Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	3,219,839	C→G	R83G (CGT→GGT)	ygjI →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,219,839	0	C	G	100.0%	211.3 / NA	68	R83G (CGT→GGT)	ygjI	putative transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (40/28);  total (40/28)

GCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATT  >  CP009273/3219694‑3219979                                                                                                                                                  |                                                                                                                                             gCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGtt                                                                                                                                           >  2:358881/1‑149 (MQ=255) gCTTCGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAAAAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGtt                                                                                                                                           <  1:192563/149‑1 (MQ=255)   ttGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTgg                                                                                                                                         >  1:679318/1‑149 (MQ=255)   gtcgACTGGCCCCGGCACCGATGTTTTTCCTCGCGGCGGTTTTTTATTTTATTCCCTTCGGTCTGATCACGGCAGAATTTTTATAGATATAGGTATACTCAGTAGCGGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTgg                                                                                                                                         <  1:1097138/146‑1 (MQ=255)             cTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATttatta                                                                                                                               <  2:1036142/149‑1 (MQ=255)                ggCACCGATGTTTTTCCTCGCGACGATTTTTTATGTTATTCCCTTCTGTCTGATCATCGCAGAATTTTTTTCGTTAAATAAAAACTCAGGAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTGGCGCATTTATTGCTg                                                                                                                            >  1:998677/1‑149 (MQ=255)                     cGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCACAGAATTTGTTTCGTTCAATAAAAACTCAGAAGCCGGTGTCTAAGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCACttattatagaccat                                                                                                                       >  1:221694/1‑141 (MQ=255)                     cGATGTTCTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCtat                                                                                                                       <  2:353446/149‑1 (MQ=255)                           ttttCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTAc                                                                                                                 <  2:702340/149‑1 (MQ=255)                                cTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGGGGGTAAGAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGtt                                                                                                            >  1:125348/1‑149 (MQ=255)                                  cgcgGCGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGATTTTGAGTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCg                                                                                                          <  2:441623/149‑1 (MQ=255)                                  cgcgACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGttag                                                                                                          >  1:493477/1‑147 (MQ=255)                                    cgacgaTTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTAGACGGAGGTTGGGCATTTATCACTGCCGAGACCTACTGGTACgca                                                                                                        >  1:1076608/1‑147 (MQ=255)                                         aTTTTTTATTTTAGTCCCTTCTTTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCt                                                                                                   <  1:1005970/149‑1 (MQ=255)                                            ttttattttaTTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGtt                                                                                                <  2:222687/149‑1 (MQ=255)                                             tttattttatTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTc                                                                                               >  1:1094788/1‑149 (MQ=255)                                                attttagtCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCttt                                                                                            <  2:1127222/149‑1 (MQ=255)                                                    tattCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGGTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCa                                                                                        >  1:900269/1‑149 (MQ=255)                                                       tCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGAGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGGAAACCTGTTCTTTTTCCCCt                                                                                     >  1:1085904/1‑149 (MQ=255)                                                         ccTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCa                                                                                   <  1:824548/149‑1 (MQ=255)                                                           ttCTGTCTGATCATCGCAGAATTTGTGTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACt                                                                                 <  1:676616/149‑1 (MQ=255)                                                               gtctgATCATCGCAGAATTTGTTTCGTTAACTAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTg                                                                             <  1:349021/149‑1 (MQ=255)                                                                 ctgagtatcGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGcc                                                                           <  2:577752/143‑1 (MQ=255)                                                                    atcatcGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCCGCTCACTGTTGCcgcg                                                                        >  2:120292/1‑149 (MQ=255)                                                                        tcgcgaaaTTTTTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGGGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGtt                                                                    <  1:4378/143‑1 (MQ=255)                                                                        tcGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTAGGTAAAAAGGGCGCAATGCGGAGGTTGGGCATCTATTACTGCCTATACCTACTGGTTCGGAAACCTGCTCTTTTTCACCTCCCTGGTGCCGCGCGtt                                                                    >  1:810636/1‑149 (MQ=255)                                                                          gCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTAt                                                                  >  2:81190/1‑149 (MQ=255)                                                                           cAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTAtt                                                                 >  2:185220/1‑149 (MQ=255)                                                                            aGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTCTTg                                                                >  1:308781/1‑149 (MQ=255)                                                                              aaTTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCGGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCt                                                              >  1:164402/1‑149 (MQ=255)                                                                                tttGTTTCGTTAGACAACATCTCAGAAGCCGGTGTCTACGCGTGTGTAAAAAGTCCGCTGGGCGCAGGTGGGTCATTTATGTCTGGGTTGAGCTACTGGTTCGTAAACCTGTTCTCTTTCTCCTCACTGTTGCCGCGCGTTATTGCTTa                                                            <  2:221694/149‑1 (MQ=255)                                                                                   gTTTGGTTAAATAAAAACTCGGAAGCCGGTGTCTACGCGTGTGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGc                                                         <  1:337638/149‑1 (MQ=255)                                                                                   gTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGc                                                         <  2:349328/149‑1 (MQ=255)                                                                                    tttCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCt                                                        >  2:379444/1‑149 (MQ=255)                                                                                        gTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGt                                                    >  2:54468/1‑149 (MQ=255)                                                                                        gTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCGCACGGTTGCCGCGCGTTATTGCTTATGCTTCGt                                                    <  2:903255/149‑1 (MQ=255)                                                                                         ttAAATAAAAACCCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTa                                                   <  1:380660/149‑1 (MQ=255)                                                                                           aaataaaATCTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATg                                                 <  1:358881/149‑1 (MQ=255)                                                                                            aataaaAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGc                                                >  2:164683/1‑149 (MQ=255)                                                                                            aataaaAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGc                                                >  1:1019528/1‑149 (MQ=255)                                                                                            aataaaAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCGTATTCTTCGTATGc                                                >  2:5129/1‑149 (MQ=255)                                                                                              taaaAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATGCCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCCCTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCt                                              >  2:236270/1‑149 (MQ=255)                                                                                               aaaaaCTCAGAAGCCGGTGTCTACGCGTGGGAAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGACCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTCTACTTCGTATGCCtt                                             >  2:555527/1‑149 (MQ=255)                                                                                                aaaaCTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGGGCTTATTACTGCCTATACCTACTGGTTCGTAAACCTGGTCTTTTTCACCTCAGTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGGCTTc                                            >  2:1013264/1‑149 (MQ=255)                                                                                                aaaaCTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGGGGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTc                                            >  1:755892/1‑149 (MQ=255)                                                                                                 aaaCTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTcc                                           >  2:652769/1‑149 (MQ=255)                                                                                                   aCTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCCCCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTc                                         >  1:548551/1‑149 (MQ=255)                                                                                                   aCTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTc                                         <  2:1094788/149‑1 (MQ=255)                                                                                                     tCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATGTATTACTGCCTATACCTACTGGTTCGGAAACCTGTTCTTTTTCACCTCACTTTTGCCGCCGGTTGTTCGTATTGCCTCGTATACCTTctcctt                                       >  1:226511/1‑144 (MQ=255)                                                                                                        gAAGCCGGGTTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATa                                    <  1:678919/149‑1 (MQ=255)                                                                                                         aaGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGGTTATGCTTCGTATGCGTTCCTCGGATAc                                   >  1:969444/1‑149 (MQ=255)                                                                                                             cGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACTTCACTGTTGCCGCGAGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAat                               >  2:1042751/1‑149 (MQ=255)                                                                                                               gtgtCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCATCTCACTGTTTCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGCTACGacatt                             >  1:376002/1‑145 (MQ=255)                                                                                                                 gtCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGGTGGGCATTTATTACTGCCTATCCCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCACGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAatatat                           <  2:679318/149‑1 (MQ=255)                                                                                                                    tACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTTGTATGCCTTCCTCGGATACGAATAtattat                        >  2:995201/1‑149 (MQ=255)                                                                                                                    tACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATTCGattatattat                        >  2:638248/1‑149 (MQ=255)                                                                                                                    cactcgTGGTGAACAAGTTCGCTGGGCGGTGGGTGGGCATTTATTACTGCCTATACCTACGGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATAtattat                        <  1:966202/145‑1 (MQ=255)                                                                                                                     aCGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTCTGCCTTCCTCGGATACGTATATATtgtg                       >  1:1112664/1‑149 (MQ=255)                                                                                                                         gTGGGTAAAAAGTCCGCTGGGCGGAGGTTGGGCATTTATGACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGc                   >  1:702586/1‑149 (MQ=255)                                                                                                                             gTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATAGATTATGACGCCGGt               >  2:858122/1‑149 (MQ=255)                                                                                                                              tAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGCTACGAATATATTATGACGCCGGtt              >  1:920229/1‑149 (MQ=255)                                                                                                                               aaaaaGTTCGCGGGGCGGAGGGTGGCCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTg             <  2:125348/149‑1 (MQ=255)                                                                                                                                aaaaGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGCTACGAATATATTATGACGCCGGTTGc            >  2:1001794/1‑149 (MQ=255)                                                                                                                                aaaaGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGTATATATTATGACGCCGGTTGc            >  2:605326/1‑149 (MQ=255)                                                                                                                                aaaaGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATCTTATGACGCCGGTTGc            >  1:963781/1‑149 (MQ=255)                                                                                                                                      tCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGccaccac      <  2:1074599/149‑1 (MQ=255)                                                                                                                                        gCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCCTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGccaccacca    <  2:1076608/149‑1 (MQ=255)                                                                                                                                          tGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCAtt  <  2:915043/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                             GCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATT  >  CP009273/3219694‑3219979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑