Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 753,013 G→T 56.0% E551* (GAA→TAA)  sdhA → succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273753,0130GT56.0% 21.9 / 67.5 59E551* (GAA→TAA) sdhAsuccinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base G (14/12);  new base T (19/14);  total (33/26)
Fisher's exact test for biased strand distribution p-value = 7.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCAGCGAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTC  >  CP009273/752869‑753161
                                                                                                                                                |                                                                                                                                                    
tCCAGCGAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATCGCCGCTTCGACTTCCCGGATCGCGATGATGaaaa                                                                                                                                                  >  1:1000681/1‑149 (MQ=255)
      gAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCGACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCAGACCGTGATACTGAAAACTGGCt                                                                                                                                            >  1:26184/1‑149 (MQ=255)
         ttCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGAACGTGAGGATTAAAACTg                                                                                                                                               >  2:710739/1‑143 (MQ=255)
           cAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGGAGGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGc                                                                                                                                             >  2:1046561/1‑143 (MQ=255)
              cacCCAGCGCGTTGGGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCtgt                                                                                                                                          <  2:759566/143‑1 (MQ=255)
                cccAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGc                                                                                                                                        <  2:1010809/143‑1 (MQ=255)
                  cggcgcGTCGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCt                                                                                                                                <  1:335441/147‑1 (MQ=255)
                  cAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGcacagccagtttta                                                                                                                                <  1:165059/149‑14 (MQ=255)
                     cgcgTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACGCCCTGTa                                                                                                                             >  1:663532/1‑149 (MQ=255)
                            aGTGCCTGGAACTGGAGAACCTGATGGTAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCTACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCa                                                                                                                      <  1:739377/149‑1 (MQ=255)
                             gTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTAGAATATTCGTATCGTGATGCTGACAACTGGCTGTGCCACTCCCTGTATCTGCCag                                                                                                                     >  1:619605/1‑149 (MQ=255)
                                ccTGGAACTGGATAACCTGATGGAACCGGCGTATGCAACGGCTGTTGCTGCCAACTTCCGTACCGAAAGCCGTGGTGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGc                                                                                                                        <  2:390355/143‑1 (MQ=255)
                                       cTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCCCTGCCTGTATCTGCCAGAGTCGGAATc                                                                                                           >  1:939308/1‑149 (MQ=255)
                                          gATACCCAGATGGAAACGGCGTATGCAACCGCTGTTTCGGCCAACTTCCGTACCGAAAGCCGTGGCGCGCCTAGCCGCTTCGACTTCCCGGATGGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGa                                                                                                              <  2:406354/143‑1 (MQ=255)
                                            tAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGa                                                                                                      >  1:139918/1‑149 (MQ=255)
                                             aaCCTGACGGAAACGGCGGATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATc                                                                                                           <  2:535657/143‑1 (MQ=255)
                                               ccTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGCCGGAATCCATGAcac                                                                                                   >  1:1062990/1‑147 (MQ=255)
                                                cTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTGTCTGCCAGAGGCGGAATCCAt                                                                                                        >  2:771628/1‑143 (MQ=255)
                                                 tGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCCTAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACgcgc                                                                                                 >  1:260828/1‑149 (MQ=255)
                                                   aTGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGCTTAAAACTGGCTGGGCCACTCCCTGTATCTGCCAGAGTCGGAACCCATGACGCGCCg                                                                                               >  1:1110931/1‑149 (MQ=255)
                                                   aTGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCACATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGAGCCg                                                                                               >  1:1109867/1‑149 (MQ=255)
                                                   aTGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCACATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAAAGTCGGAATCCATGACGCGCCg                                                                                               >  1:990328/1‑149 (MQ=255)
                                                     ggtaaCGGCGTATGCAACGGCTGTTTCCGCCACCTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGAcgc                                                                                                   <  2:280381/140‑1 (MQ=255)
                                                     ggAAACGGCGTATGCAACGGCTGTATCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGCCGGAATCCAAGAcgc                                                                                                   >  2:281996/1‑143 (MQ=255)
                                                      gAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTCGCGCGCATAGCCGCTTCGACTTCCCGGATGGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGACAGAGTCGGAATCCATGAcgcg                                                                                                  >  2:188175/1‑143 (MQ=255)
                                                            gCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCaa                                                                                      <  1:1252229/149‑1 (MQ=255)
                                                            gCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCACGACGCGCCGAAg                                                                                            <  2:903489/143‑1 (MQ=255)
                                                             cGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAAc                                                                                     <  1:1031410/149‑1 (MQ=255)
                                                               tATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACAt                                                                                   <  1:1056951/149‑1 (MQ=255)
                                                               tATGCAACGGCTGTTTCCGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGt                                                                                         <  2:756735/143‑1 (MQ=255)
                                                               gaTGCAACGTCTGTTCCGGCCACCTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTTGACTTCCCGGATCGTGACTATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGt                                                                                         <  2:850726/142‑1 (MQ=255)
                                                                          tGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCACCATGGAAc                                                                              >  2:872953/1‑143 (MQ=255)
                                                                           gTTTCTGCCAACTTCCGTACCGACAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGGGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAAcc                                                                             <  2:962755/143‑1 (MQ=255)
                                                                           gTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAAcc                                                                             <  2:337513/143‑1 (MQ=255)
                                                                            tttCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCAGCTTCGACTTCACGGATCGTGATGATGAAAACTGGCTGTGCCACTGCCTGTATCTGCCAGAGTCGGAATCCATGAGGCGCCGAAGCGCACACATGGGACCGAAACTg                                                                      >  1:316845/1‑149 (MQ=255)
                                                                               cTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGCTCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCCACATGGAACCGAAGCTgcgc                                                                   >  1:386167/1‑149 (MQ=255)
                                                                               cTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCGGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGaaa                                                                         >  2:900259/1‑143 (MQ=255)
                                                                               cTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGaaa                                                                         <  2:766412/143‑1 (MQ=255)
                                                                                tGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGcc                                                                  >  1:513288/1‑149 (MQ=255)
                                                                                      attCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGcc                                                                  >  2:698196/2‑143 (MQ=255)
                                                                                           gTACCGAGAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCccg                                                       <  1:161577/149‑1 (MQ=255)
                                                                                           gTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCCCTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCccg                                                       >  1:1149890/1‑149 (MQ=255)
                                                                                            tACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCccgc                                                      >  1:557868/1‑149 (MQ=255)
                                                                                            tACCGAAAGCCGTGACGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGGCAACATGAAAACGAAACTGCGCCCGGCGTTCccgc                                                      >  1:353515/1‑149 (MQ=255)
                                                                                                     ccGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCCCTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGa                                                   >  2:456303/1‑143 (MQ=255)
                                                                                                      cGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCg                                            <  1:1185690/149‑1 (MQ=255)
                                                                                                        tGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATCACGCGCCCAAGCGTCAACATGGACCCGAAACTGCGCCCGGCCTTCCCGCCGAAGa                                                >  2:954687/1‑143 (MQ=255)
                                                                                                           cgcgcATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGtactt                                       >  1:1227440/1‑149 (MQ=255)
                                                                                                            gcgcATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGtactta                                      <  1:1297433/149‑1 (MQ=255)
                                                                                                                aTAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTaa                                  <  1:1098446/149‑1 (MQ=255)
                                                                                                                      gCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGga                            >  1:693272/1‑149 (MQ=255)
                                                                                                                      gCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATTTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACCTTGCACCGAAACTGCGCCCGGCAATCCCGCCGCAGACTCATACTTACTaa                                  >  2:618380/1‑143 (MQ=255)
                                                                                                                        ttCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTCATGCGgaga                          >  1:1215335/1‑149 (MQ=255)
                                                                                                                                cccGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACa                        <  2:21754/143‑1 (MQ=255)
                                                                                                                                 ccGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAAt                 >  1:71913/1‑149 (MQ=255)
                                                                                                                                     aTCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATgaga             <  1:995781/149‑1 (MQ=255)
                                                                                                                                        gTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATg                <  2:441124/143‑1 (MQ=255)
                                                                                                                                        gTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTc          <  1:662299/149‑1 (MQ=255)
                                                                                                                                        gTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAGCATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTAGTAATGCGGAGACAGGAAAATg                >  2:814752/1‑143 (MQ=255)
                                                                                                                                                gaAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTc  >  1:1289438/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
TCCAGCGAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTC  >  CP009273/752869‑753161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑