Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 468,126 Δ1 bp coding (73/339 nt) glnK → nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273468,1260A.98.1% 462.4 / ‑1.0 103coding (73/339 nt)glnKnitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base . (44/57);  minor base C (0/2);  total (44/59)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

TTTCTGGCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAA  >  CP009273/467982‑468269
                                                                                                                                                 |                                                                                                                                               
tttCTGGCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGACGCGTTATCTTCCAGTGGTTTCAg                                                                                                                                            >  1:700313/1‑149 (MQ=255)
 ttCTGGCACACCGCTTGCAGTACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAgg                                                                                                                                           <  1:1413007/149‑1 (MQ=255)
      gCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTGATCTTCCATTGGTTTCAGGGCCTg                                                                                                                                      >  1:42590/1‑149 (MQ=255)
       cacaCCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGa                                                                                                                                     <  1:673354/149‑1 (MQ=255)
        acacCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGAGCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGAc                                                                                                                                    >  1:1824523/1‑149 (MQ=255)
         cacCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGc                                                                                                                                         <  2:1291498/143‑1 (MQ=255)
            cGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGAGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTg                                                                                                                                      >  2:306698/1‑143 (MQ=255)
             gCTTGCAATACCTTCTTCGTGTGGCAGAGCCATTACCGAAGTCTGACCGGAGGGGATCTATGAGGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTGCATTGGTTTCAGGGCCTGACCGTCa                                                                                                                               <  1:963011/149‑1 (MQ=255)
              cTTGCAATACTTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCAc                                                                                                                              <  1:1516018/149‑1 (MQ=255)
                 gCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGt                                                                                                                                 <  2:1628158/143‑1 (MQ=255)
                 gCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGt                                                                                                                                 <  2:852660/143‑1 (MQ=255)
                  cagtACCTTCTTCGTGTCGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCATGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGaa                                                                                                                          <  1:1373715/146‑1 (MQ=255)
                  cAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGaa                                                                                                                          <  1:578981/149‑1 (MQ=255)
                  cAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGaa                                                                                                                          <  1:834413/149‑1 (MQ=255)
                   actgcctcgttcGTGTAGCAGAACCATTACCGAATGCTGACCGGAGGGGATCCACGAAGCTGGTGACCGTGATAATCACACCATTCATGCCGGAAGCCGTTCGCGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCa                                                                                                                               <  2:305524/134‑1 (MQ=255)
                   aaTACCTTCTTCGTGTAGCAGAACCATTCCCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCa                                                                                                                               <  2:1074451/143‑1 (MQ=255)
                     tACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCACGCTGGAAGACGTGCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCAcc                                                                                                                             >  2:622460/1‑143 (MQ=255)
                        cttcttCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGGCCGTGATAATCAAACAATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTGCCATTGGTTTCAGGGCCGGACCGTGACCGaa                                                                                                                          >  2:1720937/1‑143 (MQ=255)
                        cttcttCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGaaa                                                                                                                    <  1:756562/149‑1 (MQ=255)
                        cttcttCGTGTAGCAGAACCATCACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGaaa                                                                                                                    <  1:131995/149‑1 (MQ=255)
                         ttcttcGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAg                                                                                                                   <  1:75195/149‑1 (MQ=255)
                         ttcttcGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAg                                                                                                                   >  1:79789/1‑149 (MQ=255)
                             tcGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATGATCAAACCATTCAAGCTGGAAGACGTTCGTGGAGCGTTATGTTCCCTTGGTTTCAGGGCCTGACCGTCTCCGAAGTGAAAGGttt                                                                                                               >  1:425544/1‑149 (MQ=255)
                              cgttgaGCACAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGaaa                                                                                                                    <  2:1470128/138‑1 (MQ=255)
                               gtgtAGCAGAACCAGTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTGCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAg                                                                                                                   <  2:1158819/143‑1 (MQ=255)
                                gttAGCAGAACCATTACCGAATTCTGACCGGAGGCGCTCCATGACGCTGCGGACCGTGATAATCAATCCCAGCCATCTGGAAGCACGTTGTGATACTTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCgg                                                                                                            <  1:927298/147‑1 (MQ=255)
                                 gtAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCggg                                                                                                           >  1:1141165/1‑149 (MQ=255)
                                  tAGCAGAACCATTACCGAATTCTCACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGtt                                                                                                                >  2:1191801/1‑143 (MQ=255)
                                    gCAGATCCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGt                                                                                                        <  1:845187/149‑1 (MQ=255)
                                    gCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGGTTCGGGCGt                                                                                                        >  1:333434/1‑149 (MQ=255)
                                        aaCCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGa                                                                                                    >  1:1435044/1‑149 (MQ=255)
                                        aaCCAGTACCGAATTCTGACCGGGGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGa                                                                                                    <  1:240025/149‑1 (MQ=255)
                                          ccATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGt                                                                                                        <  2:1730155/143‑1 (MQ=255)
                                          ccATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATGGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGt                                                                                                        <  2:721078/143‑1 (MQ=255)
                                          ccATTACCGAATTCTGACCGGAGGGGATCTATGAAGCAGGTGACCGTGATAATCAAACCATTCATGCTGGAAGACGTCCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGt                                                                                                        <  2:1730145/143‑1 (MQ=255)
                                          ccATTACCCAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGt                                                                                                        <  2:1758212/143‑1 (MQ=255)
                                           cATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAg                                                                                                 >  1:1206942/1‑149 (MQ=255)
                                           cATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAg                                                                                                 <  1:1642935/149‑1 (MQ=255)
                                             ttACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAggg                                                                                               <  1:1272526/149‑1 (MQ=255)
                                              tACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGc                                                                                              >  1:1776561/1‑149 (MQ=255)
                                               aCCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACAATTCAAGATGGAATACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCAGCGAAGTGTAAGGTTTCGGTCGTCAGAAAGGGCa                                                                                             >  1:1167368/1‑149 (MQ=255)
                                                ccGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCGGAAAGGGCAt                                                                                            >  1:1551630/1‑149 (MQ=255)
                                                ccGAATGCTGACCGGAGGGGATCTATGAAGCTGGTAACCGTGATAATCAAACCATTCCAGCTGGAAGACGTTCGTGAAGCGTTATCGTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCAt                                                                                            <  1:1601698/149‑1 (MQ=255)
                                                 cGAATTCTGACCGGAGGGGCTATCGGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAg                                                                                                 <  2:1687757/143‑1 (MQ=255)
                                                 cGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAg                                                                                                 >  2:671081/1‑143 (MQ=255)
                                                      tCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAg                                                                                      <  1:1631598/149‑1 (MQ=255)
                                                       cTGACCGGAGGGGATCTATGAACACGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATg                                                                                           <  2:1404269/143‑1 (MQ=255)
                                                            cGGGGGGGATCTATGAAGCTGTGGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGGTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAg                                                                                      <  2:572767/143‑1 (MQ=255)
                                                              gAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCt                                                                                    >  2:1603202/1‑143 (MQ=255)
                                                                ggggTTCTATGAAGCTGGTGGCCGGGATAATCAAACCATTCTAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGGCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCggg                                                                            <  1:737971/149‑1 (MQ=255)
                                                                ggggATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCggg                                                                            <  1:260607/149‑1 (MQ=255)
                                                                   gATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAGGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTAcc                                                                               >  2:884918/1‑143 (MQ=255)
                                                                   gATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAGGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGGGCTGTACCgggggg                                                                         >  1:597865/1‑149 (MQ=255)
                                                                   gATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTAcc                                                                               >  2:1737991/1‑143 (MQ=255)
                                                                   gATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCgggggg                                                                         >  1:913946/1‑149 (MQ=255)
                                                                    aTCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAGGTGAAAGGTTTCGGGCGTCAGAAAAGGCATGCCGAGCTGTACCGGGGGGc                                                                        >  1:1042189/1‑149 (MQ=255)
                                                                      cTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCgg                                                                      >  1:1756914/1‑149 (MQ=255)
                                                                       gttGAAGCTGGGTGCCGTGATAATCAATCCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCgggg                                                                           <  2:1251159/141‑1 (MQ=255)
                                                                        aTGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGaa                                                                    <  1:333310/149‑1 (MQ=255)
                                                                          gAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGc                                                                        >  2:547334/1‑143 (MQ=255)
                                                                           aaGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATAc                                                                 <  1:1098660/149‑1 (MQ=255)
                                                                            aGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTTACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGCGCCGAATACa                                                                <  1:468129/149‑1 (MQ=255)
                                                                            aGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACa                                                                >  1:1195863/1‑149 (MQ=255)
                                                                              cTGGTGACCGTTATAATCAAACCATCCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGGGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGaa                                                                    <  2:1568226/143‑1 (MQ=255)
                                                                              cTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGaa                                                                    >  2:1734579/1‑143 (MQ=255)
                                                                               tGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGACAGGTTTCGGGCGGCAGAAAGGGCATGCCGAGCTGTACAGGGGGGCGGacg                                                                   >  2:614323/1‑141 (MQ=255)
                                                                                ggggACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATa                                                                  <  2:306811/140‑1 (MQ=255)
                                                                                      cGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGt                                                            >  2:371047/1‑143 (MQ=255)
                                                                                            aaTCAAACCATTCAAGCTGGAAGACGTTCGTAAAGCGTCATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGTGACGGAATACAGCGTCAAttg                                                      >  2:1687996/1‑142 (MQ=255)
                                                                                                aaaCCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTg                                                  >  2:266323/1‑143 (MQ=255)
                                                                                                aaaCCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCaaaa                                            >  1:1325460/1‑149 (MQ=255)
                                                                                                 agcccatcagtcTGGACGACGTTCGTGAAGCTCTATCTGCCATTGGTTTCAGGGCCGGACCGTCACCGCAGTGAAAGGTTGCGTGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTCCCTGc                                                 <  2:499780/132‑1 (MQ=255)
                                                                                                 aaCCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAg                                           >  1:1490188/1‑149 (MQ=255)
                                                                                                 aaCCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAg                                           >  1:1518004/1‑149 (MQ=255)
                                                                                                      ttCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGTATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTaaaa                                      <  1:1687804/149‑1 (MQ=255)
                                                                                                         aaGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTa                                         <  2:1040296/143‑1 (MQ=255)
                                                                                                          aGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTg                                  <  1:1551999/149‑1 (MQ=255)
                                                                                                          aGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTg                                  >  1:1687791/1‑149 (MQ=255)
                                                                                                          aGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTg                                  <  1:1432861/149‑1 (MQ=255)
                                                                                                            cTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCCCCGCAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGCCAACTTCCTCCCAAAAGTaaaa                                      >  2:50980/1‑143 (MQ=255)
                                                                                                               gAAGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCACAAGTAAAAAtt                                   >  2:547260/1‑143 (MQ=255)
                                                                                                                aaGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTgg                            <  1:1499742/149‑1 (MQ=255)
                                                                                                                aaGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAAATg                                  >  2:353227/1‑143 (MQ=255)
                                                                                                                aaGACGTTCGTGAAGCGTTATCTTCCATTGGCTACAGGCCCGCACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTgg                            <  1:997099/149‑1 (MQ=255)
                                                                                                                 aGACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGa                                 >  2:913181/1‑143 (MQ=255)
                                                                                                                  gACGTTCGTGAAGCGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCg                          <  1:360063/149‑1 (MQ=255)
                                                                                                                       ttCGTGAAGCGTTATCATCCATTGGTTTCAGGGCCTGGCCGTCACCGAGGTGAAAGGGTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTg                      >  1:1212871/1‑149 (MQ=255)
                                                                                                                        tCGTGAAGCGTTATCTTCCATTGGCTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGc                     <  1:514647/149‑1 (MQ=255)
                                                                                                                          gTGAAGCGTTATCTTCCATTGGTTTCAGGGCCGGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGa                         <  2:1317861/143‑1 (MQ=255)
                                                                                                                             aaGCGTTATCCTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCtgatg                <  1:1484650/149‑1 (MQ=255)
                                                                                                                              aGCGTTATCTTCCATTGGTTTCAGGGCCGGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGc                     <  2:38381/143‑1 (MQ=255)
                                                                                                                              aGCGGTCTCTTCCCTTGGTTCCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGc                     <  2:824338/143‑1 (MQ=255)
                                                                                                                                cGTTCTCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGCGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCtg                   <  2:1112/143‑1 (MQ=255)
                                                                                                                                cGTTATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAACTGATGTGGCGATTGCTGATGAcc             <  1:1007877/149‑1 (MQ=255)
                                                                                                                                  ttATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCtgat                 <  2:1103071/143‑1 (MQ=255)
                                                                                                                                  ttATCTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCtgat                 >  2:752950/1‑143 (MQ=255)
                                                                                                                                      cTTCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCg       <  1:332057/149‑1 (MQ=255)
                                                                                                                                       ttCCCTTGGTTGCAGGCGCTGCCCGTCCGCGAAGGTAAAGGTTTTGGGCGTCAGAAAGGGTATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGa      <  1:1817338/149‑1 (MQ=255)
                                                                                                                                       ttCCATTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGa      >  1:1794990/1‑149 (MQ=255)
                                                                                                                                           aTTGGTTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGaa  >  1:413390/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TTTCTGGCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAA  >  CP009273/467982‑468269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: