Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,964,518 G→A 4.9% intergenic (+26/‑112) ygdQ → / → ygdR UPF0053 family inner membrane protein/DUF903 family verified lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,964,5180GA 4.9% 240.2 / 2.2 82intergenic (+26/‑112)ygdQ/ygdRUPF0053 family inner membrane protein/DUF903 family verified lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (36/42);  new base A (2/2);  total (38/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.37e-01

TACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGG  >  CP009273/2964372‑2964664
                                                                                                                                                  |                                                                                                                                                  
tACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGc                                                                                                                                                  >  1:1023209/1‑149 (MQ=255)
tACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGc                                                                                                                                                  >  1:935861/1‑149 (MQ=255)
   ccTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTa                                                                                                                                               >  1:801482/1‑149 (MQ=255)
   ccTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCTGCGGGAGGGCGTa                                                                                                                                               >  1:216013/1‑149 (MQ=255)
   ccTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTa                                                                                                                                               <  1:863848/149‑1 (MQ=255)
    cTGCTTCTGGAAGGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTGCTTCTCTATTGCTGGTGAAAGCCTCAACGTGATTCGCAGCAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCgggggg                                                                                                                                                    >  2:391649/1‑143 (MQ=255)
    cTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTCATTCGCGGTGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAAGCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAgg                                                                                                                                                    >  2:561016/1‑143 (MQ=255)
     ctaTTCTGGAGAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTCCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTact                                                                                                                                             <  1:472067/147‑1 (MQ=255)
       aTTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATTTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCg                                                                                                                                                 <  2:149899/143‑1 (MQ=255)
       aTTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCg                                                                                                                                                 <  2:385781/143‑1 (MQ=255)
       aTTCTGGAAAGTTCCGACATCCACGTACCGAAAGGTTACATCTACTGCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactca                                                                                                                                           <  1:1169135/149‑1 (MQ=255)
        ttCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGCGGGCGt                                                                                                                                                >  2:182608/1‑143 (MQ=255)
        ttCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactcac                                                                                                                                          >  1:557103/1‑149 (MQ=255)
           cggAAAGTCTCGACATCCACCGACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTAGTGCGGTTGAAAGCCTCCACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCCCCTGCATGATGTCGTact                                                                                                                                             <  2:694152/142‑1 (MQ=255)
             gAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCtt                                                                                                                                     >  1:178548/1‑149 (MQ=255)
               aaGTTTCGACATCCACGTACCGAAAGGTTACATCTATTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACATGATTCGCAACAAAAATAATCCGCTCTGATACTCCGTACGCCCTCCCGCATGAGAGCGTacggagtatcaga                                                                                                                                   <  1:920022/149‑12 (MQ=255)
                 gTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTcc                                                                                                                                       <  2:26698/143‑1 (MQ=255)
                  tttCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACCAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCt                                                                                                                                      >  2:987849/1‑143 (MQ=255)
                    tCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCt                                                                                                                              <  1:48882/149‑1 (MQ=255)
                      gACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTca                                                                                                                            >  1:1173654/1‑149 (MQ=255)
                      gACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTca                                                                                                                            >  1:1130133/1‑149 (MQ=255)
                       aCATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTAc                                                                                                                                 <  2:1035930/143‑1 (MQ=255)
                              cGTACCGAACGGTTACATCTACTGCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTaa                                                                                                                    <  1:197791/149‑1 (MQ=255)
                                tACCGGAAGGTTACATGTACTTCGCGATGTTTTTCTCTATTGCGGGTGAAAGCGTCAACTTGAGTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTACTTTGTCCCTCACAGt                                                                                                                        <  2:20496/143‑1 (MQ=255)
                                  ccGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAtt                                                                                                                <  1:775651/149‑1 (MQ=255)
                                     aaaGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTc                                                                                                             <  1:472180/149‑1 (MQ=255)
                                     aaaGGTTACATCTACTTCGCGATGTTCTTCTCGATGGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTc                                                                                                             <  1:1121113/149‑1 (MQ=255)
                                        ggTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCCCCCCTTTTACCCTCACAGTTTAAGAtt                                                                                                                >  2:150222/1‑143 (MQ=255)
                                         gTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGc                                                                                                         <  1:1038007/149‑1 (MQ=255)
                                          ttACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGACTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCATACTCACTCCTTTTACCCTCAACGATTAAGAtttt                                                                                                              >  2:86530/1‑143 (MQ=255)
                                            aCATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAGAAAGAATCCGCTCTGATACTCCGTCCGCTCTCCTGCGGGAGGGCGTACTCTCTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCttt                                                                                                      <  1:844227/149‑1 (MQ=255)
                                                 tACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGa                                                                                                 <  1:39569/149‑1 (MQ=255)
                                                  aCTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACACTTTAAGATTTTCCTGCTTTCAAGat                                                                                                <  1:775984/149‑1 (MQ=255)
                                                       gcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATATTCCTGCTTTCAGGa                                                                                                 >  2:454592/1‑143 (MQ=255)
                                                         gaggtgcttctCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCCCAAAGTTTGAGATTTTCCTGCTTTCAAGATATATAAc                                                                                         <  1:637495/143‑1 (MQ=255)
                                                          aTGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGatat                                                                                              <  2:846331/143‑1 (MQ=255)
                                                               cttctCTGTTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGCTACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTTCCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAAc                                                                                         <  2:821916/143‑1 (MQ=255)
                                                                 tctcTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTTTGATGCTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCCAGATATATAACGTCGGttt                                                                                 <  1:946165/149‑1 (MQ=255)
                                                                     tATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGt                                                                                   >  2:959506/1‑143 (MQ=255)
                                                                     caTTGCGGTTGAACGCCTCAACGTGATTCGCAACAAACAGAATCCGCTCCGATCCTCCGTCCGCTCTCCTGCGCGAGGGCGTACTCACTCCTTTTTCCCTCACCGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGt                                                                                   <  2:550458/142‑1 (MQ=255)
                                                                      aTTGCGGTTGAAAGCCTCAACTTGAGTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACACTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTAACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGtt                                                                                  <  2:311472/143‑1 (MQ=255)
                                                                       ttGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGttt                                                                                 <  2:1116571/143‑1 (MQ=255)
                                                                       tggCGGGTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGCTTAAGATTTTCCTGCTTTCAAGATATATACCGTCGGttt                                                                                 >  2:661024/3‑143 (MQ=255)
                                                                        tGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATCCTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAGGATATATAACGTCGGTTta                                                                                <  2:1106615/143‑1 (MQ=255)
                                                                          cGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTtata                                                                              >  2:765685/1‑143 (MQ=255)
                                                                          cGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGa                                                                        >  1:77917/1‑149 (MQ=255)
                                                                            gTTGAAATCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCGCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGCTATATAACGTCGGTTTATaaa                                                                            <  2:958806/143‑1 (MQ=255)
                                                                            gTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATaaa                                                                            >  2:1084070/1‑143 (MQ=255)
                                                                                 acgCCTCAACTTGATTCGCAAAAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATAGAACGTCGGTTTATAAACAGAc                                                                       <  2:1098020/141‑1 (MQ=255)
                                                                                    ccTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACCGACTATTATCAt                                                              >  1:876329/1‑149 (MQ=255)
                                                                                     cTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAta                                                             <  1:1066000/149‑1 (MQ=255)
                                                                                        aaCTTGATTCGCAACAAAAAGAATCCGCTCTGCTACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATc                                                                <  2:936672/143‑1 (MQ=255)
                                                                                        aaCTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggt                                                          <  1:1226223/149‑1 (MQ=255)
                                                                                          cTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggtag                                                        >  1:436676/1‑149 (MQ=255)
                                                                                           ttGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAta                                                             >  2:668686/1‑143 (MQ=255)
                                                                                                tCGCAACAAGAAGAATCCGCTCTGATACTCCGTACGCTCTCCTACGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggtag                                                        >  2:587985/1‑143 (MQ=255)
                                                                                                tCGCAACAAAAAGACTCCGCTCTGATACTCCGTTCGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTCAGATTTTCCTGCTCTCAAGATATATAACGTCGGTTTAGAAACAGACTATTATCATAGGTAGGCCaaa                                                  <  1:792454/149‑1 (MQ=255)
                                                                                                 cGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTCAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAAt                                                 <  1:1218960/149‑1 (MQ=255)
                                                                                                 cGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTCTATAAACAGACTATTATCATAGGTAGGCCAAAt                                                 <  1:951956/149‑1 (MQ=255)
                                                                                                 cGCAACAAAAAGAATCCGCTCTGATACTCCATACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAAt                                                 >  1:673995/1‑149 (MQ=255)
                                                                                                   caacaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGa                                               >  1:1234334/1‑149 (MQ=255)
                                                                                                    aacaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACAATTATCATAGGTAGGCCAAATGAg                                              <  1:969812/149‑1 (MQ=255)
                                                                                                        aaaaGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATa                                          >  1:1189724/1‑149 (MQ=255)
                                                                                                         aaaGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGa                                               <  2:761750/143‑1 (MQ=255)
                                                                                                               tCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAGCAGACTATTATCATAGGTAGGCCAAATGAGGATaa                                         >  2:1011988/1‑143 (MQ=255)
                                                                                                                    tctGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGaaaa                              >  1:998143/1‑149 (MQ=255)
                                                                                                                          cgtCCGTACGCCCTCCTGAGGGAGGTCGTACCCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTGCAAGATATATAACGTCGGTTTATAAACAGACCATTATCATAGGTAGGCCAAATGAGGATAACCGGATGaaaa                              <  2:358886/141‑1 (MQ=255)
                                                                                                                          aCTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATggg                        >  1:344874/1‑149 (MQ=255)
                                                                                                                            tCCGTACGCTCTCATGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAACCAGACTATTATCATAGGTAGGTCAAATGAGGATAACCGGATGAAAAAATGGGCa                      >  1:888574/1‑149 (MQ=255)
                                                                                                                             ccGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAAt                           <  2:1091094/143‑1 (MQ=255)
                                                                                                                              cGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATGGGTAGGCCAAATGAGGATAACCGGATGAAAAAATg                          >  2:167196/1‑143 (MQ=255)
                                                                                                                                tACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATggg                        <  2:224522/143‑1 (MQ=255)
                                                                                                                                tACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATggg                        >  2:874814/1‑143 (MQ=255)
                                                                                                                                tACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACAGGATGAAAAAATggg                        >  2:642262/1‑143 (MQ=255)
                                                                                                                                     tcggcTGGGGGAGGGCGTACTGCCTCCGTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGGAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGta                   <  2:430569/139‑1 (MQ=255)
                                                                                                                                        ccTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCt          >  1:1230710/1‑149 (MQ=255)
                                                                                                                                        ccTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCt          >  1:1257234/1‑149 (MQ=255)
                                                                                                                                         cTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAAGTAGGCCAAATGAGGATAACCAGATGAAAAAATGGGCAAtaataa               >  2:320846/1‑143 (MQ=255)
                                                                                                                                          tgggggAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTaataat              <  2:730882/140‑1 (MQ=255)
                                                                                                                                          tGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTaataat              <  2:212636/143‑1 (MQ=255)
                                                                                                                                              ggggggCGTACTCCCTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCt          <  2:445333/140‑1 (MQ=255)
                                                                                                                                              ggAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTc    >  1:931953/1‑149 (MQ=255)
                                                                                                                                               gAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTg         <  2:377271/143‑1 (MQ=255)
                                                                                                                                               gAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTg         >  2:784242/1‑143 (MQ=255)
                                                                                                                                                aGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATTTAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCgg  >  1:1014431/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGG  >  CP009273/2964372‑2964664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: