Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,297,1110AT21.2% 51.5 / 13.1 33L169Q (CTG→CAG) alsKD‑allose kinase
Reads supporting (aligned to +/- strand):  ref base A (12/14);  new base T (2/5);  total (14/19)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-02
Rejected as consensus: Frequency below/above cutoff threshold.

GGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAG  >  CP009273/4296968‑4297257
                                                                                                                                               |                                                                                                                                                  
gggCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACCCGCGCAGGGTTGGGTCATATCTCCCa                                                                                                                                                    >  1:429841/1‑144 (MQ=255)
      tttCCGCATGGACGAAAAGATCGCCCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGa                                                                                                                                         <  2:110099/149‑1 (MQ=255)
      gatctgCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGa                                                                                                                                         <  2:176128/144‑1 (MQ=255)
       ttCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGAc                                                                                                                                        <  2:570548/149‑1 (MQ=255)
          cGCATGGACGAAAAGATCGGGCAAGGGGTAATTTCGGAGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCa                                                                                                                                     <  2:231295/149‑1 (MQ=255)
            cATGGACGAAACGATCGCGCAATGGCTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGAc                                                                                                                                        <  1:353237/144‑1 (MQ=255)
            cATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCAATTCCAGAGCACTTGGTTTCCAGGCACCCAGGAATGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGAc                                                                                                                                        >  1:160627/1‑144 (MQ=255)
             tgggACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCGGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGtt                                                                                                                                  <  2:496174/147‑1 (MQ=255)
              tGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGAccc                                                                                                                                      <  1:11943/144‑1 (MQ=255)
               ggccGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCg                                                                                                                                <  2:256157/146‑1 (MQ=255)
                   gAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTg                                                                                                                            >  2:328837/1‑149 (MQ=255)
                                      tAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACTCCGTGTGCAccaca                                                                                                         >  2:720698/1‑146 (MQ=255)
                                           tCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCAcc                                                                                                    >  2:346650/1‑148 (MQ=255)
                                               ggCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCAc                                                                                                     >  1:59984/1‑144 (MQ=255)
                                                 cTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACgg                                                                                                   >  1:492960/1‑144 (MQ=255)
                                                   gttgttCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGCTATGACCCAGTTCGCCTGCCCCACCGTGTGCACCCGTCCgcggcg                                                                                                 >  1:412111/1‑144 (MQ=255)
                                                              cAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGCCATATCTCCCAGGGGCATATGACCCAGTTCGCCTACCACACCGTGTGCACCCGTCCACGGCGCACCGTTCGTc                                                                                      >  1:34393/1‑144 (MQ=255)
                                                                   tCTTAGCCCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGACATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGa                                                                            <  2:50821/149‑1 (MQ=255)
                                                                     ttAGCGCCATTCCAGAGCAATTGGTTCCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAAc                                                                          <  2:14117/149‑1 (MQ=255)
                                                                          gcCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCAt                                                                     <  2:564329/149‑1 (MQ=255)
                                                                                  agagCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTAc                                                             >  2:312200/1‑149 (MQ=255)
                                                                                   gagCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATAGCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTAcc                                                            <  2:338667/149‑1 (MQ=255)
                                                                                      cagttGGTTTCGCGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCCTGTCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTa                                                              <  1:183819/141‑1 (MQ=255)
                                                                                      cAATTGGTTTCCAGGCACCCAGGATGGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTa                                                              <  1:604152/144‑1 (MQ=255)
                                                                                         ttGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCAGCGTTCATCCACACTGCGAACCCCATCCCCGTACCg                                                           >  1:399181/1‑144 (MQ=255)
                                                                                             attCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGACATCTCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGgcc                                                  >  2:659503/2‑149 (MQ=255)
                                                                                                  aGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATACCTCCCAGGGGGATGTGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACTGCGCACCGCTCCTCCACACGGCGGACCCCACCCCCGTACCCAGCGAGgcg                                                  >  1:392647/1‑143 (MQ=255)
                                                                                                         cAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGGGATAGGCCGCCAGAACCAgt                                      >  2:151919/1‑149 (MQ=255)
                                                                                                                             tgtTGGGGCATATCCCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTc                  <  2:84858/149‑1 (MQ=255)
                                                                                                                                 gggTCATATCTCCCAGGGCGATATGACCCAGTTCGCCTGCCACACCGGGTGCACCCGTCCACGGCGCACCGGTCATCCACCCTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGtttt                   <  1:401908/144‑1 (MQ=255)
                                                                                                                                    tCATATCGCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTcta                <  1:295516/144‑1 (MQ=255)
                                                                                                                                    gcctacctcCCAGGGGGATATGCCCCAGTTCGCCGGCCACACCGTGTGCCCCCGTCCACGGCGCACCGTTCAGCCACACTGCGAACCCCACCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTcta                <  1:11429/138‑1 (MQ=255)
                                                                                                                                      atatCCCCCTGGGAGATATGACCCAGTTCGCCTGCCACACCATGTGCACCCATCCCCGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTc         >  2:177819/1‑149 (MQ=255)
                                                                                                                                             ccAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGgag  <  2:66523/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
GGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAG  >  CP009273/4296968‑4297257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑