Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,927,1850AC52.8% 4.7 / 20.6 36W194G (TGG→GGG) srp54Signal recognition particle 54 kDa protein
Reads supporting (aligned to +/- strand):  ref base A (11/6);  new base C (11/8);  total (22/14)
Fisher's exact test for biased strand distribution p-value = 7.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.77e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CGGCCAGCTTGGCCAGGGTGGTGGTCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTGCAGCTGATTCCAGG  >  ACB143/4927053‑4927319
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cgGCCAGCTTGGCCAGGGTGGTGGTCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGc                                                                                                                                     >  3:122696/1‑136 (MQ=255)
      gCTTGGCCAGGGTGGTGGTCTTGCCCAGGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGgcg                                                                                                                               <  4:273690/136‑1 (MQ=255)
                 gtggtgggCTTGCCCATGCCCGCCGGGCCGACCATGGCTATCACCCCACCCTCTTCGATCGGCTCTATTACAGCCACTTCCATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGa                                                                                                                    <  8:267285/136‑1 (MQ=255)
                    ggggtCTTGCCCAGGCCCGCGGGGCCGACCATGGCGATCCCCCCACCCTCTTCGATCGGCGCTATTTCCGGCACTTCGATCACCCGGCCCACGGCCGCCAGCAGCATGCCCCCGGCCGGGCGCGGCTCTTCGAtct                                                                                                                 <  2:94521/134‑1 (MQ=255)
                         ctgccccACGCCCGCCGGGCCGACCATGGCCACCCCCCCACCCTCTTCGCTCGGCGCGATTCCCGCCACTTCTATCACCCGCCCCAAGCGCCCCAGCAGCCCCCGCCCGCCCGGGCGCGGCTCTTCGATCTcggcg                                                                                                            <  4:123835/132‑1 (MQ=255)
                         cTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCGCTTCGCTCTcggcg                                                                                                            >  6:145251/1‑136 (MQ=255)
                            gCCCAGGCCCGCCGGGCCCACCCGGCCCACCCCCCCACCCTCTGCGCTCGGCTCTATTTCAGCCACTTCGATCCCCCGTCCCAAGGCCCCCCGCCGCAGGCCCCCGGCCGGGCGCGGCTCTTCGATCTcggcg                                                                                                            <  2:195334/133‑1 (MQ=255)
                             cccATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCCCTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCTGGCGCGGGGCGTCGGGCTCGGCCGTcc                                                                                                        >  4:131389/1‑135 (MQ=255)
                             cccATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCa                                                                                                        <  7:122223/136‑1 (MQ=255)
                               cATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCGGGCGCGGCGGTTGGAGGTGGGCGGGCAgg                                                                                                      >  4:164420/1‑136 (MQ=255)
                               cATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCGGGCGCGGCGCGTGGCTCGcggcggggcgg                                                                                                      >  5:214653/1‑131 (MQ=255)
                               cATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGCTCTAGGCGGTCAgg                                                                                                      >  6:101544/1‑136 (MQ=255)
                                    ccgccgGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCGGGCGCGGCTCGTCGAGCTCGGCGGGCAGGTcccg                                                                                                 >  7:215440/1‑134 (MQ=255)
                                     cgccgGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCGGGCGCGGCGCGTCGCTCTCGGCGGGCAGGtgccgc                                                                                                >  7:287409/1‑131 (MQ=255)
                                     cgccgGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCCGCCTGGCGCGGCTGGTCGATCTCGGCGGGCAGGTCccgc                                                                                                >  4:358729/1‑136 (MQ=255)
                                     cgccgGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCGCGTCGATCTCGGCGGGCAggggccgc                                                                                                >  2:74004/1‑130 (MQ=255)
                                          ggCCGACCATGGCGATCCCCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGAGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTATTCGATATCGGCGGTCAGGTCCagcagc                                                                                             >  6:364312/1‑134 (MQ=255)
                                               aCCAGGGCGACCCCCCCACCCGCTTCGAGCGGCGCTATTTCCGCCACTCCGACCAGCCGGCCCAAGTCCCCCAGCCCCAGGCGCCCGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCggg                                                                                      <  6:299275/136‑1 (MQ=255)
                                                    ggCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGAGCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATc                                                                                 <  6:276779/136‑1 (MQ=255)
                                                         tccccccacccTATTCGATCGGCTCGATTTCCGGCCCTGCGATCATCCGCGCCACGTGACCCCCCAGCAGGCGCCCGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGccc                                                                            <  3:248314/133‑1 (MQ=255)
                                                            ccccacccTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCTGGCGCGGCGCGTCGATCTCCGCGGTCAGGGCCAGCCGCTCGCGGGCGATCGGCCCggc                                                                         >  7:324513/1‑135 (MQ=255)
                                                                     ctTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCACGCCTGGCGCGGCTCTTGGCTCTCGGCGGTCCGGTCCAGCCGCTCGCGGGCCATCGGCCCGGCCAGGCCg                                                                  >  1:312659/1‑134 (MQ=255)
                                                                         gATCGGCTCGATTTCAGGCACTTCGATCATCCTTGCCAAGTGAGCCAGCAGCATCCGCCCTGCCTGGCGCGGCTCTTCGATATCGGGCGTCAGGTCCAGCAGGTCCCCTGCGATCCGCACGCACAGGCCGATgcgc                                                            >  8:111566/1‑136 (MQ=255)
                                                                         gATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACCGGCCCATgcgc                                                            >  6:168690/1‑136 (MQ=255)
                                                                         gATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCCGCCGGGCGCGGCTCGTCGATCTCGGCGGGCACGTCCACCCGCGCGCGGGCGCTCGGCCCCGGCAGGCCCATgcgc                                                            >  4:311029/1‑136 (MQ=255)
                                                                          aTCGGCTCGATTTCAGGAACTTCGATCAGCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCGCTTCGATCTCGGCGGGAAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGCCAGTCCGATTCGCt                                                           >  6:106850/1‑136 (MQ=255)
                                                                                   aTTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCCGGTCCAGCCGCTCGCGGGCGATCGGCCCCGACAGGCCGCTGCGCTGCAggcggc                                                  >  5:170599/1‑136 (MQ=255)
                                                                                    tttCCGCCACTTCGATCATCCGTCCCAAGGCCGCCAGCACCAGGCGCCCGGCGGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAggcggc                                                  <  2:40459/135‑1 (MQ=255)
                                                                                           cACTTCGAGCCTCCGTGCCAAGGGAGCCAGCAGCATGCGCCCGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAgg                                          <  7:230347/136‑1 (MQ=255)
                                                                                                cccgcatcCGTCCCACGGGACCCACCAGCATGCGCCACGCGTGCCGCGGCTCTTCGATCTCGGCGGGCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGc                                     <  5:200536/132‑1 (MQ=255)
                                                                                                     atcCGTGCCAAGTGCGCCAGCAGCAGGCGCCCGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCctgct                                <  4:81031/136‑1 (MQ=255)
                                                                                                               aGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTtgct                      >  2:226437/1‑136 (MQ=255)
                                                                                                                  gAGCCAGCAGCATGCGCCAGGCCAGGCGCGGCTCGTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGCCAGGCCGCTGCGCTGCCGGCGGCGCCAAAGGTTGGCCGGCTGCGGCTTGCTGcc                   >  2:274746/1‑136 (MQ=255)
                                                                                                                    gCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGCCCGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCGGCGGCTTGCTGGCCt                 >  1:252984/1‑136 (MQ=255)
                                                                                                                              tGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTGCAGCTGAt        >  3:55465/1‑135 (MQ=255)
                                                                                                                                   cAGGCCTGTCGCGTCTCTTCGATCTCGGCGGGCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGTACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTGCAGCTGATTCCAgg  <  5:65591/136‑1 (MQ=255)
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CGGCCAGCTTGGCCAGGGTGGTGGTCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTGCAGCTGATTCCAGG  >  ACB143/4927053‑4927319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑