Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	4,048,383	C→T	D86N (GAT→AAT)	glnG ←	fused DNA‑binding response regulator in two‑component regulatory system with GlnL: response regulator/sigma54 interaction protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,048,383	0	C	T	100.0%	320.5 / NA	96	D86N (GAT→AAT)	glnG	fused DNA‑binding response regulator in two‑component regulatory system with GlnL: response regulator/sigma54 interaction protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/58);  total (38/58)

TTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACT  >  CP009273/4048238‑4048525                                                                                                                                                  |                                                                                                                                               ttAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCCGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGa                                                                                                                                             <  1:148696/149‑1 (MQ=255) ttAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGa                                                                                                                                             >  1:329368/1‑149 (MQ=255)  tAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGaa                                                                                                                                            <  1:320157/149‑1 (MQ=255)      cTGAACATTCCGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAAtgtg                                                                                                                                        <  2:85528/149‑1 (MQ=255)      cTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAAtgtg                                                                                                                                        <  1:1585732/149‑1 (MQ=255)      cTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCCCAAGCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAAtgtg                                                                                                                                        <  1:960884/149‑1 (MQ=255)      cTGAACATTACGCCGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAAtgtg                                                                                                                                        >  1:1080162/1‑149 (MQ=255)        gAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCg                                                                                                                                      >  1:1241625/1‑149 (MQ=255)        gAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACAGCAGCATCCAGATTGGAAtgtggg                                                                                                                                      >  1:1379858/1‑147 (MQ=255)        gAACATTACGCGGATGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTTCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACCCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCg                                                                                                                                      >  1:1087282/1‑149 (MQ=255)           cATTCCGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTc                                                                                                                                   <  2:927457/149‑1 (MQ=255)           cATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTc                                                                                                                                   <  2:1736578/149‑1 (MQ=255)            aTTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAAACCGCTCAACCAGCGCCACTGCTTCGTCGCTATCAACCGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCAGCCAGATTGGCCTGGGCGGTCa                                                                                                                                  >  1:16317/1‑149 (MQ=255)            aTTACGCGGCGGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCa                                                                                                                                  <  1:569156/149‑1 (MQ=255)              tACGTGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGTAGCATCCAGATTGGAATGTGCGGTCATa                                                                                                                                <  1:1304448/149‑1 (MQ=255)               aCGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATaa                                                                                                                               <  2:926201/149‑1 (MQ=255)                 gcgGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAatg                                                                                                                             >  2:1622040/1‑149 (MQ=255)                 gcgGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAatg                                                                                                                             <  2:1841832/149‑1 (MQ=255)                    gctgctgctGTTCCTGGTAATGACGGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAatgatg                                                                                                                          <  1:9910/149‑1 (MQ=255)                      tgctgctgTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGAc                                                                                                                        <  1:1620515/149‑1 (MQ=255)                       gctgctgTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATCTCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGCATGTGCGGTCATAATGATGAcc                                                                                                                       >  1:751902/1‑149 (MQ=255)                       gctgctgTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCGGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGAGGCATCCAGATTGGAATGTGCGGTCATAatgatagcc                                                                                                                       >  1:192409/1‑145 (MQ=255)                       gctgctgTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATCATTATGAcc                                                                                                                       <  1:233099/149‑1 (MQ=255)                         tgctgTTCCTGGGAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTGATAAAGAGGACAgg                                                                                                                     >  1:324426/1‑149 (MQ=255)                         tgctgTCCCTGGTAATGGCTGGTAGCGCGCTCAACCAGCGCCACTTCTTCGTCGATATCAAACGGTGTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAAGGTGCGGTCATCATTATGACCgc                                                                                                                     <  2:934831/149‑2 (MQ=255)                           ctgTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGaa                                                                                                                   <  2:1589319/149‑1 (MQ=255)                           ctgTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGCTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGCTTGGAATGTGCGGTCATAATGATGCCCGGaa                                                                                                                   >  2:1304167/1‑149 (MQ=255)                            tgTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAg                                                                                                                  <  2:1529512/149‑1 (MQ=255)                              ttCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGcg                                                                                                                >  1:873554/1‑148 (MQ=255)                              ttCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCa                                                                                                                >  1:1240169/1‑149 (MQ=255)                               tCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCCTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCAt                                                                                                               <  2:1700746/149‑1 (MQ=255)                               tCCTGGTAACGAGTGTTAGCGCGTGCAACCAGCGCCACTGCTTCGTCGCTATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCAt                                                                                                               <  1:803865/149‑1 (MQ=255)                                 cTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGCGCGGTCATAATGATGACCGGAAGCATTg                                                                                                             >  1:1375177/1‑149 (MQ=255)                                  tGGTAATGACTGATCGCGCGCTCAACCAGCGCCACTGCTTCGTTGATATCAATCATATTCGGGAGATATTCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTgg                                                                                                            <  2:371061/149‑1 (MQ=255)                                  tGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGCTAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTgg                                                                                                            >  2:1798666/1‑149 (MQ=255)                                  tGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTgg                                                                                                            >  1:242090/1‑149 (MQ=255)                                   ggTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGa                                                                                                           <  1:735004/149‑1 (MQ=255)                                   ggTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGa                                                                                                           <  1:1502107/149‑1 (MQ=255)                                      aaTGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATgg                                                                                                        <  1:1160285/149‑1 (MQ=255)                                               tggcgcgcTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATTAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTa                                                                                               <  1:1073142/147‑1 (MQ=255)                                                aGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTaa                                                                                              <  2:1224039/149‑1 (MQ=255)                                                 gcgcgcTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAAt                                                                                             <  1:1568585/149‑1 (MQ=255)                                                 gcgcgcTCAACCAGCGCCACTGCTTCGTCGATACTAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCGGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAAt                                                                                             <  1:483838/149‑1 (MQ=255)                                                 gcgcgcCCAACCCGCGCCCCTGCTTCGTCGATATCAAACGGTTTGGGCAGAGAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAAt                                                                                             <  2:474313/149‑1 (MQ=255)                                                  cgcgcTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATc                                                                                            <  2:1049102/149‑1 (MQ=255)                                                  cgcgcTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACCCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCCTTGGATGGCGCTGTTTAATc                                                                                            >  2:1385382/1‑149 (MQ=255)                                                    cgcTCAACCAGAGCCCCTGCTTCGTCTGTACAAAACGGTTTGGGCAGATAAGCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTg                                                                                          <  1:1613526/149‑1 (MQ=255)                                                     gcTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCTGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGc                                                                                         <  2:862802/149‑1 (MQ=255)                                                     gcTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGc                                                                                         >  1:680910/1‑149 (MQ=255)                                                     gcTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGc                                                                                         <  1:970502/149‑1 (MQ=255)                                                       tcgaCCAGCGCCACTGACTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCCGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCtt                                                                                       <  1:1196892/146‑1 (MQ=255)                                                       tCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAGCGCCCCTTGTTGATAGGCGCTGACGGGAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCtt                                                                                       <  1:850433/149‑1 (MQ=255)                                                       tCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCtt                                                                                       <  2:301841/149‑1 (MQ=255)                                                       ccAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCtt                                                                                       <  1:1445480/148‑1 (MQ=255)                                                         caCCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGa                                                                                     <  1:1628105/148‑1 (MQ=255)                                                          aCCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATTTGCTTGag                                                                                    >  1:574436/1‑149 (MQ=255)                                                          aCCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGag                                                                                    <  1:1518872/149‑1 (MQ=255)                                                           ccAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGagc                                                                                   >  1:267744/1‑149 (MQ=255)                                                           ccAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCAACCAGCTTGGAAAGTGCGGTCATAATGATGACCGGAAGCCTTGGAAGGCGCCGTTTTAGCCGCTTGagc                                                                                   >  1:93283/1‑149 (MQ=255)                                                                gcCACTGCTTCGTCGATACCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAgcgc                                                                              <  2:1469442/149‑1 (MQ=255)                                                                 ccACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGcc                                                                             >  1:1015290/1‑149 (MQ=255)                                                                 ccACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATATGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGcc                                                                             >  1:10944/1‑149 (MQ=255)                                                                 caaCTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATCGCGGCGTCTAATCTGGTTGAGCAGCGcc                                                                             >  1:143604/3‑149 (MQ=255)                                                                   gcTGCGTCGGCGATAGCAAACGGTGGGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACTGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAg                                                                           <  1:1642336/148‑1 (MQ=255)                                                                    cTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGc                                                                          <  1:467370/149‑1 (MQ=255)                                                                      gCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGccc                                                                        <  2:1066212/149‑1 (MQ=255)                                                                        ttCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGCTTGGAATGTGCGGTCGTAATGATGACCGGAAGCATTGGATGGGGCTGTTTAATCTACTTGGGCGACGCCAGCCCGt                                                                      >  1:1094020/1‑149 (MQ=255)                                                                         tcgtcgATATCAAACGGTTTGGGCAGATAATCAAACGTCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTc                                                                     <  2:1264905/149‑1 (MQ=255)                                                                         tcgtcgATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGGTGACCGGAAGACTCCGAAGGCGCGGGTTACGCTGCTTGAGCCGCGCCAggtcggc                                                                     >  2:1793544/1‑143 (MQ=255)                                                                         tcgtcgATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTc                                                                     <  2:263440/149‑1 (MQ=255)                                                                           gtcgATATCAAACGGTTTGGGCAGATCATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCa                                                                   <  2:1558296/149‑1 (MQ=255)                                                                                 atgaaaCGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCg                                                             <  1:238992/146‑1 (MQ=255)                                                                                 atCAAACGGTTTGGGCAGATAATCATACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCg                                                             <  2:1189600/149‑1 (MQ=255)                                                                                 agcAAACGGTTTGGTCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCg                                                             <  2:194951/147‑1 (MQ=255)                                                                                               gCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCCGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCt                                               >  2:1284287/1‑149 (MQ=255)                                                                                                cAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTg                                              >  1:1573042/1‑149 (MQ=255)                                                                                                  gATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTACCGGCATCCGGATATCGGaa                                            >  1:1005334/1‑149 (MQ=255)                                                                                                   aTAATCAAACGCCCCTTGTTGATAGGCACTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGaaa                                           <  2:1350752/149‑1 (MQ=255)                                                                                                      aTCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCACCCCGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAagca                                        <  2:838917/149‑1 (MQ=255)                                                                                                       tCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAagcag                                       <  2:357231/149‑1 (MQ=255)                                                                                                           aCGCCCCTTGTTGATAGGCGCTGCCGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCACACGGATATCTGAAAGCAGcacg                                   >  1:418005/1‑148 (MQ=255)                                                                                                            cGCCCCTTGTTGATAGGCGCTGACGGCAGCAGCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACAt                                  <  2:1166793/149‑1 (MQ=255)                                                                                                                 cTTGTTGATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGc                             >  1:1498998/1‑149 (MQ=255)                                                                                                                  ttgttgATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCCCATCCGGCg                            >  1:852102/1‑149 (MQ=255)                                                                                                                  ttgttgAGAGGCGCTGACGGCAGCATCCAGATTGGAAGGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCg                            <  1:763638/149‑1 (MQ=255)                                                                                                                    gttcATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGAGGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCACACGGATAGCTGAAAGCAGCGCATCCGGCgat                          >  1:1174554/1‑147 (MQ=255)                                                                                                                     ttgATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGttt                         <  2:290718/149‑1 (MQ=255)                                                                                                                     ttgATAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGttt                         <  1:1671655/149‑1 (MQ=255)                                                                                                                         tAGGCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCt                     >  1:1792733/1‑149 (MQ=255)                                                                                                                          aGGCGCGGACGGAAGCATCCAAATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTc                    <  2:12574/149‑1 (MQ=255)                                                                                                                           ggCGCTGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGGGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCg                   >  1:1747147/1‑149 (MQ=255)                                                                                                                                tGACGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTcacgatc              >  2:1305575/1‑143 (MQ=255)                                                                                                                                  aCGGCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGAAATCTGAAAGCAGCACATCCGGCGCTTTGCCCGCCCgcgc            >  1:127137/1‑149 (MQ=255)                                                                                                                                    ggCAGCATCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCt          <  2:1095572/149‑1 (MQ=255)                                                                                                                                       agcaTCCAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCa       <  2:1696291/149‑1 (MQ=255)                                                                                                                                            ccAGATTGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCACGCGTTTTGCTCGCCAGCGCGCCCAGCGCt  >  1:1435198/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                               TTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACT  >  CP009273/4048238‑4048525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑