| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 1,188,694 | 0 | T | G | 56.5% | ‑3.1 / 10.8 | 26 | L43R (CTG→CGG) | CJ019_01117 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (2/8); new base G (11/2); total (16/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.43e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.64e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCTCGTGATGCTCACCTCGCGTCTGCTCACCGGTGCTGGCCTGACCCTGGCCGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGGCCACGGCGCCTGCGGTGG‑CCGACAGCCCGCTGCCCGGGCTGATGGTTGGTCACTGGCAGGCGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCTCGGCGGCCTCAAGGCCCAGCCATTGGCCGCCAGTGTGGTG > ACB143/1188559‑1188815 | gCCTCGTGAGGCGCACCTCGCGTCTGCGCACCGGTGCTGGCCTGACCCTGGCCGGCTGGCGTTCTGCGCAGTGGGTGCTGCAGTTGAGCCGGCAGCCACACCCGGCCACGGCGCCGGCGGGGG‑CCGACAGCCCGCt < 5:256197/136‑1 (MQ=255) tGATGCTCACCTCGCGTCTGCTCACCGGTGCTGGCCTGACCCTGGCCGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGGCCACGGCGCCTGCGGTGG‑CCGACAGCCCGCTGCCCgg < 5:64058/136‑1 (MQ=255) gATGCTCACCTCGAGTATTATAACAGGTGCTGGCCTGACCCTGGCCGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGGCCCCGGCGCCTGCGGTGG‑CCGACACCCCGCGGCCCggg > 3:246573/1‑136 (MQ=255) aTGCTCACCTCGCGTCTGCTCACCGGTGCTGGCCTGACCCTGGCCGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGGCCACGGCGCCTGCGGTGG‑CCGACCGCCCCCGGCCCgcgc > 3:193964/1‑136 (MQ=255) cgcgTCTGCTCACCGGTGCTGGCCTGACCCTGGCCGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGGCCACGGCGCCTGCGGTGG‑CCGACAGCCCGCGGCCCGGGCGGAtggttgg > 5:13221/1‑136 (MQ=255) gcgTCTGCTCACCGGTGCTGGCCTGACCCTGGCCGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGGCCACGGCGCCTGCGGTGG‑CCGACCTCCCCCGGCCCCGGCGGAtggtttgt > 8:24205/1‑136 (MQ=255) gcgTCTGCTCACCGGTGCTGGCCTGACCCTGGCCGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGGCCACGGCGCCTGCGGTGG‑CCGACACCCCGCGGCCCGGGCGGAtggttggt > 2:112627/1‑136 (MQ=255) tCTGCTCACCGGTGCTGGCCTGACCCTGGCCGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGGCCACGGCGCCTGCGGTGG‑CCGAGCACCCGCCGCACGGGCAGAagggtggttga > 8:302492/1‑133 (MQ=255) cTGCTCACCGGTGCTGGCCTGACCCTGGCCGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGGCCACGGCGCCTGCGGTGG‑CCGACCCCCCCCGGCCCGGGCGGGtggtgggtga > 3:311194/1‑132 (MQ=255) tCACCGGTGCTGGCCTGACCCTGGCCGGCTGGCTTTCTGCGCACTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGGCCACGGCGCCTGCGGTGG‑CCGACCGACCCCAGCCCCCGCTGATGGTTGGTCACAGGCa > 1:202224/1‑136 (MQ=255) gCCTGACCCTGGCCGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGGCCACGGCGCCTGCGGTGG‑CCGACCGCCCGCGGCCCGGGCTGCTGGTTGGTCACGGGCAGGCGCCCCCcgc > 7:217231/1‑135 (MQ=255) gCCTGACCCTGGCCGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGGCCACGGCGCCTGCGGTGG‑CCGACAGCCCGCAGCCCGGGCTGATGGTTGGGCACTGGCCGGCGcccccccc > 2:284804/1‑134 (MQ=255) gCCTGACCCTGGCCGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGGCCACGGCGCCTGCGGTGG‑CCGACACCCCCCGGCCCCGGCAGCAGGGTGGGCACTGGCCGGCGcccccccc > 1:122809/1‑134 (MQ=255) tGACCCTGGCCGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGGCCACGGCGCCTGCGGTGG‑CCGACAGCCCGCGGCCCGGGCGGATGGGTGGTGCCTGGCCCGCGccccccccccc > 1:263148/1‑131 (MQ=255) cGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGGCCACGGCGCCTGCGGTGG‑CCGACAGCCCGCTGCCCGGGCGGATGGTTGGTGACTGGCCGGCGCCGCCCGCCCCCGGCGcccga > 7:269180/1‑133 (MQ=255) cGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGGCCACGGCGCCTGCGGTGG‑CCGACAGCCCGCGGCCCGGGCTGATGGTTGGTCACTGGCAGGCGCCCCCCCCCCCCGGCGccct > 1:282836/1‑133 (MQ=255) cGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGGCCACGGCGCCTGCGGTGG‑CCGACAGCCCGCGGCCCGGGCGGATGGGTGGGGACTGGCCGGGGCCCCCCCCCCCCGGCGcccg > 6:112791/1‑133 (MQ=255) tgTTGCGCCGGCAGCCACCCCCGCCCACGGCGCCGGCGGGGG‑CCGACAGCCCGCTGCCCGGGCTGATGGTTGGTCACTGGCCGGCGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCt < 1:55013/135‑1 (MQ=255) gTTGTGCCGGCAGCAACACCGGGCCAGGGCGCCGGGGGTGG‑CGGACAGCCCGCTGCCCGGGGTGATGGTTGGTCACTGGCTGGGGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCt < 5:259402/135‑1 (MQ=255) ccGGCAGCCACACCCGGCCACGGCGCCTGCGGTGG‑CCGACAGCCCGCTGCCCGGGCTGATGGTTGGTCACTGGCAGGCGCCCCCCCCCCCCGGGGCCCTCACCATCCAGCGTCTGCCGCAGGCATACCAAggcggc > 4:278139/1‑136 (MQ=255) ccGGCACCCAACCCCGGCC‑CGGCCCCCGGGGGGGCCCTACACCCCGCTGCCCGGGCTGAGGGTTGGTCAGGGGCCGGGGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCTCtgcggc < 1:116764/136‑1 (MQ=255) gCCACACCGGGCCCCGGCGCGGGGGGGGG‑CCGACCGCCCGCTGCCGGGGGTGCGGGTTGGGCCGGGGCTGGGGCGGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCTCGGCGGCCTCAAg < 7:122715/136‑1 (MQ=255) ccccGGCGCGTGGGGGGG‑CCGACAGCCCGCGGCCCGGGCTGAGGGTGGGGCACTGCCCGGCGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCTCGGCGGCCTCAAGGCCCAGCCAtt < 6:96198/133‑1 (MQ=255) gggCGCCGGGGGTGG‑CCGACAGCCCGCTGCCCGGGCTGAGGGTGGGTCACTGGCCGGCGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCTCGGCGGCCTCAAGGCCCAGCCATTGgc < 2:295050/135‑1 (MQ=255) gCGGGGG‑CCGACAGCCCGCTGCCCGGGCTGATGGTGGGTCACTGGCAGGCGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCTCGGCGGCCTCAAGGCCCAGCCATTGGCCGCCAgtg < 7:323707/136‑1 (MQ=255) gg‑CCGACAGCCCGCTGCCCGGGTTTTTGTTTGGTCCCTGCCAGGCGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCTCGGCGGCCTCAAGGCCCAGCCATTGGCCGCCAGTgtggtg < 1:112627/136‑1 (MQ=255) gg‑CCCACCGCCCGCGCCCCGGGCTTATGGTTGGCCACTGCCCGGCGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCTCGGCGGCCTCAAGGCCCAGCCATTGGCCGCCAGTgtggtg < 5:317743/136‑1 (MQ=255) | GCCTCGTGATGCTCACCTCGCGTCTGCTCACCGGTGCTGGCCTGACCCTGGCCGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGGCCACGGCGCCTGCGGTGG‑CCGACAGCCCGCTGCCCGGGCTGATGGTTGGTCACTGGCAGGCGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCTCGGCGGCCTCAAGGCCCAGCCATTGGCCGCCAGTGTGGTG > ACB143/1188559‑1188815 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |