New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 = 352785549 (1.040)4 (0.090) 4/274 5.2 8.1% intergenic (+56/+20) pck/envZ phosphoenolpyruvate carboxykinase/sensory histidine kinase in two‑component regulatory system with OmpR
?CP009273 = 3527861 46 (1.050)intergenic (+62/+14) pck/envZ phosphoenolpyruvate carboxykinase/sensory histidine kinase in two‑component regulatory system with OmpR
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CTGGCGAAACTGTTTATCGACAACTTCGATAAATACACCGACACCCCTGCGGGTGCCGCGCTGGTAGCGGCTGGTCCGAAACTGTAATGATTTGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTTTATTTACCCTTCTTTTGTCGTGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCA  >  CP009273/3527713‑3528002
                                                                                                                                              |                                                                                                                                                   
cTGGCTAAACTGTTTATCGACAACTTCGATAAATACACCGACACCCCTGCGGGTGCCGCGCTGGTAGCGGCTGGTCCGAAACTGTAATGATTTGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTc                                                                                                                                               <  2:738563/149‑1 (MQ=255)
 tGGCGAAACTGTTTATCGACAACTTCGATAAATACACCGACACCCCTGCGGGTGCCGCGCTGGTAGCGGCTGGTCCGAAACTGTAATGAATTGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCg                                                                                                                                              >  1:555992/1‑149 (MQ=255)
  ggCGAACCTGTTTATCGACAACTTCGATAAATACACCGACACCCCTGCGGGTGCCGCGCTGGTAGCGGCTGGTCCGAAACTGTAATGATTTGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGc                                                                                                                                             <  1:361014/149‑1 (MQ=255)
        aCTGTTTATCGACAACTTCGATAAATACACCGACACCCCTGCGGGTGCCGCGCTGGTAGCGGCTGGTCCGAAACTGTAATGATTTGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGcgaaggtgcctcccg                                                                                                                                       <  1:608528/149‑15 (MQ=255)
          tGTTTATCGACAACTTCGATAAATACACAGACACCCCTGCGGGTGCCGCGCTGGTAGCGGCTGGTTAGAAACTACAATGATTTGAAGCTGGAGAATATCCATCCAGTATCTTATAGAAAGCAAAACCGGAGGCACCTTCGCCTCCCGtt                                                                                                                                     >  2:340012/1‑149 (MQ=255)
            tctagtgACAACTTCGATAAGTACACCGACCCCCCTGCGGGTGCCGCGCTGGTAGCGGCTGGTCCGAAACTGTAATGATTTGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGttta                                                                                                                                   <  1:613552/143‑1 (MQ=255)
              tATCGACAACTTCGATAAATACACCGACACCCCTGCGGGTGCCGCGCTGGTAGCGGCTGGTCCGAAACTGTAATGATTTGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCACCCGtttatt                                                                                                                                 >  2:105236/1‑149 (MQ=255)
                 cGACAACTTCGATAAATACACCGACACCCCTGCGGGTGCCGCGCTGGTAGCGGCTGGTCCGAAACTGTAATGATTTGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTTTATTTAc                                                                                                                              >  2:764927/1‑149 (MQ=255)
                  gACAACTTCGATAAATACACCGACACCCCTGCGGGTGCCGCGCTGGTAGCGGCTGGTCCGAAACTGTAATGATTTGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTTTATTTAcc                                                                                                                             <  1:619230/149‑1 (MQ=255)
                   aCAACTTCGATAAATACACCGACACCCCTGCGGGTGCCGCGCTGGTAGCGGCTGGTCCGAAACTGTAATGATTTGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACCGGAGGCACCTTCGCCTCCCGTTTATTTAccc                                                                                                                            >  2:301683/1‑149 (MQ=255)
                        ttCGATAAATACACCGACACCCCTGCGGGTGCCGCGCTGGTAGCGGCTGGTCCGAAACTGTAATGATTTGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCTTTTATTTACCcttctt                                                                                                                       <  1:669933/149‑1 (MQ=255)
                            aTAAATACACCGACACCCCTGCGGGTGCCGCGCTGGTAGCGGCTGGTCCGAAACTGTAATGCTTTGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCAGTTTATTTACCCATCTTTTGt                                                                                                                   >  2:2474/1‑149 (MQ=255)
                                aTACACCGACACCCCTGCGGGTGCCGCGCTGGTAGCGGCTGGTCCGAAACTGTAATGATTTGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCCCCTTCGCCTCCCGTTCATTTACCCTTCTTTTGTCGTg                                                                                                               >  2:261107/1‑149 (MQ=255)
                                    acCGACACCCCTGCGGGTGCCGCGCTGGTAGCGGCTGGTCCGGAACTGTAATGATTTGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTATATTTACCCTTCTTTTGTCGTGCCCt                                                                                                           >  2:23286/1‑149 (MQ=255)
                                    acCGACACCCCTGCGGGGGCCGCGCTGGTAGCGGCTGGTCCGAAACTGTAATGATTTGAAGCGGGAGAATATCGAGCCAGGAGCTTATGGAAAGCAAAACGGGAGCCACCTCCGCCTCCCGTTTCTTTCCCCTCCCTTTGTCGTGCCCt                                                                                                           >  1:107088/1‑149 (MQ=255)
                                      cGACACCCCTGCGGGTGCCGCGCTGGTAGCGGCTGGTCCGAAACTGTAATGATTTGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTTTATTTACCCTTCTTTTGTCGTGCCCTgc                                                                                                         >  2:482098/1‑149 (MQ=255)
                                      cGACACCCCTGCGGGTGCCGCGCTGGTAGCGGCTGGTCCGAAACTGTAATGATTTGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTTTATTTACCCTTCTTTTGTCGTGCCCTgc                                                                                                         >  1:149775/1‑149 (MQ=255)
                                                    gTGCCGCGCTGGTAGCGGCTGGTCCGAAACTGTAATGATTTGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTTTATTTACCCTTCTTTTGTCGTGCCCTGCGCCCGCGTTACCgg                                                                                           <  1:471534/149‑1 (MQ=255)
                                                    gTGCCGCGCTGGTAGCGGCTGGTCCGAAACTGTAATGATTTGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTGTATTTACCCTCCTTTTGTCGTGCCCTGCGCCCCCGTTACCgg                                                                                           >  1:488289/1‑149 (MQ=255)
                                                      gCCGCGCTGGTAGCGGCTGGTCCGAAACTGTAATGATTTGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTTTATTTACCCTTCTTTTGTCGTGCCCTGCGCCCGCGTTACCGGCa                                                                                         <  2:393612/149‑1 (MQ=255)
                                                      gCCGCGCTGGTAGCGGCTGGTCCGAAACTGTAATGATTTGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTTTATTTACCCTTCTTTTGTCGTGCCCTGCGCCCGCGTTACCGGCa                                                                                         <  2:769592/149‑1 (MQ=255)
                                                        cgcgCTGGTAGCGGCTGGTCCGAAACTGTAATGATTTGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTTTATTTACCCTTCTTTTGTCGTGCCCTGCGCCCGCTTTACCGACACt                                                                                       >  2:454671/1‑149 (MQ=255)
                                                        cgcgCTGGTAGCGGCTGGTCCGAAACTGTAATGATTTGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTTTATTTACCCTTCTTTTGTCGTGCCCTGCGCCCGCGTTACCGGCACt                                                                                       >  2:650696/1‑149 (MQ=255)
                                                         gcgcTGGGAGCGGCTTGTCCGAAACTGTAATGATTTGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTTTATTTCCCCTTCTTTTGTCGTGCCCTGCGCCCGCGTTACCGGCACTg                                                                                      <  1:745124/149‑1 (MQ=255)
                                                           gcgggTAGCCGACGGCCCTAAACTGTAATGATTTGACGCTGGAGAATATCTATCCAGCAGCTTATAGAAATCAAATCGGTAGGTACCTTCGCCTCCCGTTTATTTACCCTTCTTTTGTCGTGCCCTGCGCCCGCGTTACCGGCACTGGc                                                                                    <  1:121309/146‑1 (MQ=255)
                                                             tGGTAGCGGCTGGTCCGAAACTGTAATGATTTGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTTTATTTACCCTTCTTTTGTCGTGCCCTGCGCCCGCGTTACCGGCACTGGCAg                                                                                  <  1:764927/149‑1 (MQ=255)
                                                                 aGCGGCTGGTCCGAAACTGTAATGATTTGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTTTATTTACCCTTCTTTTGTCGTGCCCTGCGCCCGCGTTACCGGCACTGGCAGcccg                                                                              >  2:423946/1‑147 (MQ=255)
                                                                    ggCTGGTCCGAAACTGTAATGATTTGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTTTATTTACCCTTCTTTTGTCGTGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGgcg                                                                           <  2:630736/149‑1 (MQ=255)
                                                                          tCCGAAACTGTAATGATTTGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTTTATTTACCCTTCTTTTGTCGTGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGCATg                                                                     >  1:543293/1‑149 (MQ=255)
                                                                             gAAACTGTAATGATTTGAAGCTGGAGAATATCTATCCAGTATCTTCTAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTTTATTTACCCTTCTTTTGTCGTGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGaa                                                                  >  2:730068/1‑149 (MQ=255)
                                                                              aaaCTGTAATGATTTGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTTTATTTACCCTTCTTTTGTCGTGCCCTCCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGaaa                                                                 <  2:555992/149‑1 (MQ=255)
                                                                                        gATTTGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTTTATTTACCCTTCAATTGCCTTGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGcccgccccg                                                       >  2:565168/1‑149 (MQ=255)
                                                                                          tttGAAGCTGGAGAATATCTATCCAGTATCTTGTAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTTTATTTACCCTTCTTTTGTCGTGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCcgct                                                     <  2:72269/149‑1 (MQ=255)
                                                                                          tttGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTTTATTTACCCTTCTTTTGTCGTGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCcgct                                                     <  2:627064/149‑1 (MQ=255)
                                                                                           ttGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGCGGAACCTTCGCCTCCCGTTTATTTACCCTTCTTTTGTCGTGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCcgctc                                                    <  2:135898/149‑1 (MQ=255)
                                                                                            tGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTTTATTTACCCTTCTTTTGTCGTGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCACGCGCGCATGGAAAGCCCGCCCcgctcg                                                   >  2:539180/1‑149 (MQ=255)
                                                                                               aGCTGGAGCATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTTTATTTACCCTTCTTTTGTCGTGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTg                                                <  2:254150/149‑1 (MQ=255)
                                                                                               aGCTGGAGACTATTTATTCAGTATCTTAAAGAAATCAAAACGGGGGGCAACTTCGCCGCCCGGTTATTTACCCTTCTTTTGTCGTGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTg                                                <  2:254053/149‑1 (MQ=255)
                                                                                                    gagaATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTTTATTTACCCTTCTTTTGTCGTGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGcc                                           <  2:694486/149‑1 (MQ=255)
                                                                                                     agaaattctatcCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTTTATTTACCCTTCTTTTGTCGTGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCa                                          <  1:211353/143‑1 (MQ=255)
                                                                                                          atctatcCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTTTATTTACCCTTCTTTTGTCGTGCCCTGCGCCCGCGTTACCGGCACGGGCAGCCAGACGCGAATGGAAAGCCCGCCCCGCGCGCTGGTGCCAAGCTc                                     >  1:68285/1‑149 (MQ=255)
                                                                                                            ctatcCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTTTATTTACCCTTCTTTTGTCGTGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCTCCGCTCGCTGGTGCCAAGCTCCa                                   <  1:666944/149‑1 (MQ=255)
                                                                                                            ctatcCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTTTATTTACCCTTCTTTTGTCGTGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCa                                   <  1:481298/149‑1 (MQ=255)
                                                                                                            ctatcCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTTTATTTACCCTTCTTTTGTCGTGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCa                                   <  1:666952/149‑1 (MQ=255)
                                                                                                                    tATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTGCCGTTTATATACACTTATATTGGGGTGCGCTGCGCCCGCGTTACCGGCACTGTCAGCCAGGCGGGAATGGACAGCCCGCCCCGCCCGCCGGTGCCAAGCTCCAGCATccgc                           >  1:583186/1‑147 (MQ=255)
                                                                                                                        ttATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTTTATTTACCCTTCTTTTGTCGTGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCACCGCTCGCTGGTGCCAAGCCCCAGCATCCCGTTAt                       >  2:593518/1‑149 (MQ=255)
                                                                                                                         tataGAAAGCAAAACGGGAGGCACCTTCGCCTCCTGTTTATTTACCTTTCTTTTGTTGTGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATg                      <  2:272923/149‑1 (MQ=255)
                                                                                                                           taGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTTTATTTACCCTTCTTTTGTCGTGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGt                    >  1:79323/1‑149 (MQ=255)
                                                                                                                                         ggAGGCACCTTCGCCTCCCGTTTATTTACCCTTCTTTTGTCGTGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGc      <  2:675655/149‑1 (MQ=255)
                                                                                                                                             gCACCTTCGCCTCCCGTTTATTTACCCTTCTTTTGTCGTGCCCTGCGCCCGCATTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGca  <  1:429016/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
CTGGCGAAACTGTTTATCGACAACTTCGATAAATACACCGACACCCCTGCGGGTGCCGCGCTGGTAGCGGCTGGTCCGAAACTGTAATGATTTGAAGCTGGAGAATATCTATCCAGTATCTTATAGAAAGCAAAACGGGAGGCACCTTCGCCTCCCGTTTATTTACCCTTCTTTTGTCGTGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCA  >  CP009273/3527713‑3528002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.