Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	4,050,841	G→T	P185Q (CCG→CAG)	glnA ←	glutamine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,050,841	0	G	T	99.0%	300.9 / NA	96	P185Q (CCG→CAG)	glnA	glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (51/44);  minor base A (1/0);  total (52/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

AGCTTTTTTGGTCATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACGTGGGAACCGGAGATAGATGATCCGAAACGGATGTCATC  >  CP009273/4050696‑4050986                                                                                                                                                   |                                                                                                                                                  aGCTTTTTTGGTCATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCtgtg                                                                                                                                                >  1:810880/1‑149 (MQ=255) aGCTTTTTTGGTCAGGGTACTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTTTTCCATCACCAGACACATTTCAGATCGAATATCCTGAGCCGAGTCTACCtgtg                                                                                                                                                <  1:1107635/149‑1 (MQ=255)       tttGGTCATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCg                                                                                                                                          >  1:1824086/1‑149 (MQ=255)         tGGCCAGGGTTTTGAAGCGGGTAGCCACCTCGTTCTGACCAGCAGTCGCTACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGAAACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCggg                                                                                                                                        <  1:580280/149‑1 (MQ=255)           gtaaTGGGATTGAGGGGGGTAGCCACTCCGTCCTGACCAGCAGGCGCGACTT‑CTGGGTGATGGGCTTCGACCACCAGACCCATGTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGCGCCGAGTCTACCTGTGGAACCGGGaa                                                                                                                                      <  2:706368/146‑1 (MQ=255)            tcctGGGATTGAAGCGGGCAGCCACTTCGTTCTGACCAGCAGTCGCTACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAg                                                                                                                                     <  1:336030/146‑1 (MQ=255)            tCATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCGGCAGTCGCTACTT‑CGTGGTGATGGGCTGCAACCACCAGACCCATCTGTTCCATCACCAAACACATTTCAGAACGAATGTCCTGCGCCGAGTCTCCCTGTCGAACCGGGAAg                                                                                                                                     >  2:419349/1‑149 (MQ=255)            tCATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACGT‑CGTGGTGATGGGCTTCCACCACCCGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATAGCCGGAGCCGGGCCTCCCTGTGGAACCGGGCAg                                                                                                                                     >  2:642269/1‑149 (MQ=255)             cATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTT‑CGTGTTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGt                                                                                                                                    >  1:1127437/1‑149 (MQ=255)             cATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGt                                                                                                                                    >  1:122417/1‑149 (MQ=255)              aTGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACAAATTTCAGAACGAATATCCTGGGCCGAGTCTACCTGTGGAACCGGGAAGTa                                                                                                                                   >  1:1869314/1‑149 (MQ=255)              aTGGTATTGAAGCGGGTAGCCACTTCGTACTGCGGAGGAGTCGCTACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTa                                                                                                                                   >  2:1313730/1‑149 (MQ=255)                ggTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTT‑CGTGGTGATGGGCTTCCACCACCAGACCCATCTGTTCAATCACAAGCCACATTTCCGAACGAATCTCCTGAGCAGAGTCTACCTGTGGAACCGGGAAGTAAc                                                                                                                                 <  1:924348/149‑1 (MQ=255)                ggTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAAc                                                                                                                                 <  2:976541/149‑1 (MQ=255)                 gTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAAcc                                                                                                                                <  2:1771369/149‑1 (MQ=255)                   aTTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGACCCGAGTCTACCTGTCGAACCGGGAAGTAACCGc                                                                                                                              >  2:674466/1‑149 (MQ=255)                     tGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCt                                                                                                                            >  2:897665/1‑149 (MQ=255)                       aaGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTT‑CGGGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCTAGTTTACCTGTGGAACCGGGAAGTAACCGCCttt                                                                                                                          <  2:944016/149‑1 (MQ=255)                          cGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGGGGAACCGGGAAGTAGCCGCCTTTCAc                                                                                                                       >  2:145417/1‑149 (MQ=255)                                 ccACTTCGTTCTGACCAGCAGTCGCTACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGa                                                                                                                <  2:1747235/149‑1 (MQ=255)                                 ccACTTCGTTCTGACCAGCAGTCGCTACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGa                                                                                                                >  2:894573/1‑149 (MQ=255)                                 ccACGTCGGTCTGACCAGCGGTCGCTACTT‑CGGGGTGGTGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGa                                                                                                                <  2:73546/149‑1 (MQ=255)                                  cACTTCGTTCTGACCAGCAGTCGCTACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCggcc                                                                                                               >  2:1144661/1‑147 (MQ=255)                                  cACTTCGTTCTGACCAGCAGTCGCTACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGAc                                                                                                               <  1:708577/149‑1 (MQ=255)                                  cACTTCGTTCTGACCAGCAGTCGCTACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGAc                                                                                                               <  1:1940519/149‑1 (MQ=255)                                  cACTTCGTTCTGACCAGCAGTCGCTACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGAc                                                                                                               >  2:822913/1‑149 (MQ=255)                                   aCTTCGTTCTGACCAGCAGTCGCTACTT‑CTTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATACCGAGCCGAGTCTACCTGTGGGACCGGGAAGTAACCGCCTTTCACTgccggccg                                                                                                              >  2:1008064/1‑149 (MQ=255)                                   aCTTCGTTCTGACCAGCAGTCGCTACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACg                                                                                                              >  2:540805/1‑149 (MQ=255)                                     ttCGTTCTGACCAGCAGTCGCTACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCAGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGt                                                                                                            >  2:940449/1‑149 (MQ=255)                                        gTTCTGACCAGCAGTCGCTACTT‑CGTGGTGATGGGCTTCCACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTTAc                                                                                                         >  2:1811705/1‑149 (MQ=255)                                           cTGACCAGCAGTCGCTACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCtt                                                                                                      <  2:74221/149‑1 (MQ=255)                                             gACCAGCAGTCGCTACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGCTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGGAGTAACCGCCTTTCACTGCCGGACGGTGACCGTTg                                                                                                    >  2:1596188/1‑149 (MQ=255)                                             gACCAGCAGCCGCTACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCAACACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTg                                                                                                    <  1:1659875/149‑1 (MQ=255)                                              aCCAGCAGCCGCTATTTGGGTGGT‑ATTGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCGGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGt                                                                                                   <  1:177/149‑1 (MQ=255)                                               cctgcggTCGCTACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGtt                                                                                                  <  2:151210/143‑1 (MQ=255)                                                cagcagTCGCCACTG‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTa                                                                                                 <  1:390477/149‑1 (MQ=255)                                                  gcagTCGCTACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCCCTCCACTCCCCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTacc                                                                                               <  1:58058/149‑1 (MQ=255)                                                     gTCGCTACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTaccacc                                                                                            <  2:313016/149‑1 (MQ=255)                                                      tCGCTACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACAAGACACATTTCAGAACGAATGTCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGCCCTTTGTTACCACCt                                                                                           >  1:344036/1‑149 (MQ=255)                                                      tCGCTACTG‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCt                                                                                           <  1:1341146/149‑1 (MQ=255)                                                         cTACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCAGCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCg                                                                                        <  2:548108/149‑1 (MQ=255)                                                          tACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGt                                                                                       >  2:493271/1‑149 (MQ=255)                                                          tACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGt                                                                                       >  1:1328797/1‑149 (MQ=255)                                                              t‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTTGGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTg                                                                                   <  1:566227/149‑1 (MQ=255)                                                              t‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTAAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTg                                                                                   >  1:1674885/1‑149 (MQ=255)                                                               gcGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTg                                                                                   <  1:740395/148‑1 (MQ=255)                                                                cGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTCTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGGATTgg                                                                                  >  2:1167575/1‑149 (MQ=255)                                                                  tggtggtggGCTTCAACCTCCAGACCCCTCTGTTGCTCCCCCAGTCACATGTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGACCCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGt                                                                                <  1:1050137/149‑1 (MQ=255)                                                                  tggtgATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGt                                                                                <  2:737945/149‑1 (MQ=255)                                                                   ggtgATGGGCTTCAACCACCCGACCCATCTGTTCCGTCACCAGACACATTTCAGAACGAATTTCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTg                                                                               <  2:1453627/149‑1 (MQ=255)                                                                    gtgtTGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATATCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTgg                                                                              <  2:1117871/149‑1 (MQ=255)                                                                    gtgATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTgg                                                                              >  2:1514131/1‑149 (MQ=255)                                                                    gtgATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATAACCTGAGCCGAGACTAACTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGAACGTTGTTACCACCTTCGGAgtgggtgg                                                                              >  1:67070/1‑149 (MQ=255)                                                                     tgctGGGCGTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACGGCCGGGCGGTGACCGTTGTTGCCCCCTTCGGCTTGGGGgga                                                                             >  2:1614131/4‑149 (MQ=255)                                                                      gATGGGCTTCAACCACCAGACCCTGCGCTTCCTTCACCAGACACATTACCGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTggag                                                                            <  1:270518/149‑1 (MQ=255)                                                                       aTGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAAAATCATAAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGACCGGTGACCTTGGTTACACCCTTAGTATTGGGTggagg                                                                           >  1:811351/1‑149 (MQ=255)                                                                         gggCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACAAATATCCTGCGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGGCGGTGACCTTTGTTACCACCTTCGTCTTGGGTggcgggg                                                                         >  1:1651429/1‑147 (MQ=255)                                                                          ggCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTCCGTATTGGGTGGCGGAGt                                                                        >  2:1660417/1‑149 (MQ=255)                                                                           gCTTCAACCACCAGACCCATTTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGtt                                                                       <  1:1809470/149‑1 (MQ=255)                                                                            ctccAACCCCCAGACCCATCTGGTCCAGCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCGTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTc                                                                      <  1:926215/146‑1 (MQ=255)                                                                            cTTCAACCACCAGACCCATCTGTTCCATCACCTGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATCGGGTGGAGGAGTTc                                                                      >  2:1326354/1‑149 (MQ=255)                                                                             ttCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGACTTGGGCGGAGGAGTTcc                                                                     >  1:401586/1‑149 (MQ=255)                                                                                 accaccAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATgc                                                                 <  1:1816124/149‑1 (MQ=255)                                                                                   caccaGACCCATCTGTTCCATCACCAGACACATTTCAAAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGGGGAGTTCCATgggc                                                               >  1:1178522/1‑149 (MQ=255)                                                                                      caGACCCATCTGTTCCAGCACCAGACACATGTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTCTTGGGTGGAGGAGGTCCATGCGCCtt                                                            >  2:1078433/1‑149 (MQ=255)                                                                                         aCCCATCCGTTCCATCACCAGACACCTTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGa                                                         <  1:85876/149‑1 (MQ=255)                                                                                          cccATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGat                                                        <  1:592047/149‑1 (MQ=255)                                                                                             aTCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATAtc                                                     >  2:529679/1‑149 (MQ=255)                                                                                               cTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATAtcgt                                                   >  1:860198/1‑149 (MQ=255)                                                                                               cTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATAtcgt                                                   <  1:1337706/149‑1 (MQ=255)                                                                                                ggtgccAGCACCAGACACATTGCAGAACGAATATCCTGGGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATAtcgtc                                                  <  1:65571/145‑1 (MQ=255)                                                                                                 gTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTATCCCTGTAGAACCAGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGAATAGGGAGGAGGAGTTCCATGCGCCTTCGGTAtcgtcg                                                 >  1:396990/1‑149 (MQ=255)                                                                                                  ttCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGa                                                <  1:906123/149‑1 (MQ=255)                                                                                                   tCCATCACCAGCCACATTTCAGCACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAGGTAACCGCCTTGCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGAt                                               >  2:446745/1‑149 (MQ=255)                                                                                                    ctaTCACCAGACATATTTCAGAACGTATATCCTGGGCCGGGTCTACCTGTGGATCCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATa                                              <  2:61325/147‑1 (MQ=255)                                                                                                    ccATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATa                                              <  2:863451/149‑1 (MQ=255)                                                                                                    ccATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATa                                              >  2:1474423/1‑149 (MQ=255)                                                                                                      aTCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGc                                            >  2:1629320/1‑149 (MQ=255)                                                                                                      aTCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGGTCCATGCGCCTTCGATATCGTCGATAGc                                            >  2:1112326/1‑149 (MQ=255)                                                                                                        cACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACAGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATCGCGTCGATAGCac                                          >  1:766200/1‑148 (MQ=255)                                                                                                         aCCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGACTTCGATATCGTCGATAGCAAc                                         >  1:865300/1‑149 (MQ=255)                                                                                                         aCCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGGGACCTTTGTTACCACCTTCGTATTGGGTGAAGGAGTTCCATGCACCTTCGATATCGTCGTTAGCAAc                                         >  2:82792/1‑149 (MQ=255)                                                                                                            aGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGCAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACggg                                      >  1:1188072/1‑147 (MQ=255)                                                                                                                acaTTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACGTGGGaa                                  >  2:194892/1‑149 (MQ=255)                                                                                                                  aTTTCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTCCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGAAACCGTCGATAGCAACGTGGGAAcc                                >  1:1943759/1‑149 (MQ=255)                                                                                                                   tttCAGAACGAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTGGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGAAAGCCACGGGGGGACCg                               >  2:1739280/1‑149 (MQ=255)                                                                                                                    ttCAGCACGAATAGCCTGAGCCGGCGCTACCTGTGGACCCGGGAAGTAACCCCCTTTCACTGCCGTACGGTGACCTTTTTTCCCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACGTGGGAACCgg                              <  2:1566647/149‑1 (MQ=255)                                                                                                                           cGAATATCCGGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACGTGGGAACCGGagataga                       <  1:1230012/149‑1 (MQ=255)                                                                                                                            gAATATCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACGTGGGAACCGGggctagat                      >  1:842598/1‑149 (MQ=255)                                                                                                                              atatCCTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATCGCAACGTGGGAACCGGAGATAgatga                    >  2:247209/1‑149 (MQ=255)                                                                                                                                   cTGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACGTGGGAACCGGAGATAGATGATCCGa               <  2:399286/149‑1 (MQ=255)                                                                                                                                    tGAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTATCGTCGCTTTCGATATCGTCGATAGCAACGAGGGAACGGGAGCAAAATGAGCCGaa              >  1:467989/1‑149 (MQ=255)                                                                                                                                    tGAGCCGAGTCTACCTGTGGAACCGAGAAGCAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGAAAGCAACGTGGGAACCGGAGATAGGGGATCCGaa              >  1:1040765/1‑149 (MQ=255)                                                                                                                                     gAGCCGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACGTGGGAACCGGAGATAGATGATCCGaaa             <  2:781785/149‑1 (MQ=255)                                                                                                                                        ccGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACGTGGGAACCGGAGATAGATGATCCGAAACgg          <  1:1274979/149‑1 (MQ=255)                                                                                                                                        ccGAGTCTACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGAAATCGTCGATAGCAACGTGGGAAGCGGGGATAGATGATCCGGAACgg          >  1:1604314/1‑149 (MQ=255)                                                                                                                                               tACCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCAGGACGGTGATCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACGTGGGAACCGGAGATAGATGATCCGAAACGGATGTCAt   <  1:1549128/149‑1 (MQ=255)                                                                                                                                                aCCTGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACGTGGGAACCGGAGATAGATGATCCGAAACGGATGTCATc  <  1:1238098/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  AGCTTTTTTGGTCATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTT‑CGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACGTGGGAACCGGAGATAGATGATCCGAAACGGATGTCATC  >  CP009273/4050696‑4050986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑