Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,297,1070TC29.0% 32.0 / 15.0 32G170G (GGA→GGG) alsKD‑allose kinase
Reads supporting (aligned to +/- strand):  ref base T (13/9);  new base C (2/7);  total (15/17)
Fisher's exact test for biased strand distribution p-value = 1.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.96e-02
Rejected as consensus: Frequency below/above cutoff threshold.

GGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTC  >  CP009273/4296968‑4297250
                                                                                                                                           |                                                                                                                                               
gggCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACCCGCGCAGGGTTGGGTCATATCTCCCa                                                                                                                                             >  1:429841/1‑144 (MQ=255)
      tttCCGCATGGACGAAAAGATCGCCCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGa                                                                                                                                  <  2:110099/149‑1 (MQ=255)
      gatctgCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGa                                                                                                                                  <  2:176128/144‑1 (MQ=255)
       ttCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGAc                                                                                                                                 <  2:570548/149‑1 (MQ=255)
          cGCATGGACGAAAAGATCGGGCAAGGGGTAATTTCGGAGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCa                                                                                                                              <  2:231295/149‑1 (MQ=255)
            cATGGACGAAACGATCGCGCAATGGCTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGAc                                                                                                                                 <  1:353237/144‑1 (MQ=255)
            cATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCAATTCCAGAGCACTTGGTTTCCAGGCACCCAGGAATGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGAc                                                                                                                                 >  1:160627/1‑144 (MQ=255)
             tgggACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCGGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGtt                                                                                                                           <  2:496174/147‑1 (MQ=255)
              tGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGAccc                                                                                                                               <  1:11943/144‑1 (MQ=255)
               ggccGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCg                                                                                                                         <  2:256157/146‑1 (MQ=255)
                   gAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTg                                                                                                                     >  2:328837/1‑149 (MQ=255)
                                      tAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACTCCGTGTGCAccaca                                                                                                  >  2:720698/1‑146 (MQ=255)
                                           tCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCAcc                                                                                             >  2:346650/1‑148 (MQ=255)
                                               ggCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCAc                                                                                              >  1:59984/1‑144 (MQ=255)
                                                 cTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACgg                                                                                            >  1:492960/1‑144 (MQ=255)
                                                   gttgttCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGCTATGACCCAGTTCGCCTGCCCCACCGTGTGCACCCGTCCgcggcg                                                                                          >  1:412111/1‑144 (MQ=255)
                                                              cAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGCCATATCTCCCAGGGGCATATGACCCAGTTCGCCTACCACACCGTGTGCACCCGTCCACGGCGCACCGTTCGTc                                                                               >  1:34393/1‑144 (MQ=255)
                                                                   tCTTAGCCCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGACATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGa                                                                     <  2:50821/149‑1 (MQ=255)
                                                                     ttAGCGCCATTCCAGAGCAATTGGTTCCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAAc                                                                   <  2:14117/149‑1 (MQ=255)
                                                                          gcCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCAt                                                              <  2:564329/149‑1 (MQ=255)
                                                                                  agagCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTAc                                                      >  2:312200/1‑149 (MQ=255)
                                                                                   gagCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATAGCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTAcc                                                     <  2:338667/149‑1 (MQ=255)
                                                                                      cagttGGTTTCGCGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCCTGTCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTa                                                       <  1:183819/141‑1 (MQ=255)
                                                                                      cAATTGGTTTCCAGGCACCCAGGATGGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTa                                                       <  1:604152/144‑1 (MQ=255)
                                                                                         ttGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCAGCGTTCATCCACACTGCGAACCCCATCCCCGTACCg                                                    >  1:399181/1‑144 (MQ=255)
                                                                                             attCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGACATCTCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGgcc                                           >  2:659503/2‑149 (MQ=255)
                                                                                                  aGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATACCTCCCAGGGGGATGTGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACTGCGCACCGCTCCTCCACACGGCGGACCCCACCCCCGTACCCAGCGAGgcg                                           >  1:392647/1‑143 (MQ=255)
                                                                                                         cAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGGGATAGGCCGCCAGAACCAgt                               >  2:151919/1‑149 (MQ=255)
                                                                                                                             tgtTGGGGCATATCCCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTc           <  2:84858/149‑1 (MQ=255)
                                                                                                                                 gggTCATATCTCCCAGGGCGATATGACCCAGTTCGCCTGCCACACCGGGTGCACCCGTCCACGGCGCACCGGTCATCCACCCTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGtttt            <  1:401908/144‑1 (MQ=255)
                                                                                                                                    tCATATCGCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTcta         <  1:295516/144‑1 (MQ=255)
                                                                                                                                    gcctacctcCCAGGGGGATATGCCCCAGTTCGCCGGCCACACCGTGTGCCCCCGTCCACGGCGCACCGTTCAGCCACACTGCGAACCCCACCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTcta         <  1:11429/138‑1 (MQ=255)
                                                                                                                                      atatCCCCCTGGGAGATATGACCCAGTTCGCCTGCCACACCATGTGCACCCATCCCCGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTc  >  2:177819/1‑149 (MQ=255)
                                                                                                                                           |                                                                                                                                               
GGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTC  >  CP009273/4296968‑4297250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑