Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,693,1830TG42.9% 5.6 / 13.0 42R238R (CGT→CGG) pyrC'Dihydroorotase‑like protein
Reads supporting (aligned to +/- strand):  ref base T (6/18);  new base G (0/18);  total (6/36)
Fisher's exact test for biased strand distribution p-value = 2.92e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCGCCGCTGCG  >  ACB143/5693051‑5693317
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aGGTGCCATGGCCAGCTGCCGCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGGGGCCCTGGCGCGCAACCTGCTGCTGGGGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGgc                                                                                                                                     <  5:351697/136‑1 (MQ=255)
aGGGGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGGGGCCCTGGCGCGCAACCTGCTGCGGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCCCCAGCGCCCGTGgc                                                                                                                                     <  7:194478/136‑1 (MQ=255)
            cAGCTTCCTCGGCCTGCCGGGCTTCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATc                                                                                                                         <  3:252994/136‑1 (MQ=255)
            cAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGCGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATc                                                                                                                         <  6:81054/136‑1 (MQ=255)
                  ccTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATAgccccg                                                                                                                   >  7:206028/1‑134 (MQ=255)
                  ccTCGGCCTGCCGGGAATCCCGCAGAGCGCCGAAACCTTGGCCCTGTCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCACATCACCCGCGCCCGTGGCGCGCGGCTGATCgcccag                                                                                                                   <  3:260347/136‑1 (MQ=255)
                   cTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCgcccagg                                                                                                                  <  5:80939/136‑1 (MQ=255)
                      ggCCTGCCGGGCATCCCGGAGAGCGCGGAAACCGGGGCCCTGGCGCGCACCCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCCGATCACCCGCGCCCGGGGCGCGCGGCTGATCgcccaggc                                                                                                                 <  7:100094/134‑1 (MQ=255)
                      ggCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGGGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCgcccaggc                                                                                                                 <  5:341391/134‑1 (MQ=255)
                        ccggCCGGGCATCCCGGCGCGCGCCGAACCCGTGGCCCGGGCGCGCAACCTGCGGCTGGTGGACCAGCGCGGCGGGGGTGCGCAGTGCCGCCAGATCCCCCGCGCCCGTGGCGCGCGGCTGATCgcccaggcccag                                                                                                             <  2:253104/133‑1 (MQ=255)
                              gggCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTg                                                                                                       <  5:178056/136‑1 (MQ=255)
                                    cccGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCCCGTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCGGCTAGCCCCCGCCCCCGCCGTGGGccgg                                                                                                 >  2:130606/1‑134 (MQ=255)
                                            gcgcCTAACCGGGGGCCCGGGCGCGCAACCTGCGGCTGGGGGAACTGCGCGGGGGGGGGGCGCACTTCAGCCAGAACACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                                         <  3:220096/136‑1 (MQ=255)
                                            gcgcCTAAACGGTGGCCCGGGCGCGCAACCTGCTGGTGGTGTAACAGAGCGGCGTGGGTGCGCACTTCAGCCACATCACCAGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                                         <  6:248283/136‑1 (MQ=255)
                                            gcgcCGAAACCGTGGCCCTGGCGCGCAACCTGCGGCTGGTGGAACAGAGCGGCGTGGGGGCGAACTTCAGCCAGATCACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                                         <  8:283656/136‑1 (MQ=255)
                                                cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGCTCGCCCAGGCCCCGGCCGTGGGCCTGCCGGTAAccgcc                                                                                     >  2:89831/1‑136 (MQ=255)
                                                 gaacgggTGGCCCTGGCGCGCAACGTGCTGCGGGGGGAACAGAGGGGCGGGGGTGCGCACTGCCGCCAGATCACCTGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                                                    <  1:90844/130‑1 (MQ=255)
                                                 gAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGGGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAActgccg                                                                                    <  3:86358/136‑1 (MQ=255)
                                                  aaaCCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCACATAACCACCCCCCCTGGCGCGCGGCTGATCCCCCCCGCCCCGGCCGTGGGCCTGCCCGTAAcccccc                                                                                    >  7:47623/1‑134 (MQ=255)
                                                      gtgggCCCTGCCGGGAAACCGTCTGGTGGTGTAACTGTGGGGGGGGGGTGGGAACTTCCGCCAGATACCCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                               <  8:126076/133‑1 (MQ=255)
                                                      gggggCCCTGGCGCGCAACCTGCGGCGGGGGGAACAGCGGGGCGGGCGAGCGCACTTCCGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                               <  3:225615/133‑1 (MQ=255)
                                                      gggggCCCGGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGGGTGCGTGCGCAATTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                               <  7:114385/133‑1 (MQ=255)
                                                      ctgggCCCGGGGGGGCACCCTGGTGTTGGTGGAAGAGGGGGGGGGGGGTGCGCTCTGCCGCCCGTTCACCGGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                               <  6:278589/133‑1 (MQ=255)
                                                      cggggCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGGGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                               <  4:27315/133‑1 (MQ=255)
                                                      cGTGGCCGGGGCGCGAAACCTGCGGCTGGGGGAACAGAGGGGCGGGCGGGCGCAGTCCAGCCAGAACACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                               <  2:83657/136‑1 (MQ=255)
                                                      cGTGGCCCTGGCGCGCAACGTGCTGCGGGTGGAACAGAGGGGCGTGGGTGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                               <  5:77337/136‑1 (MQ=255)
                                                      cGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                               <  5:270119/136‑1 (MQ=255)
                                                       gTGGCCGTGGCGGGCAACCTGCTGCGGGTGGAACAGAGCGGGGTGGGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAg                                                                              <  2:49803/136‑1 (MQ=255)
                                                          ccccGGGCGCGCACCCTGCGGCGGGGGCAACAGCGCGGCGGGCGTGCGAACTCCCCCCTCACCCCCCGCGCCCGGGGCCCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAgcgc                                                                           <  6:218142/135‑1 (MQ=255)
                                                                    gcAACCTGCTGCTGGGGGAACAGAGCGGCGGGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTTCATGTAACCGCCGATGTAGCGCTGTACCAGCt                                                                 <  2:43973/136‑1 (MQ=255)
                                                                          tgctggtgGTGTAACAGAGCGGCGGGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCt                                                           <  2:30003/136‑1 (MQ=255)
                                                                                            gcggcgTGCGGGCGCACTCCAGCCCGACCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg                                         <  7:43198/136‑1 (MQ=255)
                                                                                                 gtgcgggcGCACTTCCGCCAGATCACCCGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                                    <  2:113115/136‑1 (MQ=255)
                                                                                                 gtgcgggcGCACTTCAGCCAGAACACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                                    <  5:79879/136‑1 (MQ=255)
                                                                                                 gggcgtgcgAAGTGCCGCCTCATCCCCAGGGCCCTGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                                    <  2:335234/134‑1 (MQ=255)
                                                                                                 gggcgggcgCACTTCCCCCAGATCACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                                    <  1:166455/134‑1 (MQ=255)
                                                                                                        cgcACTCCAGCCAGATCCCCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAg                             <  4:264642/136‑1 (MQ=255)
                                                                                                                        cACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAg             <  6:118908/136‑1 (MQ=255)
                                                                                                                        cACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTGGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGAGCGAGTTCTCCAGCCTGTACCACGTGCAg             >  4:202231/1‑136 (MQ=255)
                                                                                                                         ccccccgcgcgGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCCCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAgc            <  6:235157/131‑1 (MQ=255)
                                                                                                                                cccGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCGCCGCt     <  6:338895/136‑1 (MQ=255)
                                                                                                                                   gTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCGCCGCTgcg  >  7:218861/1‑136 (MQ=255)
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AGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCGCCGCTGCG  >  ACB143/5693051‑5693317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑