Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,964,513 G→A 6.2% intergenic (+21/‑117) ygdQ → / → ygdR UPF0053 family inner membrane protein/DUF903 family verified lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,964,5130GA 6.2% 223.7 / 3.1 78intergenic (+21/‑117)ygdQ/ygdRUPF0053 family inner membrane protein/DUF903 family verified lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (34/39);  new base A (2/3);  total (36/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

TTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCT  >  CP009273/2964371‑2964656
                                                                                                                                              |                                                                                                                                               
ttctcgtgcttCGGGATAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAACTCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCg                                                                                                                                                 <  2:644899/134‑1 (MQ=255)
 tACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGc                                                                                                                                          >  1:1023209/1‑149 (MQ=255)
 tACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGc                                                                                                                                          >  1:935861/1‑149 (MQ=255)
    ccTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTa                                                                                                                                       >  1:801482/1‑149 (MQ=255)
    ccTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCTGCGGGAGGGCGTa                                                                                                                                       >  1:216013/1‑149 (MQ=255)
    ccTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTa                                                                                                                                       <  1:863848/149‑1 (MQ=255)
     cTGCTTCTGGAAGGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTGCTTCTCTATTGCTGGTGAAAGCCTCAACGTGATTCGCAGCAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCgggggg                                                                                                                                            >  2:391649/1‑143 (MQ=255)
     cTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTCATTCGCGGTGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAAGCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAgg                                                                                                                                            >  2:561016/1‑143 (MQ=255)
      ctaTTCTGGAGAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTCCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTact                                                                                                                                     <  1:472067/147‑1 (MQ=255)
        aTTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATTTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCg                                                                                                                                         <  2:149899/143‑1 (MQ=255)
        aTTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCg                                                                                                                                         <  2:385781/143‑1 (MQ=255)
        aTTCTGGAAAGTTCCGACATCCACGTACCGAAAGGTTACATCTACTGCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactca                                                                                                                                   <  1:1169135/149‑1 (MQ=255)
         ttCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGCGGGCGt                                                                                                                                        >  2:182608/1‑143 (MQ=255)
         ttCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactcac                                                                                                                                  >  1:557103/1‑149 (MQ=255)
            cggAAAGTCTCGACATCCACCGACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTAGTGCGGTTGAAAGCCTCCACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCCCCTGCATGATGTCGTact                                                                                                                                     <  2:694152/142‑1 (MQ=255)
              gAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCtt                                                                                                                             >  1:178548/1‑149 (MQ=255)
                aaGTTTCGACATCCACGTACCGAAAGGTTACATCTATTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACATGATTCGCAACAAAAATAATCCGCTCTGATACTCCGTACGCCCTCCCGCATGAGAGCGTacggagtatcaga                                                                                                                           <  1:920022/149‑12 (MQ=255)
                  gTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTcc                                                                                                                               <  2:26698/143‑1 (MQ=255)
                   tttCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACCAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCt                                                                                                                              >  2:987849/1‑143 (MQ=255)
                     tCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCt                                                                                                                      <  1:48882/149‑1 (MQ=255)
                       gACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTca                                                                                                                    >  1:1173654/1‑149 (MQ=255)
                       gACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTca                                                                                                                    >  1:1130133/1‑149 (MQ=255)
                        aCATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTAc                                                                                                                         <  2:1035930/143‑1 (MQ=255)
                               cGTACCGAACGGTTACATCTACTGCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTaa                                                                                                            <  1:197791/149‑1 (MQ=255)
                                 tACCGGAAGGTTACATGTACTTCGCGATGTTTTTCTCTATTGCGGGTGAAAGCGTCAACTTGAGTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTACTTTGTCCCTCACAGt                                                                                                                <  2:20496/143‑1 (MQ=255)
                                   ccGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAtt                                                                                                        <  1:775651/149‑1 (MQ=255)
                                      aaaGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTc                                                                                                     <  1:472180/149‑1 (MQ=255)
                                      aaaGGTTACATCTACTTCGCGATGTTCTTCTCGATGGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTc                                                                                                     <  1:1121113/149‑1 (MQ=255)
                                         ggTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCCCCCCTTTTACCCTCACAGTTTAAGAtt                                                                                                        >  2:150222/1‑143 (MQ=255)
                                          gTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGc                                                                                                 <  1:1038007/149‑1 (MQ=255)
                                           ttACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGACTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCATACTCACTCCTTTTACCCTCAACGATTAAGAtttt                                                                                                      >  2:86530/1‑143 (MQ=255)
                                             aCATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAGAAAGAATCCGCTCTGATACTCCGTCCGCTCTCCTGCGGGAGGGCGTACTCTCTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCttt                                                                                              <  1:844227/149‑1 (MQ=255)
                                                  tACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGa                                                                                         <  1:39569/149‑1 (MQ=255)
                                                   aCTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACACTTTAAGATTTTCCTGCTTTCAAGat                                                                                        <  1:775984/149‑1 (MQ=255)
                                                        gcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATATTCCTGCTTTCAGGa                                                                                         >  2:454592/1‑143 (MQ=255)
                                                          gaggtgcttctCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCCCAAAGTTTGAGATTTTCCTGCTTTCAAGATATATAAc                                                                                 <  1:637495/143‑1 (MQ=255)
                                                           aTGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGatat                                                                                      <  2:846331/143‑1 (MQ=255)
                                                                cttctCTGTTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGCTACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTTCCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAAc                                                                                 <  2:821916/143‑1 (MQ=255)
                                                                  tctcTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTTTGATGCTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCCAGATATATAACGTCGGttt                                                                         <  1:946165/149‑1 (MQ=255)
                                                                      tATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGt                                                                           >  2:959506/1‑143 (MQ=255)
                                                                      caTTGCGGTTGAACGCCTCAACGTGATTCGCAACAAACAGAATCCGCTCCGATCCTCCGTCCGCTCTCCTGCGCGAGGGCGTACTCACTCCTTTTTCCCTCACCGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGt                                                                           <  2:550458/142‑1 (MQ=255)
                                                                       aTTGCGGTTGAAAGCCTCAACTTGAGTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACACTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTAACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGtt                                                                          <  2:311472/143‑1 (MQ=255)
                                                                        ttGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGttt                                                                         <  2:1116571/143‑1 (MQ=255)
                                                                        tggCGGGTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGCTTAAGATTTTCCTGCTTTCAAGATATATACCGTCGGttt                                                                         >  2:661024/3‑143 (MQ=255)
                                                                         tGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATCCTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAGGATATATAACGTCGGTTta                                                                        <  2:1106615/143‑1 (MQ=255)
                                                                           cGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTtata                                                                      >  2:765685/1‑143 (MQ=255)
                                                                           cGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGa                                                                >  1:77917/1‑149 (MQ=255)
                                                                             gTTGAAATCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCGCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGCTATATAACGTCGGTTTATaaa                                                                    <  2:958806/143‑1 (MQ=255)
                                                                             gTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATaaa                                                                    >  2:1084070/1‑143 (MQ=255)
                                                                                  acgCCTCAACTTGATTCGCAAAAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATAGAACGTCGGTTTATAAACAGAc                                                               <  2:1098020/141‑1 (MQ=255)
                                                                                     ccTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACCGACTATTATCAt                                                      >  1:876329/1‑149 (MQ=255)
                                                                                      cTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAta                                                     <  1:1066000/149‑1 (MQ=255)
                                                                                         aaCTTGATTCGCAACAAAAAGAATCCGCTCTGCTACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATc                                                        <  2:936672/143‑1 (MQ=255)
                                                                                         aaCTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggt                                                  <  1:1226223/149‑1 (MQ=255)
                                                                                           cTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggtag                                                >  1:436676/1‑149 (MQ=255)
                                                                                            ttGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAta                                                     >  2:668686/1‑143 (MQ=255)
                                                                                                 tCGCAACAAGAAGAATCCGCTCTGATACTCCGTACGCTCTCCTACGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggtag                                                >  2:587985/1‑143 (MQ=255)
                                                                                                 tCGCAACAAAAAGACTCCGCTCTGATACTCCGTTCGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTCAGATTTTCCTGCTCTCAAGATATATAACGTCGGTTTAGAAACAGACTATTATCATAGGTAGGCCaaa                                          <  1:792454/149‑1 (MQ=255)
                                                                                                  cGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTCAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAAt                                         <  1:1218960/149‑1 (MQ=255)
                                                                                                  cGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTCTATAAACAGACTATTATCATAGGTAGGCCAAAt                                         <  1:951956/149‑1 (MQ=255)
                                                                                                  cGCAACAAAAAGAATCCGCTCTGATACTCCATACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAAt                                         >  1:673995/1‑149 (MQ=255)
                                                                                                    caacaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGa                                       >  1:1234334/1‑149 (MQ=255)
                                                                                                     aacaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACAATTATCATAGGTAGGCCAAATGAg                                      <  1:969812/149‑1 (MQ=255)
                                                                                                         aaaaGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATa                                  >  1:1189724/1‑149 (MQ=255)
                                                                                                          aaaGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGa                                       <  2:761750/143‑1 (MQ=255)
                                                                                                                tCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAGCAGACTATTATCATAGGTAGGCCAAATGAGGATaa                                 >  2:1011988/1‑143 (MQ=255)
                                                                                                                     tctGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGaaaa                      >  1:998143/1‑149 (MQ=255)
                                                                                                                           cgtCCGTACGCCCTCCTGAGGGAGGTCGTACCCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTGCAAGATATATAACGTCGGTTTATAAACAGACCATTATCATAGGTAGGCCAAATGAGGATAACCGGATGaaaa                      <  2:358886/141‑1 (MQ=255)
                                                                                                                           aCTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATggg                >  1:344874/1‑149 (MQ=255)
                                                                                                                             tCCGTACGCTCTCATGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAACCAGACTATTATCATAGGTAGGTCAAATGAGGATAACCGGATGAAAAAATGGGCa              >  1:888574/1‑149 (MQ=255)
                                                                                                                              ccGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAAt                   <  2:1091094/143‑1 (MQ=255)
                                                                                                                               cGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATGGGTAGGCCAAATGAGGATAACCGGATGAAAAAATg                  >  2:167196/1‑143 (MQ=255)
                                                                                                                                 tACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATggg                <  2:224522/143‑1 (MQ=255)
                                                                                                                                 tACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATggg                >  2:874814/1‑143 (MQ=255)
                                                                                                                                 tACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACAGGATGAAAAAATggg                >  2:642262/1‑143 (MQ=255)
                                                                                                                                      tcggcTGGGGGAGGGCGTACTGCCTCCGTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGGAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGta           <  2:430569/139‑1 (MQ=255)
                                                                                                                                         ccTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCt  >  1:1257234/1‑149 (MQ=255)
                                                                                                                                         ccTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCt  >  1:1230710/1‑149 (MQ=255)
                                                                                                                                          cTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAAGTAGGCCAAATGAGGATAACCAGATGAAAAAATGGGCAAtaataa       >  2:320846/1‑143 (MQ=255)
                                                                                                                                           tgggggAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTaataat      <  2:730882/140‑1 (MQ=255)
                                                                                                                                           tGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTaataat      <  2:212636/143‑1 (MQ=255)
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TTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCT  >  CP009273/2964371‑2964656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑