Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,419,720 T→C D183G (GAT→GGT)  hisJ ← histidine/lysine/arginine/ornithine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,419,7200TC98.4% 196.2 / NA 61D183G (GAT→GGT) hisJhistidine/lysine/arginine/ornithine transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (23/37);  minor base G (0/1);  total (23/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCTTCGCGCAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATA  >  CP009273/2419575‑2419855
                                                                                                                                                 |                                                                                                                                       
tGCTTCGCGCAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTCGCAGCGACCTCACCCt                                                                                                                                      <  2:657300/149‑1 (MQ=255)
       cgcAGTTCGTTATCTTCTTTACGCGGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAAcgc                                                                                                                               >  2:766711/1‑149 (MQ=255)
        gcAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAAcgcg                                                                                                                              <  2:225949/149‑1 (MQ=255)
        gcAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAAcgcg                                                                                                                              <  2:312718/149‑1 (MQ=255)
         cAGTTCCTTATCCTCTTCACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTCACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCgg                                                                                                                             <  2:295922/149‑1 (MQ=255)
           gTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAAcg                                                                                                                                <  1:368086/144‑1 (MQ=255)
             tCGTTTTCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATc                                                                                                                         <  2:163645/149‑1 (MQ=255)
               gTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGCCAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGCCCTCACCCTGGAACGCGGCGTCaa                                                                                                                       >  2:389130/1‑149 (MQ=255)
                ttATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAAt                                                                                                                      <  2:828411/149‑1 (MQ=255)
                 tATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTATGAAGAGACCTTCTCTGGCAGCGACCTCACCCTGGAACGCGGCAt                                                                                                                          >  1:444258/1‑144 (MQ=255)
                    cttcttTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAACCCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGt                                                                                                                  >  2:474691/1‑149 (MQ=255)
                    cttcttTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCaa                                                                                                                       >  1:729220/1‑144 (MQ=255)
                     ttcttcACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAAt                                                                                                                      <  1:340463/144‑1 (MQ=255)
                     ttcttTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAAt                                                                                                                      <  1:929589/144‑1 (MQ=255)
                             gCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCgg                                                                                                              <  1:785845/144‑1 (MQ=255)
                               aGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATCTGTAATCTTTACCGACAGGTTGTTCGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGCAACGCGGCATCAATACGTCCGGCAgtcag                                                                                                       <  2:671393/149‑1 (MQ=255)
                                 gCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCCCCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGCCCGCGACCACACCCTGGAACGCGGCATCAATACGTCCGGCAgt                                                                                                          >  1:369047/1‑144 (MQ=255)
                                 gCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAAACTTTACCGACAGATTGTTAGAGTAACCCATCGCGCACACCAACCCCCCCCTGGAACGCCGAATCCATGCGTCCAGAAatcagta                                                                                                     >  2:216588/1‑147 (MQ=255)
                                   ccATGCCGGTCCCCACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACTTAAGCCGAGGACGCGGCATCAATACGTCCGGCAgtcaggtca                                                                                                   <  2:505956/149‑1 (MQ=255)
                                     aTGCCGGTCCCTACGCAAAACAGTTTTTCATCTTTAAAAGACGAGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGa                                                                                                 >  2:657220/1‑149 (MQ=255)
                                       gCCGGTCCCTCCGCCAAATAGTTTTTCATCTTTAACAGACGGGGCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAgtcaggtc                                                                                                    <  1:789315/144‑1 (MQ=255)
                                          ggTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATTCGTCCGGCAGTCAGGTCAGAATaaa                                                                                            <  2:518107/149‑1 (MQ=255)
                                           gTCCCTACGCCAAACATTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAAt                                                                                           <  2:356757/149‑1 (MQ=255)
                                              ccTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATACAtgtt                                                                                        >  2:114999/1‑149 (MQ=255)
                                               cTACGCCAAACAGTTTTTCATCTGTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATaaa                                                                                            <  1:152606/144‑1 (MQ=255)
                                                tACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAAACTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAAtgttgt                                                                                      >  2:970813/1‑149 (MQ=255)
                                                   gCCAACCAGTTTTTCACCTTTATCAGACGGGCCACCGAATTTGTAATCTTGACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCTACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCGGGATAAAtgtt                                                                                        <  1:625905/144‑1 (MQ=255)
                                                   gCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAAtgtt                                                                                        <  1:864647/144‑1 (MQ=255)
                                                      aaaCAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGcc                                                                                <  2:189969/149‑1 (MQ=255)
                                                      aaaCAGGTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTc                                                                                     <  1:171001/144‑1 (MQ=255)
                                                                tCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGa                                                                           <  1:93729/144‑1 (MQ=255)
                                                                tCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACga                                                                      >  2:550185/1‑149 (MQ=255)
                                                                tCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGGTGTCCTGCCCCTGa                                                                           >  1:846224/1‑144 (MQ=255)
                                                                tCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGACAAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACga                                                                      >  2:458810/1‑149 (MQ=255)
                                                                 cATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACgag                                                                     <  2:798135/149‑1 (MQ=255)
                                                                       tAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGAtt                                                               <  2:921658/149‑1 (MQ=255)
                                                                          cAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGa                                                                 >  1:850466/1‑144 (MQ=255)
                                                                            gACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAAt                                                          >  2:331478/1‑149 (MQ=255)
                                                                               gggCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCa                                                            <  1:251492/144‑1 (MQ=255)
                                                                                  ccACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCttt                                                    >  2:49138/1‑149 (MQ=255)
                                                                                    aCCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTg                                                  <  2:1000337/149‑1 (MQ=255)
                                                                                     ccGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTgg                                                 <  2:87298/149‑1 (MQ=255)
                                                                                      cGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCtt                                                     >  1:179104/1‑144 (MQ=255)
                                                                                             gTAATCTTTAGCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGc                                              <  1:22551/144‑1 (MQ=255)
                                                                                             gTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGgcgc                                              >  1:765215/1‑144 (MQ=255)
                                                                                               aaTCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGccc                                            >  1:38263/1‑144 (MQ=255)
                                                                                                        cGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCg                              <  2:662850/149‑1 (MQ=255)
                                                                                                                tgttTGAGGAAACCTTCGCGGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAAc                           <  1:425781/144‑1 (MQ=255)
                                                                                                                 gttTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTcc                     <  2:399585/149‑1 (MQ=255)
                                                                                                                  tttGAGCAAACCTTCGCTGGCAGCGACATCTCCCTCGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCt                    <  2:192541/149‑1 (MQ=255)
                                                                                                                         aaaCCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTgg                  >  1:316871/1‑144 (MQ=255)
                                                                                                                            tcTTCGCTGGCAGCGACTTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGggtg               <  1:428290/143‑1 (MQ=255)
                                                                                                                              ttCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGTCAGCCAGGTCAGAATAAATGTTATCCTGCCCCTCATACAAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTTGGTGGTGCCCt        >  2:595549/1‑149 (MQ=255)
                                                                                                                              ttCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGggtggt             >  1:707171/1‑144 (MQ=255)
                                                                                                                              ttCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCt        <  2:926065/149‑1 (MQ=255)
                                                                                                                              ttCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTCCAAGGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCt        <  2:805761/149‑1 (MQ=255)
                                                                                                                               tCGCTGGCAGCTGCCGCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAACGTTCATGACCGAACGTCTCCTGGGTGGTGCCCTg       <  2:149274/149‑1 (MQ=255)
                                                                                                                                 gCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAAGGCAAAATAAATGTTGTCCTGCCACTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTGATTACCGAACGTCCCCTGGGTGGTGCCCTGCa     >  2:459444/1‑149 (MQ=255)
                                                                                                                                  cttgCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGccc         <  1:493667/141‑1 (MQ=255)
                                                                                                                                  cTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGCCGATTGTAATGCCTTTTGGTTCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCaa    <  2:502246/149‑1 (MQ=255)
                                                                                                                                         cgcacTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATa  <  1:820748/140‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                       
TGCTTCGCGCAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATA  >  CP009273/2419575‑2419855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑