New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? ACB143 1731043 =73 (1.220)12 (0.230) 10/228 2.9 17.2% coding (110/426 nt) spxA Regulatory protein Spx
?ACB143 = 1770457 52 (1.000)intergenic (+135/+157) CJ019_01684/higA‑1 hypothetical protein/Antitoxin HigA‑1
Rejected: Frequency below/above cutoff threshold.

GATCTATACCGTGACAGCCGTGGCGCTGAGTGGATCGATGTCGCATAATCCTGCGACTAATTTTTGGGAGATATTGGGGAGGTTTTGGGGAGTAATCCCAGCCCCTTTAAATTCAATGACTTACACTCTTTACGGCATCAAAGCCTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  ACB143/1731189‑1731043
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tacggcatcaaagcctgGGACGCCAAGTTAAACCGACTAAAGCCCAGTTAAATCAATGCCTTAAAGCAAATATGGCGCATCGGAATTGCGCCTTTTTTGTTCGTTTCGACCCCAATAGAATCAGGTGTTTACGGATGCATTTTGGGG  <  ACB143/1770457‑1770328
                                                                                                                                                                                                                                                                                     
GATCTATACCGTGACAGCCGTGGCGCTGAGTGGATCGATGTCGCATAATCCTGCGACTAATTTTTGGGAGATATTGGGGAGGTTTTGGGGAGTAATCCCAGCCCCTTTAAATTCAATGAGTTACACTCTTTACGG                                                                                                                                                >  6:8465/1‑135
   CTATACCGTGCCAGCCGTGGAGCTGAGTGTATCGATGTCGAATAATCCTGCGACTAATTTTTGGGAGATATTGGGGAGGTGTTGGGGAGTAATCCCAGCCCCTTTAAATTCAATGACTTACACTCTTTACGGCATC                                                                                                                                            <  8:345974/136‑1
      TACCGTGACAGCCGTGGCGCTGAGTGGATCGATGTCGCATAATCCTGCGACTAATTTTTGGGAGATATTGGGGAGGTTTTGGGGAGTAATCCCAGCCCCTTTAAATTCAATGACTTACACTCTTTACGGCATC                                                                                                                                            <  5:349600/133‑1
      TACCGTGACAGCCGTGGCGCTGAGTGGATCGATGTCGCATAATCCTGCGACTAATTTTTGGGAGATATTGGGGAGGTTTTGGGGAGTAATCCCAGCCCCTTTAAATTCAATGACTTACACTCTTTACGGCA                                                                                                                                              <  1:319523/131‑1
      TACCGTGACAGCCGTGGCGCTGAGTGGATCGATGTCGCATAATCCTGCGACTAATTTTTGGGAGATATTGGGGAGGTTTTGGGGAGTAATCCCAGCCCCTTTAAATTCAATGACTTACACTCTTTACGGCA                                                                                                                                              <  7:296642/131‑1
      TACCGTGACAGACGTGGCGCTGAGTGGATCGATGTCGCATAATCCTGCGACTAATTTTTGGGAGATATTGGGTAGGTTTTGGGGAGTAATCCCAGCCCCTTTAAATTCAATGACTTACACTCTTTACGGC                                                                                                                                               <  8:29657/130‑1
         CGTGACAGCCGTGGCGCTGAGTGGATCGATGTCGCATAATCCTGCGACTAATTTTTGGGAGATATTGGGGAGGTTTTGGGGAGTAATCCCAGCCCCTTTAAATTCAATGACTTACACTCTTTACGGCATCAAAGCC                                                                                                                                      >  1:316909/1‑136
         CGTGACAGCCGTGGCGCTGAGTGGATCGATGTCGCATAATCCTGCGACTAATTTTTGGGAGATATTGGGGAGGTTTTGGGGAGTAATCCCAGCCCCTTTAAATTCAATGACTTACACTCTTTACGGCATCAAAGCC                                                                                                                                      <  1:78917/136‑1
           TGACAGCCGTGGCGCTGAGTGGATCGATGTCGCATAATCCTGCGACTAATTTTTGGGAGATATTGGGGAGGTTTTGGGGAGTAATCCCAGCCCCTTTAAATTCAATGACTTACACTCTTTACGGCATCAAAGCCTG                                                                                                                                    <  7:82857/136‑1
            GACAGCCGTGGCGCTGAGTGGATCGATGTCGCATAATCCTGCGACTAATTTTTGGGAGATATTGGGGAGGTTTTGGGGAGTAATCCCAGCCCCTTTAAATTCAATGACTTACACTCTTTACGGCATCAAAGCCTGG                                                                                                                                   >  6:215081/1‑136
              CAGCCGTGGCGCTGAGTGGATCGATGTCGCATAATCCTGCGACTAATTTTTGGGAGATATTGGGGAGGTTTTGGGGAGTAATCCCAGCCCCTTTAAATTCAATGACTTACACTCTTTACGGCATCAAAGCCTTGGA                                                                                                                                 >  8:71898/1‑136
              CAGCCGTGGCGCTGAGTGGATCGATGTCGCATAATCCTGCGACTAATTTTTGGGAGATATTGGGGAGGTTTTGGGGAGTAATCCCAGCCCCTTTAAATTCAATGACTTACACTCTTTACGGCATCAAAGCCTGGGA                                                                                                                                 >  3:193785/1‑136
               AGCCGTGGCGCTGAGTGGATCGATGTCGCATAATCCTGCGACTAATTTTTGGGAGATATTGGGGAGGTTTTGGGGAGTAATCCCAGCCCCTTTAAATTCAATGACTTACACTCTTTACGGCATCAAAGCCTGCGAC                                                                                                                                >  6:256269/1‑136
                                  TCGATGTCGCATAATCCTGCGACTAATTTTTGGGAGATATTGGGGAGGTTTTGGGGAGTAATCCCAGCCCCTTTAAATTCAATGACTTACACTCTTTACGGCATCAAAGCCTGGGACGCCAAGTTAAACCGACTAA                                                                                                             <  6:276160/136‑1
                                    GATGTCGCATAATCCTGCGACTAATTTTTGGGAGATATTGGGGAGGTTTTGGGGAGTAATCCCAGCCCCTTTAAATTCAATGACTTACACTCTTTACGGCATCAAAGCCTGGGACGCCAAGTTAAACCGACTAAAG                                                                                                           >  4:95413/1‑136
                                              AATCCTGCGACTAATTTTTGGGAGATATTGGGGAGGTTTTGGGGAGTAATCCCAGCCCCTTTAAATTCAATGACTTACACTCTTTACGGCATCAAAGCCTGGGACGCCAAGTTAAACCGACTAAAGCCCAGTTAAA                                                                                                 >  2:362724/1‑136
                                               ATCCTGCGACTAATTTTTGGGAGATATTGGGGAGGTTTTGGGGAGTAATCCCAGCCCCTTTAAATTCAATGACTTACACTCTTTACGGCATCAAAGCCTGGGACGCCAAGTTAAACCGACTAAAGCCCAGTTAAAT                                                                                                <  6:350076/136‑1
                                                         TAATTTTTGGGAGATATTGGGGAGGTTTTTGGGAGTAATCCCAGCCCCTTTAAATTCAATGACTTACACTCTTTACGGCATCAAAGCCTGGGACGCCAAGTTAAACCGACTAAAGCCCAGTTAAATCAATGCCTTA                                                                                      <  2:255857/136‑1
                                                           ATTTTTGGGAGATATTGGGGAGGTTTTGGGGAGTAATCCCAGCCCCTTTAAATTCAATGACTTACACTCTTTACGGCATCAAAGCCTGGGACGCCAAGTTAAACCGACTAAAGCCCAGTTAAATCAATGCCTTAA                                                                                     <  1:362724/135‑1
                                                                         TTGGGGAGGTTTTGGGGAGTAATCCCAGCCCCTTTAAATTCAATGACTTACACTCTTTACGGC                                                                                                                                               >  2:232411/1‑63
                                                                         TTGGGGAGGTTTTGGGGAGTAATCCCAGCCCCTTTAAATTCAATGACTTACACTCTTTACGGC                                                                                                                                               <  1:232411/63‑1
                                                                                    TTGGGGAGTAATCCCAGCCCCTTTAAATTCAATGACTTACACTCTTTACGGCATCAAAGCCTGGGACGCCAAGTTAAACCGACTAAAGCCCAGTTAAATCAATGCCTTAAAGCAAATATGGCGCATCGGAATTGCG                                                           >  2:309434/1‑136
                                                                                                                 CAATGACTTACACTCTTTACGGCATCAAAGCCTGGGACGCCAAGTTAAACCGACTAAAGCCCAGTTAAATCAATGCCTTAAAGCAAATATGGCGCATCGGAATTGCGCCTTTTTTGTTCGTTTCGACCCCAATAG                               <  4:193785/135‑1
                                                                                                                                   ACGGCATCAAAGCCTGGGACGCCAAGTTAAACCGACTAAAGCCCAGTTAAATCAATGCCTTAAAGCAAATATGGCGCATCGGAATTGCGCCTTTTTTGTTCGTTTCGACCCCAATAGAATCAGGTGTTTACGGATG            >  2:111992/1‑136
                                                                                                                                   ACGGCATCAAAGCCTGGGACGCCAAGTTAAACCGACTAAAGCCCAGTTAAATCAATGCCTTAAAGCAAATATGGCGCATCGGAATTGCGCCTTTTTTGTTCGTTTCGACCCCAATAGAATCAGGTGTTTACGGATG            >  5:221919/1‑136
                                                                                                                                    CGGCATCAAAGCCTGGGACGCCAAGTTAAACCGACTAAAGCCCAGTTAAATCAATGCCTTAAAGCAAATATGGCGCATCGGAATTGCGCCTTTTTTGTTCGTTTCGACCCCAATAGAATCAGGTGTTTACGGAT             <  1:17196/134‑1
                                                                                                                                     GGCATCAAAGCCTGGGACGCCAAGTTAAACCGACTAAAGCCCAGTTAAATCAATGCCTTAAAGCAAATATGGCGCATCGGAATTGCGCCTTTTTTGTTCGTTTCGACCCCAATAGAATCAGGTGTTTACGGATGCA          >  6:91536/1‑136
                                                                                                                                     GGCATCAAAGCCTGGGACGCCAAGTTAAACCGACTAAAGCCCAGTTAAATCAATGCCTTAAAGCAAATATGGCGCATCGGAATTGCGCCTTTTTTGTTCGTTTCGACCCCAATAGAATCAGGTGTTTACGGATGCA          >  4:63120/1‑136
                                                                                                                                     GGCATCAAAGCCTGGGACGCCAAGTTAAACCGACTAAAGCCCAGTTAAATCAATGCCTTAAAGCAAATATGGCGCATCGGAAGTGAGCCTTTTTTGTTCTTTTCGACCCCAATATAATCAGGTGTTGACGGATGCA          >  7:24873/1‑136
                                                                                                                                     GGCATCAAAGCCTGGGACGCCAAGTTAAACCGACTAA                                                                                                             <  4:56150/37‑1
                                                                                                                                     GGCATCAAAGCCTGGGACGCCAAGTTAAACCGACTAA                                                                                                             >  3:56150/1‑37
                                                                                                                                      GCATCAAAGCCTGGGACGCCAAGTTAAACCGACTAAAGCCCAGTTAAATCAATGCCTTAAAGCAAATATGGCGCATCGGAATTGCGCCTTTTTTGTTCGTTTCGACCCCAATAGAATCAGGTGTTTACGGATGCAT         >  2:9785/1‑136
                                                                                                                                          CAAAGCCTGGGACGCCAAGTTAAACCGACTAAAGCCCAGTTAAATCAATGCCTTAAAGCAAATATGGCGCATCGGAATTGCGCCTTTTTTGTTCGTTTCGACCCCAATAGAATCAGGTGTTTACGGATGCATTTTG     >  7:269336/1‑136
                                                                                                                                           AAAGCCTGGGACGCCAAGTTAAACCGACTAAAGCCCAGTTAAATCAATGCCTTAAAGCAAATATGGCGCATCGGAATTGCGCCTTTTTTGTTCGTTTCGACCCCAATAGAATCAGGTGTTTACGGATGCATTTTGG    >  2:176675/1‑136
                                                                                                                                           AAAGCCTGGGACGCCAAGTTAAACCGACTAAAGCCCAGTTAAATCAATGCCTTAAAGCAAATATGGCGCATCGGAATTGCGCCTTTTTTGTTCGTTTCGACCCCAATAGAATCAGGTGTTTACGGATGCATTTTG     <  5:129723/135‑1
                                                                                                                                           AAAGCCTGGGACGCCAAGTTAAACCGACTAAAGCCCAGTTAAATCAATGCCTTAAAGCAAATATGGCGCATCGGAATTGCGCCTTTTTTGTTCGTTTCGACCCCAATAGAATCAGGTGTTTACGGATGCATTTTG     <  1:284877/135‑1
                                                                                                                                             AGCCTGGGACGCCAAGTTAAACCGACTAAAGCCCAGTTAAATCAATGCCTTAAAGCAAATATGGCGCATCGGAATTGCGCCTTTTTTGTTCGTTTCGACCCCAATAGAATCAGGTGTTTACGGATGCATTTTGGG   >  7:266241/1‑135
                                                                                                                                             AGCCTGGGACGCCAAGTTAAACCGACTAAAGCCCAGTTAAATCAATGCCTTAAAGCAAATATGGCGCATCGGAATTGCGCCTTTTTTGTTCGTTTCGACCCCAATAGAATCAGGTGTTTACGGATGCATTTTGGG   >  1:304781/1‑135
                                                                                                                                             AGCCTGGGACGCCAAGTTAAACCGACTAAAGCCCAGTTAAATCAATGCCTTAAAGCAAATATGGCGCATCGGAATTGCGCCTTTTTTGTTCGTTTCGACCCCAATAGAATCAGGTGTTTACGGATGCATTTTGGG   >  8:141691/1‑135
                                                                                                                                             AGCCTGGGACGCCAAGTTAAACCGACTAAAGCCCAGTTAAATCAATGCCTTAAAGCAAATATGGCGCATCGGAATTGCGCCTTTTTTGTTCGTTTCGACCCCAATAGAATCAGGTGTTTACGGATGCATTTTGGG   >  5:227701/1‑135
                                                                                                                                             AGCCTGGGACGCCAAGTTAAACCGACTAAAGCCCAGTTAAATCAATGCCTTAAAGCAAATATGGCGCATCGGAATTGCGCCTTTTTTGTTCGTTTCGACCCCAATAGAATCAGGTGTTTACGGATGCATTTTGGG   >  3:156735/1‑135
                                                                                                                                             AGCCTGGGACGCCAAGTTAAACCGACTAAAGCCCAGTTAAATCAATGCCTTAAAGCAAATATGGCGCATCGGAATTGCGCCTTTTTTGTTCGTTTCGACCCCAATAGAATCAGGTGTTTACGGATGCATTTTGGG   >  4:74901/1‑135
                                                                                                                                             AGCCTGGGACGCCAAGTTAAACCGACTAAAGCCCAGTTAAATCAATGACTTAAAGCAAATATGGCGCATCGGAAATGCGCCTTTTTTCTACGTTTCGACCCCAATAGAATCAGGTCTTTACGGATGCATTTTGGG   >  8:250045/1‑135
                                                                                                                                               CCTGGGACGCCAAGTTAAACCGACTAAAGCCCAGTTAAATCAATGCCTTAAAGCAAATATGGCGCATCGGAATTGCGCCTTTTTTGTTCGTTTCGACCCCAATAGAATCAGGTGTTTACGGATGCATTTTGGGG  >  4:182793/1‑134
                                                                                                                                               CCTGGGACGCCAAGTTAAACCGACTAAAGCCCAGTTAAATCAATGCCTTAAAGCAAATATGGCGCATCGGAATTGCGCCTTTTTTGTTCGTTTCGACCCCAATAGAATCAGGTGTTTACGGATGCATTTTGGGG  >  1:190320/1‑134
                                                                                                                                                                                                                                                                                     
GATCTATACCGTGACAGCCGTGGCGCTGAGTGGATCGATGTCGCATAATCCTGCGACTAATTTTTGGGAGATATTGGGGAGGTTTTGGGGAGTAATCCCAGCCCCTTTAAATTCAATGACTTACACTCTTTACGGCATCAAAGCCTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  ACB143/1731189‑1731043
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tacggcatcaaagcctgGGACGCCAAGTTAAACCGACTAAAGCCCAGTTAAATCAATGCCTTAAAGCAAATATGGCGCATCGGAATTGCGCCTTTTTTGTTCGTTTCGACCCCAATAGAATCAGGTGTTTACGGATGCATTTTGGGG  <  ACB143/1770457‑1770328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.