Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1436,043,1960CA33.7% 89.4 / 36.5 89A37S (GCC→TCC) puuD_3Gamma‑glutamyl‑gamma‑aminobutyrate hydrolasePuuD
Reads supporting (aligned to +/- strand):  ref base C (27/32);  new base A (0/30);  total (27/62)
Fisher's exact test for biased strand distribution p-value = 1.23e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGGGGGCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTAC  >  ACB143/6043064‑6043327
                                                                                                                                    |                                                                                                                                    
tGGGGGCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCaaa                                                                                                                                   >  6:239343/1‑136 (MQ=255)
     gcgcCATTGTAATGATGCTGCTCGATATTTGAAGGCGAGCCGTTAAAAACAAAGCAGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCTCAATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑gcagg                                                                                                                             >  6:293506/1‑136 (MQ=255)
        ccATTGAAATGATGCGGCTCTATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCCGCGCTTGCGGGGCGCTGCCGCCATCACGGGCCGGAAAGATCAGTGCCAAGCCGCAAAA‑gccggccg                                                                                                                          <  8:67267/136‑1 (MQ=255)
           ttGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCt                                                                                                                       >  3:340542/1‑136 (MQ=255)
           ttGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCt                                                                                                                       >  6:298261/1‑136 (MQ=255)
               aaTGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGa                                                                                                                    >  8:335433/1‑135 (MQ=255)
                      cGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTg                                                                                                            >  4:237994/1‑136 (MQ=255)
                      cGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTg                                                                                                            >  1:302653/1‑136 (MQ=255)
                      cGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTg                                                                                                            >  3:266178/1‑136 (MQ=255)
                       ggCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGt                                                                                                           >  3:363794/1‑136 (MQ=255)
                         cTCGATATTTGAAGGCGCGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCCAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGCCg                                                                                                         >  6:68050/1‑136 (MQ=255)
                         cTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCg                                                                                                         >  6:32542/1‑136 (MQ=255)
                         cTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCg                                                                                                         >  1:275753/1‑136 (MQ=255)
                          tCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGc                                                                                                        >  5:351067/1‑136 (MQ=255)
                            gATATTTGACGGCGAGCCGGTAAAAACAATGCCGGGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCAATCACGGGCCGGCAAGAGCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGcc                                                                                                       <  2:35321/135‑1 (MQ=255)
                              tttttGACGGCGAGCGGTTAAAAACACTGCCGGGCCGGCGAGCCAGCCGCGCTGGGGGGGCGCGGCCGCAATCCGGGGCCGGCAAGATCCGGGGCACCCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGcc                                                                                                       <  6:351067/131‑1 (MQ=255)
                              tatTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGGCGCGGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGcc                                                                                                       <  7:342529/133‑1 (MQ=255)
                                    acggCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCgtgt                                                                                               <  7:230571/133‑1 (MQ=255)
                                        cgcgCCGGTAAAAACAATGCGGTGCAGGCTAGCCAGCAGCGCTTGGGTGTCGCGGCCGCCATCACGGGCCGGCAAGATCAGTGGCAACCCGGCAAA‑GCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCgag                                                                                          <  5:348451/133‑1 (MQ=255)
                                           gCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCgaggag                                                                                       >  1:106442/1‑136 (MQ=255)
                                            ccGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGt                                                                                      >  3:332880/1‑136 (MQ=255)
                                             cGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGtt                                                                                     >  1:32442/1‑136 (MQ=255)
                                                tAAAAACATGGCCGTGCAGGCTCGCCGCCCGGGGTGGGGGGGCGGTCCCGCAATCCCGGGCCGCAAAGATCCGT‑GCCACCCGGCAAACGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGttctt                                                                                  <  6:188853/136‑1 (MQ=255)
                                                            cGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGCCCGTGTGCGAGGAGTTCTTCCCTACcagct                                                                       >  1:151050/1‑135 (MQ=255)
                                                            cGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACctgct                                                                       >  5:218493/1‑135 (MQ=255)
                                                               gccggCGAGCCAGCAGCGCTGGCGGGGCGGGGCCGCCATCCCGGGCCGGAAAGATCTGGGCCACGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg                                                                   <  6:302595/133‑1 (MQ=255)
                                                               gCAGGCTAGCCCGCCGCGGTGGGGTGGCGCGCCCGCCATCCCGGGCGGCAAAGATCAGTGCCACCCCGCAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg                                                                   <  6:231675/136‑1 (MQ=255)
                                                                 aGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACgg                                                                  >  3:188657/1‑135 (MQ=255)
                                                                 aGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGGTCTTCCCTACCTGCTGACGGc                                                                 >  5:48442/1‑136 (MQ=255)
                                                                         cagctgCGCTTGCGTGGCGCTGCCGCCATCACGGGCCGGCAAGATCTGTGCCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTg                                                         <  7:201425/136‑1 (MQ=255)
                                                                         cagcagCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCt                                                          >  5:302595/1‑135 (MQ=255)
                                                                          agccgcGCTGGCGTTGCGCTGCCGCCATCACGGGCCGCCAAGATCAGGGGCACCCCGGAAAC‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGa                                                        <  3:358292/136‑1 (MQ=255)
                                                                            cagcGCTTGGGTGTCGCTGCCGCAATCACGGGCCGGCAAGATCAGTGGAAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGaca                                                      <  8:6473/136‑1 (MQ=255)
                                                                                    gCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCa                                               >  8:349847/1‑135 (MQ=255)
                                                                                    gCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCa                                               >  8:99599/1‑135 (MQ=255)
                                                                                    gCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCa                                               >  2:33744/1‑135 (MQ=255)
                                                                                       tgtCGCTGCCGCCATCAGGGGCGGGCAATATCAGTGGCAAGCCGGCAAT‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAgg                                           <  1:271914/136‑1 (MQ=255)
                                                                                                     tccggggCCGGCAACATCCGTGCCAAGCCGCCAAT‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGGTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGGATTTgcgc                             <  5:315779/132‑1 (MQ=255)
                                                                                                     tccggggCCGGAAATATCTGTGCCACG‑CGGCAAACGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  3:237994/132‑1 (MQ=255)
                                                                                                     tcccgggcgggCAAGACCCGTGCCACGCCGTCAAA‑CCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  3:276882/133‑1 (MQ=255)
                                                                                                     tcccGGGCCGGCACGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  5:119771/133‑1 (MQ=255)
                                                                                                     tcccGGGCCGGCAATTTCAGGGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  8:345152/133‑1 (MQ=255)
                                                                                                     tCAGGGGCCGGAATTATCTGGGGCATCCCGCAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  3:296604/136‑1 (MQ=255)
                                                                                                     tCAGGGGCCGGAAAGATCAGGGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  3:128663/136‑1 (MQ=255)
                                                                                                     tCACGTGCCGGCATGATCTGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  3:273790/136‑1 (MQ=255)
                                                                                                     tCACGGGCCGGCAATATCAGTGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  5:362468/136‑1 (MQ=255)
                                                                                                     tCACGGGCCGGCAATATCAGTGCCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  8:30185/136‑1 (MQ=255)
                                                                                                     tCACGGGCCGGCAAGATCAGTGTCAAGCCTGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  6:360931/136‑1 (MQ=255)
                                                                                                     tCACGGGCCGGCAAGATCAGTGGCACGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  3:22662/136‑1 (MQ=255)
                                                                                                     tCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGCCCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCCGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             >  4:161558/1‑136 (MQ=255)
                                                                                                     tCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  4:290170/136‑1 (MQ=255)
                                                                                                     tCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  2:295224/136‑1 (MQ=255)
                                                                                                     tCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  2:106442/136‑1 (MQ=255)
                                                                                                     tCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  6:97189/136‑1 (MQ=255)
                                                                                                     tCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  8:8277/136‑1 (MQ=255)
                                                                                                     tCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  8:4497/136‑1 (MQ=255)
                                                                                                     tCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  5:166362/136‑1 (MQ=255)
                                                                                                     tCACGGGCCGGCAAGATCAGTGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  1:33815/136‑1 (MQ=255)
                                                                                                     tCACGGGCCGGCAAGATCAGGGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  7:73417/136‑1 (MQ=255)
                                                                                                     tCACGGGCCGGCAAGAGCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  6:164421/136‑1 (MQ=255)
                                                                                                     tCACGGGCCGGAAAGATCTGTGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  3:71772/136‑1 (MQ=255)
                                                                                                     tCACGGGCCGGAAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  8:64996/136‑1 (MQ=255)
                                                                                                     tCACGGGCCGCCAAGATCAGTGGCAAGCCGGCAAA‑GCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  8:26138/136‑1 (MQ=255)
                                                                                                     tCACGGGACGGCATGATCTGTGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  7:120219/136‑1 (MQ=255)
                                                                                                     gcaggggCCGCCAAGAGCAGTGGCAACCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  4:283468/132‑1 (MQ=255)
                                                                                                     cccggggCCGCCAATATCATTGTCAAGCCTGAAAG‑GCGGCCCGCCTCGACATACTTGTCGCCGACCGTGGGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  3:291519/132‑1 (MQ=255)
                                                                                                     ccccGGGCCGGCAATATCTGTGCCAACCCGGAAAC‑GCCGCCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  3:99622/133‑1 (MQ=255)
                                                                                                     acaggggCCGCCAATATCTGGGGCAAGCCGGAAAA‑GCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  6:293904/132‑1 (MQ=255)
                                                                                                     acACGGGCCGTCAAGATATGTGGCAAGCCGCAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCTAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  7:107135/135‑1 (MQ=255)
                                                                                                      ccggggCCGGAAATTTCTTTGCCAACCCTGAAAG‑GCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  3:193535/132‑1 (MQ=255)
                                                                                                      ccggggCCGCCATTATCTGGGCCACGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  1:363865/132‑1 (MQ=255)
                                                                                                      cACGGTCGGTCAAGATCTGGGGCTAGCCGGCAAT‑GGCGGCCGCCTCGTCATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                             <  1:270511/135‑1 (MQ=255)
                                                                                                       aCGGGCCGGAAATATCAGGGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTc                           <  3:309409/136‑1 (MQ=255)
                                                                                                             cGGCATCATCTGTGCCACGCGGGAAAC‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGc                     <  2:178955/136‑1 (MQ=255)
                                                                                                             cGGCAAGATCAGTGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGc                     <  5:140516/136‑1 (MQ=255)
                                                                                                               gaaaGATCAGTGCCAAGCCGGAAAA‑GCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCtt                   <  5:239343/134‑1 (MQ=255)
                                                                                                               gCAAGATCAGGGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCtt                   <  5:187753/136‑1 (MQ=255)
                                                                                                                aaaGATCTGGGCCACGCCGGAAAC‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTa                  <  3:191169/135‑1 (MQ=255)
                                                                                                                 aaGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTATTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTa                  >  4:283975/1‑135 (MQ=255)
                                                                                                                      ctggggCACGCGGGAAAC‑GCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCgt            <  4:353964/132‑1 (MQ=255)
                                                                                                                      cAGTGGCAAGCCGGCAAA‑GCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCgt            <  8:89307/136‑1 (MQ=255)
                                                                                                                       aGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCgt            <  6:358534/135‑1 (MQ=255)
                                                                                                                         tGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCgtgt          <  4:340542/135‑1 (MQ=255)
                                                                                                                           gCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCa       <  5:298261/136‑1 (MQ=255)
                                                                                                                           gCAAGCCGGAAAC‑GCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCa       <  7:162092/136‑1 (MQ=255)
                                                                                                                             acgCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAgg     <  5:305718/134‑1 (MQ=255)
                                                                                                                             aaGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAgg     <  4:188657/136‑1 (MQ=255)
                                                                                                                             aaGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAgg     <  1:236073/136‑1 (MQ=255)
                                                                                                                                 cGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTAc  >  8:13009/1‑135 (MQ=255)
                                                                                                                                    |                                                                                                                                    
TGGGGGCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTAC  >  ACB143/6043064‑6043327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑