Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,029,079 C→T A188T (GCT→ACT)  prfB ← peptide chain release factor RF‑2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,029,0790CT95.0% 173.5 / NA 62A188T (GCT→ACT) prfBpeptide chain release factor RF‑2
Reads supporting (aligned to +/- strand):  ref base C (2/0);  major base T (26/31);  minor base G (3/0);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 1.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCT  >  CP009273/3028939‑3029216
                                                                                                                                            |                                                                                                                                         
gATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAgtgt                                                                                                                                         <  2:91255/143‑1 (MQ=255)
  tttCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTaa                                                                                                                                       <  2:531809/143‑1 (MQ=255)
  tttCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGGAAGGGTaa                                                                                                                                       >  2:771283/1‑143 (MQ=255)
   tccGATTTCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCgg                                                                                                                                <  1:180634/147‑1 (MQ=255)
         atcaatatcaTCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGGGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGAttt                                                                                                                          <  1:15329/149‑1 (MQ=255)
         accaatttcaTCCTCAACTTCCGGATAAACAACCGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGACTCAAACGGGCTTTTACGCTCCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGAttt                                                                                                                          <  1:692654/147‑1 (MQ=255)
               atcatcatcAACTTCCGGATAAACAAACGCGGAGCTGAACGTCGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGAttt                                                                                                                          <  2:313140/143‑1 (MQ=255)
                      tcaACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGt                                                                                                                   <  2:404791/143‑1 (MQ=255)
                      tcaACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCGCGGa                                                                                                             >  1:889453/1‑149 (MQ=255)
                      tcaACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGa                                                                                                             >  1:678109/1‑149 (MQ=255)
                       caACTTCCGGATAAACAAACGCGCAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGGGATTTTGATCGtt                                                                                                                  >  2:566198/1‑142 (MQ=255)
                          cTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTa                                                                                                         <  1:188086/149‑1 (MQ=255)
                             ccGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCCCGGaa                                                                                                            >  2:674373/1‑142 (MQ=255)
                             ccGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATa                                                                                                      <  1:820528/149‑1 (MQ=255)
                             ccGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTGTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTTATCGCCGGAGATTTTGATCGTCACGGAt                                                                                                            >  2:224530/1‑143 (MQ=255)
                                       aaacGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCACAAGTGGAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCg                                                                                                  >  2:454969/1‑143 (MQ=255)
                                        aacGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTc                                                                                           >  1:426973/1‑149 (MQ=255)
                                          cgcgGAGCCGAACGACGTGGGGCGAGGGCCGCCGGAGTCAAATGGGCTTTTGCGCACCAGGCGGTGAACGCCGGTTTTTGTACGCCGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCa                                                                                               <  2:288143/143‑1 (MQ=255)
                                           gcgGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGGGAACGCCGGTTTCTGTACGCAGGCAGCCGTCAGGGTCATAGCCGGAGATTTTGTGCGTCACGGATTTAAtcacccccgc                                                                                              >  2:263249/1‑134 (MQ=255)
                                                   gAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGAct                                                                                <  1:841485/149‑1 (MQ=255)
                                                   gAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAAGCGACGGAGATTTTGCTAGTCCCGGTGTTAATAACCGCCAcgtaccctt                                                                                      >  2:742289/1‑135 (MQ=255)
                                                    aaCGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCGCCTTCACCTTc                                                                                     >  2:373507/1‑143 (MQ=255)
                                                       gacgTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGGCGGTGTCGGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGAGTTAATACCCGCCACTTCCCCTTCCGCCTCTTc                                                                            >  1:131433/1‑149 (MQ=255)
                                                          gtgtGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGGTTTAATTCCCGCCACTTCACCTTTCGACTCTTCgat                                                                         >  1:207493/1‑149 (MQ=255)
                                                              ggCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCg                                                                           <  2:238030/143‑1 (MQ=255)
                                                               tcgtcgcacggcgGATTTAAACGGGCTTTTTCTCACCATGCTGGGAGCGCCTTTTTCTTGTGGCAGCCATCCGTAAGTGGATTTTCCGGATATTTTGCTCGGCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCga                                                                          <  2:23572/132‑1 (MQ=255)
                                                               gCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCTGCCAGCCGTAAGTGTAATCGCCGGAGGTTTTGATCATCACGGATTTTATACCCGCCCCTTCACCTTCCGACTCTTCGTTGAttc                                                                    >  1:149741/1‑148 (MQ=255)
                                                               gCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCga                                                                          >  2:619921/1‑143 (MQ=255)
                                                                cgacgaCCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTACGGGTAATCGCCGGAGATTTTGATCCTCACGGACTTAAAACCCGCCACTTCCCCTTcccaaccctcgaa                                                                         >  2:338857/1‑132 (MQ=255)
                                                                cgacgaCCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGGGAACGCCGGTTTCTGTACGCCGCCAGCCGTAAGTGTAATCGCCGGAGACTTTGATCGTGACGGACTTAATACCCGCCCCTTCACCTTCCGACTCttggct                                                                         >  2:305250/1‑139 (MQ=255)
                                                                cgacgaCCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGCGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCgat                                                                         <  2:252705/143‑1 (MQ=255)
                                                                 ggcggccgccgGGGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGGACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCa                                                                  <  1:130056/144‑1 (MQ=255)
                                                                 gacgacCCCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATCTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCa                                                                  <  1:261609/149‑1 (MQ=255)
                                                                     acCGCCGGAGTCAACCGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGAttt                                                                    <  2:786449/143‑1 (MQ=255)
                                                                     acCGCCGGAGTCAAACGGGCTTTTACGCACCATGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGttt                                                              <  1:387109/149‑1 (MQ=255)
                                                                     acCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGAttt                                                                    <  2:786495/143‑1 (MQ=255)
                                                                     acCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGttt                                                              >  1:749381/1‑149 (MQ=255)
                                                                     acCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATAGTCACGGCTTTAATAACCGACCATTCACCTTACGCTTCTTCgagagtgt                                                                    >  2:365091/1‑137 (MQ=255)
                                                                      ccgccgGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGtttt                                                             <  1:68601/149‑1 (MQ=255)
                                                                       ggccgGAGCCAAACGGGCTTGTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCCGCCACCCTTAAGTTTACTCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCa                                                                  <  2:4274/142‑1 (MQ=255)
                                                                       cgccgGTGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCa                                                                  <  2:600386/143‑1 (MQ=255)
                                                                       cgccgGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTg                                                            >  1:735649/1‑149 (MQ=255)
                                                                       cgccgGAGACAACCGGGCTTTTACGCACCAGGCGGTGAACGCGGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCAAGGATTTAATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCa                                                                  <  2:400806/143‑1 (MQ=255)
                                                                         ccgGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGt                                                                >  2:489789/1‑143 (MQ=255)
                                                                            gAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGtttt                                                             <  2:242932/143‑1 (MQ=255)
                                                                                 aaaCGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCTCTTCACCTTCCGACTCTTCGATGATATCAGTTTTGAAAc                                                        <  2:504796/143‑1 (MQ=255)
                                                                                 aaaCGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAAc                                                        <  2:312555/143‑1 (MQ=255)
                                                                                 aaaCGGCCTTTTACGCACCAGGCGTTGACCGCGTGTTCCTGCACGCAGCCACCCGTAAGTCTAATCGCCGGAGATTTTGATCGTCACGGATTTAACACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAAc                                                        <  2:427793/143‑1 (MQ=255)
                                                                                        cTTTTACGCACCAGTCGTGGGACGCCGTTTTCTCTACTCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGACCGTCACGTATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGa                                                 <  2:26011/143‑1 (MQ=255)
                                                                                         ttttACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTACTACCCGCCACTTCACCTTCCGACTCTCCGATGATTTCAGTTTTGAGACCACGCGATTCGGcc                                          >  1:29008/1‑149 (MQ=255)
                                                                                         ttttACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGcc                                          >  1:310163/1‑149 (MQ=255)
                                                                                         ttttACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCACCAAGCCGTAACTGAAAACACCATAGTGCGAAATCGGCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGAt                                                >  2:805523/1‑143 (MQ=255)
                                                                                            tACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAg                                       >  1:853447/1‑149 (MQ=255)
                                                                                                   cAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCCGCCCAGCGCAGat                                <  1:625282/149‑1 (MQ=255)
                                                                                                    aGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGata                               <  1:766902/149‑1 (MQ=255)
                                                                                                      gCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGCTTTAATACACGACACTTCACATTACGAATCTTAGATGCTTTCAGTTTTGAAACCACGCGCTTCTGCCCAgcgcc                                   >  2:18050/1‑142 (MQ=255)
                                                                                                             aCGCCGTTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCCTCTAGGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTc                      <  1:180807/149‑1 (MQ=255)
                                                                                                                   gTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCCGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCAt                >  1:313122/1‑149 (MQ=255)
                                                                                                                     ttCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGc              >  1:472179/1‑149 (MQ=255)
                                                                                                                     ttCTGTACGCAGCCAGCCGTAAGTGTAATCCCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCaa                    <  2:80235/143‑1 (MQ=255)
                                                                                                                        tGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCa                 >  2:162847/1‑143 (MQ=255)
                                                                                                                                     ccGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAgcc    >  2:170797/1‑141 (MQ=255)
                                                                                                                                       gTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCCCGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCt  >  2:23895/1‑143 (MQ=255)
                                                                                                                                            |                                                                                                                                         
GATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCT  >  CP009273/3028939‑3029216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑