Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1431,113,7580CG33.3% 83.1 / 10.8 50F49L (TTC→TTG) lptFLipopolysaccharide export system permeaseprotein LptF
Reads supporting (aligned to +/- strand):  ref base C (9/23);  new base G (16/0);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.01e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATCTGTCCCGCGAGGTCCTGGTCACCTTGAGCGCCGTCAGCGCTGTGCTGTTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGCCTCTGGGGCTGTTCCTCGGCATCCTCCTGGCTTACGGGCGCCTGTACCTGGAAAGCGAGATGACCGTGCTGTCGGCCACTGGCAT  >  ACB143/1113628‑1113889
                                                                                                                                  |                                                                                                                                   
aTCTGTCCCGCGAGGTCCTGGTCACCTTGAGCGCCGTCAGCGCTGTGCTGTTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGa                                                                                                                                 <  6:321255/135‑1 (MQ=255)
  gtGTCCCGCGAGGTCCTGGTCACCTTGAGCGCCGTCAGCGCTGTGCTGTTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATc                                                                                                                               <  6:305722/134‑1 (MQ=255)
        cgcgAGGTCCTGGTCACCTTGAGCGCCGTCAGCGCTGTGCTGTTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTGCTGATCATGGGCt                                                                                                                        >  2:238847/1‑136 (MQ=255)
          cgAGGTCCTGGTCACCTTGAGCGCCGTCAGCGCTGTGCTGTTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTGCTGATCATGGGCTTc                                                                                                                      >  4:146577/1‑136 (MQ=255)
          cgAGGTCCTGGTCACCTTGAGCGCCGTCAGCGCTGTGCTGTTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTc                                                                                                                      <  2:103124/136‑1 (MQ=255)
                  tGGTCACCTTGAGCGCCGTCAGCGCTGTGCTGTTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGTGCTTGCTCGACCCGGGCGTGTTGTTGAAGCTAATCGGCTTACGCTTGc                                                                                                               >  6:251785/1‑135 (MQ=255)
                    gTCACCTTGAGCGCCGTCAGCGCTGTGCTGTTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGGTGTTGCTGATCATGGGCTTCCGCATGGCAg                                                                                                            >  1:255812/1‑136 (MQ=255)
                    gTCACCTTGAGCGCCGTCAGCGCTGTGCTGTTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGAGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAg                                                                                                            <  8:159651/136‑1 (MQ=255)
                        ccTTGAGCGCCGTCAGCGCTGTGCTGTTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCtt                                                                                                        <  1:156460/136‑1 (MQ=255)
                            gAGCGCCGTCAGCGCTGTGCTGTTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGGTGTTGCTGATAATGGGCTTACGCTTGCCAGGCTTCCTg                                                                                                    >  1:22885/1‑136 (MQ=255)
                             aGCGCCGTCAGCGCTGTGCTGTTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTg                                                                                                    <  7:25919/135‑1 (MQ=255)
                                  cGTCAGCGCTGTGCTGTTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTg                                                                                              <  5:298281/136‑1 (MQ=255)
                                    tCAGCGCTGTGCTGTTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGAt                                                                                            <  1:195140/136‑1 (MQ=255)
                                    tCAGCGCTGTGCTGTTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGAt                                                                                            <  8:189029/136‑1 (MQ=255)
                                    tCAGCGCTGTGCTGTTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGAt                                                                                            <  8:133279/136‑1 (MQ=255)
                                         gctgtgctgtTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGc                                                                                       <  4:367090/136‑1 (MQ=255)
                                               ctgtTGTGGCTCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCGAGGCGGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGCCTCTgg                                                                                 <  6:289133/136‑1 (MQ=255)
                                               ctgtTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATTGGCTTCCGCGTTCCCGGCGTCCTGCAAATGATTCTGCCTCTgg                                                                                 >  2:351565/1‑136 (MQ=255)
                                                tgtTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGGTGTTGCTGTTGATTGGGGTCCGCGTGACACGCTTCCGGCCACTGATTCTGCCGCTggg                                                                                >  2:164751/1‑136 (MQ=255)
                                                  ttGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGCCTCTggg                                                                                <  4:172389/134‑1 (MQ=255)
                                                     gTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTGCTGATCATGGGCGTGCGCTTGCCAGGCTTCCTGCAACTGAATATGCCTCTGGGGCTGt                                                                           >  5:164008/1‑136 (MQ=255)
                                                     gTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTACTGATCATGGGCTTCCGCTTGCCAGGCTTACTGCAACTGATTATTCCTCTGGGGCTGt                                                                           >  4:101574/1‑136 (MQ=255)
                                                          catcatGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTGCTGATAAAGGGCTTGCGCTTTGCACGCTTCCTGGAACTGATTCTGCCAGAGGGGCTGTTCCTc                                                                      >  2:90305/1‑136 (MQ=255)
                                                          catcatGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTACTGATCATTGGCTTCCGCTTGCCAGGCTTACTGCAACTGATTCTGCCTCTGGGGCTGTTCCTc                                                                      >  8:38299/1‑136 (MQ=255)
                                                          catcatGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGGTGTTGCTGGTGATGGGCGTCCGCGTGGCAGGCGTGCTGCAACAGATTCTGCCGCTGGGGCTGTTGCTc                                                                      >  4:77368/1‑136 (MQ=255)
                                                          catcatGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGGTGTTCCTGATCAAGGGCTTCCGCTTGCCAGGCGTCCTGCAACTGATTCTGCCTCTGGGGCTGTTGCta                                                                      >  6:97721/1‑135 (MQ=255)
                                                           atcatGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGCCTCTGGGGCTGTTCCTCg                                                                     <  4:333425/136‑1 (MQ=255)
                                                           atcatGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGCCTCTGGGGCTGTTCCTCg                                                                     <  1:92608/136‑1 (MQ=255)
                                                           atcatGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGCCTCTGGGGCTGTTCCTCg                                                                     <  2:317160/136‑1 (MQ=255)
                                                                  gCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGGTGTTGCTGATGATGGGGGTACGCATGCCACGCGTCCTGGAACTGCTTTTGCCGCTGGGGGGGTTGCTCGGCaaac                                                               >  8:218653/1‑132 (MQ=255)
                                                                     ggCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGCCTCTGGGGCTGTTCCTCGGCATcctcct                                                           <  2:195324/136‑1 (MQ=255)
                                                                        gcTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGGTGTTGGTGGTCATGGGCTTGCGCGTGCCAGGGGTGCTGCAACAGATTGTGGCTCTGGGGGTGTTCCGCGGCCTCCGCCAggg                                                        >  2:77809/1‑135 (MQ=255)
                                                                                aaGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGCCTCTGGGGCTGTTCCTCGGCATCCTCCTGGCTTACGGgc                                                <  6:51938/136‑1 (MQ=255)
                                                                                           ccAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCGTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGCCTCTGGGGCTGTTCCTCGGCATCCTCCTGGCTTACGGGCGCCTGTACCTg                                     <  1:150727/136‑1 (MQ=255)
                                                                                                  gCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGCCTCTGGGGCTGTTCCTCGGCATCCTCCTGGCTTACGGGCGCCTGTACCTGGAAAGCg                              >  8:121014/1‑136 (MQ=255)
                                                                                                     cAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGCCTCTGGGGCTGTTCCTCGGCATCCTCCTGGCTTACGGGCGCCTGTACCTGGAAAGCgaga                           <  8:306998/136‑1 (MQ=255)
                                                                                                      aGGGCGTGCTCGACCCGGGCGTGGTGGTGCTGGTGATTGGGGTACGCGTGCCCGGGGTGCTGCCACTGATTGTGCGGCTGGGGGGGTTGCTCGGCATCCTCCAGGGTGTCGGGGGCCGGTAACTGGAAAGCGAGAt                          >  4:252151/1‑136 (MQ=255)
                                                                                                           gTGCTCGACCCGGGCGTGTTGTTCCTGATGATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTTATTCTGCCTCTGGGGGTGTTCCTCGGCATCCTCCAGGCTTACGGGCGCCTGTACCTGGAAAGCGAGATGACCg                     >  6:318247/1‑136 (MQ=255)
                                                                                                                cGACCCGGGCGTGTTGTTGCAGATCAAGGGCTTCCCCTTTACAGGCTGCCTGGAACATCATATTACACAGGGGCTGTTACAACGCAAACACCAGGCTTAAGGGCGCCAGTACCAGGAAAGCCAGAAGAcccagcgg                >  4:285398/1‑130 (MQ=255)
                                                                                                                cGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGCCTCTGGGGGTGTTCCTCGGCATCCTCCAGGCTTACGGGCGCCTGTACCTGGAAAGCGAGATGACCGTGCTg                >  8:115296/1‑136 (MQ=255)
                                                                                                                cGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGCCTCTGGGGCTGTTCCTCGGCATCCTCCTGGCTTACGGGCGCCTGTACCTGGAAATCGAGATGACCGTGCTg                >  8:270619/1‑136 (MQ=255)
                                                                                                                cGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGCCTCTGGGGCTGTTCCTCGGCATCCTCCTGGCTTACGGGCGCCTGTACCTGGAAAGCGAGATGACCGTGCTg                >  8:330620/1‑136 (MQ=255)
                                                                                                                cGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCGTGCCAGGCTTCCTGCAACTGATTCTGCCTCTGGGGCTGTTCCTCGGCATCCTCCGGGCGTACGGGCGCCGGGACCTGGAAAGCGAGATGACCGTGCTg                >  8:121990/1‑136 (MQ=255)
                                                                                                                cGACCCGGGCGTGTGGTTGCTGATCATGGGCTTCCGCTTGCCAGGCGTCCAGCAAAAGATTATGCCTCGGGGGCTGTTGCTCGGCATCCCCCTGGCGTACGGGCGCCGGTTACAGGAAATCGAGAAGACCGGGCTg                >  8:57362/1‑136 (MQ=255)
                                                                                                                cGACCCGGGCGTGGTGTTGCTGATCATGGGGGTCCGCTTGCCCGGCGTCCTGCAACTGATTCTGCCGCTGGGGGTGTTACTCGGCATCCTCCTGGCTTACGGGCGCCTGTACCTGGAAAGCGAGCTGACCGTGCTg                >  8:24507/1‑136 (MQ=255)
                                                                                                                cGACCCGGGCGTGGTGTTGCGGATCATGGGCTTCCGCTTGCCAGGCGTCCGGCAACTGATTCTGCCTCTGGGGGTGTTCCTCGGGATCCTCCTGGGGTACGGGCGCCGGTTCCTGGAAAGCGAGATGACCGTGCTg                >  4:350687/1‑136 (MQ=255)
                                                                                                                    ccGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGCCTCTGGGGCTGTTCCTCGGCATCCTCCTGGCTTACGGGCGCCTGTACCTGGAAAGCGAGATGACCGTGCTGTCgg            <  3:350687/136‑1 (MQ=255)
                                                                                                                    ccGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGCCTCTGGGGCTGTTCCTCGGCATCCTCCTGGCTTACGGGCGCCTGTACCTGGAAAGCGAGATGACCGTGCTGTCgg            <  3:140179/136‑1 (MQ=255)
                                                                                                                          gtgtTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGCCTCTGGGGCTGTTCCTCGGCATCCTCCTGGCTTACGGGCGCCTGTACCTGGAAAGCGAGATGACCGTGCTGTCGGCCACTg      <  3:101574/136‑1 (MQ=255)
                                                                                                                                ttCCTGATAATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTTATTCAGCCTCAGGGGCTGTTCCAAGGCAAACACCTGGCTTAAGGGCGCCTGTACCAGGAAACCGAGATGACCCTGCTGTCGGCCCCAGGCAt  >  2:261283/1‑134 (MQ=255)
                                                                                                                                  |                                                                                                                                   
ATCTGTCCCGCGAGGTCCTGGTCACCTTGAGCGCCGTCAGCGCTGTGCTGTTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGCCTCTGGGGCTGTTCCTCGGCATCCTCCTGGCTTACGGGCGCCTGTACCTGGAAAGCGAGATGACCGTGCTGTCGGCCACTGGCAT  >  ACB143/1113628‑1113889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑