Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	416,398	G→T	7.7%	intergenic (+32/‑44)	brnQ → / → proY	branched‑chain amino acid transport system 2 carrier protein; LIV‑II transport system for Ile, Leu, and Val/proline‑specific permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	416,398	0	G	T	7.7%	218.6 / 6.2	78	intergenic (+32/‑44)	brnQ/proY	branched‑chain amino acid transport system 2 carrier protein; LIV‑II transport system for Ile, Leu, and Val/proline‑specific permease
Reads supporting (aligned to +/- strand):  ref base G (37/35);  new base T (2/4);  total (39/39)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01

CGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGA  >  CP009273/416250‑416545                                                                                                                                                     |                                                                                                                                                    cGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCCCTGATCATTTGTTTTAACCACGGGGCTg                                                                                                                                                     <  1:720471/149‑1 (MQ=255) cGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTg                                                                                                                                                     >  1:378486/1‑149 (MQ=255)          cTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGcccc                                                                                                                                            >  1:891749/1‑149 (MQ=255)          cTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGcccc                                                                                                                                            <  1:477254/149‑1 (MQ=255)           tgcccGAACAGGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCg                                                                                                                                           <  1:34958/146‑1 (MQ=255)              ccGAACAAGGTCTGGCGTGGTTAGTGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTgg                                                                                                                                        <  1:905065/149‑1 (MQ=255)                 aaCAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGATTCTGGCCATTATCTGGGATCGCACGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCGCGTGGttt                                                                                                                                     >  1:1115390/1‑149 (MQ=255)                   cAAGGGCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTg                                                                                                                                         <  2:388427/143‑1 (MQ=255)                    aaGGTCTGGCGTTGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTATCCACGGGGCTGCGATGCCCCGTgg                                                                                                                                        <  2:897410/143‑1 (MQ=255)                      ggTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTAt                                                                                                                                >  1:52218/1‑149 (MQ=255)                       ctCTTGCGTGGTTAATGCCAACAGTGGTGATGGTGCTTCTGGCCATTATCTGGGATCGCGCGGCCGGTCGTCAGGTGCCCTCCAGCGCGCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTAtt                                                                                                                               <  1:22312/148‑1 (MQ=255)                         cTGGCGGGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGGCCCGTGGTTTTTTATtgt                                                                                                                             <  1:957592/149‑1 (MQ=255)                             cgcggTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGGCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTAtt                                                                                                                               <  2:243450/140‑1 (MQ=255)                             cGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTAtt                                                                                                                               <  2:326930/143‑1 (MQ=255)                             cGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTg                                                                                                                         >  1:414603/1‑149 (MQ=255)                               ttgTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCATCGCAGCCCCGTGGttaaaacaaat                                                                                                                             <  2:1116451/141‑10 (MQ=38)                                 gTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATgg                                                                                                                     <  1:420209/149‑1 (MQ=255)                                  ttAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATggg                                                                                                                    >  1:1261824/1‑149 (MQ=255)                                    aaTGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTGTTTTATTGTGTTGa                                                                                                                        >  2:914991/1‑143 (MQ=255)                                    aaTGCCAACAGTGGGGATGGTGGTTCTGGCCATTAGCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATGGTGTTGa                                                                                                                        >  2:1140019/1‑143 (MQ=255)                                        ccactcgaggagATGGGGGTTCTGTCCATTATCTGGGAGCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGCTTTAACCACGAGGCTGCGATGTCCCGGGGTTTTTTATTGTGTTGATGGGTTAGGa                                                                                                              <  1:120487/138‑1 (MQ=255)                                         cAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGt                                                                                                                   >  2:969139/1‑143 (MQ=255)                                          aaCAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGATCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTACTGTGTTGATGGGtt                                                                                                                  >  2:839879/1‑143 (MQ=255)                                             aGTGGTGATGGTGGTTCTGGCCATTATCTGGGATGGCGCGGCAGGTGGTGAGGTGACCTCCAGCGCTCACTAAATCACTGAACGTTTGTTTTAACCACGGGGGTGGGGTGCCCCGTGGTTGTTTGTTGTGTTGTTGGGTTAgg                                                                                                               >  2:339691/1‑143 (MQ=255)                                               tggtgATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCGCTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTGTTTATTGTGTTGATGGGTTAGGAATTGATg                                                                                                       >  1:361589/1‑149 (MQ=255)                                               tggtgATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGCGGTTTTTTATTGTGTTGATGGGTCAGGaa                                                                                                             >  2:1064183/1‑143 (MQ=255)                                                ggtgATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGCACCTTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGaag                                                                                                            >  2:306371/1‑142 (MQ=255)                                                   gATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATGTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTTATGTGTTGATGGGTTAGGAATTGATGGaaa                                                                                                   <  1:797324/149‑1 (MQ=255)                                                   gATGGTGGTTCGGGCCAGTGTCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGATGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGaaa                                                                                                   <  1:420359/149‑1 (MQ=255)                                                     tggtggTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATg                                                                                                       <  2:309252/143‑1 (MQ=255)                                                       gtggtTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTaa                                                                                               >  1:770719/1‑149 (MQ=255)                                                         ggtTCTGGCCATTATCTGGGAACGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCACACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTAATTGTGTTGCTGGGTTAGGAATTGATGGaaa                                                                                                   >  2:388068/1‑143 (MQ=255)                                                             cTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTaa                                                                                               >  2:585678/1‑143 (MQ=255)                                                                 acATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGGGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTa                                                                                     <  1:352835/148‑1 (MQ=255)                                                                  cATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTaa                                                                                    >  1:390503/1‑149 (MQ=255)                                                                    cgggtacttagcccacgctttagcttgttcttactttccatcaattcctaacccatcaacacaataaaaAACCACGGGGCATCGCAGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAg                                                                                  <  1:488839‑M1/80‑1 (MQ=255)                                                                         tGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTaaa                                                                                   <  2:1048790/143‑1 (MQ=255)                                                                          gggATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTggg                                                                            >  1:197232/1‑149 (MQ=255)                                                                            gATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCg                                                                                >  2:640828/1‑143 (MQ=255)                                                                            gATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCg                                                                                >  2:561429/1‑143 (MQ=255)                                                                                 cgcgGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTa                                                                     >  1:1088854/1‑149 (MQ=255)                                                                                     gCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAg                                                                       <  2:527164/143‑1 (MQ=255)                                                                                        ggTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGAGGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCca                                                              <  1:253681/149‑1 (MQ=255)                                                                                         gtcgtcAGGTGACCTCCAGCGCTCACTAAATCAGTGAACATTTGTTTTACCCACGGGGCTGCGATGCCCCGCGGTTTTTTATTGGGTTGATGGGTTAGGAATTGCTGGAACGTCAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCcac                                                             >  1:836886/1‑149 (MQ=255)                                                                                             tcAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATAc                                                         >  1:574429/1‑149 (MQ=255)                                                                                                 gTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCtt                                                     >  1:66915/1‑149 (MQ=255)                                                                                                  tGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCttt                                                    <  1:811418/149‑1 (MQ=255)                                                                                                   tactttccatcaattcctaacccatcaacacaataaaaAACCACGGGGCATCGCAGCCCCGAGGATTTTTATTGTGTTGATGGGTTAGGAATTCATGGAAAGTAAGAACAAGCTAAAGCTGGGGCTAAGTACCCGCCACATAc                                                         <  2:98786‑M1/105‑1 (MQ=255)                                                                                                   gACCTCCAGCGCTCACTAAATCCCTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTa                                                   <  1:64836/149‑1 (MQ=255)                                                                                                     ccTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAGCAGCGGGGCTGCGAGGCCCCGTGGATTTTTATGGTCTTGATGGGTTAGGGATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCGCCCACATCCGc                                                       >  2:2618/1‑143 (MQ=255)                                                                                                     ccTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTAGTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGc                                                       <  2:215880/143‑1 (MQ=255)                                                                                                        gcAGGGCTCACTAAGGCGGGGAACATTTGTGTGAGCGACGGGGCGGCGGTGGCGCGTGGTTTTTTATTGTGTTGATGGGGTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCa                                              <  1:1179462/148‑1 (MQ=255)                                                                                                         cAGCGCTTACTACATCACCGACCATTTGTTTTAACCACGTGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTa                                                   <  2:587618/143‑1 (MQ=255)                                                                                                         cAGCGCTCACTAAATCCCTGAACATTTGTTTTAACCACGGGGTTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTTCCCGCCACATACGCTTTa                                                   <  2:282063/143‑1 (MQ=255)                                                                                                              cTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGt                                        >  1:1087862/1‑149 (MQ=255)                                                                                                              cTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGAGGCCCCGTGGTTTATTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTCCCCGCCACATACGCTTTATGGCa                                              <  2:776079/143‑1 (MQ=255)                                                                                                                   tAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGc                                   >  1:177381/1‑149 (MQ=255)                                                                                                                   tAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGAGTTCAGc                                   >  1:230780/1‑149 (MQ=255)                                                                                                                     aacccatcaacacaataaaaAACCACGGGGCATCGCAGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGtt                                       >  2:420142‑M1/21‑143 (MQ=255)                                                                                                                      aTCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCaag                                >  1:1119504/1‑148 (MQ=255)                                                                                                                       tCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGTGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAAtt                               <  1:894265/149‑1 (MQ=255)                                                                                                                       tCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAAtt                               <  1:993770/149‑1 (MQ=255)                                                                                                                       tCACGGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAAtt                               <  1:985555/149‑1 (MQ=255)                                                                                                                        cACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAg                                    <  2:608252/143‑1 (MQ=255)                                                                                                                               cATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTgg                             <  2:946293/143‑1 (MQ=255)                                                                                                                               cATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTgg                             <  2:438024/143‑1 (MQ=255)                                                                                                                                   tGTTTTAACCACTGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGTTGGGTTAGGAATTGGTGGAAAGTAAGTACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCAcc                         <  2:404134/143‑1 (MQ=255)                                                                                                                                     ttttAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGtt                 >  1:53497/1‑149 (MQ=255)                                                                                                                                     ttttAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGAAATTGGCACCGGGcgggt                 >  1:547452/1‑145 (MQ=255)                                                                                                                                       ttAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACGCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGc                     >  2:126608/1‑143 (MQ=255)                                                                                                                                       aaAACCACGGGGCATCGCAGCACCGTGGTTTTTTATTGTGTTGATGGGATAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCtttttt               <  1:197460‑M1/147‑1 (MQ=255)                                                                                                                                        tacCCACGGGGTTGCGATGCCCCGTGGTTTTTTATTGTGTTTATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTa              >  1:509285‑M1/4‑149 (MQ=255)                                                                                                                                        gAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAATTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTa              >  1:1154845‑M1/2‑149 (MQ=255)                                                                                                                                        aAACCACGGGGCATCGCAGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTa              >  1:114519‑M1/2‑149 (MQ=255)                                                                                                                                         AACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCGAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTg                   >  2:723786‑M1/1‑143 (MQ=255)                                                                                                                                            CACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGAGGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGttt                >  2:1116692‑M1/1‑143 (MQ=255)                                                                                                                                               GGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAAGTGGCCCCGGGCTGTTTTACGGTTCg       <  1:8305‑M1/149‑1 (MQ=255)                                                                                                                                                GGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGTAATGGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCCAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACg            >  2:555579‑M1/1‑143 (MQ=255)                                                                                                                                                    tGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGa  <  1:1071584/149‑1 (MQ=255)                                                                                                                                                    tGCGATGCCCCGTGGTTTTTTATTGCGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTc        <  2:337216/143‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    CGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGA  >  CP009273/416250‑416545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑