Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 4,133,470 C→T A338V (GCG→GTG)  frwC → putative enzyme IIC component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,133,4700CT94.9% 274.6 / NA 101A338V (GCG→GTG) frwCputative enzyme IIC component of PTS
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (51/42);  minor base G (3/2);  total (56/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

CGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCA  >  CP009273/4133326‑4133614
                                                                                                                                                |                                                                                                                                                
cggcgCTGGTCGGTGCGCAGTGCGATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCCGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGt                                                                                                                                              >  1:1314887/1‑149 (MQ=255)
    gcTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTaaaa                                                                                                                                          >  1:1552235/1‑149 (MQ=255)
        gTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATgg                                                                                                                                      <  1:1650510/149‑1 (MQ=255)
          cGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGt                                                                                                                                    <  2:1349375/149‑1 (MQ=255)
            gTGCGCAGTGCTATGCAGGCTGGGGTGGCCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCGGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCt                                                                                                                                  <  1:975979/149‑1 (MQ=255)
             tGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATAGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCtt                                                                                                                                 >  1:1283038/1‑149 (MQ=255)
             tGCGCAGTGCTATGCAGGCGGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCAGTCGGAGCAGTGGTGTCGGCTGTTTGTGTGAACGTGCGGAAAAGTCTGGTGCGTAAAAATGGGGCtt                                                                                                                                 >  1:1790818/1‑149 (MQ=255)
              gcgcAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTc                                                                                                                                <  2:1699788/149‑1 (MQ=255)
                gcAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGGAAAAATGGGGCTTCGa                                                                                                                              >  1:409139/1‑149 (MQ=255)
                gcAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAATGGCTGTCGTAGCAGTGGTGACGGCTGTTGTTGTGAACTTGCGGAAATGGCTTTGGCGGAAATATGGGtttggga                                                                                                                              >  1:918442/1‑143 (MQ=255)
                gcAGTGCTATGCAGGCTGGGGTGATCTGCTTGTGATGCCGGTGGTTGAAGGCAGGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGGACGTGCTGAAAAGTCTGGTGCGTAATAATGGGTCTTCGa                                                                                                                              >  1:1109005/1‑149 (MQ=255)
                 cAGTGCTATGCAGGCTGGGGTGGTCTGGTTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAACTCTGGTGCGTAAAAATGGGTCTTCGAc                                                                                                                             >  1:1398815/1‑149 (MQ=255)
                  aGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACt                                                                                                                            >  1:1997844/1‑149 (MQ=255)
                    tGCTATGCAGGCTGGGGTGCGCTGATTGTGCTGCCGGTGGTCGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTAGGAGCAGTGGTTACGGCGGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACtga                                                                                                                          <  2:398077/149‑1 (MQ=255)
                      cTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGGGCGTAAAAATGGGTCTTCGACtgatg                                                                                                                        >  1:665326/1‑149 (MQ=255)
                       tATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGGGCGTAAAAATGGGTCTTCGACtgatga                                                                                                                       >  1:833499/1‑149 (MQ=255)
                        aTGCAGGCTGGGTTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGaa                                                                                                                      <  1:136481/149‑1 (MQ=255)
                          gCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGAGGaaaa                                                                                                                    >  2:1510215/1‑149 (MQ=255)
                            aGGCTGGGGTGGTGTGAGTGGGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACTGCTGGTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAaag                                                                                                                  <  1:386552/149‑1 (MQ=255)
                                tGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGCCTGATGAAAAagaaga                                                                                                              >  2:710405/1‑149 (MQ=255)
                                tGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAGAAagaaga                                                                                                              >  2:1366390/1‑149 (MQ=255)
                                      ggtCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCt                                                                                                        >  1:1331053/1‑149 (MQ=255)
                                       gtCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACGGATGAAAAAGAAGGCGACCtg                                                                                                       >  2:58796/1‑149 (MQ=255)
                                        tCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCtgg                                                                                                      >  1:853161/1‑149 (MQ=255)
                                           gATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCtggatt                                                                                                   >  2:1857422/1‑149 (MQ=255)
                                            aTTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCgggagtt                                                                                                  >  1:1077944/1‑149 (MQ=255)
                                             ttGTGCGGGCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCtggatttg                                                                                                 <  2:930223/149‑1 (MQ=255)
                                               gtgCTGCCGGTGGTTGAAGGCAAGCGTGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGGGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCtggatttgga                                                                                               <  1:524258/149‑1 (MQ=255)
                                               gggctgcCGGTGGTTGAAGGCAAGGTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGCCGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCtggatttgga                                                                                               <  2:1343323/147‑1 (MQ=255)
                                                   ggcCGGGGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGGCGGAGCAGTGGTGGCGGGTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTACAAATGGGTCTGCGACTGATGAAAAAGAAGACGACCTGGATTTGGAtttt                                                                                           <  1:1368228/148‑1 (MQ=255)
                                                    gcCGGTGGTTGAAGGCAAGCTTGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTg                                                                                          <  1:752276/149‑1 (MQ=255)
                                                      cGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGaa                                                                                        >  1:1092878/1‑149 (MQ=255)
                                                       ggtggtTGACGGCACGTTGAGAAAAAAAGCAGAATTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCATCGACTGACGAAAAAGACGACGACCTGGATTTGGCTTTTGaaa                                                                                       >  2:142493/1‑149 (MQ=255)
                                                       ggtggtTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGTCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGaaa                                                                                       >  1:891937/1‑149 (MQ=255)
                                                       ggtggtTGAAGGCAAGCTGGGTTATATCATATCATTGGCTGTTGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGaaa                                                                                       <  2:1687659/149‑1 (MQ=255)
                                                          ggtTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAaatta                                                                                    <  1:425852/149‑1 (MQ=255)
                                                               aaGGGAAGCTGGGTTATAGCGCAGCAGTGGCTGTCGGAGCAGTGGTGGCGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAaattaattaa                                                                               <  1:390679/149‑1 (MQ=255)
                                                               aaGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGAATTTGAacttaattaa                                                                               >  1:1614683/1‑149 (MQ=255)
                                                                aGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAaattaattaat                                                                              >  2:97828/1‑149 (MQ=255)
                                                                 ggCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGAGTTGGATGTTGAaattaattaatt                                                                             >  2:570393/1‑149 (MQ=255)
                                                                 ggCAAGCTGGGTTATATCGCAGCAGAGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGGCTGGTGCGTAAAAATGGGTCGTCGACTGATGAAAAAGAAGACGACCTGGACCTGGAGTTTGAaagtaattaatt                                                                             >  2:715545/1‑149 (MQ=255)
                                                                  gCAAGTTGGGTTATATCGCAGCAGGGGATGTCGGATCAGTTGTTACGACTGTTAGAGTGAACGTGATCAAAAAACTGAAGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTg                                                                            <  1:134224/149‑1 (MQ=255)
                                                                    aaGCTGGGTTATGTCGCCGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAg                                                                          <  1:285828/149‑1 (MQ=255)
                                                                    aaGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAg                                                                          <  1:1102273/149‑1 (MQ=255)
                                                                    aaGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGGTTGTGTGAACGTGCTGAAAAGTCTGGTGCGCAAAAATGGGCATGCGACAGATGAAAAAGAAGACGCCCTGGATTTGGACTTTGAAATCAATTACTTGAg                                                                          >  2:26497/1‑149 (MQ=255)
                                                                     aGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATATGGATTTTGAAATTAATTAATTGAgg                                                                         >  2:1682074/1‑149 (MQ=255)
                                                                      gCTGGGTTAGATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGa                                                                        <  2:1158257/149‑1 (MQ=255)
                                                                          ggTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGa                                                                    >  1:1340855/1‑149 (MQ=255)
                                                                          ggTGATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGa                                                                    <  2:376332/149‑1 (MQ=255)
                                                                            ttATATCGGAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGc                                                                  >  1:1465109/1‑149 (MQ=255)
                                                                            ttATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGc                                                                  <  2:1644928/149‑1 (MQ=255)
                                                                            atataTCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGc                                                                  >  2:881039/2‑149 (MQ=255)
                                                                               tatCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCAt                                                               >  1:569461/1‑149 (MQ=255)
                                                                                atCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGGGTAAAAATGGGACTTCGACTGATGAAAAAGGAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCAATg                                                              >  2:695576/1‑149 (MQ=255)
                                                                                 tCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGa                                                             <  1:1180754/149‑1 (MQ=255)
                                                                                  cGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGAc                                                            <  2:1661705/149‑1 (MQ=255)
                                                                                      gcagTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGaaa                                                        <  1:615163/149‑1 (MQ=255)
                                                                                       cagTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGGGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGaaaa                                                       <  1:600916/149‑1 (MQ=255)
                                                                                        agTGGGTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTATGGTGAGTAAAACTGGGTCTTCGATTGATGAAAAAGAAGACGACCTGGATCTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAat                                                      <  2:1111014/149‑1 (MQ=255)
                                                                                             gtGTCGGGGCAGTGGTGACGGGTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTg                                                 <  1:630292/148‑1 (MQ=255)
                                                                                             cTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTg                                                 <  2:1286009/149‑1 (MQ=255)
                                                                                             cTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTg                                                 <  1:1476913/149‑1 (MQ=255)
                                                                                              tGTCGGAGCAGTGGTGACGGCTGTTTGTGTGCTCGATTTAAAAAGGTTAGTTCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTCATTGAGGAACGCGCCATGACGAAAATTATTGc                                                <  2:834143/149‑1 (MQ=255)
                                                                                               gTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCa                                               <  1:42808/149‑1 (MQ=255)
                                                                                                tCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAg                                              >  1:337495/1‑149 (MQ=255)
                                                                                                  ggAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTa                                            >  2:132596/1‑149 (MQ=255)
                                                                                                   gAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTaa                                           >  1:1158173/1‑149 (MQ=255)
                                                                                                    aGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGCTTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGAAGCCCATTATTGCAGTAAc                                          <  2:1364934/149‑1 (MQ=255)
                                                                                                       ggtggtgACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGc                                       <  2:1434126/148‑1 (MQ=255)
                                                                                                       aGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGc                                       >  1:511022/1‑149 (MQ=255)
                                                                                                        gtggtgACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCa                                      >  1:773693/1‑149 (MQ=255)
                                                                                                         tggtgACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCAt                                     >  2:149391/1‑149 (MQ=255)
                                                                                                         tggtgACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCAt                                     >  1:1642996/1‑149 (MQ=255)
                                                                                                              aCGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGAATGATGAAAAAGAAGACGACCTGGATTTGGAGTTTGAGCATACTTAATTATGGAACGGGCCATGACGAAAATTATTGCAGTAACCGCATGTCCt                                >  1:660525/1‑149 (MQ=255)
                                                                                                               cGGCTGTTTGTGTGCACGTGCTGAAAAGGCTGGTGCGAAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCtt                               <  1:518574/149‑1 (MQ=255)
                                                                                                               cGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGAGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCtt                               >  2:618810/1‑149 (MQ=255)
                                                                                                                 gCTGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGACCCATGACGAAAATTATTGCAGTAACCGTATGTCCTTCa                             <  2:890930/149‑1 (MQ=255)
                                                                                                                 gCTGTGTGGGTGCGCGGGCTGAAGAGTCGGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATCTTGAAATTAATTAAGTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCa                             <  2:325553/149‑1 (MQ=255)
                                                                                                                  cTGTTTGGGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGATCAAAAGTATTGCAGTAACCGCATGTCCTTCAg                            <  1:1481458/149‑1 (MQ=255)
                                                                                                                   tGTTTGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTGATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAgg                           >  1:1658369/1‑149 (MQ=255)
                                                                                                                     tttGTGTGAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGGCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGAATGTACTTCAgggg                         >  1:1245423/1‑147 (MQ=255)
                                                                                                                     tttGGGTGAACGTGCTGAAAAGTCTGGGGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGgtg                         <  1:1688428/149‑1 (MQ=255)
                                                                                                                        gtgggAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTg                      <  2:1860832/149‑1 (MQ=255)
                                                                                                                         tgtgAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGc                     >  1:1838452/1‑149 (MQ=255)
                                                                                                                           tgAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGccc                   <  2:775540/149‑1 (MQ=255)
                                                                                                                           tgAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGccc                   >  1:192187/1‑149 (MQ=255)
                                                                                                                            gAACGTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGGGTTGCCCa                  >  2:1815998/1‑149 (MQ=255)
                                                                                                                               cGTGGTGAAAAGCCTGGGGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAAGTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATAc               <  1:139244/149‑1 (MQ=255)
                                                                                                                                gTGCTGAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATAcc              >  1:1677126/1‑149 (MQ=255)
                                                                                                                                     gAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCtcaat         >  2:757659/1‑145 (MQ=255)
                                                                                                                                     gAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTatat         <  2:1520693/149‑1 (MQ=255)
                                                                                                                                     gAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTatat         <  2:1220829/149‑1 (MQ=255)
                                                                                                                                     gAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTatat         >  2:869868/1‑149 (MQ=255)
                                                                                                                                     gAAAAGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTGTGAAATTAATTAATTGAGGAACGAGCCACGACGAAAATCATTGCTGTAACCGCATGTCCTTCAGGCGTTGCCCATACCtctct         >  2:1579325/1‑145 (MQ=255)
                                                                                                                                     gAAAAGTCTGGTGCGTAAAAATGGGTCTGCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTgtat         >  1:1310291/1‑149 (MQ=255)
                                                                                                                                      aaaaGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATg        <  1:1632232/149‑1 (MQ=255)
                                                                                                                                        aaGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCTCATACCTATATGgc      >  2:57760/1‑149 (MQ=255)
                                                                                                                                        aaGTCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGgc      >  1:1509707/1‑149 (MQ=255)
                                                                                                                                           tCTGGTGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGgcagc   >  2:691815/1‑149 (MQ=255)
                                                                                                                                            cTGGTGCGTAAAGATGGGTCTTCGACTGATGAAAAAGACGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAATGAGCCATGACGAAAATTATTGCAGTAGCCGCATGTCCTTCAGGTGTTGCCCATACCTATATGgcagca  <  2:735868/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCA  >  CP009273/4133326‑4133614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: