New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 2223931 =53 (0.890)6 (0.110) 5/270 7.7 10.9% intergenic (‑67/‑172) dusC/yohJ tRNA‑dihydrouridine synthase C/UPF0299 family inner membrane protein
?CP009273 2223961 = 49 (0.890)intergenic (‑97/‑142) dusC/yohJ tRNA‑dihydrouridine synthase C/UPF0299 family inner membrane protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGTAT  >  CP009273/2223788‑2224072
                                                                                                                                               |                                                                                                                                             
tAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAggg                                                                                                                                          >  2:129914/1‑149 (MQ=255)
   tCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGAtt                                                                                                                                       <  2:883114/149‑1 (MQ=255)
      ttAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGa                                                                                                                                         >  1:300997/1‑144 (MQ=255)
      ttAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATtgct                                                                                                                                    >  2:606292/1‑149 (MQ=255)
                tCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCaaa                                                                                                                               >  1:122227/1‑144 (MQ=255)
                  aGCAATTCACGCACCAGCGAGCCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACt                                                                                                                             >  1:208814/1‑144 (MQ=255)
                  aGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACt                                                                                                                             >  1:599140/1‑144 (MQ=255)
                    cAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGAGTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATcc                                                                                                                      >  2:404202/1‑149 (MQ=255)
                         cACGCACCAGAGCGTCAAGCCCTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATcc                                                                                                                      <  1:207438/144‑1 (MQ=255)
                            gCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCGAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTAt                                                                                                              >  2:217195/1‑149 (MQ=255)
                                cAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTa                                                                                                               >  1:488626/1‑144 (MQ=255)
                                 agggagTCGAGCCGTCCCTGCATCGGTGCCAATAACACGCGGATATCATAAACAGAAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTAt                                                                                                              <  1:44211/141‑1 (MQ=255)
                                 agagagTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCACAGAGGGATTGCTGCAAACTGCAATCCCTCTCTAt                                                                                                              >  1:322610/1‑144 (MQ=255)
                                           gccccccctccATCGGTGCCAGTAACACCCGCATATCAGCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCt                                                                                               <  2:461527/144‑1 (MQ=255)
                                           gCACTCCCTCCATCGGGGCCAGTAACACACGCCTCGCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCt                                                                                               <  2:301703/149‑1 (MQ=255)
                                                   tccATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGCTGTATGccccaa                                                                                            >  1:100174/1‑140 (MQ=255)
                                                    ccATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAACAGCGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGt                                                                                           <  1:4029/144‑1 (MQ=255)
                                                        cGGTGCCAGTAACACACGCATATCATCGCCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTgcgc                                                                                  <  2:264711/149‑1 (MQ=255)
                                                              cAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTCTGTCTGAGTTCGCTGCGCATTGCg                                                                            >  2:281379/1‑149 (MQ=255)
                                                              cAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCa                                                                                 <  1:371538/144‑1 (MQ=255)
                                                               aGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGt                                                                           >  2:180922/1‑149 (MQ=255)
                                                                  aaCACACGCATATCATCACCCGCAAAAATATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGc                                                                             <  1:398954/144‑1 (MQ=255)
                                                                    cacacGCATATCATCACCCGCAGAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGt                                                                           <  1:590278/144‑1 (MQ=255)
                                                                      cacGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCg                                                                    <  2:585878/149‑1 (MQ=255)
                                                                        cGCATATCATCACCCGGAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCggg                                                                  <  2:471880/149‑1 (MQ=255)
                                                                            tatCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGc                                                              <  2:134502/149‑1 (MQ=255)
                                                                                atcaACAGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAg                                                               <  1:117833/144‑1 (MQ=255)
                                                                                  caCCCGTAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCg                                                             <  1:820676/144‑1 (MQ=255)
                                                                                     ccGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGACCGAAAAAATa                                                     >  2:784647/1‑149 (MQ=255)
                                                                                           aaaaaTGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATacacgcg                                               >  2:868476/1‑143 (MQ=255)
                                                                                           aaaaaTGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCt                                               >  2:603733/1‑149 (MQ=255)
                                                                                           aaaaaTGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCt                                               >  2:542786/1‑149 (MQ=255)
                                                                                                tGAGGCGCTCTGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAGGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCt                                               >  1:445982/1‑144 (MQ=255)
                                                                                                 taGGCGCTAACATAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGAGCTg                                              >  1:528879/2‑144 (MQ=255)
                                                                                                     cgcTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTaa                                     <  2:320319/149‑1 (MQ=255)
                                                                                                              aGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAATGTGATCgg                            <  2:357729/149‑1 (MQ=255)
                                                                                                                  ccTATGGTCCGCTGATTAAAGTTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCCATGCTTGATGTATGCCTGAGTTCGCTGCTCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAATGTGATCGGTAGc                        <  2:336353/149‑1 (MQ=255)
                                                                                                                       ggTCAGCGGATTAACGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAATGTGATCGGTAGCACGtt                   <  2:661650/149‑1 (MQ=255)
                                                                                                                        gcacgcagATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAATGTGATCGGTAGCACGttt                  <  2:954439/142‑1 (MQ=255)
                                                                                                                        gTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGCTTTCTTAATGTGCTCGGTAGCCCGgtt                  >  2:182108/1‑149 (MQ=255)
                                                                                                                        gTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAATGTGATCGGTAGCACGttt                  >  2:956888/1‑149 (MQ=255)
                                                                                                                          cAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAATGTGATCGGTAGCACGTTTTa                <  2:324250/149‑1 (MQ=255)
                                                                                                                            gCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCCTGATGGATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAATGTGATCGGTAGCACGtt                   <  1:807420/144‑1 (MQ=255)
                                                                                                                                   aaaGGTCTGCACAGAGGGATTGCTGCAAACACCAATCCATTTGGATGCTTGATGTATGCCTGAGATCGCCGAGGATTGCTTAATTTCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAATGTGATCGGTAGCACGTTTTACGaa            <  1:382511/144‑1 (MQ=255)
                                                                                                                                    aaGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAATGTGATCGGTAGCACGTTTTACGaattaatt      <  2:596007/149‑1 (MQ=255)
                                                                                                                                    aaGGTATGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAATGTGATCGGTAGCACGTTTTACGaattaatt      <  2:227379/149‑1 (MQ=255)
                                                                                                                                     atgTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAATGTGATCGGTCGCCCGTTTTATGaatt          >  1:394420/3‑144 (MQ=255)
                                                                                                                                     aGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGAAGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAAGAAGCGCTCATGTCTTAATGTGAGCGGTAGCACGTTTTACGaatt          >  1:449559/1‑144 (MQ=255)
                                                                                                                                      ggTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTTCGTAATGCCGGGGAGCCAAAAAAAAAGCGCTGATTTCTTAATGTGATCGGTAGCACGTTTTACGactta         >  1:859460/1‑144 (MQ=255)
                                                                                                                                       gTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAATGTGATCGGTAGCACGTTTTACGCATTAATTGTa   >  2:259834/1‑149 (MQ=255)
                                                                                                                                       gTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGt  >  2:259676/1‑148 (MQ=255)
                                                                                                                                        tCTGGAAAGAGGGATTGCCGCAAACTCCAATGCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGAATGGCGTAATGCCGTTTACCGAAAAAATAAGCGCTGATTTCTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGTat  <  2:756625/149‑1 (MQ=255)
                                                                                                                                            cAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAATGTGATCGGTAGCACGGTTTACGAATTAATTGTa   >  1:743019/1‑144 (MQ=255)
                                                                                                                                               |                                                                                                                                             
TAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGTAT  >  CP009273/2223788‑2224072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.