Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,297,1150GA21.9% 56.4 / 12.7 33P168S (CCC→TCC) alsKD‑allose kinase
Reads supporting (aligned to +/- strand):  ref base G (12/13);  new base A (2/5);  total (14/19)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTT  >  CP009273/4296974‑4297261
                                                                                                                                             |                                                                                                                                                  
tttCCGCATGGACGAAAAGATCGCCCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGa                                                                                                                                             <  2:110099/149‑1 (MQ=255)
gatctgCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGa                                                                                                                                             <  2:176128/144‑1 (MQ=255)
 ttCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGAc                                                                                                                                            <  2:570548/149‑1 (MQ=255)
    cGCATGGACGAAAAGATCGGGCAAGGGGTAATTTCGGAGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCa                                                                                                                                         <  2:231295/149‑1 (MQ=255)
      cATGGACGAAACGATCGCGCAATGGCTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGAc                                                                                                                                            <  1:353237/144‑1 (MQ=255)
      cATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCAATTCCAGAGCACTTGGTTTCCAGGCACCCAGGAATGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGAc                                                                                                                                            >  1:160627/1‑144 (MQ=255)
       tgggACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCGGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGtt                                                                                                                                      <  2:496174/147‑1 (MQ=255)
        tGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGAccc                                                                                                                                          <  1:11943/144‑1 (MQ=255)
         ggccGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCg                                                                                                                                    <  2:256157/146‑1 (MQ=255)
             gAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTg                                                                                                                                >  2:328837/1‑149 (MQ=255)
                                tAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACTCCGTGTGCAccaca                                                                                                             >  2:720698/1‑146 (MQ=255)
                                     tCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCAcc                                                                                                        >  2:346650/1‑148 (MQ=255)
                                         ggCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCAc                                                                                                         >  1:59984/1‑144 (MQ=255)
                                           cTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACgg                                                                                                       >  1:492960/1‑144 (MQ=255)
                                             gttgttCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGCTATGACCCAGTTCGCCTGCCCCACCGTGTGCACCCGTCCgcggcg                                                                                                     >  1:412111/1‑144 (MQ=255)
                                                        cAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGCCATATCTCCCAGGGGCATATGACCCAGTTCGCCTACCACACCGTGTGCACCCGTCCACGGCGCACCGTTCGTc                                                                                          >  1:34393/1‑144 (MQ=255)
                                                             tCTTAGCCCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGACATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGa                                                                                <  2:50821/149‑1 (MQ=255)
                                                               ttAGCGCCATTCCAGAGCAATTGGTTCCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAAc                                                                              <  2:14117/149‑1 (MQ=255)
                                                                    gcCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCAt                                                                         <  2:564329/149‑1 (MQ=255)
                                                                            agagCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTAc                                                                 >  2:312200/1‑149 (MQ=255)
                                                                             gagCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATAGCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTAcc                                                                <  2:338667/149‑1 (MQ=255)
                                                                                cagttGGTTTCGCGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCCTGTCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTa                                                                  <  1:183819/141‑1 (MQ=255)
                                                                                cAATTGGTTTCCAGGCACCCAGGATGGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTa                                                                  <  1:604152/144‑1 (MQ=255)
                                                                                   ttGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCAGCGTTCATCCACACTGCGAACCCCATCCCCGTACCg                                                               >  1:399181/1‑144 (MQ=255)
                                                                                       attCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGACATCTCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGgcc                                                      >  2:659503/2‑149 (MQ=255)
                                                                                            aGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATACCTCCCAGGGGGATGTGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACTGCGCACCGCTCCTCCACACGGCGGACCCCACCCCCGTACCCAGCGAGgcg                                                      >  1:392647/1‑143 (MQ=255)
                                                                                                   cAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGGGATAGGCCGCCAGAACCAgt                                          >  2:151919/1‑149 (MQ=255)
                                                                                                                       tgtTGGGGCATATCCCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTc                      <  2:84858/149‑1 (MQ=255)
                                                                                                                           gggTCATATCTCCCAGGGCGATATGACCCAGTTCGCCTGCCACACCGGGTGCACCCGTCCACGGCGCACCGGTCATCCACCCTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGtttt                       <  1:401908/144‑1 (MQ=255)
                                                                                                                              tCATATCGCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTcta                    <  1:295516/144‑1 (MQ=255)
                                                                                                                              gcctacctcCCAGGGGGATATGCCCCAGTTCGCCGGCCACACCGTGTGCCCCCGTCCACGGCGCACCGTTCAGCCACACTGCGAACCCCACCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTcta                    <  1:11429/138‑1 (MQ=255)
                                                                                                                                atatCCCCCTGGGAGATATGACCCAGTTCGCCTGCCACACCATGTGCACCCATCCCCGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTc             >  2:177819/1‑149 (MQ=255)
                                                                                                                                       ccAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGgag      <  2:66523/149‑1 (MQ=255)
                                                                                                                                           ggggATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGtt  >  2:595779/1‑149 (MQ=255)
                                                                                                                                           ggggATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGtt  >  2:94783/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                  
TTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTT  >  CP009273/4296974‑4297261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑