| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 1,537,460 | 0 | T | G | 56.2% | ‑2.7 / 11.8 | 16 | V403G (GTG→GGG) | mdfA | Multidrug transporter MdfA |
| Reads supporting (aligned to +/- strand): ref base T (5/2); new base G (0/9); total (5/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.70e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCCTGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTA > ACB143/1537332‑1537564 | cccGATTGCTTTGGGGGGTTTTGGGGGTGGGGTGCGGGGTTTTATTGGCCCGGGTCCCAGCTTGCTGATTTTTGCCCGGGGGGGGGGGGTGGCGGGGGCGTTGGCGCGGTGGCCGCTGTGGTTTGTggtggg < 6:206956/130‑1 (MQ=255) aCGTTTGCATGGCTGGGGTTGGGGGGAGGGCGTGTGGGTATTATTGGCGCGGGTGCCAGGTTGATGATTTTTGCCCTGGGGGGGGGGGTGGGGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGggc < 2:95345/136‑1 (MQ=255) aCGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCCTGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGggc > 4:88664/1‑136 (MQ=255) ttGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCCTGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTggt > 5:225573/1‑134 (MQ=255) tGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCCTGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTg > 7:86707/1‑134 (MQ=255) ctgGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCCTGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCgg > 3:205180/1‑136 (MQ=255) gtgcATTTTGCCGGGCGGGGGGGGGGGGGGGGGGGGTGGGCGCGGTGGCGGCGGTGGTTGGGGGGGGGGGGGCGGCCGGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTaa < 4:24234/135‑1 (MQ=255) ctttttGCCGGGGGGGGGGGGGGGGGGGGGGGTTTGGCGCTGTGGCGGGTGTGGTTTGTGGGGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAAc < 8:50848/133‑1 (MQ=255) ggggggggggggggggGTGGCGCGGGGGCGGCGGGGGTTGGGGGGGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCGCTTCGCGGGTAAACCCGCTCCTACACAGGgcc < 3:92498/136‑1 (MQ=25) ggggggggggggggCGTGGGCGCGGGGCCCGGGGGGGTTGGGGGGGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGgcc < 5:293810/136‑1 (MQ=25) ggggggggggTGGGGGTGGGCGGTGGGGCGGCTGTGTTTTGGGGGGGGTGGGCGGCTTGGGGAAGGTGCTGTAGCTCGATAATCTGTATTGCCGGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGgcc < 1:60409/136‑1 (MQ=21) ggggggggggggggGTGGGCGCGGGGGCCGCTGGGGTTTGGGGGGGGGGGGCGGCCGGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGgccg < 7:17060/136‑1 (MQ=37) ggggggggggggGTGGGCGCGGGGGCCGCGGGGGTTTGGGGGGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGgccgcg < 1:374107/130‑1 (MQ=37) ggggtgggCGCGGGGGCCGCGGGGGTTTGTGGGGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGt < 1:249251/130‑1 (MQ=38) gCGTTGGCGCGGGGGCCGCGGTGGTTTGGGGGGGGGGGGCGGCCGGGGGACGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTa < 8:294264/136‑1 (MQ=38) cgcTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTaa < 2:10030/96‑1 (MQ=255) cgcTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTaa > 1:10030/1‑96 (MQ=255) | CACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCCTGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTA > ACB143/1537332‑1537564 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |