Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,385,528 T→C 5.0% intergenic (‑141/+289) tldD ← / ← yhdP putative peptidase/DUF3971‑AsmA2 domains protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,385,5280TC 5.0% 307.5 / 2.3 130intergenic (‑141/+289)tldD/yhdPputative peptidase/DUF3971‑AsmA2 domains protein
Reads supporting (aligned to +/- strand):  ref base T (62/58);  new base C (4/5);  total (66/64)
Fisher's exact test for biased strand distribution p-value = 7.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 131 total aligned reads displayed.

TCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGA  >  CP009273/3385384‑3385676
                                                                                                                                                |                                                                                                                                                     
tCATCGGTGTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGa                                                                                                                                                   >  1:590234/1‑149 (MQ=255)
  aTCTTTTTTGCTACCCGTTGGTTACTGCAGTAGAGGACTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATCTTCCGGTTTTGAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATg                                                                                                                                                 <  1:578391/149‑1 (MQ=21)
  aTCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATg                                                                                                                                                 <  1:151437/149‑1 (MQ=255)
      tttttGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGGTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCAACAGGCAACGGCTGTCGGa                                                                                                                                                   <  2:727760/143‑1 (MQ=255)
       ttttGCGACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCACCAGGCAACGGCTGTCGGATgcggcg                                                                                                                                            <  1:729883/149‑1 (MQ=255)
        tttGCTACTCCTTAGCTCCCGCAGTAGAGGATTTTTTACGGCCGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGt                                                                                                                                           <  1:937914/149‑1 (MQ=21)
            cTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATgcggc                                                                                                                                             <  2:877950/143‑1 (MQ=255)
            cTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATgcggc                                                                                                                                             >  2:126246/1‑143 (MQ=255)
            cTACTCGTTAGTTACTGCAGTAGAGAATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAAc                                                                                                                                       >  1:654153/1‑149 (MQ=255)
             tACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACg                                                                                                                                      >  1:1298857/1‑149 (MQ=255)
              aCTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGc                                                                                                                                     >  1:767031/1‑149 (MQ=255)
                   ttagttaCTGCAGTCGAGGATTTGTTACGGCTGCCCGATGCGGCGTGAACCCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTAt                                                                                                                                <  1:445703/149‑1 (MQ=18)
                   ttagttaCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTAt                                                                                                                                <  1:1108153/149‑1 (MQ=255)
                   ttagttaCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAACGAGCAGTTATGTTCCGGTTTGTAGGCCTGCTAAGACGCACCGCGTCGCCTCAGGCACCGGCTTCCTGATGCGGCGTAAACTCCTTAt                                                                                                                                >  1:39330/1‑149 (MQ=37)
                         aCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGccc                                                                                                                          >  1:105812/1‑149 (MQ=255)
                           tGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTCCGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGGCCTa                                                                                                                        >  1:185974/1‑149 (MQ=255)
                           tGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATcc                                                                                                                              >  2:627184/1‑143 (MQ=255)
                           tGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATcc                                                                                                                              >  2:598560/1‑143 (MQ=255)
                           tGCAGTAGAGGATTTTTGACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATcc                                                                                                                              <  2:594262/143‑1 (MQ=255)
                            gCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTAc                                                                                                                       <  1:812332/149‑1 (MQ=255)
                             cAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTGTGTAGGCCTGATAAGACGCACAGCGTCGCATCTGGCAACGGCGGTCGGATGCGGCGTACACGCCTTAACCgc                                                                                                                            >  2:637117/1‑142 (MQ=18)
                              aGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGAc                                                                                                                           <  2:1101090/143‑1 (MQ=255)
                                tAGAGGATTTTTTCGGGCTGCCTGATGCGGCGTGACCGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGtt                                                                                                                   <  1:508589/149‑1 (MQ=255)
                                 agagGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTa                                                                                                                  >  1:642013/1‑149 (MQ=255)
                                     gATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCTGGTTTGTGGGCCTGTTAAGACGCAGAGCGTCGCATCAGGCGGCGGCTGTGGGATGGGGCGTAAACGCTTTGTcgggcgtccggt                                                                                                                    >  2:209462/1‑132 (MQ=17)
                                     gATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGtt                                                                                                              >  1:693383/1‑149 (MQ=255)
                                       ttttttACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTcc                                                                                                            <  1:809082/149‑1 (MQ=255)
                                        tttttACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCg                                                                                                           <  1:1200237/149‑1 (MQ=255)
                                            tACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGATGCGTAAACTCCTTATCCGACCTACGGTTATGTTc                                                                                                             <  2:1107894/143‑1 (MQ=255)
                                             aCGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTcc                                                                                                            <  2:150428/143‑1 (MQ=255)
                                             aCGGCTGCCGGAGGCGGCGTGAACGCCCTATCCAGCCTCCGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTcc                                                                                                            <  2:258995/143‑1 (MQ=255)
                                                gCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGCTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAgg                                                                                                   >  1:895115/1‑149 (MQ=255)
                                                 ctgctgGATGCGGCGGGAACGCCCTATCCGGCCTACGGTTATGTTTCGGTTTGTAGGCCGGCTAAGACGCCCAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGttt                                                                                                        <  2:1416/143‑1 (MQ=14)
                                                 cTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGGAGGc                                                                                                  >  1:1282790/1‑149 (MQ=255)
                                                 cTGCCGGATGCGGCCGGAACGCCCTACCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGc                                                                                                  <  1:1019482/149‑1 (MQ=255)
                                                  tGCCGGTTGCGGCGTGAACGCCCTATCCCGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGct                                                                                                 <  1:120175/149‑2 (MQ=255)
                                                    ccGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGCCCTACGGTGATGTTCCGTTTGTTGGCCTg                                                                                               >  1:466349/1‑149 (MQ=255)
                                                      ggATGCGGCGTGAACCCCCAATCCAGCCTACGGTGATGGTCCCGATCGCCCCGCTTATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGAt                                                                                             <  1:942459/149‑1 (MQ=14)
                                                      ggATGCGGCGGGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGAt                                                                                             <  1:1093033/149‑1 (MQ=255)
                                                      ggAGGCGGCGTGGACGCGCTATCCAGCCTGCGGTTAGGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCTTTTGGCAACGGCTGTCGGAGGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGAt                                                                                             <  1:503772/149‑1 (MQ=14)
                                                       gATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCGGTTTGTGGGc                                                                                                  >  2:395127/1‑143 (MQ=255)
                                                       gATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTACCCGACCTCCGGTTATGTTCCGTCTGTAGGc                                                                                                  >  2:513893/1‑143 (MQ=255)
                                                           cgtggTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGa                                                                                              <  2:591327/139‑1 (MQ=255)
                                                           cggcgTGACACCCCTATCCAGCCTACGGTTGCGTGCCGGTTTGTCGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGTATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGa                                                                                              <  2:892819/143‑1 (MQ=14)
                                                           aggcgTGAACGCCCTCTCCAGCCTGCGGTTTTGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCAGCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGa                                                                                              <  2:31127/142‑1 (MQ=255)
                                                            ggcgTGAACGCCCTGTCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCCCAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGGTCTGTTCCGTTTGTGGGcccggt                                                                                             >  2:887725/1‑139 (MQ=17)
                                                            ggcgTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGAt                                                                                             <  2:167908/143‑1 (MQ=255)
                                                            ggcgTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGGTATGTTCCGGTTGTAGACCTGAt                                                                                             >  2:1024569/1‑143 (MQ=255)
                                                             gcgTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTGTGTTCCGTTTGTTGGCCTGATAAGACGc                                                                                      >  1:802624/1‑149 (MQ=255)
                                                             gcgTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATa                                                                                            >  2:410808/1‑143 (MQ=255)
                                                               gTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAATACGccc                                                                                    >  1:89592/1‑147 (MQ=255)
                                                                agtgttcccTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGa                                                                                         <  2:505980/137‑1 (MQ=255)
                                                                  aaCGCCCTATCCAGCCTGCGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTACCCGACCTACGGTTATGTTCCGATTGTAGGCCTGATAAGACGCACAGc                                                                                 <  1:730700/149‑1 (MQ=255)
                                                                   aCGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACGTAGGGGTATGTTCCGTTTGTAGGCGTGGTAAGACACACAGCg                                                                                >  1:638441/1‑149 (MQ=14)
                                                                   aCGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACCGCTGTCGGGTGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCGGTTTGTAGGCCTGGTAAGACGCACAGCg                                                                                >  1:377259/1‑149 (MQ=255)
                                                                    cGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGcc                                                                                     >  2:83269/1‑142 (MQ=255)
                                                                      cccTTTCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGcaca                                                                                   <  2:412539/143‑1 (MQ=255)
                                                                         tATCCAGCCTGCGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCg                                                                                <  2:388767/143‑1 (MQ=255)
                                                                          aTCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTACACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTACGCCTGCTatcaacgcccgtct                                                                               >  2:218606/1‑130 (MQ=14)
                                                                          aTCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGCTAAGACGCACAGccccaagat                                                                         >  1:613948/1‑141 (MQ=255)
                                                                           tCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGCTTGTTGGCCTGATAAGACGCAAAGCCTc                                                                              >  2:888345/1‑143 (MQ=11)
                                                                            gcAGCCTACGGTGATGTTCCGGTTTGTAGGCCTGAGAAGACCCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCg                                                                             <  2:455677/142‑1 (MQ=255)
                                                                                cccaccgtttatttCCGGTTTTCAGGCCTGATAAGACGCCCAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCCACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATc                                                                         <  2:927441/131‑1 (MQ=11)
                                                                                ccTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTATGCCTGATCAGACGCACAGCGTCgtcac                                                                         >  2:357436/1‑139 (MQ=255)
                                                                                   aCGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCCTATCCGACCTACGGTTATGTTCCGTTTGTTGGCCTGATAACACGCACAGCGTCGCAGCAgg                                                                      >  2:645995/1‑143 (MQ=255)
                                                                                            ttCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCCACGGCTGCCGGAt                                                      >  1:704801/1‑149 (MQ=37)
                                                                                            ttCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGGTTGTAGGCCTGATAAGACCCACAGCGCCGCATCAGGCAACGGCTg                                                            >  2:564794/1‑143 (MQ=255)
                                                                                                 gTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGa                                                       <  2:830962/143‑1 (MQ=32)
                                                                                                     gtgggCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcg                                                   <  2:392988/140‑1 (MQ=17)
                                                                                                        ggCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACg                                          >  1:1036448/1‑149 (MQ=35)
                                                                                                         gCCTGATAGGACGCAGAGCGCCGCATCAGGCCACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTCGGGTTATTTCCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGt                                               <  2:679794/143‑1 (MQ=11)
                                                                                                         gCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTTGGCCCTATAACAACCACAACACCGCATCAGGCAGCGGCTGCCGGGCgcggccc                                               >  2:232265/1‑141 (MQ=11)
                                                                                                         gCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCGCAGCGTCGCATTAGGCGACGGGTGCCGGGTGCGGCGt                                               >  2:574158/1‑143 (MQ=11)
                                                                                                         gCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGc                                         <  1:146095/149‑1 (MQ=35)
                                                                                                         gCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCCTCGCATCAGGCAACGGCTACCTCATgcggcgg                                               >  2:1035976/1‑142 (MQ=255)
                                                                                                            tGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGAGGCGGCGTGAACGCCtt                                      >  1:1128999/1‑149 (MQ=14)
                                                                                                            tGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAAAGGCTGCCGGATGCGGCGGAAACCCCtt                                      >  1:608283/1‑149 (MQ=255)
                                                                                                            tGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGCTGCGTTGGTAGGCGTGAGAAGAGGCAGATCGACGCATCGGGCAACGGCTGCGGGGCGCGGGGTaaa                                            >  2:229923/1‑143 (MQ=11)
                                                                                                              acaaGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTAt                                    <  1:1253975/147‑1 (MQ=38)
                                                                                                               tAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATc                                   >  1:1248570/1‑149 (MQ=37)
                                                                                                                   aagCACAGCGTCGCATCAGGCAACGGCTGTCGGAGGGGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGAc                               <  1:291572/147‑1 (MQ=255)
                                                                                                                     gCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATc                                   >  2:1059233/1‑143 (MQ=37)
                                                                                                                       acaGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACCCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTAc                           >  1:676397/1‑149 (MQ=38)
                                                                                                                        caGCGTCGCATCAGGCCACGGCTGTCGGATGCGCCTTAACCGCCTTATCCGCCCTACGGTTATGTTCCGGTTGTAGGCCGTATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGa                                <  2:65798/143‑1 (MQ=11)
                                                                                                                         aGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACgg                         <  1:96844/149‑1 (MQ=38)
                                                                                                                         aGCGTCGCATCAGGCAACGCCGGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACgg                         <  1:645803/149‑1 (MQ=255)
                                                                                                                           cGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGtt                       >  1:1055024/1‑149 (MQ=38)
                                                                                                                            tgcGCATCCGGCAACGGCGGTCGGTGGAGGGTTAATCTGCTAACCCGACTGACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTa                            <  2:797837/141‑1 (MQ=11)
                                                                                                                            gTCCCATCAGGCACCGGCTGTCGGGTGCGGCGTAACCGCCTTATCCGCCCTCCGCTTATTTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTa                            <  2:680510/143‑1 (MQ=14)
                                                                                                                                cctCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGtt                  <  1:383090/147‑1 (MQ=38)
                                                                                                                                cATCAGGCAAGGCGTGTCTGATGCGGCTTAAACGCCTTATCCGACCTACGGTTATGTTCGGTTTGTAGGCCTTCTACGACACACAGCGTCGCACACGGATTGTACTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGt                        <  2:1103026/143‑1 (MQ=2)
                                                                                                                                 atcttgCAACGGCGGCCGGATGCGGCGTAAACGCCTAATCCGACCTACGGTTAGGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGtt                       <  2:91524/138‑1 (MQ=11)
                                                                                                                                  tCAGGCAACGGCTGCGGGATGCGGCGTAAACGCCTTATCCGCCCTAGGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTa                      <  2:549157/143‑1 (MQ=255)
                                                                                                                                      gCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTAAGTTCCGttt            >  1:166929/1‑149 (MQ=255)
                                                                                                                                      gCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCCTATCCGACCTACGGTTCTGtt                  >  2:1089268/1‑143 (MQ=17)
                                                                                                                                        aaCGGCTGTCGGATGCGGCGTAAACGCCTTTTCCGTCCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGt          <  1:240426/149‑1 (MQ=255)
                                                                                                                                            gCTGTGGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGGCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGc      <  1:583315/149‑1 (MQ=255)
                                                                                                                                            gCTGTCGGATGCGGCGTAACCGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGc      <  1:1223139/149‑1 (MQ=255)
                                                                                                                                            gCTGGCGGATGCGGCGTAAACGCCTTATCCGACCTAGGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGc      <  1:477672/149‑1 (MQ=255)
                                                                                                                                                tCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGa  >  1:923759/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
TCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGA  >  CP009273/3385384‑3385676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: