Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,208,0190AG21.7% 233.6 / 26.1 116E538E (GAA→GAG) rpoDRNA polymerase, sigma 70 (sigma D) factor
Reads supporting (aligned to +/- strand):  ref base A (52/38);  new base G (0/25);  total (53/63)
Fisher's exact test for biased strand distribution p-value = 2.14e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 118 total aligned reads displayed.

TGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCC  >  CP009273/3207871‑3208167
                                                                                                                                                    |                                                                                                                                                    
tGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGGTGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGaa                                                                                                                                                      >  1:19445/1‑149 (MQ=255)
   cGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGc                                                                                                                                                   >  1:806871/1‑149 (MQ=255)
        gACAAGATCCGCAAAGTGCTGAAGATCGCGAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGCAGATTCGCATCTGGGGGGTTTCAGCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGCCCCCCGAAAg                                                                                                                                                    >  2:1657149/1‑143 (MQ=255)
           aaGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCtgcgtgc                                                                                                                                           <  1:1424107/149‑1 (MQ=255)
           aaGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAAGGTACCACCCGCGAGCTGGCGTTGTATTCTGCGGCCACCGACAGCCtgcgtgc                                                                                                                                           >  1:1673937/1‑149 (MQ=255)
             gATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCgg                                                                                                                                         <  1:1105937/149‑1 (MQ=255)
             gATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTATGCGCCCACCGGAAGCCTGCGTGCgg                                                                                                                                         >  1:507467/1‑149 (MQ=255)
             caTCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCAAGGAAACGCCGAACAGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACGCCCCTCGAGCTGCCTCTGGATGCTGCGACCACCGAAAGACtgg                                                                                                                                               >  2:233495/2‑142 (MQ=255)
              aTCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCACGGAAACGCCGATCGGTGATGATGAAGATTCGCACCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGc                                                                                                                                        <  1:1518657/149‑1 (MQ=255)
               tCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCAACCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCtgcgc                                                                                                                                             >  2:1093536/1‑142 (MQ=255)
                     gaGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGGTCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCac                                                                                                                                 <  1:1430074/148‑1 (MQ=255)
                      aGTGCTTAAGATCGCCAAAGCGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCAGCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCacg                                                                                                                                <  1:1786783/149‑1 (MQ=255)
                         gCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAGGCCtgcgtgcgttgccgcacgcag                                                                                                                             <  1:1422608/149‑14 (MQ=255)
                             taGATCGCCTAAGAGGCAATCTCCCTGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGCTACCACCCTTTTTTTTTCTCATGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCacga                                                                                                                               <  2:549618/142‑1 (MQ=255)
                             aaGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGCTTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTgct                                                                                                                         >  1:945401/1‑149 (MQ=255)
                             aaGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTgct                                                                                                                         >  1:142159/1‑149 (MQ=255)
                                    ccAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGGgccg                                                                                                                        >  2:1572425/1‑141 (MQ=255)
                                      aaaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCt                                                                                                                >  1:1652410/1‑149 (MQ=255)
                                          ggCCAATCTCCATCGAAACGCCGATCGGGGATGAGTAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTTTTTTTTTTTAAGGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCtggctggc                                                                                                                  <  2:503557/142‑1 (MQ=255)
                                           gCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCCACGCCCGACGGCctggccgggc                                                                                                                 >  2:1428658/1‑141 (MQ=255)
                                            ccAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCCCGCCGTGCTGCCTGGccc                                                                                                                >  2:869076/1‑142 (MQ=255)
                                             cAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCGGCGAGCGGCGACGGACGGCGTgctggtcgggcgt                                                                                                               >  2:1614729/1‑135 (MQ=255)
                                              agtctcCGTGGAAACGCCGATCGGTGATGATGTAGATTCGCATCTGGGGGATTTCATCGAGGATATCACCCTTTTGTTTTTTAAGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCGGGCTGGCCTGa                                                                                                              <  2:1525304/141‑1 (MQ=255)
                                              aaTCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCATGACGTGCTGGCTGGCCTGACcgcgc                                                                                                        >  1:1158427/1‑149 (MQ=255)
                                              aaTCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACcgcgc                                                                                                        <  1:306558/149‑1 (MQ=255)
                                                tctcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGt                                                                                                      <  1:862473/149‑1 (MQ=255)
                                                   ccATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGCGCGGGCCGGCCTGCCcgcg                                                                                                         >  2:1369140/1‑143 (MQ=255)
                                                   ccAAGGAACCGCCGATCGGGGATGATGAAGATTCGCATCTGGCGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCATCGAAAGCCTGCGTGCGGCAACGCACCACGTGCTGGCTGGCCTGACCGCGCGTGaa                                                                                                   <  1:1345368/149‑1 (MQ=255)
                                                    cctGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTAGGGGATTTCCGCGGGGATACCACCCGCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCCACGAACGACGTGCTGGCAGGCCCGTTCGCGtgtggtg                                                                                                  >  1:1602880/3‑146 (MQ=255)
                                                     atggctgggCCGGTCGGTGTTGGTGACGATTCGCCTCTGGGGGATTGCATCGAGGTTACCACCCTTTGTTTGTCTCTTGATCCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACcgcgcg                                                                                                       <  2:842982/135‑1 (MQ=255)
                                                       ggAAGCGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGAGTTCATCGAGGATACCACCCTCGAGCTGCCGATTGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTg                                                                                                     <  2:1465683/143‑1 (MQ=255)
                                                       ggAACCGCCGATCGGTTATGATGAAGACTCGCATCTGGGGGATTTCATCGCGGATCCCCCCCTTTTGTTTTTGCAGGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTg                                                                                                     <  2:1052192/143‑1 (MQ=255)
                                                        gAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGCGGATACCACCCTAGGGCGGCCGCTGGATTCTGCGAACACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGGCCGCGAGTGAAGCaaa                                                                                              >  1:361501/1‑149 (MQ=255)
                                                           aCGCCGATCGGTGATGATGATGATTCGCATCTGGGGGATTTCATCGGGGATACCACCCTCGTGCTGTCGCATGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGc                                                                                                 <  2:118535/143‑1 (MQ=255)
                                                             gCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTc                                                                                         >  1:942936/1‑149 (MQ=255)
                                                             gCCGACCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACTCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTc                                                                                         <  1:668006/149‑1 (MQ=255)
                                                              ccGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCt                                                                                        <  1:252609/149‑1 (MQ=255)
                                                                catgggTGAGGATGAAGATTCTGATCTGTGGTGTTTCCTCGAGGATACCACCCTTTTTTTTTTTAAGGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAg                                                                                            <  2:1369183/139‑1 (MQ=255)
                                                                    ggggatgttgaAGATTCGCATCGGGGGGATTTCATCGAGGATACCACCCTCGTGTTGTCGATTGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCt                                                                                        <  2:1008673/140‑1 (MQ=255)
                                                                    ggggatgatgtGGCTGCGCTTCTGGGGGAGTTCATCGAGGATACCACTCTTGTGTTTTCGCATGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCt                                                                                        <  2:506025/140‑1 (MQ=255)
                                                                    ggTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCTTGTTTTCGCATGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCt                                                                                        <  2:1087523/143‑1 (MQ=255)
                                                                      tggtgttgaAGTTTCGTATCTGGGGGATTTCATCGAGGTTGCCGCTTTTGTGTTGTGTGTGGATGCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGc                                                                                      <  2:1040701/137‑1 (MQ=255)
                                                                      tgatgatgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGc                                                                                >  1:1454222/1‑149 (MQ=255)
                                                                          gaggaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTCAAGCAAAAGTTCTGCGTATGCGttt                                                                            <  1:960734/146‑1 (MQ=255)
                                                                           atgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTc                                                                           >  1:859398/1‑149 (MQ=255)
                                                                            tgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGCAGTGCTGGCTGGGCTGACCGCGCGTGCGGCCAAAGAGCTGCGCATGc                                                                                >  2:442846/1‑143 (MQ=255)
                                                                            cgcaGATCCGCATCTGGCTGATTTCATCGAGGATACCCCCCTCGAGCCGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCg                                                                          <  1:966689/146‑1 (MQ=255)
                                                                             tgagtctCGCATGTTGGGGATTTCATCGAGGATACCCCCCTGGTGTTGCTGCAGGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCg                                                                               <  2:1551299/137‑1 (MQ=255)
                                                                              aaGATTCGCATCTGGGGGATTTCATGGAGGATACCACCTTCGTTTTGTTTCTGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGt                                                                              <  2:1569411/143‑1 (MQ=255)
                                                                              aaGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGGGAGGCAAAAGTTCTGCGTCTGCGTTTCggg                                                                        >  1:33780/1‑148 (MQ=255)
                                                                                 gttCGCATCTGCGGTATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATc                                                                     <  1:13083/148‑1 (MQ=255)
                                                                                  ttCGCATCCGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCg                                                                    <  1:195936/149‑1 (MQ=255)
                                                                                    cGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGCAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGat                                                                  <  1:1885702/149‑1 (MQ=255)
                                                                                    cGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGat                                                                  <  1:1573496/149‑1 (MQ=255)
                                                                                     gCATCTGGGGCATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGGGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGata                                                                 <  1:1852488/149‑1 (MQ=255)
                                                                                      cagcTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGatat                                                                <  1:854714/146‑1 (MQ=255)
                                                                                       aTCTGGGGGATTTCATCGAGGATACCACCGTCGTTCTTCTGATGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATc                                                                     <  2:33827/143‑1 (MQ=255)
                                                                                         cTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGaa                                                             <  1:1698376/149‑1 (MQ=255)
                                                                                         cTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGaa                                                             <  1:1676619/149‑1 (MQ=255)
                                                                                          tGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGGATCGATATGacc                                                            >  1:845266/1‑147 (MQ=255)
                                                                                             gggATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACAcc                                                         >  1:8168/1‑149 (MQ=255)
                                                                                             gggATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACAcc                                                         <  1:1037410/149‑1 (MQ=255)
                                                                                              ggATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGCAAGCCTGCGTGCGGCAACACCCGACGTGCTGGCTGGCCTGACCGCGTTAATTTTAAATTTTCTGCGTATGCGTTTCGGTATCGATCTGAACACCg                                                        >  1:626321/1‑149 (MQ=255)
                                                                                                  ttCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACCACGACtc                                                    >  1:656306/1‑148 (MQ=255)
                                                                                                  ttCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTa                                                    >  1:892421/1‑149 (MQ=255)
                                                                                                  ttCAGCGAGGATACCACCCCCGAGCTGCCGCTGGATTCGGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTa                                                    <  1:211674/149‑1 (MQ=255)
                                                                                                     aTCGAGGATACCACCCTCGAGCTGCCGCTCGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCAGTATCGATATGAACACCGACTacac                                                 >  1:1162662/1‑149 (MQ=255)
                                                                                                      ttgtggttaccctccTCGTGTTTCCGCATGATTCGGCGACCACCGAGTGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGAc                                                      <  2:525951/130‑1 (MQ=255)
                                                                                                      tCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAACCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAAAACCGACTACACg                                                >  1:1068268/1‑149 (MQ=255)
                                                                                                       gcaGGATACATCCCTTGATTTGCTGAAGGATTCGGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACt                                                     <  2:1326850/141‑1 (MQ=255)
                                                                                                        gAGGTTCCCCCCCTCGAGCTGCCGCTGTATTCTGCGGCCAGCTAAAGCGTGCGTGCGGCAACGAACGACGGGCTGGCTGGCCTGCCCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCt                                              <  1:527060/149‑1 (MQ=255)
                                                                                                           gATACCACCCTCGGGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGga                                           >  1:1298507/1‑149 (MQ=255)
                                                                                                              cccaccCTTGTTCTGTTGCAGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCt                                              <  2:211210/142‑1 (MQ=255)
                                                                                                              accaccATCGAGCGGCCGCGGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGgaaga                                        <  1:149776/149‑1 (MQ=255)
                                                                                                                 accCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGt                                     <  1:1780291/149‑1 (MQ=255)
                                                                                                                  cccTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAAAACCGACTACACGGTGGAAGAAGTg                                    >  1:1076507/1‑149 (MQ=255)
                                                                                                                   ccTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTgg                                   <  1:1706721/149‑1 (MQ=255)
                                                                                                                    cTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTggg                                  >  1:404239/1‑149 (MQ=255)
                                                                                                                       gtgCTTCCGCTGGATTCTGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGt                                     <  2:1340739/141‑1 (MQ=255)
                                                                                                                        agttGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTg                                    <  2:129879/140‑1 (MQ=255)
                                                                                                                         tttGCCGGCGGATACGGGGACCGCCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTgg                                   <  2:1360127/141‑1 (MQ=255)
                                                                                                                             ccGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGAAAAAGTTCTGAGTCTACGTTTCGGTCTAGCTATAAGCACCGACTGGATGCTAGTAGAAATGGGtgc                               >  2:1206430/1‑141 (MQ=255)
                                                                                                                             ccGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCACGTGAAGCAAAAGTTCTGCGTATGCGGTTCGGTATCGATATGAAGACCGACTACCCCCCGGAAGAAGCGGGTaa                               >  2:1341308/1‑143 (MQ=255)
                                                                                                                               gCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGACAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGCTCg                       >  1:248105/1‑149 (MQ=255)
                                                                                                                                    aTTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTAGCTGACCTGACCGCGCGTGAAGCAAAGGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGCAGAAGTGGGTAAACAGTTc                        >  2:627419/1‑143 (MQ=255)
                                                                                                                                     ttCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACATGCTGGCTGGCCCGATCGCGCGTGAAGCAAAAGTTCTGCGTCTGCGTTTCGGTATCGATATGAACACCGCCTACACGCTGGAAGAAGTGGGTAAACCGTTCg                       >  2:349787/1‑143 (MQ=255)
                                                                                                                                     ttCTGCGAACACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCAACTCACTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTa                 >  1:1390579/1‑149 (MQ=255)
                                                                                                                                       cTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGAc                     <  2:852629/143‑1 (MQ=255)
                                                                                                                                       cTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTAcc               >  1:1302392/1‑149 (MQ=255)
                                                                                                                                        tGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGGTAccc              >  1:971998/1‑149 (MQ=255)
                                                                                                                                           gACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCACAAGTTCTGCGTATGCGTTTCGGTATCGCTATGAACACCGGCTACACGCTGGAAGACGTGGGTAAACAGTTCGACGTTa                 >  2:187551/1‑143 (MQ=255)
                                                                                                                                            accaccGAAAGCCTGCGTGCGGCGACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTCCCCGCGa          >  1:1049245/1‑149 (MQ=255)
                                                                                                                                             ccaccGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGaa         >  1:617033/1‑149 (MQ=255)
                                                                                                                                              caccGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAAAAGTTCGACGGTACCCGCGAAc        >  1:1607682/1‑149 (MQ=255)
                                                                                                                                               accGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTGAACAGTTCGACGTTACCCGCGAACg       >  1:227652/1‑149 (MQ=255)
                                                                                                                                               accGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGATGGAAGAAGTGGGTAAACAGTTCGACGTTAACcg             >  2:513120/1‑143 (MQ=255)
                                                                                                                                                ccGAAAGTCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGTTAAACAGTTCGACGTTACCCGCGAACGt      <  1:381521/149‑1 (MQ=255)
                                                                                                                                                 cGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTa     >  1:705098/1‑149 (MQ=255)
                                                                                                                                                 cGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCATTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTAGACGTTACCcgcg           >  2:671381/1‑143 (MQ=255)
                                                                                                                                                    aaGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGGGATGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTCCGACGTTACCCGCGAACGTATcc  <  1:1005550/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCC  >  CP009273/3207871‑3208167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑