Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,440,3310TG56.2% ‑1.5 / 16.1 35A19A (GCT→GCG) CJ019_03266hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (14/0);  new base G (10/8);  total (27/8)
Fisher's exact test for biased strand distribution p-value = 4.45e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.72e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGG  >  ACB143/3440210‑3440451
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aaTGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCCCAGGCGGCGCCCGGGc                                                                                                            >  4:291740/1‑136 (MQ=255)
                gTCGGGAGGAGCCCAAGCCCCTCTACAAGCGCGCGCTCGCGGGGCTGCAGTGCGCGCGGGGGCCACCCGCCGGGGCGCCGGGGCGCTGCTGCGGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAg                                                                                            <  8:225249/136‑1 (MQ=255)
                  cTGGAGCACCGCAAGGCCCTCAACCAGCGAGCGCTCGAGGGGCTGCAGGGAGCGCTGGGCCCACCCGCCTGGGCGCCGGGGCGCTGCGGCGGGCCGCCAGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGc                                                                                          <  7:234918/136‑1 (MQ=255)
                             cAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCGGGGTGTGGCAGGCCAACGCCtat                                                                                >  3:144019/1‑135 (MQ=255)
                             cAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCGGGCGGCGCCCGGGCAGCCGGGGTGTGGCAGGCCAACGCCgat                                                                                >  8:181311/1‑135 (MQ=255)
                              aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTTAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCCGCCCAGCCGGCCCCCCGGGAGCCGGGGGGTGTCAAGCCAAcccccaaa                                                                               >  6:6101/1‑131 (MQ=255)
                              aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCTGGGTGTGGCAGGCCACCGCCgata                                                                               >  3:320126/1‑135 (MQ=255)
                              aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGCGGGCCAACGCCgata                                                                               >  1:268461/1‑135 (MQ=255)
                              aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGGGGCGGCCGGGCCGCCTGGGTGTGTGAGGACCACCGCTATaa                                                                              >  6:39985/1‑136 (MQ=255)
                                ggCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCTGGCGGCGCCCGGGCCGCCTCGGTGTGGGAGGCCAACGCCGATAAgg                                                                            >  2:78158/1‑136 (MQ=255)
                                        aCAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCGGGCCGCCGGGGGGTGGCAGGCCCCCCCCGATAAGGCGCAGcgc                                                                    >  6:1827/1‑134 (MQ=255)
                                          aaGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCTGGCGGCGCCCCGGCCGCCGGGGTGTGTCCGTCCAACGCCAATTAAGGGCAGCTCGc                                                                  >  8:107399/1‑136 (MQ=255)
                                               agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCCCCCAGGCGGCGCCCGCGCAGCCGGGGGGGCGGACGCCAACCACAATAAGGAGCCACTCGCTGAt                                                              >  4:164162/1‑135 (MQ=255)
                                               agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGCCGGCCCACGCCTATAAGGCGCAGCTCGCTGAt                                                              >  7:217716/1‑135 (MQ=255)
                                               agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCACCGCGGGCGGCGCCCCGGCCCCCAGGGGGGGGAAGGCCCACCACCATAACGCGCACCTCGCGGATc                                                             >  8:156415/1‑136 (MQ=255)
                                                  gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCCACGCCAATAAGGCGCCGCGCGCGGATCAgg                                                          >  1:228212/1‑136 (MQ=255)
                                                  gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGGCGCCGGGGTGTGGGCGGCCCACCCCGATAATGCGCCGCTCGCGGATCAgg                                                          >  4:144743/1‑136 (MQ=255)
                                                  gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCGGGCAGCCTGGGGGTGGGAGGCCCACGCCTATAAGGGGCAGCGCGCTGATCAgg                                                          >  1:143639/1‑136 (MQ=255)
                                                  gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCCGGCAGCCGGGGTGTGGCCGGCCAACCCCTATAAGGCGCCGCGCGCTGCTAAgg                                                          >  8:288313/1‑136 (MQ=255)
                                                    tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCCACGCCAATAAGGCGCCGCTCGCTGATCAGGcc                                                        >  7:11022/1‑136 (MQ=255)
                                                                 gTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCCCGCCCCCAGGGGGTGGCAGGCCACCCCCGATAAGGCGCAGCGCGCTGATCAGGCCGAAGACATCGGc                                            >  7:342671/1‑135 (MQ=255)
                                                                     gcgcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGCAGGCCCCCGCCGATAAGGCGCCGCTCGCTGCTCAGGCCGATGACTTCGGCAAg                                         >  8:41740/1‑134 (MQ=255)
                                                                       gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCTGGGTGTGGCCGGCCCACGCCGATAAGGCGCCGCTCGCTGATCAGGCCGAGGACGTCGGCAAg                                         >  3:323969/1‑132 (MQ=255)
                                                                       gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCTGGCGGCGCCCGGGCCGCCGGGGGGGGGGAGGCCCACGCCGATAAGGCGCCGCGCGCTGGTCAGGCCGCTGAGGTCGGCAAGCCGt                                     >  6:322311/1‑136 (MQ=255)
                                                                       gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCCGCCGGGGGGTGGGAGGCCAACGCCGATAAGGCGCAGCTCGCTGATCAGGCCGATGACGTCGGCAAGCAGt                                     >  3:154996/1‑136 (MQ=255)
                                                                                    gCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGGCGCCTGGGGGTGGGCGGCCCACGCCTATAAGGCGCCGCTCGCGGATAATGCCGATGACTTCGGCAAGCAGTTGGGGGAGAAGGa                        >  8:11333/1‑136 (MQ=255)
                                                                                    gCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCGGGGTGTGGGAGGGCAAAGCCTATAAGGCGCAGCTCGCGGAGCAGGGCGAGGACGTCGGCAAGCAGTTGGCGGAGAAggg                        >  1:231926/1‑135 (MQ=255)
                                                                                    gCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCGGGGGGTGGGCGGCCCCCCCCGATAAGGCGCCGGGCGCGGGTCAGGCCGCTGCGGTCGGCCAGCCGTTTGCGGGGACggg                        >  5:3763/1‑135 (MQ=255)
                                                                                       gggTCGGGGTGGCGCGGCGGCTGGCCGCCAGCGGGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcg                     <  8:12776/135‑1 (MQ=255)
                                                                                          tCGCCGGGGGGCTGCGGCGGCCCGCCCGGGGGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc                  <  5:69608/136‑1 (MQ=255)
                                                                                          gggcggTGGCGGGGCGGCTGGCCGCCCGCGCGGCCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc                  <  3:121928/131‑1 (MQ=255)
                                                                                          gcggggTGGCGCTGCGGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc                  <  3:166815/131‑1 (MQ=255)
                                                                                          gcccggTGGCGCGGCGGCGGGCGCCCCGCGCGGGCCGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc                  <  7:181364/131‑1 (MQ=255)
                                                                                                 ggCGCTGCTGCTGGGCGCCAGCGCGCGGGGCCCCCGGGCAGCCGGGGTGTGGCAGGCCCCCCCCCAGAACGCGCCGCCCGGGGATCAGGCCGGGGACTACGGCGAGACGATGGCGGAGAAGGATCGCGCGCACggg           >  8:49333/1‑135 (MQ=255)
                                                                                                          gcgggCCGCCCGCGGGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCgg  <  1:325521/133‑1 (MQ=255)
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AATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGG  >  ACB143/3440210‑3440451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑