Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | CP009273 | 2,420,328 | G→A | intergenic (‑61/+160) | hisJ ← / ← argT | histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | CP009273 | 2,420,328 | 0 | G | A | 98.0% | 137.8 / NA | 51 | intergenic (‑61/+160) | hisJ/argT | histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit |
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (28/22); minor base C (1/0); total (29/22) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 |
TTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGTGCCAT‑‑‑‑A‑‑‑‑AAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCAT > CP009273/2420189‑2420463 | tttGTGGAATCGCAGCACACGCCGTAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCTTAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGTACCATaaaaa < 1:216742‑M1/149‑6 (MQ=255) tttGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGTACCATaaaca > 1:240605‑M1/1‑144 (MQ=255) cGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGTACCATaaaaaaaat > 1:761081‑M1/1‑140 (MQ=255) ggAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGTCCCATaaaaaaaatt > 2:68701‑M1/1‑139 (MQ=255) ggAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGTACCATaaaaaaaatt > 1:95457‑M1/1‑139 (MQ=255) acacgcaGCAAACGCCGCAGTTGCGCGGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGTACCATaaaaaaaattga < 1:350855‑M1/146‑13 (MQ=255) aaTCGCAGCAAACGCCGCAGTTGCGACGGCGAAGGCCAGAACCAGAGAGAGCTATAGCATCAGTGTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGTACCATaaaaaaaattga < 2:2643‑M1/149‑13 (MQ=255) tCGCAGCAACCGCCGCCATTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTGTTTATAGGGAATATGT‑ACGGGTTTGTTACACTTGTCGCACCATgaaaaaacttgacc > 2:185971‑M1/1‑135 (MQ=255) cagcaAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATCATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGTACCATaaaaaaaattaaccctc > 1:688977‑M1/1‑132 (MQ=255) agcaAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGTACCATaacaaaaattgaccatca > 1:36476‑M1/1‑131 (MQ=255) caAACGCCGCAGTTGCCCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATCATGTACTCAACTCCAACTCTTTCTATATGGAATATTTGACA‑ATACTTTACACTTATCGTACCATAACAA‑‑‑‑AAATTGACCATCAAg < 2:917969/149‑1 (MQ=255) aaaCGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTGTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTAACGTACCATaaaaaaaattgaccatcaagg > 2:610806‑M1/1‑128 (MQ=255) ccgcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGTACCATaaaaaaaattgaccatcaaggcaaaa < 2:863721‑M1/149‑27 (MQ=255) ccgcAGTTGCGCTGGACAATGCCAGAACCAGAGAGAGCGATAGCACCAGTTGTTTCACAATGTCCTCAACTGACAGTCTTTCTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGTACCATaaaaaaaattgaccatcaaggcaaaa < 2:250378‑M1/149‑27 (MQ=255) cgcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCCACTAGCAGTCTTTTTATAGGGAAGATTT‑CCAGGGTTGTTACACTTATCGTACCATaaaaaaaattgaacatccgagcaaaaa > 1:451165‑M1/1‑122 (MQ=255) gcTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGTACCATaaaaaaaattgaccatcaaggcaaaatatcccgccc > 1:503299‑M1/1‑113 (MQ=255) aaGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTATTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGTACCATaaaaaaaattgaccatcaaggcaaaatatcccgccccgttaga < 1:111660‑M1/149‑44 (MQ=255) aGGACAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGTACCATaaaaaaaattgaccatcaaggcaaaatatcccgccccgttagac < 1:455109‑M1/149‑45 (MQ=255) gCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGTACCATaaaaaaaattgaccatcaaggcaaaaaatcccgccccgttagacgt > 2:368002‑M1/1‑103 (MQ=255) cAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGTACCATaaaaaaaattgaccatcaaggcaaaatatcccgccccgttagacgtat > 1:45520‑M1/1‑101 (MQ=255) gcaccATAGAGATCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGTACCATaaaaaaaattgaccatcaaggcaaaatatcccgccccgttagacgtatta < 2:761081‑M1/147‑51 (MQ=255) aaCCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGTACCATaaaaaaaattgaccatcaaggcaaaatatcccgccccgttagacgtattat > 2:447759‑M1/1‑98 (MQ=255) ccAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGTACCATaaaaaaaatggaccatcaaggcaatatatcccgccccgttagacacatgtttt > 1:537205‑M1/1‑96 (MQ=255) gggagagcgATAGAACCAGTTTTCTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGTACCATaaaaaaaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttc < 1:190330‑M1/147‑57 (MQ=255) gagagcgCTATAGCGCCTGTTTTTGCAGACTGGCCTCAGCTGACAGTCTTTTTCTAGGGAATATTT‑ACAGGTTTGTTTCACTTATCATACCATaaaaaaaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttc < 2:319099‑M1/149‑57 (MQ=255) gagagagCGATCGCACCAGTTTTTTCATATTGTCCTCAACTGCCAGTCTTTATAACACGGTTAATG‑ACAGGTTTGTTACACTTATCGTACCATaaaaaaaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttc < 1:938091‑M1/149‑57 (MQ=255) gagagagCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT‑CCAGGTATGTTACACTTATCGTACCATaaaaaaaattgaccatcaagccaaaatatcccgccccattagacgtattatttttc > 1:821554‑M1/1‑93 (MQ=255) gagagagCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGTACCATaaaaaaaattgaccatcaaggcaaaatatcccgccccgttggacgtattatttttc > 1:214014‑M1/1‑93 (MQ=255) gagagagCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGTACCATaaaaaaaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttc > 1:214035‑M1/1‑93 (MQ=255) agggggcgataGCACCAGTTTTTTCATAATGGTCGCAACTGACAGTCTTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGTACCATaaaaaaaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttca < 2:75768‑M1/144‑58 (MQ=255) agagagCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGTACCATaaaaaaaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttca < 2:548829‑M1/149‑58 (MQ=255) agCGATAGCACCAGTTTTTTCATTTTTTTTTCAACCGAAAGTCTGCCTATAGGAAATATTT‑ACAGGTTTGTTGCACTCACCGTACCAT‑‑‑‑AAAAGAAATTGACCATCAAACCAAAATATCCTCCCCCTTTACACGTATAATTCTTCATCaa > 2:164812/1‑149 (MQ=255) agagtgcatgagttttttaatATTGTCCTGAACTGACAGTCTTTTTATAGGTAATTTTT‑ATATGTTTGTTACACTTATCGTACCATaaaaaaaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaa < 2:955897‑M1/139‑64 (MQ=255) cACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGGAACATaaaaaaaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgctt < 1:76907‑M1/149‑70 (MQ=255) aCCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT‑ACAGGCTTGTAACACGTATCGTATCTGataagaaattgaccatcaaggcaaaagagcccgccccgttagacgtattatttttcattaaaaatgctta > 1:942747‑M1/1‑79 (MQ=255) ttttCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGTACCATaaaaaaaattgaccatcaaggcaaaatatcccgccccgttaaacgtattatttttcattaaaaatgcttacgtatga > 2:151193‑M1/1‑72 (MQ=255) tttggaTAAGGTCCGCAACGGACAGTCTTTACATAGGCAATATTT‑ATAGGTCTGTTACACTTATAAAAAAAAAAAAA‑‑‑‑AAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGc < 2:666203/144‑1 (MQ=255) tttCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGTACCATaaaaaaaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgca > 1:839986‑M1/1‑71 (MQ=255) tAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGTACCATaaaaaaaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttca > 1:468821‑M1/1‑66 (MQ=255) ctgTCCTCTACGGACGGTCTTTGTATAGGGAATATTT‑TCAGGTTTGTTACACTTATCCTACCATaaaaaaaattgaccttcaaggcaaaatatcccgccccggtagacgtattatttttcattaaaaatgcttaagtatgcatttcaat < 2:480523‑M1/148‑86 (MQ=255) aCTGACAGTCTTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGTACCATaaaaaaaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacga > 1:33432‑M1/1‑55 (MQ=255) cAGTCTTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGTACCATaaaaaaaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagtg < 2:942747‑M1/149‑100 (MQ=255) tCTTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGTACCATaaaaaaaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagtgcaa > 2:548966‑M1/1‑47 (MQ=255) cTTTTTATAGGGAATATTT‑ACAGGGTTGTTACACTTATCGTACCATAAAAA‑‑‑‑AAATTGACCATAAAGGGAAAATATCCCGCCCCGGTGGACGTATTATTTTTCATTAAGAATGCTTAAATATGCATTTCAAAGCACCACGAAAGaacaac > 1:91187/1‑149 (MQ=255) gggAATAATT‑ACAGGTTGGTTACACTTATCGTACCATaaaaaaaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtaagcatttcaatgcgccacgatagtgcaacaaacctggc < 1:617991‑M1/149‑113 (MQ=255) tttGTTACACTTATCGTACCATaaaaaaaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagtgcaacaaacctggcgtgcaccatgatgat > 1:244604‑M1/1‑22 (MQ=255) tGTTACACTTATCGTACCGTaaaaaaaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatcgtgcaacaaccctggcgtgcaccatgatgatca < 1:610806‑M1/149‑130 (MQ=255) tGTTACACTTATCGTACCATaaaaaaaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagtgcaacaaacctggcgtgcaccatgatgatca < 2:503299‑M1/149‑130 (MQ=255) ttACACTTATCGTACCATaaaaaaaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgctaaagtatgcatttcaatgcaccacgatagtgcaacaaacctggcgtgcaccatgatgatcatt > 1:208821‑M1/1‑18 (MQ=255) acTTATCGTACCATaaaaaaaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagggcaacaaacctggcgtgcaccgtgatgatcattccat > 1:410893‑M1/1‑14 (MQ=255) aTCGTACCATaaaaaaaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagtgcaacaaacctggcgtgcaccatgatgatcattccatcagg > 1:450644‑M1/1‑10 (MQ=255) | TTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT‑ACAGGTTTGTTACACTTATCGTGCCAT‑‑‑‑A‑‑‑‑AAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCAT > CP009273/2420189‑2420463 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |