCACCCGCTGGCGCAGGCGGGCGGGCGGCTGCATTGGGCGCAGTTGCTgg  <  5:121286/136‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                      
CTGTGGCCAGCCGAATGCTGCCAGGGCCGCTGGCCCGCTTTGTGGCCGCGCACCCCAATGTGCAGGTCAGCATTCAGGACGGCACGGCCGAACGTGTGCAGCAGTTGCTCGCTCAAGGGCAGGTGGATTTCGGCATCAGCAGCCTGTGGGTGGACGATGACCAGCTCGAATTCGCCCCCATCGTGAATGATCAGGTTGGCGTGGTGTGCCGCAGCGACCACCCGCTGGCGCAGGCGGGCGGGCGGCTGCATTGGGCGCAGTTGCTGG  >  ACB143/3080011‑3080277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑