Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 416,402 T→A 7.8% intergenic (+36/‑40) brnQ → / → proY branched‑chain amino acid transport system 2 carrier protein; LIV‑II transport system for Ile, Leu, and Val/proline‑specific permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273416,4020TA 7.8% 208.0 / 8.8 79intergenic (+36/‑40)brnQ/proYbranched‑chain amino acid transport system 2 carrier protein; LIV‑II transport system for Ile, Leu, and Val/proline‑specific permease
Reads supporting (aligned to +/- strand):  ref base T (37/34);  new base A (2/4);  total (40/39)
Fisher's exact test for biased strand distribution p-value = 4.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.02e-01

CTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGA  >  CP009273/416259‑416545
                                                                                                                                               |                                                                                                                                               
cTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGcccc                                                                                                                                            >  1:891749/1‑149 (MQ=255)
cTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGcccc                                                                                                                                            <  1:477254/149‑1 (MQ=255)
 tgcccGAACAGGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCg                                                                                                                                           <  1:34958/146‑1 (MQ=255)
    ccGAACAAGGTCTGGCGTGGTTAGTGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTgg                                                                                                                                        <  1:905065/149‑1 (MQ=255)
       aaCAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGATTCTGGCCATTATCTGGGATCGCACGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCGCGTGGttt                                                                                                                                     >  1:1115390/1‑149 (MQ=255)
         cAAGGGCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTg                                                                                                                                         <  2:388427/143‑1 (MQ=255)
          aaGGTCTGGCGTTGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTATCCACGGGGCTGCGATGCCCCGTgg                                                                                                                                        <  2:897410/143‑1 (MQ=255)
            ggTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTAt                                                                                                                                >  1:52218/1‑149 (MQ=255)
             ctCTTGCGTGGTTAATGCCAACAGTGGTGATGGTGCTTCTGGCCATTATCTGGGATCGCGCGGCCGGTCGTCAGGTGCCCTCCAGCGCGCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTAtt                                                                                                                               <  1:22312/148‑1 (MQ=255)
               cTGGCGGGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGGCCCGTGGTTTTTTATtgt                                                                                                                             <  1:957592/149‑1 (MQ=255)
                   cgcggTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGGCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTAtt                                                                                                                               <  2:243450/140‑1 (MQ=255)
                   cGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTAtt                                                                                                                               <  2:326930/143‑1 (MQ=255)
                   cGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTg                                                                                                                         >  1:414603/1‑149 (MQ=255)
                     ttgTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCATCGCAGCCCCGTGGttaaaacaaat                                                                                                                             <  2:1116451/141‑10 (MQ=38)
                       gTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATgg                                                                                                                     <  1:420209/149‑1 (MQ=255)
                        ttAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATggg                                                                                                                    >  1:1261824/1‑149 (MQ=255)
                          aaTGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTGTTTTATTGTGTTGa                                                                                                                        >  2:914991/1‑143 (MQ=255)
                          aaTGCCAACAGTGGGGATGGTGGTTCTGGCCATTAGCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATGGTGTTGa                                                                                                                        >  2:1140019/1‑143 (MQ=255)
                              ccactcgaggagATGGGGGTTCTGTCCATTATCTGGGAGCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGCTTTAACCACGAGGCTGCGATGTCCCGGGGTTTTTTATTGTGTTGATGGGTTAGGa                                                                                                              <  1:120487/138‑1 (MQ=255)
                               cAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGt                                                                                                                   >  2:969139/1‑143 (MQ=255)
                                aaCAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGATCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTACTGTGTTGATGGGtt                                                                                                                  >  2:839879/1‑143 (MQ=255)
                                   aGTGGTGATGGTGGTTCTGGCCATTATCTGGGATGGCGCGGCAGGTGGTGAGGTGACCTCCAGCGCTCACTAAATCACTGAACGTTTGTTTTAACCACGGGGGTGGGGTGCCCCGTGGTTGTTTGTTGTGTTGTTGGGTTAgg                                                                                                               >  2:339691/1‑143 (MQ=255)
                                     tggtgATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCGCTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTGTTTATTGTGTTGATGGGTTAGGAATTGATg                                                                                                       >  1:361589/1‑149 (MQ=255)
                                     tggtgATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGCGGTTTTTTATTGTGTTGATGGGTCAGGaa                                                                                                             >  2:1064183/1‑143 (MQ=255)
                                      ggtgATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGCACCTTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGaag                                                                                                            >  2:306371/1‑142 (MQ=255)
                                         gATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATGTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTTATGTGTTGATGGGTTAGGAATTGATGGaaa                                                                                                   <  1:797324/149‑1 (MQ=255)
                                         gATGGTGGTTCGGGCCAGTGTCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGATGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGaaa                                                                                                   <  1:420359/149‑1 (MQ=255)
                                           tggtggTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATg                                                                                                       <  2:309252/143‑1 (MQ=255)
                                             gtggtTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTaa                                                                                               >  1:770719/1‑149 (MQ=255)
                                               ggtTCTGGCCATTATCTGGGAACGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCACACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTAATTGTGTTGCTGGGTTAGGAATTGATGGaaa                                                                                                   >  2:388068/1‑143 (MQ=255)
                                                   cTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTaa                                                                                               >  2:585678/1‑143 (MQ=255)
                                                       acATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGGGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTa                                                                                     <  1:352835/148‑1 (MQ=255)
                                                        cATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTaa                                                                                    >  1:390503/1‑149 (MQ=255)
                                                          cgggtacttagcccacgctttagcttgttcttactttccatcaattcctaacccatcaacacaataaaaAACCACGGGGCATCGCAGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAg                                                                                  <  1:488839‑M1/80‑1 (MQ=255)
                                                               tGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTaaa                                                                                   <  2:1048790/143‑1 (MQ=255)
                                                                gggATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTggg                                                                            >  1:197232/1‑149 (MQ=255)
                                                                  gATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCg                                                                                >  2:640828/1‑143 (MQ=255)
                                                                  gATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCg                                                                                >  2:561429/1‑143 (MQ=255)
                                                                       cgcgGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTa                                                                     >  1:1088854/1‑149 (MQ=255)
                                                                           gCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAg                                                                       <  2:527164/143‑1 (MQ=255)
                                                                              ggTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGAGGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCca                                                              <  1:253681/149‑1 (MQ=255)
                                                                               gtcgtcAGGTGACCTCCAGCGCTCACTAAATCAGTGAACATTTGTTTTACCCACGGGGCTGCGATGCCCCGCGGTTTTTTATTGGGTTGATGGGTTAGGAATTGCTGGAACGTCAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCcac                                                             >  1:836886/1‑149 (MQ=255)
                                                                                   tcAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATAc                                                         >  1:574429/1‑149 (MQ=255)
                                                                                       gTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCtt                                                     >  1:66915/1‑149 (MQ=255)
                                                                                        tGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCttt                                                    <  1:811418/149‑1 (MQ=255)
                                                                                         tactttccatcaattcctaacccatcaacacaataaaaAACCACGGGGCATCGCAGCCCCGAGGATTTTTATTGTGTTGATGGGTTAGGAATTCATGGAAAGTAAGAACAAGCTAAAGCTGGGGCTAAGTACCCGCCACATAc                                                         <  2:98786‑M1/105‑1 (MQ=255)
                                                                                         gACCTCCAGCGCTCACTAAATCCCTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTa                                                   <  1:64836/149‑1 (MQ=255)
                                                                                           ccTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAGCAGCGGGGCTGCGAGGCCCCGTGGATTTTTATGGTCTTGATGGGTTAGGGATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCGCCCACATCCGc                                                       >  2:2618/1‑143 (MQ=255)
                                                                                           ccTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTAGTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGc                                                       <  2:215880/143‑1 (MQ=255)
                                                                                              gcAGGGCTCACTAAGGCGGGGAACATTTGTGTGAGCGACGGGGCGGCGGTGGCGCGTGGTTTTTTATTGTGTTGATGGGGTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCa                                              <  1:1179462/148‑1 (MQ=255)
                                                                                               cAGCGCTTACTACATCACCGACCATTTGTTTTAACCACGTGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTa                                                   <  2:587618/143‑1 (MQ=255)
                                                                                               cAGCGCTCACTAAATCCCTGAACATTTGTTTTAACCACGGGGTTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTTCCCGCCACATACGCTTTa                                                   <  2:282063/143‑1 (MQ=255)
                                                                                                    cTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGt                                        >  1:1087862/1‑149 (MQ=255)
                                                                                                    cTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGAGGCCCCGTGGTTTATTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTCCCCGCCACATACGCTTTATGGCa                                              <  2:776079/143‑1 (MQ=255)
                                                                                                         tAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGc                                   >  1:177381/1‑149 (MQ=255)
                                                                                                         tAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGAGTTCAGc                                   >  1:230780/1‑149 (MQ=255)
                                                                                                           aacccatcaacacaataaaaAACCACGGGGCATCGCAGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGtt                                       >  2:420142‑M1/21‑143 (MQ=255)
                                                                                                            aTCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCaag                                >  1:1119504/1‑148 (MQ=255)
                                                                                                             tCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGTGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAAtt                               <  1:894265/149‑1 (MQ=255)
                                                                                                             tCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAAtt                               <  1:993770/149‑1 (MQ=255)
                                                                                                             tCACGGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAAtt                               <  1:985555/149‑1 (MQ=255)
                                                                                                              cACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAg                                    <  2:608252/143‑1 (MQ=255)
                                                                                                                     cATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTgg                             <  2:946293/143‑1 (MQ=255)
                                                                                                                     cATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTgg                             <  2:438024/143‑1 (MQ=255)
                                                                                                                         tGTTTTAACCACTGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGTTGGGTTAGGAATTGGTGGAAAGTAAGTACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCAcc                         <  2:404134/143‑1 (MQ=255)
                                                                                                                           ttttAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGtt                 >  1:53497/1‑149 (MQ=255)
                                                                                                                           ttttAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGAAATTGGCACCGGGcgggt                 >  1:547452/1‑145 (MQ=255)
                                                                                                                             ttAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACGCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGc                     >  2:126608/1‑143 (MQ=255)
                                                                                                                             aaAACCACGGGGCATCGCAGCACCGTGGTTTTTTATTGTGTTGATGGGATAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCtttttt               <  1:197460‑M1/147‑1 (MQ=255)
                                                                                                                              tacCCACGGGGTTGCGATGCCCCGTGGTTTTTTATTGTGTTTATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTa              >  1:509285‑M1/4‑149 (MQ=255)
                                                                                                                              gAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAATTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTa              >  1:1154845‑M1/2‑149 (MQ=255)
                                                                                                                              aAACCACGGGGCATCGCAGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTa              >  1:114519‑M1/2‑149 (MQ=255)
                                                                                                                               AACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCGAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTg                   >  2:723786‑M1/1‑143 (MQ=255)
                                                                                                                                  CACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGAGGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGttt                >  2:1116692‑M1/1‑143 (MQ=255)
                                                                                                                                     GGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAAGTGGCCCCGGGCTGTTTTACGGTTCg       <  1:8305‑M1/149‑1 (MQ=255)
                                                                                                                                      GGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGTAATGGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCCAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACg            >  2:555579‑M1/1‑143 (MQ=255)
                                                                                                                                          tGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGa  <  1:1071584/149‑1 (MQ=255)
                                                                                                                                          tGCGATGCCCCGTGGTTTTTTATTGCGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTc        <  2:337216/143‑1 (MQ=255)
                                                                                                                                            cGATGCCCCGTGGTTATTTATTGTGTTGATTGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTGTCGGTTCga      >  2:985224/1‑142 (MQ=255)
                                                                                                                                               |                                                                                                                                               
CTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGA  >  CP009273/416259‑416545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: