Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 4,048,586 A→T V18E (GTG→GAG)  glnG ← fused DNA‑binding response regulator in two‑component regulatory system with GlnL: response regulator/sigma54 interaction protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,048,5860AT96.8% 285.3 / ‑1.9 94V18E (GTG→GAG) glnGfused DNA‑binding response regulator in two‑component regulatory system with GlnL: response regulator/sigma54 interaction protein
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base T (52/39);  total (54/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGAT  >  CP009273/4048441‑4048729
                                                                                                                                                 |                                                                                                                                               
gAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTCCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCCAGCGCACGTTCAAGCcccc                                                                                                                                              >  2:1539727/1‑149 (MQ=255)
  gcagcGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCaa                                                                                                                                            >  2:1004396/1‑149 (MQ=255)
      cgcCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGa                                                                                                                                        <  2:752778/149‑1 (MQ=255)
      cgcCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGa                                                                                                                                        <  1:1569526/149‑1 (MQ=255)
       gcCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGAt                                                                                                                                       <  1:135681/149‑1 (MQ=255)
          aGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGa                                                                                                                                    <  1:359818/149‑1 (MQ=255)
          aGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCACGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCACCAGCGAGCGCACGTTCACGCTCCCACCGGATgaa                                                                                                                                    >  2:245911/1‑147 (MQ=255)
           gCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCTTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGaa                                                                                                                                   <  1:567068/149‑1 (MQ=255)
           gCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGaa                                                                                                                                   <  1:646784/149‑1 (MQ=255)
             ccGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGGACTTCTGCGCCGTTCTCAAACGTCGTACAGGGTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGATCt                                                                                                                                 >  2:1238685/1‑149 (MQ=255)
             ccGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGCTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACt                                                                                                                                 >  2:1249935/1‑149 (MQ=255)
               gTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTAt                                                                                                                               <  1:1571453/149‑1 (MQ=255)
                   aTTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTc                                                                                                                           >  2:221773/1‑149 (MQ=255)
                     tCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCAt                                                                                                                         <  1:5244/149‑1 (MQ=255)
                       ccGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCATCATCg                                                                                                                       >  1:1537018/1‑149 (MQ=255)
                       ccGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGACTCCAGCACTTCTGCGCCGTTCTCAAACGCCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTCCAAGCTCCCAACGGATGGAACTATCGTCATCg                                                                                                                       >  2:1211243/1‑149 (MQ=255)
                            aTACGGATACCTGAAAGCAGCACCTCCGGCGCTTCGCCCGCCGCCCTCACGATAACGTGTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTAc                                                                                                                  <  1:565230/149‑1 (MQ=255)
                                ggATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCCGCGCCTCCAGCACTTCTGCGCCGCTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAg                                                                                                              <  1:1362017/149‑1 (MQ=255)
                                ggATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAg                                                                                                              >  1:1810831/1‑149 (MQ=255)
                                 gATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGa                                                                                                             >  2:1833698/1‑149 (MQ=255)
                                 atcttCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGa                                                                                                             <  1:1109762/145‑1 (MQ=255)
                                     tCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGCTGGAACTATCGTCATCGACTACCCAGACTAt                                                                                                         >  1:103267/1‑149 (MQ=255)
                                     tCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGCCTAt                                                                                                         >  1:52847/1‑149 (MQ=255)
                                      cTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGAcagat                                                                                                        >  2:1010495/1‑145 (MQ=255)
                                       tGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATcc                                                                                                       >  1:1687896/1‑149 (MQ=255)
                                         aaaGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCt                                                                                                     >  1:103672/1‑149 (MQ=255)
                                           agcagcACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACCTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGCCTATCCCCCg                                                                                                   >  1:1575637/1‑149 (MQ=255)
                                             cagcaCATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGtt                                                                                                 <  1:74106/149‑1 (MQ=255)
                                             cagcaCATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGtt                                                                                                 <  1:593502/149‑1 (MQ=255)
                                              agcaCATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTg                                                                                                <  1:544013/149‑1 (MQ=255)
                                               gcaCATCCGCCGTTTTGCTCGCCAGCGCCGCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGc                                                                                               <  1:934384/149‑1 (MQ=255)
                                                   aTCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTGGCATaa                                                                                           >  1:1227075/1‑149 (MQ=255)
                                                    tCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATaaa                                                                                          >  1:1369041/1‑149 (MQ=255)
                                                     ccGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAAc                                                                                         <  1:1927049/149‑1 (MQ=255)
                                                       ggCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGt                                                                                       <  1:871051/149‑1 (MQ=255)
                                                         cGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCa                                                                                     <  1:1662696/149‑1 (MQ=255)
                                                           ttttGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACTTGCCTCAGCGAGCGCGCGTTCGAGCTCCGGACGGATGGAACTAGCGTGAACACCTACCCAGACTATCCCCCGTCGCATAAACGTCAcc                                                                                   >  2:161813/1‑149 (MQ=255)
                                                           ttttGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATACACGTCAcc                                                                                   >  2:134244/1‑149 (MQ=255)
                                                            tttGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGGTCGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCt                                                                                  >  2:520749/1‑149 (MQ=255)
                                                              tGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCttt                                                                                >  1:1152748/1‑149 (MQ=255)
                                                               gCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTa                                                                               <  1:1723562/149‑1 (MQ=255)
                                                               gCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTa                                                                               >  1:614358/1‑149 (MQ=255)
                                                                cTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTAt                                                                              >  1:11653/1‑149 (MQ=255)
                                                                cTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTAACCTTTAt                                                                              >  1:448116/1‑149 (MQ=255)
                                                                cTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGGCATCGACTACCCAGACTATCCCGCGTTGCATAAACGTCACCTTTAt                                                                              >  1:1836264/1‑149 (MQ=255)
                                                                     cAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCt                                                                         <  1:121442/149‑1 (MQ=255)
                                                                     cAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCt                                                                         <  1:242756/149‑1 (MQ=255)
                                                                     cAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCt                                                                         >  1:309332/1‑149 (MQ=255)
                                                                          ccgccAGCACTTCTGCGGCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAg                                                                    <  1:1845231/146‑1 (MQ=255)
                                                                          ccTCCCGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAg                                                                    <  1:978773/149‑1 (MQ=255)
                                                                           cTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAgg                                                                   >  1:1321757/1‑149 (MQ=255)
                                                                             ccAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCa                                                                 <  1:978556/149‑1 (MQ=255)
                                                                              cAGCACTTCTGCGCCTTGTGCTACCGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGTATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAg                                                                <  2:1678314/149‑1 (MQ=255)
                                                                              cAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAg                                                                >  1:262903/1‑149 (MQ=255)
                                                                               aGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAgg                                                               >  1:134577/1‑149 (MQ=255)
                                                                               aGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGGTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAgg                                                               >  2:1836088/1‑149 (MQ=255)
                                                                                ccacgccTGCGCCGTTCTCAAACCGCGTACATGTTAAACCTGCCCCCGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTCTCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGt                                                              <  1:1854843/143‑1 (MQ=255)
                                                                                    ttCTGCGCCTTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAAc                                                          <  1:1311161/149‑1 (MQ=255)
                                                                                    ttCTGCGCCGTTCTCAAACGTCGTACAGGTTAACCCTGCCCCAGCGCGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAAc                                                          <  1:379477/149‑1 (MQ=255)
                                                                                    ttCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAAc                                                          <  1:1970196/149‑1 (MQ=255)
                                                                                    ttCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCATCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAAc                                                          <  1:1946475/149‑1 (MQ=255)
                                                                                    ttCCGCGCCGTTCTCAAATGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAAc                                                          <  1:1311141/149‑1 (MQ=255)
                                                                                       tGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTCATTTCCTGATAGGCAGGTAAACCga                                                       >  1:1019767/1‑149 (MQ=255)
                                                                                            cGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACt                                                  <  1:1103437/149‑1 (MQ=255)
                                                                                                 tcAAACGTCGTACCGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTa                                             <  1:1756371/149‑1 (MQ=255)
                                                                                                   aaaCGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATg                                           >  2:1157946/1‑149 (MQ=255)
                                                                                                     aCGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGcc                                         <  1:1785540/149‑1 (MQ=255)
                                                                                                     aCGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAAATCGGTATGcc                                         >  2:1776522/1‑149 (MQ=255)
                                                                                                           tACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCCTAGACGTCCCCTTTATTTCCTGATAGGCAGGTAAACAGAGAACCCGGTATGCCCTGGcc                                   >  2:374894/1‑149 (MQ=255)
                                                                                                            aCAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCGCGGGCCa                                  >  2:71518/1‑149 (MQ=255)
                                                                                                            aCAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCa                                  >  2:1348668/1‑149 (MQ=255)
                                                                                                                gTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCCGGTAAACCGAGAACTCGGTAGGCGCTGGCCACCTg                              >  2:1658366/1‑149 (MQ=255)
                                                                                                                gTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGGCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTg                              >  1:255194/1‑149 (MQ=255)
                                                                                                                   aaaCCGGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTa                           <  1:242052/149‑1 (MQ=255)
                                                                                                                    aaCCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTAACGGCACCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTaa                          >  2:1844292/1‑149 (MQ=255)
                                                                                                                     aCCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCACCTGGTaaa                         >  1:484879/1‑149 (MQ=255)
                                                                                                                       cTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAAtt                       >  1:1636108/1‑149 (MQ=255)
                                                                                                                        tGCCCCAGCGAGCGCACCTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACTTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTc                      <  2:560951/149‑1 (MQ=255)
                                                                                                                            ccAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAAtt                  >  1:1522782/1‑149 (MQ=255)
                                                                                                                            ccAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAAtt                  >  2:1618772/1‑149 (MQ=255)
                                                                                                                            ccAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAAtt                  >  1:185928/1‑149 (MQ=255)
                                                                                                                               gcgagcgCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTg               >  1:639510/1‑149 (MQ=255)
                                                                                                                                cgagcgCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAACTTCAAttttac              >  1:485017/1‑147 (MQ=255)
                                                                                                                                cgagcgCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGc              >  1:1759758/1‑149 (MQ=255)
                                                                                                                                cgagcgCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGc              <  2:1681150/149‑1 (MQ=255)
                                                                                                                                   gcgcACGTTCAAGCTCCCAACGGATGGTACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGCATGCCCCGGCCACCTGGTAACTTCAATTTTGCCTg           >  2:1733645/1‑149 (MQ=255)
                                                                                                                                     gcACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCCCCTTTATTTCCTGCTAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTACATTCAATTTTGCCTaaa         >  2:27056/1‑149 (MQ=255)
                                                                                                                                     gcACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGCTAGGCAGGTAAACCGAGACCTCGGTATGCCCCGGCCAACTGGTAAATTCAATTTTGccacac         >  2:27029/1‑145 (MQ=255)
                                                                                                                                     gcACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGaa         >  1:1147206/1‑149 (MQ=255)
                                                                                                                                     gcACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACACGATATGCCCTGGCCAACCGGTAAATTCAATTTTGCCtaca         >  2:489697/1‑146 (MQ=255)
                                                                                                                                      cACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAAt        >  1:276118/1‑149 (MQ=255)
                                                                                                                                       aCGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAAATTTGCCTGAATg       >  1:370234/1‑149 (MQ=255)
                                                                                                                                         gTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCt     <  2:10053/149‑1 (MQ=255)
                                                                                                                                            cAAGCTCCCAACGGATGGAACCATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGat  <  1:519438/149‑1 (MQ=255)
                                                                                                                                            cAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGCCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGat  <  1:59304/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGAT  >  CP009273/4048441‑4048729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑