Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,245,1280AC40.7% 28.4 / 10.0 60L178R (CTG→CGG) CJ019_03982Crotonyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base A (10/22);  new base C (20/2);  total (30/30)
Fisher's exact test for biased strand distribution p-value = 1.60e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCGGCC  >  ACB143/4245002‑4245256
                                                                                                                              |                                                                                                                                
tCGCGTAGCAGCCGCTTGGGCTGCGCATAGGCGAAGGGCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGAt                                                                                                                         <  6:3976/136‑1 (MQ=255)
            cGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCcaca                                                                                                             <  6:61171/136‑1 (MQ=255)
                 ggTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACtcg                                                                                                        >  2:230921/1‑136 (MQ=255)
                 ggTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACacg                                                                                                        >  5:187784/1‑136 (MQ=255)
                  gTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACtcgt                                                                                                       <  6:147878/136‑1 (MQ=255)
                        gcATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCCGGCGCGCACGGCGCCCCACCACTCGTCGAgg                                                                                                  >  3:55756/1‑135 (MQ=255)
                          aTAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCACCCCGGCGGCCCGGGCGCGCTACGCGCCCCCCCACGCGTCGCGGacc                                                                                               >  1:371429/1‑134 (MQ=255)
                          aTAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAggca                                                                                                >  1:97485/1‑133 (MQ=255)
                          aTAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGaa                                                                                                >  8:281152/1‑135 (MQ=255)
                           tcggCGCAGGTCGGGCCAGTGTCCCGGCGCCGGGCAAGTTCGGCGGCAACGGCAGGCAACTGCGCGTCGGCGAAGACTTCGCTGACCACCCCGGCGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAAca                                                                                              <  4:62382/134‑1 (MQ=255)
                           tAGGCGACGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAAca                                                                                              <  3:118733/136‑1 (MQ=255)
                           tAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                              <  8:8922/136‑1 (MQ=255)
                            aGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                              <  2:70437/135‑1 (MQ=255)
                            aGGCGAAGGGCGGGCCATTGGCGAGGCGCCCGGCCAGTTCGGCGGCAACGCCCGGCAACTGCTCGTCGTCGCAGACTTCGCTGACAACCCGGGCGGCCTGGGCGCTATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                              <  4:247548/135‑1 (MQ=255)
                               cGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCACCCCGGCGGCCCGGGCGCGCTCGGCGCCCCACCACTCGTCGAGGAAcagcag                                                                                          >  1:260375/1‑136 (MQ=255)
                               cGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCCCAACTCGTCGCGGAAccgcag                                                                                          >  6:177428/1‑136 (MQ=255)
                                gAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGGCCAACCCGGCGGCCAGGGCCCGCACCGCCCCCCACAACACCGCGAGGGACACCAgg                                                                                         >  4:123573/1‑136 (MQ=255)
                                gAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGCCCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCCCAACTCGTCGAGGAACAGCAgg                                                                                         >  2:21133/1‑136 (MQ=255)
                                    gtggggCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCt                                                                                     >  7:343098/4‑136 (MQ=255)
                                    gTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATAGGCGCCCCCCCACTCGGCGAGGAACAGCCGGCGCt                                                                                     >  5:13579/1‑136 (MQ=255)
                                    gTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCCGGGGCGCTCCGCGGCCCCCCACACCTCGAGGAACAACAGgccca                                                                                     >  2:329329/1‑133 (MQ=255)
                                         gCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCCCAACACGTCGAGGAACAGCAGGCGCTTGGc                                                                                 >  4:135908/1‑135 (MQ=255)
                                           cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGGTCGGCGCCCCCCAACGCGTCGAGGAACACCCGGCGCGTGGccgg                                                                              >  1:248179/1‑134 (MQ=255)
                                           cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCACCACTCGTCGCGGAACAGCAGGCCGTTGGCCTg                                                                              >  1:41934/1‑136 (MQ=255)
                                           cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCCCGATAGGCGCCCCACCACACGTGGAGGAACAACCGGCGCGTTGccgg                                                                              >  3:282408/1‑134 (MQ=255)
                                           cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCCGGCCCGATCGGCGCCCCACAACTCCGCGAGGCACACCAGGCCCATGGccag                                                                              >  6:260358/1‑134 (MQ=255)
                                           cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCACGGCGCCCTCGGGGGCCCACCACAAGTCGAGGAACACCAGGCGCGTGGccgg                                                                              >  3:218800/1‑134 (MQ=255)
                                               ggCCTGGCGCCCGGCCAGTTCGGCGGCACCGGCCGGCACCGGCGCGGCGCCGCAGAGTTCGCTGACCAACGCGGCGGCCTGGGGGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCg                                                                          <  7:247373/136‑1 (MQ=255)
                                                gCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCACAACTCGTCGAGGCACAACCGGCGCGTGGCCAGGTCGc                                                                         >  4:70839/1‑136 (MQ=255)
                                                        cccGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCACCACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGAt                                                                  >  5:308293/1‑135 (MQ=255)
                                                        cccGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCACCACGCGGGGAGGCACCGCCGGCGCGTTGCCGGCGCGCGGCCgct                                                                  >  4:195267/1‑133 (MQ=255)
                                                              ctgTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGt                                                           <  6:334619/134‑1 (MQ=255)
                                                                    ggcggcAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCCGGCGCGATCGGCGCCCCCCAACTCGGCGAGGCACACCCGGCGCATGGCCGGCACGCGGCCCCGCAGCCGGGGCacg                                                     >  6:213066/1‑134 (MQ=255)
                                                                              gcaggcaACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCCGGCGCGATCGGCGCCCCCCAACTCGTCGAGGCACACCCGGCGCTTGGCCGGCTCGCTGCCGATAAGCCGTGGCCGGTGCCAGCAgg                                           >  1:150499/1‑136 (MQ=255)
                                                                              gcaggcaACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCCGCGGCCAGGGCGCGATCGGCGCCCCACAACTAGTAGAGGCACAGCAGGCGCTTGGCCAGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTgg                                           >  8:79896/1‑136 (MQ=255)
                                                                               ctggcaACTGCTCGTCGGCGCAGCCTCCGCTCCCCACCCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGgc                                          <  3:130706/134‑1 (MQ=255)
                                                                                      cTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                                   <  8:205232/136‑1 (MQ=255)
                                                                                      cTGCTCGTCGGCGAACACTTCTCTTACCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                                   <  8:347406/136‑1 (MQ=255)
                                                                                      cTGCTCGGCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                                   <  5:148399/136‑1 (MQ=255)
                                                                                      cTGCTCGGCGGCGCAGACTTCGCTCACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                                   <  1:80104/136‑1 (MQ=255)
                                                                                      cTGCGCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                                   <  4:36365/136‑1 (MQ=255)
                                                                                         cgcggcgGCGCAGACTTCGCTGACCACCCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCAt                                  <  3:115063/129‑1 (MQ=255)
                                                                                          tcgtcgGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGAt                                                                                                                         >  2:251639/1‑46 (MQ=255)
                                                                                          tcgtcgGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGAt                                                                                                                         <  1:251639/46‑1 (MQ=255)
                                                                                           cggcgGCGCTCACTCCGCGCACCACCCCGGCGGCCTGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCggg                              <  6:16336/133‑1 (MQ=255)
                                                                                           cggcgGCGCAGACTTCGCGGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCggg                              <  5:275489/133‑1 (MQ=255)
                                                                                            gccgGCGCAGACTGCGCTGACCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCgggg                             <  6:334917/134‑1 (MQ=255)
                                                                                                gcgcAGACTTCGCTGACCACCCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGt                         <  6:128174/136‑1 (MQ=255)
                                                                                                gcgcAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACATCTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGt                         <  1:38855/136‑1 (MQ=255)
                                                                                                gcgcAGACTTCGCGCCCCAACCCGGCGGCCTGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGt                         <  2:268135/136‑1 (MQ=255)
                                                                                                ccgcAGACTGCGCTCACCACCCCGGCGGCCCGGGCTCTAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAACCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGt                         <  3:344111/135‑1 (MQ=255)
                                                                                                 cgcAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTg                          >  4:113954/1‑134 (MQ=255)
                                                                                                 cgcAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTa                        <  7:227760/136‑1 (MQ=255)
                                                                                                               accaccCCGGCGGCCTGGGCGCGTTCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt          <  2:61174/136‑1 (MQ=255)
                                                                                                               aCCAACCCGGCGGCCTGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt          <  7:109089/136‑1 (MQ=255)
                                                                                                               aCCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt          <  3:130182/136‑1 (MQ=255)
                                                                                                               aCCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt          <  6:85858/136‑1 (MQ=255)
                                                                                                                   aCCCGGCGGCCCGGGCGCGATCGGCGCCCCCCAACTCGTCGAGGAACAGCCGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCAACCGGGCTGTAGCCCCTGCTGGTGTAGcc      >  7:125469/1‑136 (MQ=255)
                                                                                                                   aCCCGGCGGCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCACGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGcc      >  5:93223/1‑136 (MQ=255)
                                                                                                                       ggcggcCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCCGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCGGGTGTAGCCGGcc  >  6:118988/1‑136 (MQ=255)
                                                                                                                              |                                                                                                                                
TCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCGGCC  >  ACB143/4245002‑4245256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑