Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,019,631	G→T	41.1%	M169I (ATG→ATT)	guaD →	guanine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,019,631	0	G	T	41.1%	51.9 / 90.2	92	M169I (ATG→ATT)	guaD	guanine deaminase
Reads supporting (aligned to +/- strand):  ref base G (31/23);  new base T (23/15);  total (54/38)
Fisher's exact test for biased strand distribution p-value = 8.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01

AATGTCGGCGTTCTTCATCAAGCAGCTTTTACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGCTTCGCCCCGACCTCATCTCCTGAAC  >  CP009273/3019487‑3019776                                                                                                                                                 |                                                                                                                                                  aaTGTCCGCGTTCTTCCTCAAGCAGCTTTTACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATgg                                                                                                                                               <  1:903850/149‑1 (MQ=255)    gTCGGCGTTCTTCATCAAGCAGCTTTTACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGAcc                                                                                                                                            >  1:977080/1‑149 (MQ=255)           ttcttcATCAAGCAGCTTTTACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGACCg                                                                                                                                           >  2:923771/1‑143 (MQ=255)             cttcATCAAGCAGCTTTTACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCTTCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGAGGATGGACCGCa                                                                                                                                         >  2:842678/1‑143 (MQ=255)              ttcATCAAGCAACTTTTACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGACGGCAACGCAcc                                                                                                                                  >  1:1213505/1‑149 (MQ=255)                catcaAGCAGCTTTTACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCGAGTCATAACAATATGCGTATGATTGCCGGTAAGGTGATGATGGACCTCAACGAACCgg                                                                                                                                >  1:306014/1‑149 (MQ=255)                catcaAGCAGCTTTTACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGACCGCCACGCACCgg                                                                                                                                >  1:445567/1‑149 (MQ=255)                catcaAGCAGCTTTTACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACg                                                                                                                                      >  2:375250/1‑143 (MQ=255)                  tcaAGCGGCTTTTACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGCa                                                                                                                                    <  2:1028347/143‑1 (MQ=255)                    aaGCAGCTTTTACGTAACGGAACCACCACGTCGCTGGTGTTTGGCACTGTTCATCCGCAATACGTTCAAGCACTGTTTGAAGCCCCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGAcggacaagcacc                                                                                                                                  >  2:59781/1‑132 (MQ=255)                    aaGCAGCTTTTACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTa                                                                                                                            <  1:383357/149‑1 (MQ=255)                     agcagcTTTTACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCg                                                                                                                                 <  2:875457/143‑1 (MQ=255)                      gcagcTTTTACGTATCGGAACCACCACGGCGCTGGTATTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGCACCGCAATGCACCGGATTATc                                                                                                                          <  1:919597/149‑1 (MQ=255)                      gcagcTTTTACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCgg                                                                                                                                >  2:920198/1‑143 (MQ=255)                       cagcTTTTACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCt                                                                                                                         >  1:1113949/1‑149 (MQ=255)                           ttttACGTAACGGAACCACCACGGTGCTGGTGTTTGGCACTCTTCATCCGCAATCTGTTGATGCGCTGTCTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAACGTGATGATGGACCGCAACGCACCGGATTAt                                                                                                                           <  2:950474/143‑1 (MQ=255)                           ttttACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACAGCAACGCACCGGATTAt                                                                                                                           >  2:180908/1‑143 (MQ=255)                           ttttACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTAt                                                                                                                           <  2:550879/143‑1 (MQ=255)                            tttACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCg                                                                                                                    >  1:1118645/1‑149 (MQ=255)                             ttACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCGa                                                                                                                   >  1:1270725/1‑149 (MQ=255)                                 gTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACt                                                                                                               >  1:972547/1‑149 (MQ=255)                                 gTAACGGAACCACCACGGCGCTGGAGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTCCCGGTAAGGTGATTTTGGACCGCAACGGACCGGATTATCAGATAGACACt                                                                                                               >  1:448910/1‑149 (MQ=255)                                 gTAACGAAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTc                                                                                                                     >  2:1088982/1‑143 (MQ=255)                                     cGGAACCACCACGGCGATGGTGTTTGGCAGTGTTCATCCGCAATCTGTTGATGAGCAGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTAACGATAAGTTGATTATGGACCGCAACGCACCGGATTATCGACTAGACAATGGCg                                                                                                           >  1:818471/1‑149 (MQ=255)                                      ggAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTCGATGCGCGGTTTGAAGCCGCCAGTCATATCAATATGCGTACGATTCCCGGAAAGGTGATGAGGGACCGCAACACACCGGATTATCGGCTGGacac                                                                                                                >  2:559000/1‑143 (MQ=255)                                            accacGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGGTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGCTGATGGACGGCAACGCACCGGATTATCTGGTCGACCCTGCCGa                                                                                                          >  2:471229/1‑143 (MQ=255)                                              cacGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGGAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGaaa                                                                                                        >  2:601344/1‑143 (MQ=255)                                              cacGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACGGCACCGCACCGGATTATCCGGCCGACACTGCCGaaa                                                                                                        >  2:603309/1‑143 (MQ=255)                                                   cgcTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCACCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTACTCGACACTGCCGAAAGCAGCTATCAc                                                                                             >  1:741370/1‑149 (MQ=255)                                                    gcTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAGGCAGCTATAAcc                                                                                            >  1:192781/1‑149 (MQ=255)                                                    gcTGGTGGTGGGCACTGTTCATCCGCAAGCGGTGGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCGGGTAAGGTGAGTTTGTTTCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCAcc                                                                                            <  1:577094/149‑1 (MQ=255)                                                    catggatgttGGCACTGTGCATCCGCAATCTGGTGATGCGTTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAAAGCACCGGATTATCTGCCCGACACTGCCGAAAGCAGCt                                                                                                  <  2:808641/135‑1 (MQ=255)                                                          gtTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGGATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACcaaagca                                                                                      >  1:891149/1‑149 (MQ=255)                                                           tttGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCACCGCACCGGACTATCTGCTCGACACTGCCGACAGCAGCTATCACca                                                                                           >  2:974353/1‑143 (MQ=255)                                                             tGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTACCACcaaa                                                                                         >  2:429081/1‑143 (MQ=255)                                                                 aCTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTCTCACcaaaacaa                                                                                     >  2:262568/1‑143 (MQ=255)                                                                 aCTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACt                                                                               <  1:205219/149‑1 (MQ=255)                                                                   tGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATGTGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAGGAACtac                                                                             >  1:371682/1‑147 (MQ=255)                                                                    gTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGTCACTGCCGAAAGCAGCTATGACCAAAGCAAAGa                                                                                  >  2:291987/1‑143 (MQ=255)                                                                       cATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCCGCTATCACcaaaacaaacacct                                                                               >  2:438539/1‑138 (MQ=255)                                                                        aTCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTACCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGaa                                                                        <  1:1047672/149‑1 (MQ=255)                                                                         tCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCGACGCACCGGATTATCTGCTGTGCACTGCCGAAAGCAGCTATCACCAAAGCAAAAAACTGa                                                                             >  2:364554/1‑143 (MQ=255)                                                                         tCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAAc                                                                       >  1:466494/1‑149 (MQ=255)                                                                          ccGCAATCTGTTGATGCGTTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACg                                                                      <  1:1200852/149‑1 (MQ=255)                                                                          ccGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACGGATCGAACg                                                                      >  1:1031441/1‑149 (MQ=255)                                                                           cGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATc                                                                           <  2:980553/143‑1 (MQ=255)                                                                           cGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATACGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGc                                                                     <  1:542525/149‑1 (MQ=255)                                                                            gCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCGGAAAGCTGCCAGCACCAAAGCAAAGAACCGAACg                                                                          >  2:56974/1‑143 (MQ=255)                                                                              aaTCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCCCCAAAGCAAAAAACTGATCGAACGCTgg                                                                  >  1:1145231/1‑149 (MQ=255)                                                                               aTCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCCCCAAAGCCAAGAACTGATCGAACGCTGGc                                                                 >  1:311239/1‑149 (MQ=255)                                                                               aTCTGTTGATGCGCCGTTTGAAGCCGCCAGTTCTATCAATATGCGTATGATCGCCGGTAAGGTGATTATGGACCGCACCGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGc                                                                 <  1:34836/149‑1 (MQ=255)                                                                                 cTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGcac                                                               <  1:1006863/149‑1 (MQ=255)                                                                                   gTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTg                                                                   <  2:567672/143‑1 (MQ=255)                                                                                    ttGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTgg                                                                  <  2:1147466/143‑1 (MQ=255)                                                                                       aTGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGcac                                                               <  2:39458/143‑1 (MQ=255)                                                                                         gcgcTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCGGGCaaaa                                                             >  2:907913/1‑143 (MQ=255)                                                                                         gcgcTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATgg                                                       >  1:202252/1‑149 (MQ=255)                                                                                          cgcTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGgt                                                      >  1:1151043/1‑149 (MQ=255)                                                                                          cgcTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGgt                                                      >  1:435697/1‑149 (MQ=255)                                                                                              gTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGgtcgtc                                                  <  1:704936/149‑1 (MQ=255)                                                                                               tgtGAAGCCGCCAGTCATATCAATAGGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCt                                                 <  1:1064571/147‑1 (MQ=255)                                                                                                  gAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGgtcg                                                    <  2:737406/143‑1 (MQ=255)                                                                                                  gAAGCCGCCAGTCAGATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGgtcg                                                    <  2:27357/143‑1 (MQ=255)                                                                                                    aGCCGCCAGCCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGAATTATCTGCTCGACACTGCCGAAAGCAGATAAAAAAAAAAAAAAGAACTGATCGAACGCTGGCACAAAAATGgtcgtc                                                  <  2:584666/143‑1 (MQ=255)                                                                                                     gcggccAGTGGTGTCAGTGTGCGTTTGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATGTGCTCGACACTGCCGAAAGCAGCTATCACCAATGCCAAGAACTGATCGAACGCTGGCACAAAAATGGGCGTCt                                                 <  2:545789/140‑1 (MQ=255)                                                                                                      ccgccAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGGACTGATCGAACGCTGGCACAAAAATGGTCGTCTg                                                <  2:375243/143‑1 (MQ=255)                                                                                                        gccAGTCATCTCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGcc                                              >  2:661623/1‑142 (MQ=255)                                                                                                        gccAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTctatcc                                        >  1:1024240/1‑147 (MQ=255)                                                                                                        gccAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGc                                        >  1:1121587/1‑149 (MQ=255)                                                                                                          cAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCtat                                            <  2:798758/143‑1 (MQ=255)                                                                                                             tCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATTGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATg                                         <  2:890411/143‑1 (MQ=255)                                                                                                             tCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGCCCGCAACGCACCGGATTATCTGCTCGACACTGTCGAAAAAAAAAAAAAACAAAAAAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATg                                         <  2:921541/143‑1 (MQ=255)                                                                                                             tCATATCAATATGCGTATGATTGACGGTAAGGTGATGATGGCCCGCAACGCACCGGATTATGTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATg                                         <  2:681084/143‑1 (MQ=255)                                                                                                                tatCAATATGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGAACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGa                                      <  2:524530/143‑1 (MQ=255)                                                                                                                  tCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATACGATTACGcaa                              >  1:543238/1‑147 (MQ=255)                                                                                                                       atGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGCtt                         >  1:784958/1‑149 (MQ=255)                                                                                                                        tGCGTATGATTGCCGGTAAGGGGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCa                              <  2:513758/143‑1 (MQ=255)                                                                                                                         gCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGAACCGGATTATCGGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGTTCGTCTGCTATATGCGATTACGCCAc                             <  2:924974/143‑1 (MQ=255)                                                                                                                         gCGTATGATTGCCGGCACGGTGCTGGAGGACCGGAATGCGCCGGACCTTCTTCTCGTCACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGCTTCg                       <  1:297922/149‑1 (MQ=255)                                                                                                                            tATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCCACGCAAAGAACTGATCGAACGCTGGCTCAGAAATGGTCGCCTGCTGTATGCGGTTACGCCCCGcc                          >  2:152308/1‑142 (MQ=255)                                                                                                                            tATGATTGCCGGTAAGGTGATGATTGACAGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATGACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGCTTCGccc                    >  1:972668/1‑149 (MQ=255)                                                                                                                            tATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCTAACGCTGGCACAAAAATGGTCTTCTGCTATATGCGATTACGCCACGCt                          >  2:617466/1‑143 (MQ=255)                                                                                                                             aTGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGCTTCGcccc                   >  1:1066316/1‑149 (MQ=255)                                                                                                                             aTGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGCTTCGcccc                   >  1:665490/1‑149 (MQ=255)                                                                                                                                 ttGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGCTTCGcc                     >  2:789470/1‑143 (MQ=255)                                                                                                                                  tGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACACAAATGGTCGTCTGCTATATGCGATTACGCCACGCTTCGccc                    >  2:234666/1‑143 (MQ=255)                                                                                                                                  tGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCGGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGCTTCGCCCCGACCt              >  1:946010/1‑149 (MQ=255)                                                                                                                                      ggTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGCTTCGCCCCGAc                <  2:930937/143‑1 (MQ=255)                                                                                                                                        tAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGCTTCGCCCCGACCTCAtctc        <  1:163123/149‑1 (MQ=255)                                                                                                                                         caGGTGACGATGGACCTCAACCCACCGGATTATCTGCCCGCCACTGCCGAAAGCAGCTATCACCCAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTTCGCCACGCTTCGCCCCGACCTc             <  2:530765/142‑1 (MQ=255)                                                                                                                                          aGGTGATTGTGGACCGCAACGCACCGTATTATCTGCTCGACACTGGCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGATCGCTGGCACAAATATGGTCGTCTGCTATATGCGATTACGCCACGCTTCGCCCCGACCTCa            <  2:632458/143‑1 (MQ=255)                                                                                                                                            gTGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGCCAGCAGCTATCACCAAAGCCAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACCCTCCCCCCCGCCCTCAta          >  2:1023143/1‑142 (MQ=255)                                                                                                                                             tgatgaTGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGCTTCGCCCCGACCTCATCTCCTGaa   <  1:67077/149‑1 (MQ=255)                                                                                                                                              gatgatGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGCTTCGCCCCGACCTCATCTCCTGCAc  >  1:119507/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                                  AATGTCGGCGTTCTTCATCAAGCAGCTTTTACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGCTTCGCCCCGACCTCATCTCCTGAAC  >  CP009273/3019487‑3019776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑