Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,860,628 T→A 38.8% I95F (ATC→TTC)  rpoS ← RNA polymerase, sigma S (sigma 38) factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,860,6280TA38.8% 95.4 / 121.0 121I95F (ATC→TTC) rpoSRNA polymerase, sigma S (sigma 38) factor
Reads supporting (aligned to +/- strand):  ref base T (39/34);  new base A (26/20);  total (66/55)
Fisher's exact test for biased strand distribution p-value = 8.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

TTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAA  >  CP009273/2860481‑2860775
                                                                                                                                                   |                                                                                                                                                   
ttGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGatc                                                                                                                                                    <  1:1058631/149‑1 (MQ=255)
    gaAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATcc                                                                                                                                                >  1:176658/1‑149 (MQ=255)
    gaAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATcc                                                                                                                                                <  1:677394/149‑1 (MQ=255)
       gCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCcggc                                                                                                                                             <  1:769806/149‑1 (MQ=255)
        cGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCCAGTTACGCTCGACCATCcggca                                                                                                                                            >  1:457668/1‑148 (MQ=255)
        cGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCcggcg                                                                                                                                            <  1:198266/149‑1 (MQ=255)
         ggACACCACGTTCCGGGTCAAACTACTCGACCGCGCGGATCAGCCCCCGGTTGGCCTCTCCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGaacatc                                                                                                                                                 <  2:360910/143‑1 (MQ=255)
         ggAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCcggcgg                                                                                                                                           <  1:1291441/149‑1 (MQ=255)
          gAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCggcggc                                                                                                                                          >  1:540281/1‑149 (MQ=255)
               cACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCGTCcggcgg                                                                                                                                           >  2:1126208/1‑143 (MQ=255)
                aCGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAgg                                                                                                                                    >  1:1219368/1‑149 (MQ=255)
                aCGTCCCGGGTCAAACTGCCCTCCCGCGCGGATCAGCCCCAGGTGGCCCTCTGCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAgg                                                                                                                                    <  1:1115105/149‑1 (MQ=255)
                 cGTCCCGGGTCAAACTTCTCTACCGCGTTTATCGGCCCCAGAGAGCCCTCTTGGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGc                                                                                                                                   <  1:298529/149‑1 (MQ=255)
                       gggTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACAtc                                                                                                                             >  1:826199/1‑149 (MQ=255)
                         gTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCCAACATCCGGCGGCGAGAGGGGa                                                                                                                                 >  2:605373/1‑143 (MQ=255)
                           cAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCTGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGAcc                                                                                                                               >  2:600834/1‑142 (MQ=255)
                           cAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACa                                                                                                                               <  2:764591/143‑1 (MQ=255)
                            aaaCTTCTCTACCGCGCGGATCAGCCCCAGGGTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCCATTTTTACCACCCCACGCAAGGTACGCTCGATCCTCcggcgcccgaggccgccac                                                                                                                              >  2:437787/1‑129 (MQ=255)
                             aaCTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACAACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGCTAATCTCCATCATCCGGCGGCgagcgcccacacc                                                                                                                             >  2:214132/1‑133 (MQ=255)
                                ttCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTcc                                                                                                                          >  2:562099/1‑143 (MQ=255)
                                ttCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCGTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAg                                                                                                                    <  1:563433/149‑1 (MQ=255)
                                 tctcTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCCACATCGCCa                                                                                                                         <  2:865044/143‑1 (MQ=255)
                                  ctctACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCAc                                                                                                                        >  2:1048886/1‑143 (MQ=255)
                                    ctACCGCGCGGATCAGCCCCAGGTTGCCCCCTACGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTgcg                                                                                                                <  1:425513/149‑1 (MQ=255)
                                       ccGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGa                                                                                                             >  1:567976/1‑149 (MQ=255)
                                       ccGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGCCGGCACCTccccccact                                                                                                                   >  2:1037659/1‑136 (MQ=255)
                                       ccGCGCGGATCAGCCCCAGGGTGCCCTCTTCGATAAGGTCCAGCAACGCCAGCCCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGGGAGGCGACATCTCCACGCAGt                                                                                                                   >  2:701194/1‑143 (MQ=255)
                                         gcgcgGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTgc                                                                                                                 >  2:955162/1‑143 (MQ=255)
                                          cgcgGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGAcgc                                                                                                          <  1:450911/149‑1 (MQ=255)
                                          cgcgGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGAcgc                                                                                                          >  1:22950/1‑149 (MQ=255)
                                                tCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCCGTGCGCGACGCGCaaaa                                                                                                    >  1:416747/1‑149 (MQ=255)
                                                   ttcccAGGTTGCCCTCTTCGATCAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCa                                                                                                       <  2:1134463/141‑1 (MQ=255)
                                                   gCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCa                                                                                                       <  2:1077899/143‑1 (MQ=255)
                                                   gCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATaa                                                                                                 >  1:177880/1‑149 (MQ=255)
                                                    cgcccggTTTCCCTCTTCGATAAGGTCCAGCAACGCCAGCCCACGATTGGCATAACGGCGGGTAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATaaa                                                                                                <  1:615509/144‑1 (MQ=255)
                                                    ccccAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATaaa                                                                                                <  1:121178/149‑1 (MQ=255)
                                                    ccccAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGGGGCGACATCTCCACGCAGTGCGCGACGCGCAAAGTaaa                                                                                                >  1:359689/1‑149 (MQ=255)
                                                    ccccAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATaaa                                                                                                >  1:836934/1‑149 (MQ=255)
                                                        aGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGGGGCGACATCTCCACGCAGTGCGCGGCGCGCAAGATAAActtg                                                                                            >  1:344606/1‑148 (MQ=255)
                                                        aGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAAAGGCGACATCTCCACGCCGTGCGCGACGCCCAAAATAAActtc                                                                                            >  1:491913/1‑149 (MQ=255)
                                                          gTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTCCTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAActtctt                                                                                          <  1:787339/149‑1 (MQ=255)
                                                                ctcgTCGATAAGGTACAGCAACGCCAGACCACGTTTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAActtctt                                                                                          <  2:306394/143‑1 (MQ=255)
                                                                 tctcCGATAAGGTCCAGCAACGCCAGGCCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGc                                                                                   <  1:971283/149‑1 (MQ=255)
                                                                 tctTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCCTCCGGCGGCGAGAGGCGCCATCTCCACGCAGTGCGCGACGCGCAAAATAAActtattc                                                                                         >  2:387938/1‑143 (MQ=255)
                                                                 tctTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGc                                                                                   <  1:1264216/149‑1 (MQ=255)
                                                                       aTAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTaa                                                                             <  1:1032308/149‑1 (MQ=255)
                                                                        tAAGGTCCAGCAACGCCAGCCCACGAGTGCCATATCGGGGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCTAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGcc                                                                                  <  2:132551/143‑1 (MQ=255)
                                                                        caaGGCCCAGCAGCGCCAGACCACGTTTGCCAGAGCGGTGGGCAATTTTTACCACCGGGCGCAAGTTACTCTCGATCCTGCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGcc                                                                                  <  2:621569/142‑1 (MQ=255)
                                                                         aaGGTCCAGAATCGACTGCCAAAGAATGCCATAACGGCGGGCACGTTTTCAGTATAGCCGAAAGTTACTCTCGAACATCTGGCGCCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACa                                                                           <  1:1241850/149‑1 (MQ=255)
                                                                            gTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCCCGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGCCCGTTa                                                                              >  2:636230/1‑143 (MQ=255)
                                                                            gTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAgtg                                                                        >  1:256980/1‑149 (MQ=255)
                                                                            gTCCAGCAACGCCAGACCACGATTGCAATGAGGTCGTGAAATTTTTACCACCATACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGtca                                                                              >  2:246435/1‑141 (MQ=255)
                                                                             tCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTCCCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAgtgg                                                                       <  1:481432/149‑1 (MQ=255)
                                                                              ccAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAgtggt                                                                      <  1:1247205/149‑1 (MQ=255)
                                                                                aGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAg                                                                          >  2:731473/1‑143 (MQ=255)
                                                                                  cAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAt                                                                  >  1:357572/1‑149 (MQ=255)
                                                                                   aaCGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATa                                                                 <  1:450148/149‑1 (MQ=255)
                                                                                    aCGCCAGGCCACGATTGCCAGAGCGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACACAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATaa                                                                <  1:1271927/149‑1 (MQ=255)
                                                                                    aCGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATaa                                                                <  1:95077/149‑1 (MQ=255)
                                                                                      gCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTAAATAAcc                                                              >  1:752341/1‑149 (MQ=255)
                                                                                       ccAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGTCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCa                                                             >  1:786483/1‑149 (MQ=255)
                                                                                          gcccACGATTGCCATAACGGCGGGCAATTTTTCCCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAAtc                                                          <  1:641568/147‑1 (MQ=255)
                                                                                             cACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCa                                                       >  1:305620/1‑149 (MQ=255)
                                                                                             cACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCa                                                       <  1:118345/149‑1 (MQ=255)
                                                                                             cACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCa                                                       <  1:709670/149‑1 (MQ=255)
                                                                                             cACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCa                                                             <  2:886974/143‑1 (MQ=255)
                                                                                             cACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGCTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTCACAGTGGTGAATAACCa                                                             >  2:467198/1‑143 (MQ=255)
                                                                                              aCGATTGCCAGAACGGCGGGCAGTTTTTACCACCAGACGCAAGTTGCTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCaa                                                            <  2:903198/143‑1 (MQ=255)
                                                                                               cgttcgCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCAcc                                                     <  1:1204095/144‑1 (MQ=255)
                                                                                                  ttGCCATAACGGCGGGCAATTTTTACCCCCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGATAACTGTGGTGAATAACCAATCTCACCAAg                                                  <  1:674789/149‑1 (MQ=255)
                                                                                                    gCCACAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCAc                                                      <  2:663599/143‑1 (MQ=255)
                                                                                                     ccATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAAGTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCAcc                                                     >  2:685507/1‑143 (MQ=255)
                                                                                                     ccATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAACCTCACCAAggca                                               >  1:506235/1‑147 (MQ=255)
                                                                                                       aTAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTaaa                                             <  1:450544/149‑1 (MQ=255)
                                                                                                        tAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCGCACCaaa                                                  >  2:932368/1‑142 (MQ=255)
                                                                                                                ggCAATTTTTACCACCAGACGCAAGTTACTCTCGACCTACCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATATCCAAGCTCACCAAGGTAAAGCTGAGTcg                                    <  1:850601/149‑1 (MQ=255)
                                                                                                                 gCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGACTAACCAATCTCACCAAGGTAAAGCTg                                         >  2:695410/1‑143 (MQ=255)
                                                                                                                   aaTTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGt                                 >  1:544797/1‑149 (MQ=255)
                                                                                                                    aTTTTTCCCACCTGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTc                                <  1:711106/149‑1 (MQ=255)
                                                                                                                     tttttACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGAGAAAATTGGTTATTAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTcc                               >  1:426436/1‑149 (MQ=255)
                                                                                                                       tttACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTcgc                                   <  2:487218/143‑1 (MQ=255)
                                                                                                                          accaccAGACGCAAGTTACTCTCGAACATCCGGCGTCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTc                                <  2:1136550/143‑1 (MQ=255)
                                                                                                                             accaGACGCAAGCTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCTAAATAACCTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCaa                             <  2:403503/143‑1 (MQ=255)
                                                                                                                              ccaGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGc                      >  1:1042523/1‑149 (MQ=255)
                                                                                                                                aGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCGAAGTAAGCTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGATGAAGCTGAGTCTCGTCCAACACACGCtg                    >  1:757060/1‑149 (MQ=255)
                                                                                                                                  aCGCAAGTTACTCGCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGGAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTGACCAAGGTAAAGCTGAGTCGCGTCCCacacac                        >  2:583322/1‑143 (MQ=255)
                                                                                                                                    gCAAGTTACTCTCGAAAATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGGTAACAGTGGTGAACAGCCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCtgtgtg                <  1:1017300/149‑1 (MQ=255)
                                                                                                                                     cAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTgg               >  1:129772/1‑149 (MQ=255)
                                                                                                                                       aGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGGCCAACACACGCTGTGTGGCt             >  1:290565/1‑149 (MQ=255)
                                                                                                                                        gTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAAGAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCCCGTCCACCCCACGcgcgg                  >  2:961146/1‑139 (MQ=255)
                                                                                                                                           aCTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTgg               <  2:154408/143‑1 (MQ=255)
                                                                                                                                           aCTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTGCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTgg               <  2:199259/143‑1 (MQ=255)
                                                                                                                                             tctcGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACGTCTGCGTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCt             >  2:1138349/1‑143 (MQ=255)
                                                                                                                                             tctcGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTTACGGTTTGTAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGc       <  1:571459/149‑1 (MQ=255)
                                                                                                                                             tctcGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCt             >  2:354741/1‑143 (MQ=255)
                                                                                                                                              ctcGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCg      <  1:1188256/149‑1 (MQ=255)
                                                                                                                                                cGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAACAACCAATCTCCCCAAGGTAAAGCTGAGTCGCGTCCAACACACACTGTGTGGCcccc          >  2:437903/1‑143 (MQ=255)
                                                                                                                                                 gATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCt         >  2:1150262/1‑143 (MQ=255)
                                                                                                                                                  atcatcCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATaa  >  1:389445/1‑149 (MQ=255)
                                                                                                                                                  atcaccCGGCGGCGAAAGGCGACATCTCAACGCAGAGCTCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTg        <  2:675885/143‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAA  >  CP009273/2860481‑2860775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑