Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,154,028 G→A G235D (GGC→GAC)  ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,154,0280GA97.9% 290.0 / NA 94G235D (GGC→GAC) ptsGfused glucose‑specific PTS enzymes: IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (45/47);  minor base C (2/0);  total (47/47)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTA  >  CP009273/1153884‑1154171
                                                                                                                                                  |                                                                                                                                               
ctTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTAGGGGTTTCCGCgacgac                                                                                                                                             >  2:378295/1‑149 (MQ=255)
ctTAACAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACgacgac                                                                                                                                             >  2:383723/1‑149 (MQ=255)
     cAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTAATCGAACGTGGACTGGTACCGGTTGGTCTGAACCAGATCTGGAACGTACCTTTCCAGTTGCAGAGTGGTGAATACACCAACGCAGCAGGCCAGGTTTTCCACGACGACACTcc                                                                                                                                        >  2:507209/1‑149 (MQ=255)
       gAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCcgc                                                                                                                                      >  2:141078/1‑149 (MQ=255)
         atccGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGt                                                                                                                                    <  1:1218917/147‑1 (MQ=255)
            cGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTtat                                                                                                                                 >  2:558880/1‑149 (MQ=255)
                 gTTGCGTTTGGCATTTCTGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGTAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGc                                                                                                                            <  2:694879/149‑1 (MQ=255)
                  ttGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCg                                                                                                                           <  2:1031330/149‑1 (MQ=255)
                  ttGCGGTGGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCg                                                                                                                           <  1:1204239/149‑1 (MQ=255)
                      gTTTGGCATTTACGGTTGCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAGCGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGGCAGGCTTTCCAGGACGACATTCCGCGTTATATGGCGGGTg                                                                                                                       >  1:995723/1‑149 (MQ=255)
                         tGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGAcc                                                                                                                    >  2:448114/1‑149 (MQ=255)
                                 aCGGTTTCACCGATCGTTGCCCGGTACCGTTTGGTCTGAACACACAAAGAAGGGTTGGATTCCTTATGCTGATTGGTGTTTTCCCCTACTCAGCATGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCg                                                                                                            <  2:670592/149‑1 (MQ=255)
                                  cGGTTTCATCGGACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCgg                                                                                                           >  2:1216796/1‑149 (MQ=255)
                                  cGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGGATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCgg                                                                                                           >  1:751917/1‑149 (MQ=255)
                                  cGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCgg                                                                                                           <  1:833989/149‑1 (MQ=255)
                                  cGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCgg                                                                                                           <  2:499892/149‑1 (MQ=255)
                                  cGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTCACAGAGGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCTGACTGCgg                                                                                                           <  1:1059014/149‑1 (MQ=255)
                                   ggTTTCATCGAACGTTGCCTGGTAACCATTGGACTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACAAACGCCGCAGGTCAGGTTTTCCAAGACGACATTCCGCGTTATATGGCGCGTGACCCGACTGCggg                                                                                                          >  2:1398985/1‑149 (MQ=255)
                                          tCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTg                                                                                                   >  1:1390682/1‑149 (MQ=255)
                                            gAACGTTGCCTGGTACCGTTTGGGCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTc                                                                                                 <  1:308202/149‑1 (MQ=255)
                                             accggtGCCTGGGACCGTTTGGTCCGCACCACATCTGGACCGTACCTTTCCCGATGCAGATTGGTGAATACACCACCGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCt                                                                                                <  2:500402/144‑1 (MQ=255)
                                             aaCGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCt                                                                                                >  1:186152/1‑149 (MQ=255)
                                              aCGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCTGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCtg                                                                                               <  2:107076/149‑1 (MQ=255)
                                               cGTCGCCCGGTACCGTGTGGTCTGCACCACATCTGGAACGTCCCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTCTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCtgg                                                                                              <  2:429885/149‑1 (MQ=255)
                                                 cagCCTGGGACCCTTTGATCTCCACCACACCAGGGAGGCAACTTTCAAGCAATACATTGCATAAGCCACCAACGCAGCCGGTCCGGCGTCCCACAACAACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCtggtg                                                                                            <  2:670685/147‑1 (MQ=255)
                                                     cTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCtt                                                                                        >  2:171842/1‑149 (MQ=255)
                                                     cTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCtt                                                                                        <  1:690547/149‑1 (MQ=255)
                                                      tGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTc                                                                                       >  1:168803/1‑149 (MQ=255)
                                                      tGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTc                                                                                       >  2:535495/1‑149 (MQ=255)
                                                      tGGTACCGTTTGGTCCGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTc                                                                                       <  1:596001/149‑1 (MQ=255)
                                                      tGGTACCGGTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTc                                                                                       <  1:389857/149‑1 (MQ=255)
                                                       ggTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTcc                                                                                      >  2:1213575/1‑149 (MQ=255)
                                                       ggTACCGTTTGGTCTGCACAACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGt                                                                                                  <  2:503334/137‑1 (MQ=255)
                                                       ggTACCGTTTGGTCTGCACAACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACGGt                                                                                                  >  1:503334/1‑137 (MQ=255)
                                                        gTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCt                                                                                     <  1:141078/149‑1 (MQ=255)
                                                         tACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTg                                                                                    <  1:246143/149‑1 (MQ=255)
                                                          aCCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGt                                                                                   <  2:1296045/149‑1 (MQ=255)
                                                            cGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGTGTTTCAATGCGGGTGACCCGACTGCGGGTGAACTGTCTGGTGGCTTCCTGTTc                                                                                 >  1:1416982/1‑149 (MQ=255)
                                                             gTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCa                                                                                >  2:1188789/1‑149 (MQ=255)
                                                              tttGGTCTGCACCACATCTGGAACGTACCTTTCCACCAGCAGATTGGTGAATACACCAACTCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCaa                                                                               >  2:1032702/1‑149 (MQ=255)
                                                                tGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCaaaa                                                                             <  2:869102/149‑1 (MQ=255)
                                                                   tCTGCACCACATCTGGAACGTACCTTTCGAGATGCAGATTGGTGAATACACCAACGCAGCAGGACAGGTTTTCCACGACGACATTCCGCGGTATATGGCGGGTGACCCGACGGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGt                                                                          >  1:947312/1‑149 (MQ=255)
                                                                   tCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAACATGt                                                                          >  2:1038496/1‑149 (MQ=255)
                                                                   tCTGCACCACATCTGGAACGGACCTTTCCAGATGCAGATTGGTGAATACACCAGCGCAGCAGGTCAGGTTTTCGACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCTGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGt                                                                          <  2:246872/149‑1 (MQ=255)
                                                                    accaatctgcaTCTGGAAAGGTACGTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGt                                                                                   <  1:1179214/131‑1 (MQ=38)
                                                                        accacATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGACACGCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGt                                                                     <  1:1057322/149‑1 (MQ=255)
                                                                        accacATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGt                                                                     >  1:8906/1‑149 (MQ=255)
                                                                         ccacATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGc                                                                                          >  2:646729/1‑127 (MQ=255)
                                                                         ccacATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGc                                                                                          <  1:646729/127‑1 (MQ=255)
                                                                           cacaTCTGGAACGTACCTTTCCAGATGCGACTTTCTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCt                                                                   >  1:714185/1‑149 (MQ=255)
                                                                            acaTCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCGCGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTg                                                                  >  1:1076158/1‑149 (MQ=255)
                                                                            acaTCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTg                                                                  >  1:1098810/1‑149 (MQ=255)
                                                                            acaTCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCGGTTCAAAATGTACGGTCTg                                                                  >  1:613518/1‑149 (MQ=255)
                                                                                ctggaaaggtacgttCCAGGTGTAGAGTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGGAAACTGTCTGGTGGCTTCCTGTTCAAAATGTAAGGTATGGCAg                                                              >  1:754235/14‑149 (MQ=255)
                                                                                 tGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAAGGTACGGTCTGCCAGc                                                             >  1:865735/1‑149 (MQ=255)
                                                                                    aaCGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGc                                                          <  2:1407716/149‑1 (MQ=255)
                                                                                     aCGTACCTTCCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAACTTGCCCGTGCGGCCAGCTGcc                                                         <  2:1255015/149‑1 (MQ=255)
                                                                                      cGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCg                                                        <  1:448114/149‑1 (MQ=255)
                                                                                       ttACCTTTCCAGATGCACATTGGGGAATACACCAACGCAGCAGGTCAGGTTTTCCCCGACGACATTCCGCGTTATATGGCGGGTGACCCGACAGCGCGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGc                                                       <  1:647055/148‑1 (MQ=255)
                                                                                         aCCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCaa                                                     >  2:609926/1‑149 (MQ=255)
                                                                                         aCCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCaa                                                     >  2:40041/1‑149 (MQ=255)
                                                                                         aCCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCATTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCaa                                                     >  2:702266/1‑149 (MQ=255)
                                                                                          ccTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTAAGGTTTTCCACGCCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGGCTGCCAGCTGCCGCaag                                                    >  1:609985/1‑148 (MQ=255)
                                                                                           cTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAAtt                                                   <  2:168803/149‑1 (MQ=255)
                                                                                            tttCCGGATGCGGATTGGTCAATACACCAGGGGAGCAGGTCAGGTTTTGCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTg                                                  <  2:944896/149‑1 (MQ=255)
                                                                                            tttCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCTTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTg                                                  <  1:558880/149‑1 (MQ=255)
                                                                                            tttCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTg                                                  >  1:1193437/1‑149 (MQ=255)
                                                                                               ccAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTa                                               >  2:1313467/1‑149 (MQ=255)
                                                                                                cAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTAt                                              <  1:1188789/149‑1 (MQ=255)
                                                                                                       aGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGGCTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAACTGTACGGCCTGCCGGCTGCGGAAATTGCCATCTggacc                                       >  2:65509/1‑146 (MQ=255)
                                                                                                       aGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCAc                                       <  1:794101/149‑1 (MQ=255)
                                                                                                        gATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCAct                                      <  1:535495/149‑1 (MQ=255)
                                                                                                          ttGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCActct                                    <  1:171842/149‑1 (MQ=255)
                                                                                                           tGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTg                                   <  1:1004100/149‑1 (MQ=255)
                                                                                                              tGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCa                                        >  1:311837/1‑141 (MQ=255)
                                                                                                              tGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGGTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCa                                        <  2:311837/141‑1 (MQ=255)
                                                                                                                aaTACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAGACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCGGCCAGCTACCGGAATTGCTATCTGGCACTCTGCTaaa                              >  1:8292/1‑149 (MQ=255)
                                                                                                                   acacCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCggg                                                                                                          >  1:442224/1‑70 (MQ=255)
                                                                                                                   acacCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCggg                                                                                                          <  2:442224/70‑1 (MQ=255)
                                                                                                                   acacCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCa                           <  2:1193437/149‑1 (MQ=255)
                                                                                                                   acacCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCCGGTGGCTGCCGGCTAGAATTGCACGGTCTGGCAGCTGCCACCGTTGCTATTTGGCCCCCTGCTGAAcac                           >  1:1276012/1‑147 (MQ=255)
                                                                                                                     acCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTa                                                                         <  1:396834/101‑1 (MQ=255)
                                                                                                                     acCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTa                                                                         >  2:396834/1‑101 (MQ=255)
                                                                                                                     acCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCGCTCTGCTAAACCAGa                         >  1:442093/1‑149 (MQ=255)
                                                                                                                      ccAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGaa                        >  1:952147/1‑149 (MQ=255)
                                                                                                                       cAAAGCAGCAGGTCAGGTTTTCCACGCCGTTCTTCCGCGTTAGAAGGAGGGTGACTGGCCGGAGGGACAAAAGCTTGGTGGCTTGATGTGATAAGTGCACGGTGTGGCAGTAGCCGCAATTGCTATCTGGCACTATCATAAAcccaaaa                       >  2:574927/1‑144 (MQ=255)
                                                                                                                             agcagGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACcgcg                 <  1:1213575/149‑1 (MQ=255)
                                                                                                                             agcagGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACcgcg                 <  2:186152/149‑1 (MQ=255)
                                                                                                                              gcagGTCAGGTTTTCCACGACGACATTCCGCGTTCTATGGCGGGTGTCCTGATCACGTGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGGTCGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACcgcgc                <  2:1390682/149‑1 (MQ=255)
                                                                                                                                  gggcaggtTTTCCACGACGACATTCCGCGTTAGATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAGAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGaa             <  1:383723/146‑1 (MQ=255)
                                                                                                                                   gtcaggtTTTCCTCGACGACATTCCGCGTTATGTGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAGCCGCGCGaaa            <  2:737345/149‑1 (MQ=255)
                                                                                                                                    tcaggtTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGGAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACGCTGCTAAACCAGAAAACCGCGCGAAAg           >  1:660954/1‑149 (MQ=255)
                                                                                                                                       ggtTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTgg        >  1:328020/1‑149 (MQ=255)
                                                                                                                                         ttttCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGc      >  2:795306/1‑149 (MQ=255)
                                                                                                                                          tttCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCg     >  2:616214/1‑149 (MQ=255)
                                                                                                                                             ccACGACGACCTTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGta  <  2:270048/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
CTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTA  >  CP009273/1153884‑1154171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: