| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 788,752 | C→A | 12.1% | G165C (GGC→TGC) | modF ← | fused molybdate transporter subunits of ABC superfamily: ATP‑binding components |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 788,752 | 0 | C | A | 12.1% | 71.5 / 2.4 | 33 | G165C (GGC→TGC) | modF | fused molybdate transporter subunits of ABC superfamily: ATP‑binding components |
| Reads supporting (aligned to +/- strand): ref base C (18/11); new base A (2/2); total (20/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01 | |||||||||||
CAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGC > CP009273/788613‑788894 | cAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGa > 2:273357/1‑145 (MQ=255) aaTCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGaa < 2:361492/145‑1 (MQ=255) ccGCCAGCAGGCCAGCAAACTGGACAACCTCCGGGATCTGATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACGGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACgg < 1:98159/145‑1 (MQ=255) ccagcacgccagcaAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCtc < 2:322148/145‑1 (MQ=255) ccagcacgccagcaAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCAATCGAACGGCtc > 1:16912/1‑145 (MQ=255) ccagcacgccagcaAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCAATCGAACGGCtc > 1:74603/1‑145 (MQ=255) gccagcaAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTCCCAGCGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGa > 1:278652/1‑145 (MQ=255) ccagcaAACTGGACAAACTCCGGGATCTCATCGGAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGaa < 2:207546/145‑1 (MQ=255) cagcaAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACAAGGCGAGTCGCTCAGCCAGCTGCTGACGTAAGGCAACATCCAGGCCATCGAACGGCTCATCAAAAAt > 2:137641/1‑145 (MQ=255) agccAACTGGACCAACGCCGGCATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGGAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATc < 1:330872/145‑1 (MQ=255) aaaCTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCAGCAAGAATcaac > 2:278364/1‑145 (MQ=255) aaCTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATcaaca > 2:24741/1‑145 (MQ=255) gaTCGAAGCGATTGAGCACCAGTACCAGGGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGACAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGACTCCGACATCAgcga > 1:390944/2‑144 (MQ=255) aTCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATGAAGAATCAACAAGTCAGGCTCCGACATCAGCGcc > 1:134564/1‑145 (MQ=255) cAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCAATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGttttt < 2:74603/145‑1 (MQ=255) cAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCAATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGttttt < 2:16912/145‑1 (MQ=255) ggaCCAGGATAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTcg < 1:48685/143‑1 (MQ=255) tACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTcgc > 1:51777/1‑145 (MQ=255) caCCGGACGGAGGTAACGAGCCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGt < 1:79333/144‑1 (MQ=255) cGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGa > 1:333654/1‑145 (MQ=255) aTGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGCACGGCTCATCAGGAATCAACAAGTCAGGCTCCGACATCAGCGCCGGACACAGACGGGTTTTGCGCGTCTCGCCAGTGGAAAGGTAt > 1:140928/1‑145 (MQ=255) aCGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTaaa > 1:197663/1‑145 (MQ=255) gAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGc > 2:403436/1‑145 (MQ=255) gAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACACCAGCGCCTGACACAGCAGGGCTTTTCGCGTCCCGCCAGCGGAAAGGGATGTAAAGc > 1:265143/1‑145 (MQ=255) gCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCCAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCctc > 2:39725/1‑145 (MQ=255) gCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCgtc < 2:149917/145‑1 (MQ=255) gCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCgtc > 1:109543/1‑145 (MQ=255) cGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCga < 2:333654/145‑1 (MQ=255) tGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAAc < 2:278652/145‑1 (MQ=255) gCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAActgct > 2:78064/1‑145 (MQ=255) gCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATGTAAAGCGTCGGTCGAGGAGGGCGGTAATCCCGAAcggct > 2:408781/1‑145 (MQ=255) cacccAGGCCAACGAACGGCACATCAACAATCAACAAGTCAGGCTCCGCCATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTTGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTgcgc < 1:402910/142‑1 (MQ=255) gCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGGGCGTGACAATGCAGGGAACATAGTGAACAGTGTGCGTAATGCTATTTACAGCGTCGACTGAGGAGGGCGGTATTACCGGACTGCTGCGCCAGCtgc > 1:246831/1‑145 (MQ=255) | CAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGC > CP009273/788613‑788894 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |