Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,245,128 A→C 32.6% L178R (CTG→CGG)  CJ019_03982 ← Crotonyl‑CoA hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,245,1280AC32.6% 25.9 / 8.8 53L178R (CTG→CGG) CJ019_03982Crotonyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base A (13/15);  new base C (12/6);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 2.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

GGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGT  >  ACB143/4244995‑4245248
                                                                                                                                     |                                                                                                                        
ggCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCGGACCAACCCGGCGGCCCgg                                                                                                                        >  1:315409/1‑136 (MQ=255)
    ccGGCGCGGATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGgcgc                                                                                                                    <  8:8761/136‑1 (MQ=255)
       tCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGAt                                                                                                                 <  5:1072/136‑1 (MQ=255)
       tCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGGCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGAt                                                                                                                 <  4:151758/136‑1 (MQ=255)
                    gCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGCTCGGCGCCCCCCaa                                                                                                    >  5:94178/1‑136 (MQ=255)
                        ggTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCCGGCGCGATCGGCGCCCCCCAACtcc                                                                                                >  6:206140/1‑135 (MQ=255)
                        ggTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACtcg                                                                                                >  3:229580/1‑136 (MQ=255)
                          tCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACtcgtc                                                                                              >  7:97916/1‑136 (MQ=255)
                                 aTAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGCCCACCCCGGCGGCCCGGGCGCGATCGGCGCCCCCCACCGCGGCGAggca                                                                                        >  7:229005/1‑133 (MQ=255)
                                 aTAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCCGGCCCGCTCGGCGCCCCCCCACACGGCGAggca                                                                                        >  4:133187/1‑133 (MQ=255)
                                  tAGGCGAAGGTCGGGCCATTGGCCTGGCGCCGGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGGGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                      <  2:27707/136‑1 (MQ=255)
                                  tAGGCGAAGGTCGGGCCATTGGCCTGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACAAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                      <  4:119318/136‑1 (MQ=255)
                                   aGGCGAAGGTCGGGCCTTGGGCCAGGCGCCCGGCCTGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCTGGGCGCTATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                      <  4:114684/135‑1 (MQ=255)
                                      cGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACAACTCGGCGAGGAAccgcag                                                                                  >  8:120562/1‑136 (MQ=255)
                                       gAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAgg                                                                                 <  1:28368/136‑1 (MQ=255)
                                       gAAGGGCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCTGGGCGCGATCGGGGCCCCACAACTCGTCGAGGAACAGCAgg                                                                                 <  3:305294/136‑1 (MQ=255)
                                           gTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTGGAGGAACAGCAGGCGCt                                                                             >  4:247487/1‑136 (MQ=255)
                                           gTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTAGAGGAACAGCAGGCGCt                                                                             >  7:44472/1‑136 (MQ=255)
                                           gTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCACGGGGCGCTCGGCGCCCCCCAACAAGGCGAGGGACAGCAGGCGCt                                                                             >  4:46665/1‑136 (MQ=255)
                                                 ccATTGGCCAGGCGCCCGGCCTGTTGGGCGGAAAGGGCAGGAATCTGCTGGGCGGCGCAGACTTCGGTGACCAACCCGCGGGCCTGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCt                                                                       <  6:216525/136‑1 (MQ=255)
                                                 ccATTGGCCAGGCGCCCGGCCAGTTGGGCGGCAACGGCAGGCAACTGCTCGGCGGCCCTGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCt                                                                       <  2:172523/136‑1 (MQ=255)
                                                  cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCCCCACGCGTCGAGGAACCGCAGGCGCTTGGCCTg                                                                      >  1:251440/1‑136 (MQ=255)
                                                  cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCCCCACGCGTCGAGGAACAGCAGGCGCGTGGCCTg                                                                      >  8:176359/1‑136 (MQ=255)
                                                  cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCCCCACGCGGCGAGGAACAGCCGGCGCGTGGccag                                                                      >  5:81574/1‑134 (MQ=255)
                                                  cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTg                                                                      >  2:235851/1‑136 (MQ=255)
                                                  cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCCGGGGCCCTACGCGCCCCCCAACACCGCGCGGGACCCCACGCGCGTGGccgg                                                                      >  1:133967/1‑134 (MQ=255)
                                                  cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCCCAACTCGTCGGGGCACCGCAGGCGCGTGGccgg                                                                      >  7:73572/1‑134 (MQ=255)
                                                  cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCCCAACTCGTCGCGGAACCCCAGGCGCTTGGCCTg                                                                      >  5:83575/1‑136 (MQ=255)
                                                  cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTg                                                                      >  1:246923/1‑136 (MQ=255)
                                                        ccAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGCCTTCGCTGACCAACCCGGCGGCCCGGGGCCGCACGGCGCCCCCCCACACGGCGCGGAACAACAGGCGGGTGGCCAGcgaccg                                                                >  8:270779/1‑131 (MQ=255)
                                                        ccAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCCCCACGCGTCGGGGAACAGCAGGCGCTTGGCCTGCTCGCt                                                                >  8:29035/1‑136 (MQ=255)
                                                           ggCGCCCGGCCAGTTCGGCGGCAAGGGCAGGCAACTGCTCGGCGGCGAAGACTGCGCTGACCAACCCGGCGGCCTGGGCGCGATGGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                             <  8:51767/136‑1 (MQ=255)
                                                           ggCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCGCACCAACCCGGCGGCCTGGGCGCGATCGGCCCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                             <  8:261328/136‑1 (MQ=255)
                                                            gcgcCGGGCCAGTGCGGGGGCACGGGCGGGCAAGGGCGGGGCGGCGCATACTGCGGTGACCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGTAACAGCAGGCGCTTGGCCTGCTCGCTGc                                                              <  2:315409/134‑1 (MQ=255)
                                                                     cAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCGGACCAACCCGGGGGCCAGGGCGCTATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGt                                                   <  8:45452/136‑1 (MQ=255)
                                                                     cAGGTCGGCGGCAAGGGCAGGCAACTGCTCGGCGGCGCAGACTTCGCGGACCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGt                                                   <  5:304057/136‑1 (MQ=255)
                                                                                    ggcaggcaCCTGCTCGGCGGCGCAGACTTCGCTGACCACCCCGGCGGCCTGGGCGCGATGGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTg                                    <  5:161420/136‑1 (MQ=255)
                                                                                           aaCTGCCCGTCGGCGCAGAATTCGCTGACAAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCgg                             <  4:310797/136‑1 (MQ=255)
                                                                                             cTGCGCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                           <  3:278980/136‑1 (MQ=255)
                                                                                             cTGCACGTCGGCGCAGACTTCGCTGCCCAACCCGCGGGCCCGGGCGCGATGGGCGCCCCACAACTCGTCGAGGCACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                           <  6:165368/136‑1 (MQ=255)
                                                                                                 tcgtcgGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCCCAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCCGCTGGCGCCGGCATCgg                       >  5:269495/1‑136 (MQ=255)
                                                                                                 tcgtcgGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCGGGCGCCGGCATCgg                       >  4:205418/1‑136 (MQ=255)
                                                                                                  cggggccgcATACTTCGCCCCCCACCCGGGGGGCGCGGGCGGGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCggg                      <  4:165124/130‑1 (MQ=255)
                                                                                                  cggcgGCGCAGACGTCGCTGCCCACCCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCggg                      <  6:267637/133‑1 (MQ=255)
                                                                                                       gcgcAGACTGCGCGCACCACCCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGt                 <  6:30741/136‑1 (MQ=255)
                                                                                                        cgcAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCCCAACTCGTCGCGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCCTCGGGGCTg                  >  3:301013/1‑134 (MQ=255)
                                                                                                           aGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCCCCACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCCGCCGTGGCAGGTTCCCGCTGGCGCCGGCATCGGGGCTGTAGcc             >  2:168495/1‑136 (MQ=255)
                                                                                                                      cccACCCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  2:158290/135‑1 (MQ=255)
                                                                                                                      cccAACCGGCCGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  7:56220/135‑1 (MQ=255)
                                                                                                                      accaccCGGGCGGCCCGGGGGCTAGCGGCCCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  2:133967/136‑1 (MQ=255)
                                                                                                                      aCCAACCCGGCGGCCTGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  5:309082/136‑1 (MQ=255)
                                                                                                                      aCCAACCCGGCGGCCCGGGCGCGTGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  3:133187/136‑1 (MQ=255)
                                                                                                                      aCCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  3:260423/136‑1 (MQ=255)
                                                                                                                                     |                                                                                                                        
GGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGT  >  ACB143/4244995‑4245248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑