Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,420,394 G→T intergenic (‑127/+94) hisJ ← / ← argT histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,420,3940GT97.1% 108.3 / ‑6.8 34intergenic (‑127/+94)hisJ/argThistidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (16/17);  minor base C (1/0);  total (17/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTCGTAG  >  CP009273/2420250‑2420539
                                                                                                                                                |                                                                                                                                                 
tAGCACCAGTTTTTTCATAATGTCCTCATCTGTCAGTCTTTTTATAGGGTATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTa                                                                                                                                               <  2:168798/149‑1 (MQ=255)
      cAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTCTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTGGACGTATTATTTTTCATTATAACTTCTTAAGTATg                                                                                                                                         >  2:553955/1‑149 (MQ=255)
        gTTTTTTCATAATGTCCTCAACTGATAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCa                                                                                                                                       <  2:71099/149‑1 (MQ=255)
                   aTGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGcac                                                                                                                            <  2:79021/149‑1 (MQ=255)
                        cTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACgc                                                                                                                       >  2:126356/1‑148 (MQ=255)
                           aaCTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGcacca                                                                                                                          >  1:601884/1‑143 (MQ=255)
                                  aGTCTTTTTATAGGGAATATTTACAGGTTTGTTACCCTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATACACCACGATAGt                                                                                                                   >  1:395812/1‑143 (MQ=255)
                                  aGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGAATTTCAATGCACTACGCTAGTgcagca                                                                                                             >  2:683888/1‑149 (MQ=255)
                                   gTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTg                                                                                                                  <  1:485540/143‑1 (MQ=255)
                                           ataGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCg                                                                                                    <  2:117372/149‑1 (MQ=255)
                                               ggAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCa                                                                                                >  2:142582/1‑149 (MQ=255)
                                                gattatTTTCAGTTTTGTTACACTTTTCGTGCCATAAAATTGAGCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTGAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGc                                                                                                     <  1:49217/140‑1 (MQ=255)
                                                gAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGc                                                                                                     <  1:49132/143‑1 (MQ=255)
                                                        aCAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCa                                                                                             <  1:465782/143‑1 (MQ=255)
                                                             tttGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCCACAAACCTGGCGTGCACCataaga                                                                                        >  1:65934/1‑139 (MQ=255)
                                                              ttGTTTCACTTATCGTGACATAAAATTCACCATCAATGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCAtgatga                                                                                       <  1:581213/143‑1 (MQ=255)
                                                              gtGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTc                                                                                 <  2:92361/148‑1 (MQ=255)
                                                                  tACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATc                                                                             <  2:283632/149‑1 (MQ=255)
                                                                     acTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATCCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCAGGCGAGCACCAAGAAGATCATCCCacaaag                                                                          >  2:228092/1‑144 (MQ=255)
                                                                      cTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCa                                                                               >  1:608983/1‑143 (MQ=255)
                                                                           cGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGc                                                                    <  2:546830/149‑1 (MQ=255)
                                                                                        tGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCac                                                             >  1:34193/1‑142 (MQ=255)
                                                                                                       gatatCCCGCCCCCTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCAc                                              <  1:159407/142‑1 (MQ=255)
                                                                                                        atatCCCGCCTCGTTAGTCGTATTATTTCTCATTAAAAATTCTCAAGTACGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGAc                                       <  2:97290/149‑1 (MQ=255)
                                                                                                          atCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGt                                           >  1:255362/1‑143 (MQ=255)
                                                                                                               gCCCCGATCGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACa                                      >  1:110142/1‑143 (MQ=255)
                                                                                                                 cccGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCCTCAGGTACAGCTTCCCAGCGACGTATCAGTCCACGTAGACAtt                                    >  1:437815/1‑143 (MQ=255)
                                                                                                                     ttAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGaaa                          <  2:338036/149‑1 (MQ=255)
                                                                                                                         aCGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACt                      >  2:349004/1‑149 (MQ=255)
                                                                                                                              ttattTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTc                 <  2:285346/149‑1 (MQ=255)
                                                                                                                                   tttCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCAt            >  2:701781/1‑149 (MQ=255)
                                                                                                                                          aaaaaTTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTCg     >  2:299572/1‑149 (MQ=255)
                                                                                                                                           aaaaTTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATAATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAAACATTAAAGTCGAACGACTTTTTCACCATCTTGTctt    >  2:210455/1‑147 (MQ=255)
                                                                                                                                             aaTTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACACACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTCGTAg  <  2:541049/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
TAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTCGTAG  >  CP009273/2420250‑2420539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: