Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,029,079 C→T 39.9% A188T (GCT→ACT)  prfB ← peptide chain release factor RF‑2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,029,0790CT39.9% 68.8 / 103.9 113A188T (GCT→ACT) prfBpeptide chain release factor RF‑2
Reads supporting (aligned to +/- strand):  ref base C (35/32);  new base T (25/19);  total (60/53)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 115 total aligned reads displayed.

GCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTTCCGT  >  CP009273/3028931‑3029226
                                                                                                                                                    |                                                                                                                                                   
gccgGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGc                                                                                                                                                     <  1:401792/149‑1 (MQ=255)
gccgGGTGGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGc                                                                                                                                                     <  1:383278/149‑1 (MQ=255)
       tGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTTTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCCGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCg                                                                                                                                              >  1:661098/1‑149 (MQ=255)
         aTTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGcc                                                                                                                                            <  1:702374/149‑1 (MQ=255)
         aTTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGcc                                                                                                                                            <  1:1244857/149‑1 (MQ=255)
          tttCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCg                                                                                                                                           >  1:82457/1‑149 (MQ=255)
          tttCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTGCTGTACGCAGCGAGCCGGAAGTGTAATCGCCg                                                                                                                                           >  1:645780/1‑149 (MQ=255)
          tttCGATAGCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCg                                                                                                                                           <  1:1013161/149‑1 (MQ=255)
              gATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGgaga                                                                                                                                       >  1:289498/1‑149 (MQ=255)
              gATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACACACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGgaga                                                                                                                                       >  1:1144649/1‑149 (MQ=255)
               atatcaatatcaTCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTGAGCGTAATCGCCGGAGAt                                                                                                                                      >  1:1266560/1‑149 (MQ=255)
               atatcaatatcaTCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGAt                                                                                                                                      >  1:1151460/1‑149 (MQ=255)
                 atcaatatcaTCATCAACTTCCGGATAAACAAACGCGGGGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGATTCTGTAGGCCGCCGGCCGTCAGTGGAACCGCCGGAGAgtt                                                                                                                                    >  1:563762/1‑149 (MQ=255)
                 atcaatatcaTCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCGGCCGTAAGCGTAATCGCCgg                                                                                                                                          >  2:1133881/1‑143 (MQ=255)
                 atcaatatcaTCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCACCCGTAAGTGTAATCGCCGGAGAttt                                                                                                                                    <  1:777358/149‑1 (MQ=255)
                  tcaatatcaTCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGAtttt                                                                                                                                   >  1:1099150/1‑149 (MQ=255)
                  gcaatatcaTCATCAATTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGAtttt                                                                                                                                   <  1:94147/148‑1 (MQ=255)
                     atatcaTCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGCCGGTCATCGTTGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGAt                                                                                                                                <  1:303174/149‑1 (MQ=255)
                     atatcaTCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGCCCCCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGAt                                                                                                                                <  1:1225067/149‑1 (MQ=255)
                        tcatcatcaACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGAtttt                                                                                                                                   >  2:107802/1‑143 (MQ=255)
                        tcatcatcaACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAAACGCCGGAGATTTTGATCGt                                                                                                                             >  1:1102309/1‑149 (MQ=255)
                         catcatcaACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATAGCCGGAGATTTTg                                                                                                                                  >  2:380305/1‑143 (MQ=255)
                          attatcaACTTCGGGATAAGCCAACGCGGGGCGGAACGACGGGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCa                                                                                                                           <  1:45228/146‑1 (MQ=255)
                           tcatcaACTTCCGGATAAACAACCGCGGAGCCGAACGACGTGGGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCAc                                                                                                                          <  1:354033/149‑1 (MQ=255)
                           tcatcaACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTGGGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGAt                                                                                                                                <  2:1051923/143‑1 (MQ=255)
                           tcatcaACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGAt                                                                                                                                >  2:896182/1‑143 (MQ=255)
                              tcaACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGGGTCAAACGGGCATTTACGCACCCGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTCAGCGTAATCGCCGGAACTTTTGacact                                                                                                                             >  2:283353/1‑139 (MQ=255)
                              tcaACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTCAGCGTACTCGCCGGCGATTTTGAACGt                                                                                                                             >  2:923300/1‑143 (MQ=255)
                              tcaACTTCAGGATAAACCAACGCGGCGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGa                                                                                                                       <  1:1083028/149‑1 (MQ=255)
                               caACGTCCGGATAAACAAACGCGTGGCTGAACGACGTGTGGCCACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTc                                                                                                                            <  2:1000977/143‑1 (MQ=255)
                                caCTTCCGGATAAACAAACCCCGAGCTGAACGACGTGCGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGTACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGAtt                                                                                                                     <  1:39488/148‑1 (MQ=255)
                                   ttCCGGATAAACAAACGCTGCGCTGAACGACGTGTGGCGAAGCCCGCCGTATTCAACCGGGAGTCTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTaa                                                                                                                  >  1:954141/1‑149 (MQ=255)
                                      cGGATAAACACACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATAc                                                                                                               <  1:855496/149‑1 (MQ=255)
                                         aTAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTGACGGGtttca                                                                                                                  >  2:388289/1‑141 (MQ=255)
                                         aTAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGGCAAACGGGCTTTCACGCACTAGGCTTTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCg                                                                                                            >  1:890632/1‑149 (MQ=255)
                                          tAAACAACCGCGGAGCTGAACGACGTGTGGCGACGTCCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTCTCATCGTCACGGATTTAATACCCGc                                                                                                           <  1:726846/149‑1 (MQ=255)
                                           aaacaaacGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAGGTGTAATCGCCGGAGATTTTGATCGCCACGGATTTAATACCCGcc                                                                                                          >  1:461451/1‑149 (MQ=255)
                                              caaacGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACGCGGCGGTGAGCGCCGGTTTGTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATAccc                                                                                                             >  2:63715/1‑143 (MQ=255)
                                              caaacGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAAATTTTGAACGTCACGGATTTAATAacc                                                                                                             >  2:1031199/1‑143 (MQ=255)
                                               aaacGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGGCCGGCGTTAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTACTACCCGCCACtt                                                                                                      >  1:301942/1‑149 (MQ=255)
                                                    cgGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCt                                                                                                 >  1:1276361/1‑149 (MQ=255)
                                                        gCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCAGGCACTTCAc                                                                                                   >  2:6976/1‑143 (MQ=255)
                                                         cTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCGGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTACTCGCCGGACATTTGGATCGTCCCGGATTTCAAACACGCGACTTCAcc                                                                                                  >  2:491560/1‑143 (MQ=255)
                                                         cTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGGCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAACAGCCGGAGATCTTGCTCGTCACGGACTTAATACTCACCACAACAcc                                                                                                  >  2:721324/1‑143 (MQ=255)
                                                          tGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAAACGCCGGAGATTTTGATCGTCACGGAATTAATACCCGCCCCTTCACCTTCCGAc                                                                                           >  1:820628/1‑149 (MQ=255)
                                                          ggAACGCGGTGTGGCGCCGACCGGCGGAGTCAAACGGGCTTTTACGCCCCAGGCGGTGACCGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGAc                                                                                           <  1:397847/148‑1 (MQ=255)
                                                            aaCGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACAGGGCGGGGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGGTCGTCACGGATTTAATACCCGCCACTTCACCTTc                                                                                               >  2:857866/1‑143 (MQ=255)
                                                            aaCGACGGGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCCGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTc                                                                                               <  2:588901/143‑1 (MQ=255)
                                                              cgacgTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCtt                                                                                       >  1:615541/1‑149 (MQ=255)
                                                                       gCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGAttt                                                                              >  1:55216/1‑149 (MQ=255)
                                                                        ggcctcccgccgGAGTCAACCGGGCTTTTACGCCCCAGGGGGTGAACGCCGGTTTCTGTACCCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGACCGTACAAGAGTTAATACCCGCCACTTCACCTTCCGACTCTTCgat                                                                                   <  2:194338/137‑1 (MQ=255)
                                                                         gacgacCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTGCTGTACGCAGCCAGCCGGAAGCGTAATCGCCGGAGATTTTGATCGGCCCGGATTTAATACCCGCCACTTCACCTGCCGACTCCTCgatg                                                                                  >  2:745158/1‑143 (MQ=255)
                                                                            gacCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTGCTGTACACAGCGAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCCCTTCACCTTCCGACTCTTCCattatt                                                                               >  2:744322/1‑143 (MQ=255)
                                                                              ccgccgGTGTCAAACGGGCTTGTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGtttt                                                                       <  1:936921/149‑1 (MQ=255)
                                                                               cgccgGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTg                                                                      >  1:858594/1‑149 (MQ=255)
                                                                                  cgGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGaaa                                                                   <  1:731499/149‑1 (MQ=255)
                                                                                       tCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACTCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCAcg                                                              <  1:244452/149‑1 (MQ=255)
                                                                                         aagcGGGCTTTTACGCTGCAGGCGGTGAACGCCGGGTTCTGTACGCAGCCAGCCGTGAGCGTAATCGCCGGGGTGTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGGTGATTTCAGTGTTGAAAc                                                                  >  2:727321/4‑143 (MQ=255)
                                                                                         aaaCGGGCTTTTACGCACCAGGGGGTGAACGCCGGTTTCTGTAGGCAGCCAGCCGTAAGCGGAATCGCCGGAGAGTTTGATCGTGCCGGATTTAATACCCGCCACTGCACCTTCCGACTCTTCGCTGATTTCAGTTTTGaacc                                                                  >  2:392768/1‑141 (MQ=255)
                                                                                         aaaCGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCAGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAAc                                                                  >  2:978465/1‑143 (MQ=255)
                                                                                         aaaCGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCAcgcg                                                            >  1:1086205/1‑149 (MQ=255)
                                                                                          aaCGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCCACTCTTAGATGATTTCAGATTTGAAACAACCCGa                                                           >  1:449169/1‑149 (MQ=255)
                                                                                              ggCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCt                                                       <  1:1168659/149‑1 (MQ=255)
                                                                                                 ttttACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGcc                                                    >  1:772494/1‑149 (MQ=255)
                                                                                                   ttACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTc                                                        <  2:967111/143‑1 (MQ=255)
                                                                                                   ttACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCa                                                  <  1:320823/149‑1 (MQ=255)
                                                                                                    caCGCACCAGGCGGTGAACGCCCGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGCCACGGATTTAACACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAg                                                 <  1:280357/148‑1 (MQ=255)
                                                                                                       gCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCAGGAGAGTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAgcgc                                              >  1:1222123/1‑149 (MQ=255)
                                                                                                        cgccAGGCGGTGAACGCCGGGTCGTGTGCGCGCCCAGCCGTAAGTGTACTCGCCGGAGATTTGGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGccc                                                   <  2:415728/141‑1 (MQ=255)
                                                                                                        cACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCa                                             <  1:1289898/149‑1 (MQ=255)
                                                                                                         aCCCGGCCGTGAACGCCGGTTTCTGTACGCAGCAAGCCGTAAGGGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAg                                            <  1:356984/149‑1 (MQ=255)
                                                                                                         aCCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGAAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTATTCGATGAATTCGGTTTTCAAACAACACGATTCTGCCCa                                                  >  2:315514/1‑143 (MQ=255)
                                                                                                          ccAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAg                                                 >  2:502820/1‑143 (MQ=255)
                                                                                                           ccggCGGTGGACGCCGGTTTCTGTACGCGGCCAGCCGTAAGTGTAAGCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGat                                          <  1:125103/147‑1 (MQ=255)
                                                                                                              gCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCa                                             >  2:1096021/1‑143 (MQ=255)
                                                                                                              gCGGTGAACGCCGGTTTCGGTGCGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGAGATACTGcacacagca                                             <  2:438871/143‑6 (MQ=255)
                                                                                                                  tGAACGCCGGTTTCTGTCCGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTTACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACg                                   <  1:675690/149‑1 (MQ=255)
                                                                                                                    aaCGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCt                                 <  1:119796/149‑1 (MQ=255)
                                                                                                                      cGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGCTGATTTCAGTTTTGAAACCACCGGCTTCTGACCAGCGCAggcacaga                                     >  2:1082236/1‑136 (MQ=255)
                                                                                                                      cGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCa                               >  1:765227/1‑149 (MQ=255)
                                                                                                                       gCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCaa                              >  1:1181399/1‑149 (MQ=255)
                                                                                                                       cccGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCaa                              <  1:42591/148‑1 (MQ=255)
                                                                                                                        ccGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAg                             <  1:1066833/149‑1 (MQ=255)
                                                                                                                         cGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCCCCGGAGATTTTGATCGTCACGGATTTAATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGc                                  <  2:384149/143‑1 (MQ=255)
                                                                                                                            tttCTGTACGCAGCCGGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCCCTTCACCTTCCGACGCTTCGATGATTTCGGTTTTGACACCACGCGATTCTGCCCAGCGCAGATTCATACGCTCAAGCATg                         >  1:198974/1‑149 (MQ=255)
                                                                                                                             ttCTGTGCGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTCCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCaa                              <  2:1014465/143‑1 (MQ=255)
                                                                                                                             ttCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGCGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGc                        >  1:634482/1‑149 (MQ=255)
                                                                                                                               cTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGGGTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTc                      >  1:898334/1‑149 (MQ=255)
                                                                                                                                ggTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCCCTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACCTACGCTCAAACa                           >  2:887196/2‑143 (MQ=255)
                                                                                                                                  gaCGCAGCCCGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCGAGCGCAGATACATACGCTCAAGCATGCTCGcc                   <  1:637061/148‑1 (MQ=255)
                                                                                                                                   aCGCAGCCAGCCGTAAGTGTAATAGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGAGTTCAGTTTTGAATCCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGc                        >  2:777757/1‑143 (MQ=255)
                                                                                                                                     gCAGCCAGCCGTAAGCGCAATCGCCAGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTAAACAGCATAGATACATACGCTCAAGCATGCTc                      <  2:565999/143‑1 (MQ=255)
                                                                                                                                      cagccaacCGTAAGTTTAATCCCCGCAGATTTCGACCCCAACGGATTTAACACCCGCCACTTCACCTCCCGACCCTTCCACGATTTCAGTTTTGAAACCACGCGATTCTGCCCAACGCAAATACATACGCTCAAGCATGCTCg                     <  2:164737/143‑1 (MQ=255)
                                                                                                                                         ccagccGTAAGGGTAATCGCCGGAGATTTTTATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCt            <  1:295175/149‑1 (MQ=255)
                                                                                                                                          cagccGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCa                 <  2:501035/143‑1 (MQ=255)
                                                                                                                                            gccGTAAGCGTAATCGCCGGAGATTTTGGTCGTCACGGTTTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCtgtg         <  1:564678/149‑1 (MQ=255)
                                                                                                                                               gTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCTCCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCt            <  2:882926/143‑1 (MQ=255)
                                                                                                                                                tAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCCGGCCtg           >  2:328788/1‑143 (MQ=255)
                                                                                                                                                 aaGCGTAATCGCCGTAGATTTGGATCGTCACGGAGTTAACACCCGCCACCTCTCCTTCCGCTTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCtgt          <  2:608973/143‑1 (MQ=255)
                                                                                                                                                   gCGTAATCGCCGGCGATTTTGATCGTCACGGATTTAATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTTCCGt  <  1:1058866/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTTCCGT  >  CP009273/3028931‑3029226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑