| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 821,679 | C→T | 18.4% | G344R (GGG→AGG) | ybhS ← | inner membrane putative ABC superfamily transporter permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 821,679 | 0 | C | T | 18.4% | 41.1 / 2.7 | 21 | G344R (GGG→AGG) | ybhS | inner membrane putative ABC superfamily transporter permease |
| Reads supporting (aligned to +/- strand): ref base C (9/8); new base T (2/2); total (11/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGACGAAATAACGAGCGGGAATAATGTACGTCACCGCGCGGATCACCGCGGGCATACTGTCGATCTGAAAAATAAAGCCGGAAAGCATAATCGACGGCAGAAAAGCGGCGT > CP009273/821533‑821816 | ttGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCgg > 1:56782/1‑149 (MQ=255) cGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGa < 2:288868/149‑1 (MQ=255) aTTAACGTCCATAATCGATGAAAGATGCTCTTCGCCCTAATCCAGCCAAGGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGa < 2:136218/149‑1 (MQ=255) cgcccATACGCCATGAAACACGCTCTTCTCCCTCATCCAGCCGACGTTTGCTGCTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAgg < 2:196263/146‑1 (MQ=255) tcttctCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGACGAAATa > 1:133396/1‑149 (MQ=255) cttctCCCTACTCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGACGAAATaa < 2:88775/149‑1 (MQ=255) cgtcGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGACGAAATAACGAGCGGGAATAATGTa < 1:103500/146‑1 (MQ=255) ccACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGACGAAATAACGAGCGGGAATAATGTACGTCACCGCGCGGATCa > 1:150895/1‑149 (MQ=255) aTAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGACGAAATAACGAGCGGGAATAATGTACGTCACCGCGCGGATCACCGCGGGCATACt < 1:168594/149‑1 (MQ=255) tAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCTGGCGAGGAACAGGCTTTGCAGGGTGCTGACGAAATAACGAGCGGGAATAATGTACGTCACGGCGCGGATCACCGCGGGCATACTg > 2:259417/1‑149 (MQ=255) tAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCTGGCGAGGAACAGGCTTTGCAGGGTGCTGACGAAATAACGAGCGGGAATAATGTACGTCACCGCGCGGATCACCGCGGGCATACTg > 2:259289/1‑149 (MQ=255) aCCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGACGAAATAACGAGCGGGAATAATGTACGTCACCGCGCGGATCACCGCGGGCATACTGTCGATCTg > 2:205042/1‑149 (MQ=255) cACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGACGAAATAACGAGCGGGAATAATGTACGTCACCGCGCGGATCACCGCGGGCATACTGTCGATCTGGAAAATAAAGCCGGAAAGCATag > 2:94545/1‑148 (MQ=255) gTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGACGAAATAACGAGCGGGAATAATGTACGTCACCGCGCGGATCACCGCGGGCATACTGTCGATCTGAAAAATAAAGCCGGAAAGCATAATCg > 2:233626/1‑149 (MQ=255) taacaccaGCACCACTGGCATATTCCCGGCGAGGAACAGGCGTTGCAGGGTGCTGACGAAATAACGAGCGGGAATACTGTACGTCACCGCGCGGATCACCGCGGGCATACTGTCGATCTGAAAAATAAAGTCGGAAAGCATAATCGACg < 1:151259/146‑1 (MQ=255) accacTGGAATATTCCCGGCGAGGAACAAGCTTTGCAGGGTGCTGACGAAATAACGAGCGGGAATAATGTACGTCACCGCGCGGATCACCGCGGGCATACTGTCGATCTGAAAAATAAAGCCGGAAAGCATAATCGACGGCAGAAAAgc > 2:187974/1‑149 (MQ=255) cacTGGAATATTCCTGGCGAGGAACAGGCTTTGCAGGGTGCTGACGAAATAACGAGCGGGAATAATGTACGTCACCGCGCGGATCACCGCGGGCATACTGTCGATCTGAAAAATAAAGCCGGAAAGCATAATCGACGGCAGAAAAgcgg < 1:259417/149‑1 (MQ=255) cacTGGAATATTCCTGGCGAGGAACAGGCTTTGCAGGGTGCTGACGAAATAACGAGCGGGAATAATGTACGTCACCGCGCGGATCACCGCGGGCATACTGTCGATCTGAAAAATAAAGCCGGAAAGCATAATCGACGGCAGAAAAgcgg < 1:259289/149‑1 (MQ=255) cTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGAGCTGACGAAATAACGAGCGGGAATAATGTACGTCACCCCGCGGAGCACCGCGGGCATACTGTCGAGCCGAAAAATAAAGCCGGAAAGCAACATCGACGGCCGAAGAggggcg > 1:94897/1‑149 (MQ=255) tGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGACGAAATAACGAGCGGGAATAATGTACGTCACCGCGCGGATCACCGCGGGCATACTGTCGATCTGAAAAATAAAGCCGGAAAGCAAAATCGACGGCAGAAAAacggcga > 1:238668/1‑148 (MQ=255) tGGAATATTCCCGGCGAGGAACAGGCTGTGCAGGGTGCTGACGAAATGACGAGCGGGAATAATGTACGTCACCGCGCGGATCACCGCGGGCATACTGTCGATCTGAAAAATAAAGCCGGAAAGCATAATCGACGGCAGAATGGTGGCGt < 1:192106/149‑1 (MQ=255) | TTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGACGAAATAACGAGCGGGAATAATGTACGTCACCGCGCGGATCACCGCGGGCATACTGTCGATCTGAAAAATAAAGCCGGAAAGCATAATCGACGGCAGAAAAGCGGCGT > CP009273/821533‑821816 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |