| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,719,426 | T→C | 42.3% | intergenic (‑321/+2) | kgtP ← / ← rrfG | alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,719,426 | 0 | T | C | 42.3% | 7.0 / 25.1 | 26 | intergenic (‑321/+2) | kgtP/rrfG | alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon |
| Reads supporting (aligned to +/- strand): ref base T (8/7); new base C (4/7); total (12/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01 | |||||||||||
ACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTAT > CP009273/2719280‑2719560 | acGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATAAGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGa > 2:2349/1‑149 (MQ=255) tGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGa > 1:83080/1‑144 (MQ=255) cAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTccc > 2:245078/1‑149 (MQ=255) tgggAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCATCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGTCATCTGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTActct < 2:59802/148‑1 (MQ=255) ggAAGCCGTATCCGTTGCTGAATCTGGCCTATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTAc < 1:24855/144‑1 (MQ=255) acGTATCCGTTGCTGAATCTGGCAAATGTGGGCGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGCTGCCTGGCAGTTCCCTACTCTCg > 1:144067/2‑144 (MQ=255) tATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGTATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGga < 2:5899/149‑1 (MQ=255) gCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGTTGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCcac < 2:89577/149‑1 (MQ=255) tGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGTTGGTCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCacac < 2:91800/149‑1 (MQ=255) gAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTTAGGTTGTCCTTTCGCATTATTTGTTGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACt < 2:90204/149‑1 (MQ=37) gAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCAACAGGCAATATAAACAACACCAATACAAAAAGGCCAACCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACt > 2:122272/1‑149 (MQ=25) aaTCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTGCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTa > 2:147463/1‑149 (MQ=255) aTCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCcac < 1:223807/144‑1 (MQ=255) ggAGTATAAGACGCGCAGCTTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTTTCGCATGGGGAGACCCCACACTACCATCGGCGCTACgg < 2:166405/149‑1 (MQ=255) tataAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCATTGCAAAAAGGCCATCCGTCAGGATGGACTTTCGCATAATTTGATGCCTGGCAGTTGCCTACTCTCGCATCGGGAGACCGCACACTACTATCGGCGCTCCg > 1:6896/1‑144 (MQ=21) cgcgCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGtgta > 1:153101/1‑141 (MQ=255) gTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGAATAGAAAATCTTTACTCTCGCTTGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGa > 1:79989/1‑144 (MQ=25) tttttttCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATgggg < 1:2057/144‑1 (MQ=255) cgcCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCACATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGaccccc > 2:8699/1‑149 (MQ=255) cgcCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAAATTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGaccacc > 2:140462/1‑149 (MQ=255) gcCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcg > 2:186409/1‑149 (MQ=255) gCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAc < 2:134032/149‑1 (MQ=255) cataaaGTCCACCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCTGTTCCCTCCTCTCGCATGGGGAGACCCCACAGTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcgcg < 1:177101/141‑1 (MQ=17) gATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCCTGGGGAGACCCCACACTACCATCGGCGCTCCGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAAt < 1:217418/144‑1 (MQ=255) ccTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTTAGGTGGGACCATCGTGCTACGGCCGCCAGGCAAATTCTGTTTTAt < 2:61687/149‑1 (MQ=255) tCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTa < 1:109652/144‑1 (MQ=33) | ACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTAT > CP009273/2719280‑2719560 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |