| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,420,470 | C→A | 100% | intergenic (‑203/+18) | hisJ ← / ← argT | histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,420,470 | 0 | C | A | 72.1% | 14.8 / 4.8 | 14 | intergenic (‑203/+18) | hisJ/argT | histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (3/1); new base A (3/7); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01 | |||||||||||
| Rejected as polymorphism: Not supported by required number of reads on both strands. | |||||||||||
GCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTCGTAGGTGCCGTCCTGACGCAGCTCGCCAAGCGCCTTATTGAAGGCAGCCGTCAG > CP009273/2420328‑2420589 | cgccgcagttgcgctggagaaggccagaaccagagagagcgatagcaccagttttttcataatgtcctcaactgacagtctttttatagggaatatttacaggtttgttacacttatcgtgccataaaaATTCCATCAGGTAAAGCTTc < 1:105789‑M2/20‑1 (MQ=255) caggtgcgctggagtaggccagaaccagagagagtgatagcaccagttttttcataatggcctcaactgacagtctttttatagggaatatttacaggtttgttacacttatcgtgccataaaaATTCCATCAGGTAAAGCTTCCCAGc < 2:268921‑M2/25‑1 (MQ=255) aaTTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGa > 2:55984/1‑149 (MQ=255) ggCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCAcc > 2:192823/1‑149 (MQ=255) gccagaaccagagagagcgatagcaccagttttttcataatgtcctcaactgacagtctttttatagggaatatttacaggtttgttacacttatcgtgccataaaaATTCCATCAGGTAAAGCTTCCCAGCGACGTATCAGTCACCGt < 1:282788‑M2/42‑1 (MQ=255) aaaaTATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTa < 2:279649/149‑1 (MQ=255) gagcgatagcaccagttttttcataatgtcctcaactgacagtctttttatagggaatatttacaggtttgttacacttatcgtgccataaaaATTCCATCAGGTAAAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCg < 2:246664‑M2/56‑1 (MQ=255) caccagttttttcataatgtcctcaactgacagtctttttatagggaatatttacatgtttgttacacttatcgtgccataaaaATTCCATCAGGTAAAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACttt > 2:175762‑M2/85‑149 (MQ=255) cggttttttcataatgtgctgaactgacagtctttttatagggaatatttacaggtttgttacacttatcgtgccataaaaATTCCATCAGGTAAAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTc < 1:165288‑M2/68‑1 (MQ=255) cataatgtcctcaactgacagtctttttatagggaatatttacaggtttgttacacttatcgtgccataaaaATTCCATCAGGTAAAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTg > 2:184664‑M2/73‑149 (MQ=255) ctttttatagggaatatttacaggtttgttacacttatcgtgccataaacATTCCATCAGGTAAAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTCGTAGGTGCCGTCCTGACGCa < 1:267245‑M2/99‑1 (MQ=255) aaTGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTCGTAGGTGCCGTCCTGACGCAGCTc > 2:272276/1‑149 (MQ=255) tgtttgttacacttatcgtgccataaaaATTCCATCAGGTAAAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTCGTAGGTGCCGTCCTGACGCAGCTCGCCAAGCGCCTTATTGaa < 1:175762‑M2/121‑1 (MQ=255) ttatcgtgccataaaaATTCCATCAGGTAAAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTCGTAGGTGCCGTCCTGACGCAGCTCGCCAAGCGCCTTATTGAAGGCAGCCgtccg > 2:124171‑M2/17‑147 (MQ=255) | GCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTCGTAGGTGCCGTCCTGACGCAGCTCGCCAAGCGCCTTATTGAAGGCAGCCGTCAG > CP009273/2420328‑2420589 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |