Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,621,613 A→T 4.5% L429* (TTA→TAA)  rbbA ← ribosome‑associated ATPase: ATP‑binding protein/ATP‑binding membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,621,6130AT 4.5% 277.9 / 2.6 90L429* (TTA→TAA) rbbAribosome‑associated ATPase: ATP‑binding protein/ATP‑binding membrane protein
Reads supporting (aligned to +/- strand):  ref base A (41/43);  new base T (2/2);  total (45/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-01

GCGGTCGCAACGTTCCGCTTCGTTCATAAAGTGGGTGGAGATGAAGATAGTCACTTTGTCCTGGCGCGAGAGATCGACCATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATCTTCAACGTCGTTGAGCTTAAAACGCTCGCTCATTTCAGCCACTCTTGCGGGAATTTCCGCTTCCG  >  CP009273/3621471‑3621760
                                                                                                                                               |                                                                                                                                                   
gCGGTCGCAACGTTCCGCTTCGTTCATAAAGTGGGTGGAGATGAAGATAGTCACTTTGTCCTGGCGCGAGAGATCGACCATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCCCATCGAGGATTa                                                                                                                                                     >  2:294824/1‑143 (MQ=255)
 cGGTCGCAACGTTCCGCTTCGTTCATAAAGTGGGTGGAGATGAAGATAGTCACTTTGTCCTGGCGCGAGAGATCGACCATCAACTGCCAGAACATCTCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACCtctc                                                                                                                                              >  1:176332/1‑149 (MQ=255)
  ggTCGCAACGTTCCGCTTTGTTCATAAAGTGGGTGGAGATGAGGTTAGTCACTTTGTCCTGGCCCGAGAGCTCGACCATTTTTTTTTTAATGATA‑CGGCGACCACCGAGATCTACACCAGAAGTAGGCTCATCGAGGATTAAc                                                                                                                                                   <  2:130599/143‑1 (MQ=255)
  ggTCGCAACGTTCCGCTTCGTTCATAAAGTGGGTGGAGATGAAGATAGTCACTTTGTCCTGGCGCGAGAGATCGACCATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAAc                                                                                                                                                   <  2:91489/143‑1 (MQ=255)
    tCGCAACGTTCCGCTTCGTTCATAAAGTGGGTGGAGATGAAGATAGTCACTTTGTCCTGGCGCGAGAGATCGACCATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGAGTAGCGTCTCCgg                                                                                                                                           >  1:139469/1‑149 (MQ=255)
    tCGCAACGTTCCGCTTCGTTCACAAAGTGGGTGGAGATGAAGATAGTCACTTTGTCCTGGCGCGAGGGATCGACCATCAACTGCCAGAACATATCCCCCGCCACCG‑GATCGACACCAGAAGTAGGCTCAACGAGGATTATCATATCCgg                                                                                                                                           >  1:367170/1‑149 (MQ=255)
     cGCAACTTTCCGCTTCGTTCATAAAGTGGGTGTAGATGAAGATAGTCACTTTGTCCTGGCGCGAGAGATCGACCATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCggg                                                                                                                                          <  1:191482/149‑1 (MQ=255)
        aaCGTTCCGCTTCGTTCATAAAGTGGGTGGAGATGAAGATAGTCACTTTGTCCTGGCGCGAGAGATCGACCATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGa                                                                                                                                       <  1:180930/149‑1 (MQ=255)
               cGCTTCGTTCATAAAGTGGGTGGAGAGGAAGATAGTCACTTTGTCCTGGCGCGAGCGATCGACCATCATTTGCCAGATCATACCGCGCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGAt                                                                                                                                      <  2:106284/143‑1 (MQ=255)
                gCTTCGTTCATAAAGTGGGTGGAGATGAAGATAGTCACTTTGTCCTGGCGCGAGAGATCGACCATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTGACATCTCCGGGCGagg                                                                                                                                     >  2:76195/1‑141 (MQ=255)
                gCTTCGTTCATAAAGTGGGTGGAGATGAAGATAGTCACTTTGTCCTGGCGCGAGAGATCGACCATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATcac                                                                                                                               <  1:325021/149‑1 (MQ=255)
                 cTTCGTTCATAAAGTGGGTGGAGATGAAGATAGTCACTTTGTCCTGGCGCGAGAGATCGACCATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATcacc                                                                                                                              <  1:134831/149‑1 (MQ=255)
                   tCGTTCATAAAGTGGGTGGAGATGAAGATAGTCACTTTGTCCTGGCGCGAGAGATCGACCATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAAt                                                                                                                                  <  2:2283/143‑1 (MQ=255)
                   tCGTTCATAAAGTGGGTGGAGATGAAGATAGTCACTTTGTCCTGGCGCGAGAGATCGACCATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATcaccgc                                                                                                                            <  1:93680/149‑1 (MQ=255)
                     gttgaaaaaGTGGGTGGAGATGAAGGCAGTCCCTTTGTCCGGGCGCGCGCGATCGACCATCAACTGCCAGAACATATCCCTCGCCCCCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATca                                                                                                                                <  2:182794/137‑1 (MQ=255)
                      ttCATAAAGTGGGTGGAGATGAAGATTTTCACTTTGTCCTGGCGCGAGAGATCGACCATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTCGGCTCATCGAGGATTAACATCTCCGGCCGATGAATcac                                                                                                                               >  2:250280/1‑143 (MQ=255)
                      ttCATAAAGTGGGTGGAGATGAAGATAGTCGCTTTGTCCTGGCGCGAGAGATCGACCATCAACTGCCAGCACATCTCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTCACATCTCCGGGCGCTGAATgacggccgc                                                                                                                         >  1:35613/1‑147 (MQ=255)
                       tCATAAAGTGGGTGGAGATGAAGATAGTCACTTTGTCCTGGCGCGAGAGATCGACCATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATcaccgccacc                                                                                                                        <  1:123442/149‑1 (MQ=255)
                       tCATAAAGTGGGTGGAGATGAAGATAGTCACTTTGTCCTGGCGCGAGAGATCGACCATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATcaccgccacc                                                                                                                        <  1:366263/149‑1 (MQ=255)
                        cATAAAGTGGGTGGAGATGAAGATAGTCACTTTGTCCTGGCGCGAGAGATCGACCATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGGGATGAATaaccggcaccg                                                                                                                       >  1:118467/1‑149 (MQ=255)
                            aaGGGGGTGGAGATGAAGATAGTCACTTTGTCCTGGCGCGAGAGATCGACCATCAATTGCCAGAACATACCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATcaccgccac                                                                                                                         <  2:270919/143‑1 (MQ=255)
                              gtgggtggAGATGAAGATAGTCACTTTGTCCTGGCGCGAGAGATCGACCATCACCTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTGCGCTCATCGAGGAGTAACAACCCCCGGCGATGAATcaccaccaccg                                                                                                                       >  2:180394/1‑143 (MQ=255)
                              gtgggtggAGATGAAGATAGTCACTTTGTCCTGGCGCGAGAGATCGACCATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTTACATCTCCGGGCGATGAATcaccgccaccg                                                                                                                       >  2:330185/1‑143 (MQ=255)
                              gtgggtggAGATGAAGATAGTCACTTTGTCCTGGCGCGAGAGATCGACCATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTTACATCTCCGGGCGATGAATcaccgccaccg                                                                                                                       >  2:261509/1‑143 (MQ=255)
                                 ggtggAGATGAAGATAGTCACCTTGTCCTGGCGCGAGAGATCGACCATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGaaa                                                                                                              <  1:5831/149‑1 (MQ=255)
                                     gagaTGAAGATAGTCACTTTGTCCTGGCGCGAGAGATCGACCATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGCAAGCCg                                                                                                          >  1:171324/1‑149 (MQ=255)
                                     gagaTGAAGATAGTCACTTTGTCCTGGCGCGAGAGATCGACCATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCg                                                                                                          >  1:323390/1‑149 (MQ=255)
                                         tGAAGATAGGCACTTTGTCCTGGCGCGAGAGATCGACCATCAACTGCCAGCACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTgg                                                                                                      <  1:169388/149‑1 (MQ=255)
                                                aGTCACTTTGTCCTGGCGCGAGAGATCGACCATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGCCACCAGAAGAAGGCTCATCGAGGATTAACACCTCCGGGCGATGAATCACCACCCCCGCCCGCCGACGCCGCGTGCGAAGGc                                                                                               >  1:148232/1‑149 (MQ=255)
                                                 gTCACTTTGTCCCGGCGCGAGAGATCGACCATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGcc                                                                                              <  1:229664/149‑1 (MQ=255)
                                                     cattaaacTGGCGCGAGAGATCGACCATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCCGAAGTAGGCTCATCGAGGAGTAACATCTCCGGGCGATGAATCACCGCCACCCCCCGCGAAAACCGCTGGCGAATg                                                                                                >  2:86846/8‑143 (MQ=255)
                                                     cTTTGGCCGGCCGCGAGCCATCGACCAGCAACTGCCAGAACATATCCCTCGCCCCCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATg                                                                                                <  2:25177/143‑1 (MQ=255)
                                                      tttGTCCTGGCGCGAGAGATCGACCATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACGCCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGTAACCCGCTGGCGAATGCCggggg                                                                                         >  1:188916/1‑145 (MQ=255)
                                                      tttGACCTGGCGCGAGAGATCGACCATCAACTTCCACAACATCTCCCGCCCCCCCG‑GACCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGc                                                                                               <  2:127678/143‑1 (MQ=255)
                                                        tggccTGGCGCGAGAGATCGACCATCAACTGCCAGAAAATATCCCTCGCCACCG‑GATCGACACCAGAAGTAAGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGCCGCATGCCg                                                                                             >  2:314015/4‑143 (MQ=255)
                                                        tGTCCTGGCGCGAGAGATCGACCATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGc                                                                                       >  1:234842/1‑149 (MQ=255)
                                                        tGTCCCGGCGCGAGAGATCGACCATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGc                                                                                       <  1:63855/149‑1 (MQ=255)
                                                         gTCCTGGCGCGAGAGATCGACCATCAACTGCCAGAACAGAGCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCa                                                                                      <  1:159589/149‑1 (MQ=255)
                                                            cTGGCGCGAGAGATCGACCATGAACTGCCAGAACATATCCCTCGCCACCG‑GATCGAGACCAGGAGTGGGCTCATCGAGGATTAACATCTCCGGGCGATGAGTCACCGCCCCCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCg                                                                                         >  2:106807/1‑143 (MQ=255)
                                                             tGGCGCGAGTGATCGACCATCAACTGCCAGAACATAGCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGa                                                                                  <  1:242008/149‑1 (MQ=255)
                                                             tGGCGCGAGAGATCGACCATCAACTGCCAGAACATATCCCTCGCCAGCG‑GATCGGCACCAGAAGTAGGCGCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACGGCCAGCGAAAGCCGCTGGCGAATGCCGAGCgg                                                                                        >  2:103494/1‑143 (MQ=255)
                                                             tGGCGCGAGAGATCGACCATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCGGGCGAATGCCGAGCGGCAATGa                                                                                  >  1:151318/1‑149 (MQ=255)
                                                             tGGCGCGAGAGATCGACCATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATAGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGa                                                                                  >  1:325299/1‑149 (MQ=255)
                                                               gcgcGAGAGATCGACCATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCCCATCGAGGATTAACATCTCCGGGCGATGAATCCCCGCCACCGCCCGCGAAAGCCGCTGGCGACTGGCGAGCGGcc                                                                                      >  2:87147/1‑142 (MQ=255)
                                                                 gcgAGAGATCGACCATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCGGCGAAAGCCGCTGCCGAATGCCGCGCGGCAAt                                                                                    >  2:109786/1‑143 (MQ=255)
                                                                   gagagaTCGACCATTCACGTCCAGAACATATCCCTCGCCACCG‑GATCGACCCCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAAGCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGa                                                                                  <  2:14442/143‑1 (MQ=255)
                                                                    agagaTCGACCATCAACTGCCAGAACATATCCCTCGCTACCG‑GATCGATACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTCGCGAATGCCGAGCGGCAATGACTCCGGc                                                                           <  1:240611/149‑1 (MQ=255)
                                                                    agagaTCGACCATCAACTGCAAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCgga                                                                           >  1:357806/1‑148 (MQ=255)
                                                                        aTCGACCATCAACTGCCAGAACATATCCCTCGCCCCCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCg                                                                             <  2:186603/143‑1 (MQ=255)
                                                                           gACCATCAACTGCCACAACATATCCCCCCCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCa                                                                          <  2:25253/143‑1 (MQ=255)
                                                                            aCCATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATc                                                                   >  1:148916/1‑149 (MQ=255)
                                                                              cATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGaa                                                                       <  2:209534/143‑1 (MQ=255)
                                                                              cATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGATCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATCtt                                                                 >  1:346533/1‑149 (MQ=255)
                                                                               aTCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACCCCGCCAGAat                                                                      >  2:260455/1‑143 (MQ=255)
                                                                               aTCAACTGCCAGAACATATCCCCCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACACCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATCTTc                                                                <  1:356285/149‑1 (MQ=255)
                                                                                    ttGCCAGAACATATCCCGCGCCGCCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATCtt                                                                 <  2:155488/142‑1 (MQ=255)
                                                                                     tGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATCTTCAAcgtc                                                          >  1:262866/1‑149 (MQ=255)
                                                                                      gCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATCTTCa                                                               <  2:317831/143‑1 (MQ=255)
                                                                                       ccAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATCTTCAAcgtcgt                                                        >  1:201299/1‑149 (MQ=255)
                                                                                        aaGACCACATCCCTCCCCACCG‑GACCGACACCAGAAGTAGGCTCACCGAGGATTACCATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATTTTCAACGTCGtt                                                       <  1:61128/148‑1 (MQ=255)
                                                                                           aaCATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATCTTCAACGTCGTTGAg                                                    >  1:36638/1‑149 (MQ=255)
                                                                                             cATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATCTTCAACGTCGTTGAGCt                                                  >  1:100673/1‑149 (MQ=255)
                                                                                                  cccTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTTACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATCTTCAACGTCGTTGAGCTTaaaa                                             <  1:291363/149‑1 (MQ=255)
                                                                                                  cccTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTTACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATCTTCAACGTCGTTGAGCTTaaaa                                             <  1:365167/149‑1 (MQ=255)
                                                                                                  cccTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATCTTCAACGTCGTTTAGCTTcaaa                                             >  1:139518/1‑149 (MQ=255)
                                                                                                   ccTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATCTTCAACGTCGTTGAGCTTAAAAc                                            <  1:35825/149‑1 (MQ=255)
                                                                                                        ccACCG‑GATCGACACCAGAAGTAGGATCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATCTTCAACGTCGTTGAGCTTAAAAcgctcg                                       <  1:226236/149‑1 (MQ=255)
                                                                                                            cG‑GATCGACACCGGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGCCTCCGTTAGAATATCTTCAACGTCGTTGAGCTTAAAAcgct                                         <  2:233839/143‑1 (MQ=255)
                                                                                                            cG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATCTTCAACGTCGTTGAGCTTAAAAcgct                                         <  2:290496/143‑1 (MQ=255)
                                                                                                               gaccGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGCGCGGCAATGACTCCGGCAGAATATCTTCAACGTCGTTGAGCTTAAAAcgctcg                                       <  2:174415/140‑1 (MQ=255)
                                                                                                               gATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATCTTCAACGTCGTTGAGCTTAAAACGCTCGCTCAtt                                 >  1:243455/1‑149 (MQ=255)
                                                                                                                   gaaacCAGAAGTAGGCTGATGGAGGTTTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCAAATGCCGAGCGGCAATGACTCCGGCAGAATATCTTCAACGTCGTTGAGCTTAAAACGCTCGCTCATTTCAg                             <  1:216814/146‑1 (MQ=255)
                                                                                                                    acacCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATCTTCAACGTCGTTGAGCTTAAAACGCTCGCTCAt                                  >  2:199173/1‑143 (MQ=255)
                                                                                                                    acacCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAAGCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATCTTCAACGTCGTTGAGCTTAAAACGCTCGCTCATTTCAGc                            <  1:171068/149‑1 (MQ=255)
                                                                                                                        cAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATCTTCGACGTCGTTGCGCTTAAAACGCTCGCTCATTTCAGCCAct                        >  1:43842/1‑149 (MQ=255)
                                                                                                                        cAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATCTTCGACGTCGTTGAGCTTAAAACGCTCGCTCATTTCa                              >  2:228224/1‑143 (MQ=255)
                                                                                                                             gTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATCTTCAACGTCGTTGAGCTTAAAACGCTCGCTCATTTCAGCCACTCTTGc                   >  1:50344/1‑149 (MQ=255)
                                                                                                                              tAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATAACTTAAACGTCGTTGAGCTTAAAACGCTCGCTCATTTCAGCCACTCTTGCg                  >  1:69152/1‑149 (MQ=255)
                                                                                                                                ggCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGCATATCTTCAACGTCGTTGAGCTTAAAACGCTCGCTCATTTCAGCCAccct                      >  2:35912/1‑143 (MQ=255)
                                                                                                                                ggCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATCTTCAACGTCGTTGAGCTTAAAACGCTCGCTCATTTCACCCAcact                      >  2:144256/1‑143 (MQ=255)
                                                                                                                                ggCTCACCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATCTTCAACGTCGTTGAGCTTAAAACGCTCGCTCATTTCAGCCACTCTTGCggg                <  1:164916/149‑1 (MQ=255)
                                                                                                                                 gCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGACACAGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACACCGGCAGCATATCTTCAACATCGTGGAGGTCAAAACGCTCACTCATTTCAGCCAATCtt                     >  2:191116/1‑143 (MQ=255)
                                                                                                                                  cTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATCTTCAACGTCGTTGAGCTTAAAACGCTCGCTCATTTCAGCCACTCTTGCGGGaa              >  1:299440/1‑149 (MQ=255)
                                                                                                                                   tgaTCGAGGATGATCATCCCCGGGCGAGCCATCACCGCCAGCGCCAGCGAAAGGCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATCTTCAACGTCGTTGAGCTTAAAACGCTCGCTCATTTCAGCCACTCTTGCGGGAAt             <  1:5101/147‑1 (MQ=255)
                                                                                                                                    cATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATCTTCAACGTCGTTGAGCTTAAAACGCTCGCTCATTTCAGCCACTCTTGCg                  <  2:18867/143‑1 (MQ=255)
                                                                                                                                        gAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATCTTCAACGTCGTTGAGCTTAAAACGCTCGCTCATTTCAGCCACTCTTGCGGGaa              <  2:241476/143‑1 (MQ=255)
                                                                                                                                         aGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATCTTCAACGTCGTTGAGCTTAAAACGCTCGCTCATTTCAGCCACTCTTGCGGGAATTTCCGc       >  1:129274/1‑149 (MQ=255)
                                                                                                                                           gATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATCTTCAACGACGTTTAGGTTAAAACGCTCGCGCATTTCAGCCACTCTTGCGGGAAttt           >  2:242076/1‑143 (MQ=255)
                                                                                                                                             ttAACATCTCCGGGCGATGGATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCCATGACTCCGGCAGAATATCTTCAACGTCGTTGAGCTTCAAACGCTCGCTCATTTCAGCCACTCTTGCGGGAATTTcc         <  2:292053/143‑1 (MQ=255)
                                                                                                                                              tAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATCTTCAACGTCGTTGAGCTTAAAACGCTCGCTCATTTCAGCCACTCTTGCGGGAATTTCCg        >  2:179204/1‑143 (MQ=255)
                                                                                                                                              tAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATCTTCAACGTCGTTGAGCTTAAAACGCTCGCTCATTTCAGCCACTCTTGCGGGAATTTCCGCTTCCg  <  1:220988/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
GCGGTCGCAACGTTCCGCTTCGTTCATAAAGTGGGTGGAGATGAAGATAGTCACTTTGTCCTGGCGCGAGAGATCGACCATCAACTGCCAGAACATATCCCTCGCCACCG‑GATCGACACCAGAAGTAGGCTCATCGAGGATTAACATCTCCGGGCGATGAATCACCGCCACCGCCAGCGAAAGCCGCTGGCGAATGCCGAGCGGCAATGACTCCGGCAGAATATCTTCAACGTCGTTGAGCTTAAAACGCTCGCTCATTTCAGCCACTCTTGCGGGAATTTCCGCTTCCG  >  CP009273/3621471‑3621760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑